O2k-Demo experiments

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O2k-Demo experiments

O2k-Procedures
SUIT-Protocol

Oroboros O2k demo

ProtocolViewTopicPreparationTissue and cellMammal and modelLast update
Gnaiger 2020 MitoPathwaysBioblast pdf 5th edition in preparationGnaiger E (2020) Mitochondrial pathways and respiratory control. An introduction to OXPHOS analysis. 5th ed. Bioenerg Commun 2020.2 (in prep).Intact cells
Permeabilized cells
Permeabilized tissue
Homogenate
Isolated mitochondria
Heart
Skeletal muscle
Fibroblast
Human
Mouse
2020
MiPNet12.01 Suppl T-issueBioblast pdf »Versions
O2k-Protocols contents
MitoPathways at the Q-junction: mouse skeletal muscle fibers.
Permeabilized tissueSkeletal muscleMouse2018-11-18
MiPNet08.09 CellRespirationBioblast pdf  »Versions
O2k-Procedures contents
High-resolution respirometry and coupling-control protocol with living cells: ROUTINE, LEAK, ET-pathway, ROX.
Intact cellsBlood cells
Lymphocyte
Human2016-08-11
MiPNet17.03 Shredder vs FibersBioblast pdf »Versions
O2k-Protocols
Mitochondrial respiration in permeabilized fibers versus homogenate from fish liver and heart. An application study with the PBI-Shredder.
HomogenateHeart
Liver
Fishes2015-06-18
MiPNet12.11 MitoRespirationBioblast pdf »Versions
O2k-Protocols
Flux control ratios in isolated mitochondria. OXPHOS capacity and respiratory control.
Isolated mitochondriaArtemia
Crustaceans
2014-02-20
Gnaiger 2014 MitoPathwaysBioblast pdf
O2k-Protocols
Gnaiger E (2014) Mitochondrial pathways and respiratory control. An introduction to OXPHOS analysis. 4th ed. Mitochondr Physiol Network 19.12. Oroboros MiPNet Publications, Innsbruck:80 pp.
Permeabilized cells
Permeabilized tissue
Homogenate
Isolated mitochondria
Intact cells
Heart
Skeletal muscle
Fibroblast
Human
Mouse
2014
Pesta 2012 Methods Mol BiolBioblast pdf
PMID: 22057559
O2k-Protocols
Pesta D, Gnaiger E (2012) High-resolution respirometry. OXPHOS protocols for human cells and permeabilized fibers from small biopsies of human muscle. Methods Mol Biol 810:25-58.
Permeabilized cells
Permeabilized tissue
Intact cells
Skeletal muscle
Other cell lines
HEK
Fibroblast
HUVEC
Human2012
Gnaiger 2008 POSBioblast pdf
O2k-Protocols contents
Gnaiger E (2008) Polarographic oxygen sensors, the oxygraph and high-resolution respirometry to assess mitochondrial function. In: Mitochondrial Dysfunction in Drug-Induced Toxicity (Dykens JA, Will Y, eds) John Wiley & Sons, Inc, Hoboken, NJ:327-52.
Permeabilized cells
Permeabilized tissue
Intact cells
Blood cellsMouse2008
TIP2k-Module

O2k-Demo TIP2k

 » O2k-Catalogue: TIP2k
 » O2k-Publications: TIP2k
ProtocolViewTopicPreparationTissue and cellMammal and modelLast update
Pesta 2012 Methods Mol BiolBioblast pdf
PMID: 22057559
O2k-Protocols
Pesta D, Gnaiger E (2012) High-resolution respirometry. OXPHOS protocols for human cells and permeabilized fibers from small biopsies of human muscle. Methods Mol Biol 810:25-58.
Permeabilized cells
Permeabilized tissue
Intact cells
Skeletal muscle
Other cell lines
HEK
Fibroblast
HUVEC
Human2012
Gnaiger 2008 POSBioblast pdf
O2k-Protocols contents
Gnaiger E (2008) Polarographic oxygen sensors, the oxygraph and high-resolution respirometry to assess mitochondrial function. In: Mitochondrial Dysfunction in Drug-Induced Toxicity (Dykens JA, Will Y, eds) John Wiley & Sons, Inc, Hoboken, NJ:327-52.
Permeabilized cells
Permeabilized tissue
Intact cells
Blood cellsMouse2008
MiPNet04.05 Titration-InjectionBioblast pdf
O2k-Protocols contents
From step titration to ramp injection: Uncoupling by FCCP with TIP.
Intact cellsEndothelial;epithelial;mesothelial cell
HUVEC
Human1999-01

TIP2k Manual

ChapterViewSectionLast update
MiPNet12.10 TIP2k-manualBioblast pdf »Versions
O2k-Manual
Titration-Injection microPump. TIP2k user manual.
2016-08-10



O2k-FluoRespirometer
link=O2k-TPP%2B_and_Ca2%2B_ISE-Module

O2k-Demo O2k-MultiSensor

 » O2k-Catalogue: O2k-MultiSensor
 » O2k-Publications: O2k-Fluorometry
ProtocolViewTopicPreparationTissue and cellMammal and modelLast update
MiPNet24.11 mtMP calculationPDF.jpg » Versions
O2k-Protocols
Mitochondrial membrane potential calculation
2019-08-05
MiPNet18.05 Amplex-Mouse-heartBioblast pdf »Versions
O2k-Protocols
O2k-Fluorometry: HRR and H2O2 production in mouse cardiac tissue homogenate.
HomogenateHeartMouse2019-06-24
MiPNet20.14 AmplexRed H2O2-productionBioblast pdf »Versions
O2k-Protocols
O2k-FluoRespirometry: HRR and simultaneous determination of H2O2 production with Amplex UltraRed.
Isolated mitochondriaHeartMouse2019-06-24
MiPNet20.13 Safranin mt-membranepotentialBioblast pdf »Versions
O2k-Protocols
O2k-FluoRespirometry: HRR and simultaneous determination of mt-membrane potential with safranin or TMRM.
HomogenateNervous systemMouse2019-06-24
Krumschnabel 2015 Methods Mol BiolPMID: 25631019
O2k-Protocols contents
Krumschnabel G, Fontana-Ayoub M, Sumbalova Z, Heidler J, Gauper K, Fasching M, Gnaiger E (2015) Simultaneous high-resolution measurement of mitochondrial respiration and hydrogen peroxide production. Methods Mol Biol 1264:245-61.
Isolated mitochondriaNervous systemMouse2015
Makrecka-Kuka 2015 BiomoleculesPMID: 26131977 Open Access
O2k-Protocols contents
Makrecka-Kuka M, Krumschnabel G, Gnaiger E (2015) High-resolution respirometry for simultaneous measurement of oxygen and hydrogen peroxide fluxes in permeabilized cells, tissue homogenate and isolated mitochondria. Biomolecules 5:1319-38.
Permeabilized cells
Homogenate
Isolated mitochondria
Intact cells
Heart
Nervous system
HEK
Human
Mouse
2015
Krumschnabel 2014 Methods EnzymolPMID: 24862266 »O2k-brief
O2k-Protocols contents
Krumschnabel G, Eigentler A, Fasching M, Gnaiger E (2014) Use of safranin for the assessment of mitochondrial membrane potential by high-resolution respirometry and fluorometry. Methods Enzymol 542:163-81.
HomogenateNervous systemMouse2014
MiPNet08.12 IOC22Bioblast pdf
O2k-Protocols
NO effect on mitochondrial oxygen kinetics at low oxygen. O2k workshop Report.
Isolated mitochondriaLiverRat2011-12-11

O2k-MultiSensor Manual

ChapterViewSectionLast update
MiPNet15.03 O2k-MultiSensor-ISEBioblast pdf »Versions
O2k-Manual
O2k-MultiSensor system with ion selective electrodes (ISE).
2018-01-25
MiPNet15.05 NO-manualBioblast pdf  »Versions
O2k-Manual
O2k-MultiSensor system with amperometric sensors: NO, H2S, H2O2.
2016-04-21
MiPNet17.05 O2k-Fluo LED2-ModuleBioblast pdf »Versions
O2k-Manual
O2k-Fluo LED2-Module.
2016-08-09
MiPNet23.15 O2k-pH ISE-Module
O2k-Manual
Measurement of proton flux with the O2k-pH ISE-Module.
2018-09-08


O2k-Guides: »O2k-Catalogue :: »O2k-Manual :: »O2k-Publications

O2k-Procedures » Link
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» O2k-Procedures: MitoPathways
SUIT-Protocol
» O2k-Demo experiments
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» O2k-chemicals and media
PBI-Shredder HRR-Set.JPG
» O2k-mitochondrial preparations
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» O2k-Procedures: Mitochondrial and marker-enzymes