Went 2021 MitoFit PB

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Went 2021 MitoFit PB

Publications in the MiPMap
Went N, Di Marcello M, Gnaiger E (2021) Oxygen dependence of photosynthesis and light-enhanced dark respiration studied by High-Resolution PhotoRespirometry. MitoFit Preprints 2021.5. doi:10.26124/mitofit:2021-0005

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MitoFit pdf

Oxygen dependence of photosynthesis and light-enhanced dark respiration studied by High-Resolution PhotoRespirometry

Went Nora, Di Marcello Marco, Gnaiger Erich (2021-04-30) MitoFit Prep

Abstract: The bioenergetic crosstalk between mitochondria and chloroplasts plays a key role in maintaining metabolic integrity and controlling metabolite production for growth and regulation of cell concentration. Dark respiration and photosynthesis were measured in the green alga Chlamydomonas reinhardtii at varying oxygen concentrations and three cell concentrations using the NextGen-O2k with the PhotoBiology Module. Maximum net photosynthesis at a light intensity of 350 µmol∙s-1∙m-2 (blue light) was inhibited at hyperoxia by 40 % at oxygen concentrations of 550 to 650 µM. Light-enhanced dark respiration reached a (negative) maximum within 30 to 60 s after light-dark transitions and was 3.5- to 4-fold higher than steady-state dark respiration independent of O2 concentration in the range of 200 to 650 µM.

Keywords: high-resolution respirometry, photosynthesis, dark respiration, Chlamydomonas reinhardtii Bioblast editor: Gnaiger E O2k-Network Lab: AT Innsbruck Oroboros



link:https://youtu.be/YdlSqOEwi5Y • Podcast

Went 2021 MitoFit Prep Poster.jpg

References

LinkReferenceViewYear
Doerrier C, Garcia-Souza LF, Krumschnabel G, Wohlfarter Y, Mészáros AT, Gnaiger E (2018) High-Resolution FluoRespirometry and OXPHOS protocols for human cells, permeabilized fibers from small biopsies of muscle, and isolated mitochondria. Methods Mol Biol 1782:31-70. doi: 10.1007/978-1-4939-7831-1_3PMID: 29850993 »O2k-brief
O2k-Protocols
2018
Gnaiger E (2020) Mitochondrial pathways and respiratory control. An introduction to OXPHOS analysis. 5th ed. Bioenerg Commun 2020.2:112 pp. https://doi.org/10.26124/bec:2020-0002Bioblast pdfPublished online: 2020-Dec-30

2020
Gnaiger Erich et al ― MitoEAGLE Task Group (2020) Mitochondrial physiology. Bioenerg Commun 2020.1:1-44. https://doi.org/10.26124/bec:2020-0001.v1BEC 2020.1 pdfPublished online: 2020-May-20

2020
Huete-Ortega M, Di Marcello M, Iglesias-Gonzalez J, Gnaiger E (2020) High-resolution respirometry for chloroplast and mitochondrial bioenergetics in Chlamydomonas reinhardtii ― towards biotechnology exploitations. MitoFit Preprint Arch doi:10.26124/mitofit:ea20.algaeurope.0001.
MitoFit pdf
High-resolution respirometry for chloroplast and mitochondrial bioenergetics in Chlamydomonas reinhardtii ― towards biotechnology exploitations
2020-11-30
Shimakawa G, Kohara A, Miyake C (2020) Characterization of light-enhanced respiration in cyanobacteria. Int J Mol Sci 22:342. doi: 10.3390/ijms22010342.PMID: 33396191 Open Access2020
Xue X, Gauthier DA, Turpin DH, Weger HG (1996) Interactions between photosynthesis and respiration in the green alga Chlamydomonas reinhardtii. Characterization of light-enhanced dark respiration. Plant Physiol 112:1005–14. https://doi.org/10.1104/pp.112.3.1005Open Access1996

Event

» Presented at ISAP2021

Support

Template NextGen-O2k.jpg
Supported by project NextGen-O2k which has received funding from the European Union’s Horizon 2020 research and innovation programme under grant agreement No. 859770.


Labels: MiParea: Respiration, Instruments;methods, Comparative MiP;environmental MiP 


Organism: Plants 

Preparation: Intact cells 

Regulation: Oxygen kinetics  Coupling state: ROUTINE 

HRR: Oxygraph-2k, NextGen-O2k 

Algae, LEDR, Photosynthesis