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{{#ask:[[Category:Publications]] [[Additional label::ORO]] [[Additional label::2016]] |?Has title=Where

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...label::Safranin]] OR [[Additional label::Magnesium Green]] OR [[Additional label::Calcium Green 5N]] |?Has title=Reference

...nt and method::Oxygraph-2k]] [[Additional label::2024-04]] OR [[Additional label::2024-05]] |?Has title=Reference

{{#ask:[[Category:O2k-Workshops]][[Additional label::IOC]] |?Has title=Reference

...and method::O2k-Protocol]] [[Additional label::O2k-Analysis]] [[Additional label::DatLab]] |?Has info=View

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{{#ask:[[Additional label::Phosphate]] |?Has title=Reference

{{#ask:[[Additional label::MitoFit 2022 ROS review]] |?Has info=View

...ent and method::O2k-Protocol]] [[Additional label::O2k-Demo]] [[Additional label::O2k-Core]] |?Has info=View

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...tegory:Publications]] [[Instrument and method::O2k-Protocol]] [[Additional label::O2k-chemicals and media]] |?Has info=View

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{{#ask:[[Category:Publications]] [[Additional label::2014]] |?Has title=Location - Country

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...tegory:Publications]] [[Instrument and method::O2k-Protocol]] [[Additional label::Mt- and marker-enzymes]] |?Has info=View

{{#ask:[[Category:Abstracts]] [[Additional label::MiP2013]] [[Diseases::Diabetes]] |?Has title

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{{#ask:[[Category:Abstracts]] [[Additional label::MiP2013]] [[Stress::Hypoxia]] |?Has title

{{#ask:[[Category:Abstracts]] [[Additional label::MiP2013]] [[Diseases::Aging; senescence]] |?Has title

{{#ask:[[Additional label::Mitochondrial outer membrane]] |?Has title=Reference

...ategory:Publications]] [[Instrument and method::Oxygraph-2k]] [[Additional label::Chlamydomonas]] |?Has title=Reference

{{#ask:[[Category:Abstracts]] [[Additional label::MiP2013]] [[Diseases::Cancer]] |?Has title

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|?Has status=Status O2k {{#ask:[[Category:Publications]] [[Additional label::CN]]

{{#ask:[[Category:Publications]] [[Additional label::Elamipretide]] |?Has title=Reference

|?Has status=Status O2k {{#ask:[[Category:Publications]] [[Additional label::JP]]

{{#ask:[[Category:Abstracts]] [[Additional label::MiP2013]] [[Tissue and cell::Heart]] |?Has title

|?Has abbr=Abbreviation {{#ask:[[Category:Publications]] [[Additional label::Preprints]]

{{#ask:[[Category:Abstracts]] [[Additional label::MiP2013]] [[Tissue and cell::Skeletal muscle]] |?Has title

{{#ask:[[Category:Abstracts]] [[Additional label::MiP2013]] [[Tissue and cell::Nervous system]] |?Has title

...ategory:Publications]] [[Instrument and method::Oxygraph-2k]] [[Additional label::PBI-Shredder]] |?Has title=Reference

...ategory:Publications]] [[Instrument and method::Oxygraph-2k]] [[Additional label::MgG]] |?Has title=Reference

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...ategory:Publications]] [[Instrument and method::Oxygraph-2k]] [[Additional label::Photosynthesis]] |?Has title=Reference

...ategory:Publications]] [[Instrument and method::Oxygraph-2k]] [[Additional label::PBMCs]] |?Has title=Reference

{{#ask:[[Category:Abstracts]] [[Additional label::MiP2013]] [[MiP area::Exercise physiology;nutrition;life style]] |?Has title

{{#ask:[[Additional label::MitoFit 2022 NADH]] |?Has title=Reference

...ategory:Publications]] [[Instrument and method::Oxygraph-2k]] [[Additional label::Tissue normoxia]] |?Has title=Reference

...ategory:Publications]] [[Instrument and method::Oxygraph-2k]] [[Additional label::CaG]] |?Has title=Reference

|?Has status=Status O2k {{#ask:[[Category:Publications]] [[Additional label::KR]]

|?Has abbr=Abbreviation {{#ask:[[Category:Publications]] [[Additional label::BEC]]

{{#ask:[[Category:Publications]] [[Additional label::ORO]] [[Additional label::2019]] |?Has title=Where

...ategory:Publications]] [[Instrument and method::Oxygraph-2k]] [[Additional label::PBMC]] |?Has title=Reference

...ategory:Publications]] [[Instrument and method::Oxygraph-2k]] [[Additional label::Leukemia]] |?Has title=Reference

{{#ask:[[Additional label::Permeabilized tissue]] |?Has title=Reference

...as an [[elementary entity]]. The term entity with symbol ''X'', therefore, has a general meaning, including but not limited to elementary entities ''U''<s {{#ask:[[Additional label::Entity]]

|?Has status=Status O2k {{#ask:[[Category:Publications]] [[Additional label::TW]]

{{#ask:[[Additional label::LEDR]] |?Has title=Reference

{{#ask:[[Additional label::Mitochondrial inner membrane]] |?Has title=Reference

{{#ask:[[Category:Publications]] [[Additional label::MitoEAGLE terminology]] |?Has title=Reference

...tegory:Publications]] [[Instrument and method::O2k-Protocol]] [[Additional label::MitoPathways]] |?Has info=View

{{#ask:[[Additional label::Isolated mitochondria]] |?Has title=Reference

...cription='''DatLab 8''': Set a [[Power O2k Numbers | Power-O2k number]] to label the O2k in a Power O2k-Lab. ...t. This option is enabled only when an O2k is connected but no measurement has been started yet. The Power-O2k number is displayed, for example, in the [[

{{#ask:[[Category:Publications]] [[Additional label::ORO]] [[Additional label::2020]] |?Has title=Where

{{#ask:[[Additional label::MitoFit 2021 MgG]] |?Has title=Reference

{{#ask:[[Additional label::Format]] |?Has title=Reference

{{#ask:[[Additional label::Charge number]] |?Has title=Reference

|?Has abbr=Abbreviation {{#ask:[[Category:Publications]] [[Additional label::Gentle Science]]

{{#ask:[[Category:Publications]] [[Additional label::Blood cell preparation]] |?Has title=Reference

{{#ask:[[Category:Publications]] [[Additional label::Made history]] |?Has title

{{#ask:[[Additional label::Comparison of respirometric methods]] |?Has title=Reference

:::* [[Digitonin]] is a natural product and thus the effective concentration has to be determined by titrations for every batch. The optimum effective digit ...:O2k-Protocol]] [[Additional label::SUIT-010 O2 ce-pce D008]] [[Additional label::SUIT-010]]

{{#ask:[[Category:Publications]] [[Additional label::H2]] |?Has title=Reference

[[additional label:: POS 1983]] |?Has info=View

{{#ask:[[Category:Publications]] [[Additional label::ALAS events]] |?Has title=Where

{{#ask:[[Category:Publications]] [[Additional label::Comorbidity]] |?Has title=Reference

[[File:NextGenO2k logo.jpg|80px|left]] Supported by project NextGen-O2k which has received funding from the European Union’s Horizon 2020 research and inno {{#ask:[[Additional label::O2k-brief]]

{{#ask:[[Additional label::Substrate-uncoupler-inhibitor titration]] |?Has title=Reference

{{#ask:[[Category:Publications]] [[Additional label::O2k-Core]] |?Has info=View

{{#ask:[[Additional label::Alternative oxidase]] |?Has title=Reference

{{#ask:[[Category:Publications]] [[Additional label::Resveratrol]] |?Has title=Reference

{{#ask:[[Additional label::Flux]] |?Has title=Reference

{{#ask:[[Additional label::Permeabilized cells]] |?Has title=Reference

...l screening ('genomic fingerprints'), functional mitochondrial diagnostics has been largely neglected so far in clinical trials. Diagnostic services remai {{#ask:[[Category:Publications]] [[Additional label::Mitochondrial Diagnostics events]]

{{#ask:[[Additional label::Mitochondrial preparations]] |?Has title=Reference

:::: Supported by project NextGen-O2k which has received funding from the European Union’s Horizon 2020 research and inno {{#ask:[[Additional label::MitoFit 2021 MgG]]

{{#ask:[[Category:Publications]] [[Additional label::Blebbistatin]] |?Has title=Reference

{{#ask:[[Category:Publications]] [[Additional label::IOC]] [[Additional label::Next]] |?Has info=More details

{{#ask:[[Additional label::MitoEAGLE muscle buffer]] [[Additional label::MiR05]] |?Has title=Reference

{{#ask:[[Category:Publications]] [[Additional label::MitoFitPublication]] |?Has title=

{{#ask:[[Additional label::IUPAC]] |?Has title=Reference

...Category:Publications]] [[Instrument and method::O2k-Manual]] [[Additional label::O2k-MultiSensor]] |?Has title=Section

:::: The optimum concentration of an uncoupler has to be determined for every biological system. It varies with incubation med ...Physiol]]). To evaluate the optimum concentration, an uncoupler titration has to be performed initially. For subsequent application series, we recommend

{{#ask:[[Additional label::Flow]] |?Has title=Reference

{{#ask:[[Category:Publications]] [[Additional label::O2k-Network Award]] |?Has title=Location - Country - IOC

|?Has title=Reference {{#ask:[[Additional label::Complex I]]

|?Has abbr=Abbreviation | ?Has abbr=Abbreviation

{{#ask:[[Category:Publications]] [[Additional label::1PM;2D;3G;4S;5U;6Rot-]] |?Has title=Reference

...more details see: [[Komlodi 2021 MitoFit AmR-O2]]. The respiration medium has to be choosen carefully since the [[Amplex UltraRed]] assay leads to artefa ...tegory:Publications]] [[Instrument and method::O2k-Protocol]] [[Additional label::SUIT-013]]

:::: The notion of respiratory control ratios has advanced far beyond the classical [[respiratory acceptor control ratio]] '' |?Has abbr=Abbreviation

{{#ask:[[Additional label::Tissue homogenate]] |?Has title=Reference

{{#ask:[[Additional label::Cell respiration]] |?Has title=Reference

|?Has title=Reference ...[[Category:Abstracts]] [[Instrument and method::Oxygraph-2k]] [[Additional label::Environmental Physiology; Toxicology]]

:::: The Institute for Human Genetics has been equipped with 2 O2ks for the duration of the project. {{#ask:[[Category:Publications]] [[Additional label::TissueModels events]]

{{#ask:[[Additional label::Coupling-control ratio]] |?Has title=Reference

{{#ask:[[Category:Publications]] [[Additional label::MitoEAGLEPublication]] |?Has title=Reference

{{#ask:[[Additional label::Normalization of rate]] |?Has title=Reference

{{#ask:[[Category:Publications]] [[Additional label::1PM;2D;3G;4U;5S;6Rot-]] |?Has title=Reference

...rther details see: [[Komlodi 2021 MitoFit AmR-O2]]. The respiration medium has to be chosen carefully, since the [[Amplex UltraRed]] assay leads to artefa ...tegory:Publications]] [[Instrument and method::O2k-Protocol]] [[Additional label::SUIT-013]]

...:Publications]] [[Additional label::SUIT-027 O2 ce-pce D065]] [[Additional label::SUIT-027]] | ?Has title=Reference

{{#ask:[[Additional label::Chemical background]] |?Has title=Reference

{{#ask:[[Additional label::Dimension]] |?Has title=Reference

::* This protocol has been designed to study the effect of the [[Carrier control titrations|carri ::- The concentration of [[oligomycin]] has to be carefully tested to avoid inhibitory effects in the evaluation of the

...Publications]] [[Additional label::SUIT-009 O2 ce-pce D016]] [[Additional label::O2k-Protocol]] [[Instrument and method::O2k-Protocol]] | ?Has title=Reference

...tegory:Publications]] [[Instrument and method::Oxygr<aph-2k]] [[Additional label::Alert2024]] |?Has title=Reference

...gory:Publications]] [[Additional label::SUIT-025 O2 mt D057]] [[Additional label::SUIT-025]] | ?Has title=Reference

{{#ask:[[Additional label::Normalization of rate]] |?Has title=Reference

...al processes, such as production of reactive oxygen species, where the ΔpH has an important role. {{#ask:[[Additional label::MitoFit 2022 pmF]]

{{#ask:[[Additional label::Residual oxygen consumption]] |?Has title=Reference

:::* This protocol has been designed to study the inhibitory effect of the Amplex UltraRed assay o :::- The concentration of [[oligomycin]] has to be carefully tested to avoid inhibitory effects in the evaluation of the

|abstract=The [[International System of Units]], the SI, has been used around the world as the preferred system of units, the basic lang {{#ask:[[Additional label::International System of Units]]

::::* [[SUIT-022 O2 ce D051]] has to be used in parallel to obtain complete information of the contribution o {{#ask:[[Category:Publications]] [[Additional label::SUIT-023 O2 ce D053]]

{{#ask:[[Additional label::International System of Units]] |?Has title=Reference

...com/blog/how-to-choose-long-tail-keywords/?kw=&cmp=DE_SRCH_DSA_Blog_SEO_EN&label=dsa_pagefeed&Network=g&Device=c&utm_content=431517091371&kwid=aud-296306606 ::::* Add the keywords in the BEC article uner 'Additional label', separated by commas, and save the page. This may be the long list of keyw

...steps of the citric acid cycle (Krebs cycle, tricarboxylic acid TCA cycle) has been characterized by the kinetic and regulatory properties of the enzymes {{#ask:[[Additional label::MitoFit2022rTCA]]

...N'' objects measured on top of each other. Therefore, the height per human has the unit [m·x^-1] (compare [[body mass]] [kg·x^-1]). ...p>2</sup> (dimension of area) and ''H''³ (dimension of volume), has the corresponding units [m²·x^-1] (area per object) a

...of O₂, ''M''_r,O2, is 32 (or 31.9988). The element O has 8 protons, 8 neutrons and 8 electrons. In the figure, the two electrons in {{#ask:[[Additional label::Oxygen]]

{{#ask:[[Category:Publications]] [[Additional label::SUIT-018 AmR mt D031]] [[Instrument and method::O2k-Protocol]] | ?Has title=Reference

...tion force and time for separation of mitochondria. The appropriate method has to be selected to meet specific needs for purity and functionality of isola ...tegory:Publications]] [[Instrument and method::O2k-Protocol]] [[Additional label::Mt-Preparation]]

::::* [[SUIT-023 O2 ce D053]] has to be used in parallel to obtain complete information of the contribution o {{#ask:[[Category:Publications]] [[Additional label::SUIT-022 O2 ce D051]]

...Category:Publications]] [[Instrument and method::O2k-Manual]] [[Additional label::DatLab2]] |?Has title=Section

{{#ask:[[Additional label::Amount of substance]] |?Has title=Reference

...apacity induced by addition of uncoupler, the optimal concentration of Omy has to be determined. For mt-membrane potential analysis and calculation, visit :::- Omy concentration has to be determined. Higher concentrations of Omy may inhibit the ET state.

...tity''': "property of a phenomenon, body, or substance, where the property has a magnitude that can be expressed as a number and a reference. .. The gener {{#ask:[[Additional label::Quantity]]

...support of the [https://www.fwf.ac.at/en/ FWF Austrian Science Fund], SPIN has been refunded and is now entering its 4th Funding Period. {{#ask:[[Category:Publications]] [[Additional label::SPIN]]

...less than 2 L, such that the volume ''V''_S of 1 L pure ethanol has diminished to a smaller volume ''V''_s of ethanol in the mixture; {{#ask:[[Additional label::Density]]

This protocol has been designed to study the H₂O₂ production in various ...we suggest not to go under ~100 µM O₂. [[SUIT-018 AmR mt D041]] has been designed to study O₂ dependence of H₂O<sub>2</su

{{#ask:[[Category:Publications]] [[Additional label::Oroboros Diagnostics events]] |?Has title=Where

...combination, these biomarkers can serve as a valuable tool for sensitive, label-free identification of changes in specific metabolic pathways and character

|description=The term '''system''' has a variety of meanings and dictionary definitions in different contexts, ''e {{#ask:[[Additional label::System]]

{{#ask:[[Additional label::Coupling control]] |?Has title=Reference

...DL-Protocol User (*.DLPU)|DLPU]]. However, the addition of [[oligomycin]] has to be carefully tested to avoid inhibitory effects in the evaluation of the {{#ask:[[Category:Publications]] [[Additional label::SUIT-003 O2 ce D062]]

:::- The addition of [[oligomycin]] has to be carefully tested to avoid inhibitory effects in the evaluation of the {{#ask:[[Category:Publications]] [[Additional label:SUIT-003 O2 ce D018]]

...city induced upon addition of uncoupler, the required concentration of Omy has to be determined. :::- [[Oligomycin| Oligomycin]] concentration has to be determined. Higher concentrations of Oligomycin may inhibit the [[Ele

{{#ask:[[Additional label::MitoFit 2021.5 PB]] |?Has title=Reference

:::: The MitoKIN project has three major aims: :::: The Institute of Biochemistry has been equipped with 3 O2ks for the duration of the project.

...ory:Publications]] [[Additional label::SUIT-001 O2 pfi D002]] [[Additional label::SUIT-001]] | ?Has title=Reference

::: - In some cell lines e.g. HEK293T, P has not any effect because of the downregulation of the N-linked pathway. {{#ask:[[Category:Abstracts]] [[Additional label::SUIT-009 O2 mt D015]] [[Instrument and method::O2k-Protocol]]

:::- [[Oligomycin| Oligomycin]] concentration has to be determined. Higher concentrations of Omy may inhibit the ET state. {{#ask:[[Category:Publications]] [[Additional label::SUIT-020 Fluo mt D033]] [[Instrument and method::O2k-Protocol]]

...ory:Publications]] [[Additional label::SUIT-002 O2 pfi D006]] [[Additional label::SUIT-002]] | ?Has title=Reference

{{#ask:[[Additional label::Adenine nucleotides]] |?Has title=Reference

{{#ask:[[Category:Publications]] [[Additional label::SUIT-009 AmR mt D021]] [[Instrument and method::O2k-Protocol]] | ?Has title=Reference

...y ∼30% at Complex I and II of the electron transport system. Additionally, label-free proteomic analysis uncovered various potential novel targets of miR-23

...we suggest not to go under ~100 µM O₂. [[SUIT-018 AmR mt D041]] has been designed to study O₂ dependence of H₂O<sub>2</su {{#ask:[[Category:Publications]] [[Additional label::SUIT-009 AmR ce-pce D019]]

...tate induced upon addition of uncoupler, the required concentration of Omy has to be assessed by the [https://bioblast.at/index.php/Oligomycin Omy titra :::- [[Oligomycin| Oligomycin]] concentration has to be determined. Higher concentrations of Oligomycin may inhibit the [[Ele

{{#ask:[[Additional label::MitoFit 2021 AmR-O2]] |?Has title=Reference

{{#ask:[[Additional label::Oxidative phosphorylation]] |?Has title=Reference

...tegory:Publications]] [[Instrument and method::O2k-Protocol]] [[Additional label::SUIT-031]] |?Has title=Reference

{{#ask:[[Category:Publications]] [[Additional label::SUIT-032 O2 mt D109]] [[Instrument and method::O2k-Protocol]] | ?Has title=Reference

...l homogenization it is difficult to know when sufficient tissue disruption has occurred - overhomogenization! {{#ask:[[Category:Publications]] [[Additional label::PBI-Shredder]]

{{#ask:[[Category:Publications]] [[Additional label::SUIT-006 O2 mt D108]] [[Instrument and method::O2k-Protocol]] | ?Has title=Reference

{{#ask:[[Category:Publications]] [[Additional label::SUIT-003 O2 ce D039]] |?Has title=Reference

{{#ask:[[Category:Publications]] [[Additional label::SUIT-034_O2_mt_D111]] [[Instrument and method::O2k-Protocol]] | ?Has title=Reference

...of studying intact mitochondria is ''in vitro'' technique, and although it has many advantages, in some aspects it is far from being representative when i |additional=Label,

{{#ask:[[Category:Publications]] [[Additional label::SUIT-033 O2 mt D110]] [[Instrument and method::O2k-Protocol]] | ?Has title=Reference

...tegory:Publications]] [[Instrument and method::O2k-Protocol]] [[Additional label::SUIT-017 O2 pfi D049]] |?Has title=Reference

...tate induced upon addition of uncoupler, the required concentration of Omy has to be determined. Addition of Omy results in hyperpolarisation owing to the :::- [[Oligomycin| Oligomycin]] concentration has to be determined. Higher concentrations of Omy may inhibit the ET state.

...DL-Protocol User (*.DLPU)|DLPU]]. However, the addition of [[oligomycin]] has to be carefully tested to avoid inhibitory effects in the evaluation of the {{#ask:[[Category:Publications]] [[Additional label::SUIT-003 O2 ce D050]]

...state induced upon addition of uncoupler, the optimal concentration of Omy has to be determined. ...we suggest not to go under ~100 µM O₂. [[SUIT-018 AmR mt D041]] has been designed to study O₂ dependence of H₂O<sub>2</su

{{#ask:[[Category:Publications]] [[Additional label::O2k-Core]] |?Has info=View

...ded to the chamber) in a wide variety of species and tissues. The protocol has been designed to test the mitochondrial respiration at low oxygen levels an {{#ask:[[Category:Publications]] [[Additional label::SUIT-018]] [[Instrument and method::O2k-Protocol]]

{{#ask:[[Category:Publications]] [[Additional label:SUIT-007 O2 pce D030]] | ?Has title=Reference

...:Publications]] [[Additional label::SUIT-001 O2 ce-pce D004]] [[Additional label::SUIT-001]] | ?Has title=Reference

...gory:Publications]] [[Additional label::SUIT-001 O2 mt D001]] [[Additional label::SUIT-001]] | ?Has title=Reference

:::* [[Digitonin]] is a natural product and thus the effective concentration has to be determined by titrations for every batch. The optimum effective digit ...tegory:Publications]] [[Instrument and method::O2k-Protocol]] [[Additional label::SUIT-010]]

...gory:Publications]] [[Additional label::SUIT-002 O2 mt D005]] [[Additional label::SUIT-002]] | ?Has title=Reference

...l separation technique in several research and clinical areas. However, it has been suggested that the force acting upon cells undergoing acoustophoresis ...human leukocytes and thrombocytes to explore if acoustophoretic processing has adverse effects.

:::- The concentration of [[oligomycin]] has to be carefully tested to avoid inhibitory effects in the evaluation of the {{#ask:[[Category:Publications]] [[Additional label::SUIT-003 O2 ce-pce D020]]

:::- The addition of [[oligomycin]] has to be carefully tested to avoid inhibitory effects in the evaluation of the {{#ask:[[Category:Publications]] [[Additional label:SUIT-003 O2 ce D012]]

{{#ask:[[Additional label::Gnaiger 2024 MitoFit]] |?Has title=Reference

...:Publications]] [[Additional label::SUIT-002 O2 ce-pce D007]] [[Additional label::SUIT-002]] | ?Has title=Reference

...If mt-area is determined by electron microscopy, the statistical challenge has to be met to convert area into a volume. When fluorescent dyes are used as {{#ask:[[Additional label::Mitochondrial marker]]

...be inhibited by [[oligomycin]]. The final concentration of [[oligomycin]] has to be carefully optimized for various cell types, to minimize the inhibitor :::- To obtain [[LEAK respiration]], the concentration of [[oligomycin]] has to be carefully tested to avoid inhibitory effects in the evaluation of the

:::- Omy concentration has to be determined if used. Higher concentrations of Omy may inhibit the ET s {{#ask:[[Category:Publications]] [[Additional label::SUIT-006 Q mt D071]]

{{#ask:[[Additional label::Volume]] |?Has title=Reference

Thus the Avogadro constant ''N''_A has the SI unit 'per mole' [mol^-1], but more strictly the unit for c {{#ask:[[Additional label::Avogadro constant]]

Select a channel and edit Channel label and Unit. In the Amp calibration window a drop-down menu allows to select m DatLab performs a new linear regression after any field has been edited and displays the sensitivity and intercept (3a). Calibration pa

...Publications]] [[Additional label::SUIT-002 O2 ce-pce D007a]] [[Additional label::SUIT-002]] |?Has title=Reference

...tegory:Publications]] [[Instrument and method::O2k-Protocol]] [[Additional label::SUIT-024]] |?Has title=Reference

...mpirical, objective measure of health, and BMI as an arbitrary, subjective label for categorizing the population. I further consider how BMI is empowered as ::::* 'BMI has helped define the obesity epidemic due to its ease of collection, analysis,

...oisoi N, Chakrabarti L (2022) Exercise alters mitochondrial physiology and has age-specific impacts on the fitness and lifespan of ''D. melanogaster''. '' ...anges to mitochondrial physiology through high-resolution respirometry and label-free mass spectrometry. Exercise in late life extends the lifespan of male

...tabolism, the volume of oxygen gas, ''V''_O₂(g) [mL], has to be converted into an amount of dissolved oxygen, ''n''<sub>O₂ {{#ask:[[Additional label::VO2max]]

{{#ask:[[Additional label::Concentration]] |?Has title=Reference

{{#ask:[[Additional label::Flux control ratio]] |?Has title=Reference

:::- The addition of [[oligomycin]] has to be carefully tested to avoid inhibitory effects in the evaluation of the {{#ask:[[Category:Publications]] [[Additional label::SUIT-003 Ce1;ce2Omy;ce3U;ce4Rot;ce5S;ce6Ama]]

{{#ask:[[Category:Publications]] [[Additional label::SUIT-006 O2 pce D029]] | ?Has title=Reference

...tegory:Publications]] [[Instrument and method::O2k-Protocol]] [[Additional label::SUIT-022]] |?Has title=Reference

...tegory:Publications]] [[Instrument and method::O2k-Protocol]] [[Additional label::SUIT-023]] |?Has title=Reference

{{#ask:[[Additional label::Oxygen flux]] |?Has title=Reference

{{#ask:[[Category:Publications]] [[Additional label::SUIT-018]] [[Additional label::SUIT-018 AmR mt D031]] [[Instrument and method::O2k-Protocol]] | ?Has title=Reference

“The International System of Units, the SI, has been used around the world as the preferred system of units, the basic lang {{#ask:[[Additional label::MitoFit 2020.4]]

{{#ask:[[Additional label::Mitochondria]] |?Has title=Reference

...tegory:Publications]] [[Instrument and method::O2k-Protocol]] [[Additional label::SUIT-004]] |?Has title=Reference

{{#ask:[[Category:Publications]] [[Additional label::SUIT-015 O2 pti D043]] | ?Has title=Reference

:::- Omy concentration has to be determined if used. Higher concentrations of Omy may inhibit the ET s {{#ask:[[Category:Publications]] [[Additional label::SUIT-006 Q ce-pce D073]]

{{#ask:[[Additional label::Molar mass]] |?Has title=Reference

|abstract=Conventionally, the 'electron transport chain' has been considered as the sequence of membrane-bound respiratory complexes, ma ...chain, ETC) is a misnomer. Understanding mitochondrial respiratory control has suffered greatly from this inappropriate terminology, although textbooks us

:::- Omy concentration has to be determined if used. Higher concentrations of Omy may inhibit the ET s {{#ask:[[Category:Publications]] [[Additional label::SUIT-006 NADH mt D084]] [[Instrument and method::O2k-Protocol]]

{{#ask:[[Additional label::MitoFit 2021 PLT]] |?Has title=Reference

{{#ask:[[Category:Publications]] [[Additional label::SUIT-003 pH ce-pce D037]] |?Has title=Reference

{{#ask:[[Additional label::MitoFit 2021 Proline]] |?Has title=Reference

{{#ask:[[Additional label::MitoFit2022QC]] |?Has title=Reference

:::- When evaluating the additive effect of the N- and S-pathway, it has to be considered that NS_''P''- and NS_''E''-capacities {{#ask:[[Category:Publications]] [[Additional label::SUIT-014 O2 pfi D042]]

{{#ask:[[Additional label::Respiratory complexes]] |?Has title=Reference

{{#ask:[[Category:Publications]] [[Additional label::SUIT-006 O2 mt D047]] | ?Has title=Reference

...DL-Protocol User (*.DLPU)|DLPU]]. However, the addition of [[oligomycin]] has to be carefully tested to avoid inhibitory effects in the evaluation of the {{#ask:[[Category:Publications]] [[Additional label::SUIT-003 AmR ce D060]] [[Instrument and method::O2k-Protocol]]

...dels is equivalent to the anti-HEV potency of ribavirin, a widely used off-label treatment for patients with chronic HEV. Mechanistically, we found that thi

{{#ask:[[Category:Publications]] [[Additional label::SUIT-003 O2 ce-pce D037]] |?Has title=Reference

...tegory:Publications]] [[Instrument and method::O2k-Protocol]] [[Additional label::SUIT-017]] |?Has title=Reference

{{#ask:[[Category:Publications]] [[Additional label::SUIT-003 O2 ce-pce D038]] |?Has title=Reference

{{#ask:[[Additional label::MitoFit 2021 CoQ]] |?Has title=Reference

:::- Omy concentration has to be determined if used. Higher concentrations of Omy may inhibit the ET s {{#ask:[[Category:Publications]] [[Additional label::SUIT-031 Q mt D072]]

{{#ask:[[Additional label::PLoSONE2022ace-sce]] |?Has title=Reference

:::: Supported by project NextGen-O2k which has received funding from the European Union’s Horizon 2020 research and inno {{#ask:[[Additional label::MitoFit 2021 AmR-O2]]

:::- Omy concentration has to be determined if used. Higher concentrations of Omy may inhibit the ET s {{#ask:[[Category:Publications]] [[Additional label::SUIT-031 Q mt D072]]

|description='''Curcumin''' has been shown to possess significant anti-inflammatory, anti-oxidant, anti-car {{#ask:[[Category:Publications]] [[Additional label::Curcumin]]

{{#ask:[[Additional label::MitoFit 2021 PLT]] |?Has title=Reference

...tegory:Publications]] [[Instrument and method::O2k-Protocol]] [[Additional label::SUIT-007]] |?Has title=Reference

{{#ask:[[Category:Publications]] [[Additional label::SUIT-006 O2 mt D022]] | ?Has title=Reference

...tegory:Publications]] [[Instrument and method::O2k-Protocol]] [[Additional label::SUIT-017 O2 mt D]] |?Has title=Reference

{{#ask:[[Category:Publications]] [[Additional label::SUIT-031]] | ?Has title=Reference

...Category:Publications]] [[Instrument and method::O2k-Manual]] [[Additional label::O2k-MultiSensor]] |?Has title=Section

...tegory:Publications]] [[Instrument and method::O2k-Protocol]] [[Additional label::SUIT-015]] |?Has title=Reference

...tegory:Publications]] [[Instrument and method::O2k-Protocol]] [[Additional label::SUIT-025]] |?Has title=Reference

:::- The concentration of [[oligomycin]] has to be carefully tested to avoid inhibitory effects in the evaluation of the {{#ask:[[Category:Publications]] [[Additional label::SUIT-003 O2 ce D009]]

{{#ask:[[Category:Publications]] [[Additional label::SUIT-031]] | ?Has title=Reference

{{#ask:[[Category:Publications]] [[Additional label::Metformin]] |?Has title=Reference

{{#ask:[[Category:Publications]] [[Additional label::SUIT-004 O2 pfi D010]] | ?Has title=Reference

...tegory:Publications]] [[Instrument and method::O2k-Protocol]] [[Additional label::SUIT-027]] |?Has title=Reference

{{#ask:[[Category:Publications]] [[Additional label::SUIT-018 AmR ce-pce D068]] [[Instrument and method::O2k-Protocol]] | ?Has title=Reference

:::- When evaluating the additive effect of the N- and S-pathway, it has to be considered that NSP- and NSE-capacities can only be compared with NP- {{#ask:[[Category:Publications]] [[Additional label::SUIT-008 O2 pce D025]]

{{#ask:[[Additional label::OXPHOS-capacity]] |?Has title=Reference

...tegory:Publications]] [[Instrument and method::O2k-Protocol]] [[Additional label::SUIT-012 O2 mt D027]] |?Has title=Reference

...tegory:Publications]] [[Instrument and method::O2k-Protocol]] [[Additional label::SUIT-023]] |?Has title=Reference

...prodrug hydrolysis, formaldehyde is released which, at high concentration, has an inhibitory effect on glycolysis ([https://www.sciencedirect.com/science/ {{#ask:[[Additional label::MitoKit-CII]]

:::- When evaluating the additive effect of the N- and S-pathway, it has to be considered that NSP- and NSE-capacities can only be compared with NP- {{#ask:[[Category:Publications]] [[Additional label::SUIT-008 O2 pfi D014]]

...tegory:Publications]] [[Instrument and method::O2k-Protocol]] [[Additional label::SUIT-005]] |?Has title=Reference

...2]]): A prominent case of ambiguity in the grey zone between types 1 and 2 has been uniquely demonstrated by analysis of the popular notion of 'oxidative ...0 Chapter 8]]): The ambiguous use (type 1) of the terms force and pressure has deep consequences on the enigmatic concept of non-ohmic flux-force relation

{{#ask:[[Category:Publications]] [[Additional label::SUIT-032 NADH mt D078]] [[Instrument and method::O2k-Protocol]] | ?Has title=Reference

...tegory:Publications]] [[Instrument and method::O2k-Protocol]] [[Additional label::SUIT-012 ce-pce D052]] |?Has title=Reference

{{#ask:[[Category:Publications]] [[Additional label::SUIT-019 O2 pfi D045]] | ?Has title=Reference

...tegory:Publications]] [[Instrument and method::O2k-Protocol]] [[Additional label::SUIT-005 O2 pfi D011]] |?Has title=Reference

:::- When evaluating the additive effect of the N- and S-pathway, it has to be considered that NS_''P''- and NS_''E''-capacities ...tegory:Publications]] [[Instrument and method::O2k-Protocol]] [[Additional label::SUIT-014]]

:::: Supported by project NextGen-O2k which has received funding from the European Union’s Horizon 2020 research and inno {{#ask:[[Additional label::MitoFit 2021 CoQ]]

...tegory:Publications]] [[Instrument and method::O2k-Protocol]] [[Additional label::SUIT-019]] |?Has title=Reference

{{#ask:[[Category:Publications]] [[Additional label::SUIT-034_NADH_mt_D082]] [[Instrument and method::O2k-Protocol]] | ?Has title=Reference

...pes. Further strengthening our brand recognition, a communication strategy has been put into place to bring an updated corporate identity with a new look {{#ask:[[Category:Publications]] [[Additional label::NextGen-O2k]]

{{#ask:[[Category:Publications]] [[Additional label::SUIT-033 NADH mt D081]] [[Instrument and method::O2k-Protocol]] | ?Has title=Reference

:::- For additive effect evaluation of N- and S-pathways, it has to be considered that NS_''P'' and NS_''E'' capacities ...tegory:Publications]] [[Instrument and method::O2k-Protocol]] [[Additional label::SUIT-008]]

...itory effect on the [[Electron transfer pathway|electron transfer system]] has to be studied to avoid an underestimation of the [[ET capacity]]. SUIT-003 ::::* [[SUIT-003 AmR ce D017]] this protocol has been designed to study the H₂O₂ production in various

{{#ask:[[Category:Abstracts]] [[Additional label::MiP2013]] |?Has title

{{#ask:[[Category:Publications]] [[Additional label::SUIT-008 O2 mt D026]] | ?Has title=Reference

...tegory:Publications]] [[Instrument and method::O2k-Protocol]] [[Additional label::SUIT-016 O2 pfi D044]] |?Has title=Reference

{{#ask:[[Category:Publications]] [[Additional label::Malic enzyme]] |?Has title=Reference

{{#ask:[[Category:Publications]] [[Additional label::EU-METAHEART events]] |?Has title=Where

...y the charge number of the proton (''z''_H⁺ = 1), and has the form [1] {{#ask:[[Additional label::Protonmotive force]]

...be changed by the user in a DL-Protocol. However, with DatLab 7.4 the user has more flexibility and the fixed sequence of events and marks may be edited a ...abel, go to menu [Oroboros O2k]\O2k channel labels and set the new channel label as desired.

...tegory:Publications]] [[Instrument and method::O2k-Protocol]] [[Additional label::SUIT-016]] |?Has title=Reference

{{#ask:[[Category:Publications]] [[Additional label::SUIT-029 O2 mt D066]] |?Has title=Reference

{{#ask:[[Category:Publications]] [[Additional label::IOC]] [[Additional label::O2k-Network]] |?Has title=Location - Country - IOC

{{#ask:[[Additional label::Body mass]] |?Has title=Reference

{{#ask:[[Additional label::MitoFit2022Hypoxia]] |?Has title=Reference

...tegory:Publications]] [[Instrument and method::O2k-Protocol]] [[Additional label::SUIT-012]] |?Has title=Reference

...iable approach. Low-load blood flow restricted resistance exercise (BFRRE) has emerged as a time-effective low-load alternative to stimulate myofibrillar ...xide (D₂O) was continuously administered to the participants to label newly synthesized skeletal muscle mitochondrial proteins. Mitochondrial res

...neral (pharmacological or mechanical) [[uncoupled respiration]], to give a label to an effort to reach the state of maximum uncoupler-activated respiration ...m general (pharmacological or mechanical) uncoupled respiration, to give a label to an effort to reach the ''fully uncoupled'' (non-coupled) state without i

{{#ask:[[Category:Publications]] [[Additional label::BMI]] |?Has title=Reference

.... Therefore, pressure as the product of isomorphic force and free activity has the unique unit pascal, [Pa] = [J∙m^-3] = [J∙MU^-1] {{#ask:[[Additional label::Pressure]]

...tegory:Publications]] [[Instrument and method::O2k-Protocol]] [[Additional label::SUIT-028]] |?Has title=Reference

...sample density applied is unusual, double-check if the correct information has been entered into the [[Sample - DatLab |Sample]] window [F3], and check fo :::: [[Marks - DatLab |Marks]] are set to label steady-state sections of the plot of [[oxygen flux]] or [[oxygen flow]]. Nu

{{#ask:[[Additional label::Sample]] |?Has title=Reference

{{#ask:[[Category:Publications]] [[Additional label::SUIT-018]] [[Instrument and method::O2k-Protocol]] | ?Has title=Reference

:::- When evaluating the additive effect of the N- and S-pathway, it has to be considered that NS_''P''- and NS_''E''-capacities {{#ask:[[Category:Publications]] [[Additional label::SUIT-011 O2 pfi D024]]

{{#ask:[[Additional label::ATP]] |?Has title=Reference

{{#ask:[[Category:Publications]] [[Additional label::SUIT-001 O2 ce-pce D003]] | ?Has title=Reference

...evealed contractile failure at nine but not 3 weeks after ischaemia whilst label-free quantitative proteomics identified an increase in proteins promoting a

{{#ask:[[Category:Publications]] [[Additional label::IOC]] [[Additional label::Next]] |?Has title=Location - Country - IOC

:::- When evaluating the additive effect of the N- and S-pathway, it has to be considered that NS_''P''- and NS_''E''-capacities ...tegory:Publications]] [[Instrument and method::O2k-Protocol]] [[Additional label::SUIT-011]]

{{#ask:[[Additional label::Publication efficiency]] |?Has title=Reference

...ing concentration of ADP, which is obscured by any linear regression. This has caused in the past a tremendous underestimation of the apparent Km for ADP, {{#ask:[[Additional label::ADP]]

...imated to be ≈1:8500. Moreover, dysfunction of mitochondrial OXPHOS system has emerged as a key factor in a myriad of “common” diseases, including neu {{#ask:[[Category:Publications]] [[Additional label::REMIX]]

...ity is underestimated. The addition of cytochrome ''c'' (2c), if stability has not yet been reached in the OXPHOS state, leads to an overestimation of the {{#ask:[[Additional label::Steady state]]

{{#ask:[[Category:Publications]] [[Additional label::BMI-cutoff]] |?Has title=Reference

{{#ask:[[Category:Publications]] [[Additional label::SUIT-006 MgG mt D055]] [[Instrument and method::O2k-Protocol]] | ?Has title=Reference

{{#ask:[[Category:Publications]] [[Additional label::SUIT-006 MgG ce-pce D085]] [[Instrument and method::O2k-Protocol]] | ?Has title=Reference

{{#ask:[[Additional label::BEC2021.5]] |?Has title=Reference

...red important connections between neurological and metabolic disorders. It has been shown that brain function is highly influenced by the presence of meta {{#ask:[[Category:Publications]] [[Additional label::FAT4BRAIN]]

...els of oxygen consumption. Hence the best quantitative estimate of ''Rox'' has to be carefully evaluated. Mitochondrial respiration is frequently correcte {{#ask:[[Additional label::Residual oxygen consumption]]

...tegory:Publications]] [[Instrument and method::O2k-Protocol]] [[Additional label::SUIT-001]] |?Has title=Reference

...her form of the ideal gas law (Eq. c). Prior to 1982 the standard pressure has been defined as 101.325 kPa ([[STPD]]). Inserting this standard pressure, s :::: A distinction has to be made between solubility of an ideal gas in the gas phase, ''S''<sub>G

{{#ask:[[Category:Publications]] [[Additional label::SUIT-009 AmR mt D021]] [[Instrument and method::O2k-Protocol]] | ?Has title=Reference

::::* [[Boros Mihaly]]: The package has arrived, thank you so much for the wonderful Easter gift! There is an incre {{#ask:[[Category:Publications]] [[Additional label::MitoEAGLE]]

...the [[count]] ''N''_''X''. The elementary ''U''_''X'' has the same unit [x] as the count ''N''_''X'', or more accurately it ...dividual thing and the unit of anything — be realized? As Carl Gustav Jung has pointed out, a guiding principle of the early constructive alchemists was t

...tegory:Publications]] [[Instrument and method::O2k-Protocol]] [[Additional label::SUIT-002]] |?Has title=Reference

...tate induced upon addition of uncoupler, the required concentration of Omy has to be assessed by the [https://bioblast.at/index.php/Oligomycin Omy titrat ...tegory:Publications]] [[Instrument and method::O2k-Protocol]] [[Additional label::SUIT-006]]

...e 2 ([[ROX]]), yet with reference to Chance and Williams (1956), 'State 2' has later been used for describing this functionally different LEAK state: {{#ask:[[Additional label::LEAK respiration]]

...ular compartments predominantly in the nucleus and mitochondria. Melatonin has pleiotropic functions with powerful antioxidant, anti-inflammatory and onco ...ts, algae, invertebrate and vertebrate organisms. Whereas pineal melatonin has been related with chronobiotic functions, extrapineal melatonin shows mainl

{{#ask:[[Additional label::Count]] |?Has title=Reference

::::: 4 - Switch to 'Y2: O2 slope neg.' by clicking on the Y2 label on the graph legend. ::::: 2 - Switch to plot 'Y1: O2 concentration' by clicking on the Y1 label on the graph legend.

...bers with a volume less than 0.5 to 1 cm³, increasing attention has to be paid to problems of oxygen diffusion and bacterial growth" [2]. ...consumption of tissue homogenates or tissue slices. The Warburg apparatus has its name from the German biochemist Otto Heinrich Warburg (1883-1970) who w

|?Has title=Reference |?Has title=Reference

:::* Labels: indicates that publication has been entered but not yet labelled or that labelling might need revision. ::::* MitoFitPublication: To label publications affiliated with the MitoFit project.

*The European Commission has made a significant contribution to the Coronavirus Global Response pledge w ::::* 2020-03-21 '''WHO EURO has released a guide on how to stay physically active during self-quarantine. I

:::::# Duration and temperature during transport and storage of whole blood has to be monitored carefully and evaluated in terms of final outcome. {{#ask:[[Additional label::MitoEAGLE blood cells reviews]]

...MitoPathways]] and many previous publications, '''CI-linked respiration''' has been used to indicate respiration supported by '''NADH-generating substrate ...low from a combination of NADH-generating substrates (N) and succinate (S) has been indicated as '''CI<small>&</small>II-linked respiration''' ([[Gnaiger

{{#ask:[[Additional label::Living cells]] |?Has title=Reference

{{#ask:[[Additional label::MitoPedia:BME]] |?Has abbr=Abbreviation

{{#ask:[[additional label::IOC32]] |?has title

...opometric indexes. The body adiposity index (Bergman et al 2011), however, has been criticized as insufficiently accurate for clinical applications (Cerqu ...e of height, ''H'', and corresponding reference body mass, ''M''°. The HRP has a body mass excess of zero, BME=0. By extrapolation of the relation between

{{#ask:[[Additional label::MitoFit 2021 BCA]] |?Has title=Reference

:::: The unique '''software DatLab''' has been developed for data acquisition and analysis. DatLab allows the researc {{#ask:[[Category:Publications]] [[Additional label::NextGen-O2k]]

...e biologically induced changes in pH, the buffering capacity of the medium has to be small. This may be addressed either by using or preparing media with ...'''Conditioning:''' Optimum response time will be obtained after the probe has been exposed to two buffer solutions. Place a pH 4 buffer or equivalent in

:::: The project has a total funding of around 450,000 euros and will endorse mobility between n {{#ask:[[Category:Publications]] [[Additional label::mtFOIE GRAS]]

...knowledge that cancer is not only a genetic, but also a metabolic disease, has led scientists to investigate the intricate metabolic plasticity that trans {{#ask:[[Category:Publications]] [[Additional label::TRANSMIT]]

...give the symbol 8 an interpretation and ask for its meaning. The numeral 8 has to be distinguished from it's interpretation as the number that the numeral ...a numerical system of dice, it is a no-dot or even a no-die, since no die has seven sides. The dots yield iconic symbols or figures as counting-numerals,

...al and oesophageal cancers. The number of oral cancers diagnosed in the EU has increased by over 75% in the last 30 years, with long-term survival rates o {{#ask:[[Category:Publications]] [[Additional label::TRACT]]

{{#ask:[[Category:Publications]] [[Additional label::HRP]] |?Has title=Reference

:::: The experimental chamber has an operation volume ''V'' [mL] in its assembled configuration where the sto ...The volume of respiration medium is reduced by ''V''_out (which has a negative value when medium is removed from the chamber) in the open chamb

...Even if ADP concentrations in muscle tissues never reach 5 mM in vivo, one has to increase the ADP concentration to such high or even higher values in pfi ...for respiratory studies with pfi. Unfortunately, oxygen limitation of pfi has been ignored by several research groups ([[Kuznetsov 2008 Nat Protoc|Kuznet

{{#ask:[[Additional label::BME]] |?Has title=Reference

::::::::* '''Oroboros Instruments has signed [[DORA]]'''. ...milar work. The International Committee of Medical Journal Editors (ICMJE) has updated their recommendations to clarify that '''[[preprint]]s''' are not c

...plier is called UltroxRed in the case of Amplex^® UltraRed which has a similar structure to resorufin). The change of emitted fluorescence inten ...serted though the windows of the O2k-Chambers (Hickey et al 2012). The O2k has also been coupled to a fluorescence spectrophotometer with a light guide in

...(''1'') In contrast to physics and mathematics, where publication in arXiv has become a standard, preprints remain relatively unpopular in biomedical scie ...een prompted by post-publication review, where the readership of a journal has detected serious flaws in the published research. .. Manuscripts that make

...is performed by application of mechanical pressure. The digital revolution has transformed the culture of scientific publication in physics and mathematic ...communication of value requires realization in the sense of the word that has more than two [[sides]]: (''1'') a physical realization of the communicatio

:::: The money will be returned AFTER the Grantee has completed her/his visit and submitted a report (a written report on the act ...the 1st day of the STSM. The remaining 50% will be paid after the Grantee has completed her/his visit and submitted a report.

:::: Diagnosis of mitochondrial function has advanced to a new technological level by the introduction of high-resolutio ...metabolic dysregulation and preventable diseases in modern societies, and has an impact on ageing populations in particular. It is becoming increasingly

:::: But the pudding seen as a serving on a dish has been partitioned from a larger mass of pudding. The servings are partitione ...was no counting before counting begins (ESAC), the single individual thing has not yet been counted — ''U''_''X'' is entirely different from a

Training in mitochondrial physiology and bioenergetics, therefore, has high relevance to society. The ‘Blue Book’ on Mitochondrial Pathways an ...rgent pathway architecture of the electron transfer ''system'' (ETS). This has direct implications on the design of substrate-uncoupler-inhibitor titratio

...zing the terminology concerning mitochondrial respiratory states and rates has become increasingly apparent. The chemiosmotic theory establishes the mecha ...t MitoFit funded by the Tyrolian Government, and project NextGen-O2k which has received funding from the European Union’s Horizon 2020 research and inno