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OroboPedia topics

TermDescription
10th European Algae Industry Summit 2020 Reykjavik IS
10th Int CeBiTec Research Conference 2021 Bielefeld DE
12th ÖGMBT Annual Meeting 2020 Virtual Event
16th Chinese Biophysics Congress 2018 Chengdu CH
19th Beijing Conference and Exhibition on Instrumental Analysis 2021 Beijing CN
19th Chinese Biophysics congress 2021 Anhui CN
1st Myocardial Function Symposium 2020 Graz AT
2020 PaduaMuscleDays Padua IT
24th Kalorimetrietage 2021 Braunschweig DE
46th ISOBM Congress 2019 Athens GR
4th China Symposium on Nerve Control Metabolism 2021 Hangzhou city CN
4th edition Metabolism & Cancer 2021 Virtual
6th Biannual Meeting on Mitochondria Apoptosis & Cancer 2019 Prague CZ
7th World Congress on Targeting Microbiota 2019 Krakow PL
8th SMRM and Mitochondria-Metabolism Network Meeting 2020 Pune IN
ALAS
ALGAEUROPE 2018 Amsterdam NL
AR Buenos Aires Boveris A
ASMRM & J-mit 2019 Fukuoka JP
ASMRM 2017 Xian CN
ASMRM 2018 Busan KR
ASMRM 2021 Singapore SG
AT Graz Graier W
AT Graz Zechner R
AT Innsbruck Burgstaller W
AT Innsbruck Burtscher M
AT Innsbruck Gnaiger E
AT Innsbruck Inncode technologies
AT Innsbruck Jansen-Duerr P
AT Innsbruck MitoFit
AT Innsbruck Oroboros
AT Innsbruck Schneeberger S
AT Kolsass WGT
AT Salzburg Breitenbach M
AT Salzburg Sperl W
AT Vienna Bittner RE
AT Vienna Kozlov AV
AU Chermside Molenaar P
AU Clayton St John J
AU Melbourne Bishop DJ
AU Melbourne Bond S
AU Melbourne Coughlan M
AU Melbourne Hardee JP
AU Melbourne Thouas G
AU Melbourne Trounce IA
AU Melbourne White C
AU Perth Filipovska A
AU Queensland Neuzil J
AU Southport Peart J
AU St Lucia Coombes J
AU Sydney Ballard JW
AU Sydney Griffith SC
AU Sydney James D
AU Sydney Kong S
AU Sydney Philp A
AU Sydney Stocker R
Aasander Frostner 2022 MitoFit
Abbasi 2018 J R Soc Med
Abed Rabbo 2022 MitoFit
Add Graph/Delete bottom graphAdd: A new graph is added at the bottom of the screen. Select plots for display in the new graph, Ctrl+F6. Delete: Delete one of the graphs displayed in DatLab.
Alencar 2022 MitoFit
AlgaEurope 2020 Virtual Event
AlgaEurope 2022 Rome IT
All O2k-Workshop
Allahverdiyeva-Rinne Yagut
Amp calibration - DatLabAmp calibration indicates the calibration of the amperometric O2k-channel.
Amperometric,AmpAfter selection of the Amperometric, Amp channel in the O2k configuration, an Amperometric, Amp tab will appear in the O2k control [F7] window. Set the desired light intensity (0-1600) in the field ´Fluo intensity´ and the desired amplification of the signal (1-1000) in the field ´Gain for Fluo sensor´in the Amperometric, Amp window followed by a left-click Send to O2k. Switching off the illumination before each fluorometric measurement is routinely required.
Analytica China 2020 Shanghai CN
Attached cellsMany cell types are grown in culture as attached cells, such as endothelial or neuronal cells in a monolayer.
Automatic pan - DatLabAutomatic pan (only for real-time data recording) toggles automatic panning on/off by clicking in the O2k status line. If it is on (green), the time range is maintained while the time axis always shows the currently recorded data, i.e. the value of the offset (minimum value) increases as experimental time proceeds. If it is off (yellow), the time axis is static. This allows for manually panning backwards to observe previous sections of the experiment at a given time range. In this mode, the actual experimental time may be off-scale. Toggle between "Pan auto" and "Pan off" by a left-click on the text. It does not influence continuous data recording. It is recommended to maintain automatic panning on during the experiment, except for specifically viewing earlier sections of the experiment.
AutoscaleAutoscale zooms in or out of the selected period with Autoscale time axis, Autoscale Y1 (Y2) axes and Automatic pan.
Autoscale Y1 (Y2) axesAutoscale Y1 (Y2) axes: Autoscaling the measured values (full data range) on the Y1 (Y2) axis in the selected plot.
Autoscale time axisAutoscale time axis gives an overview of the entire experimental period.
Avasthi 2018 eLife
BE Gent Braeckman BP
BE Leuven Spinazzi M
BE Leuven Vermeersch P
BE Liege Sluse F
BE Liege Votion DM
BEC 2020.1 doi10.26124bec2020-0001.v1
BPS19 2019 Baltimore US
BPS2023 San Diego US
BR Brasilia De Bem AF
BR Campinas Carneiro EM
BR Campinas Castilho R
BR Campinas Vercesi AE
BR Criciuma Dal-Pizzol F
BR Criciuma Muller AP
BR Florianapolis Latini A
BR Jaboticabal Oliveira MT
BR Manaus Val AL
BR Porto Alegre Klamt F
BR Porto Alegre Souza DOG
BR Ribeirao Preto Alberici LC
BR Rio de Janeiro Da Poian AT
BR Rio de Janeiro Galina A
BR Rio de Janeiro Institute Biomedical Chemistry
BR Rio de Janeiro Moura AS
BR Rio de Janeiro Oliveira MF
BR Rio de Janeiro Paes MC
BR Rio de Janeiro Rumjanek FD
BR Rio de Janeiro Vieyra A
BR Santa Maria Soares FA
BR Sao Gabriel Franco JL
BR Sao Paulo Bresciani Martins de Andrade P
BR Sao Paulo Ferreira JCB
BR Sao Paulo Festuccia W
BR Sao Paulo Inada NM
BR Sao Paulo Kowaltowski AJ
BR Sao Paulo Silber AM
Baglivo 2022 MitoFit-QC
Balbaisi 2022 MitoFit
Beno Marija
Berg 2016 Science
Berg 2017 Science
Bhalla 2016 Mol Biol Cell
Bioblast 2012
Bioblast 2012: Registration and accommodation
Bioblast 2012: Scientific Committee
Bioblast 2022
Bioblast 2022 - Organisation
Bioblast 2022 - Participants
Bioblast 2022 - Program overview
Bioblast:About
Biological contaminationBiological contamination may be caused by microbial growth in the O2k-Chamber or in the experimental medium.
Block temperatureThe block temperature of the Oroboros O2k is the continuously measured temperature of the copper block, housing the two glass chambers of the O2k. The block temperature is recorded by DatLab as one of the O2k system channels.
Bolivia-Mount Chacaltaya 2012
Bombaca 2022 MitoFit
Bourne 2017 PLoS Comput Biol
Bove-Fenderson 2018 JBMR Plus
Brown 2020 MitoFit Preprint Arch
CA Antigonish Kane DA
CA Calgary Kinesiology Univ Calgary
CA Calgary Shearer J
CA Chalk River Paterson L
CA Charlottetown Kamunde C
CA Edmonton Lemieux H
CA Edmonton Seubert J
CA Edmonton Zaugg M
CA Guelph Ballantyne JS
CA Guelph Holloway GP
CA Hamilton Scott GR
CA Hamilton Singh G
CA Hamilton Tarnopolsky MA
CA Kelowna Islam H
CA Kingston McGlory C
CA London Staples JF
CA Moncton Boudreau L
CA Moncton Hebert-Chatelain E
CA Moncton Pichaud N
CA Montreal Bergdahl A
CA Montreal Breton S
CA Montreal Gouspillou G
CA Montreal Hepple RT
CA Ottawa Darveau CA
CA Ottawa Harper ME
CA Ottawa Pamenter M
CA Quebec Soliz J
CA Rimouski Blier PU
CA Saint John Pulinilkunnil T
CA Sherbrooke Blondin DP
CA Toronto Hood DA
CA Toronto Perry CG
CA Vancouver Boushel RC
CA Vancouver Richards JG
CA Waterloo Joseph JW
CA Waterloo McDonald AE
CA Waterloo Quadrilatero J
CA Winnipeg Banerji V
CA Winnipeg Fernyhough P
CA Winnipeg Treberg JR
CH Basel Balavenkatraman KK
CH Basel Eckert A
CH Basel Kraehenbuehl S
CH Basel Uteng M
CH Bern Djafarzadeh S
CH Bern Longnus SL
CH Bern Nuoffer JM
CH Lausanne Auwerx J
CH Lausanne Bagni C
CH Lausanne Canto C
CH Lausanne EPFL
CH Lausanne Place N
CH Lausanne Sandi C
CH Zurich Gassmann M
CH Zurich Lundby C
CH Zurich University of Zurich Physiology
CH Zurich Wallimann T
CL Santiago Regueira T
CN Baoding Cao X
CN Baoding Liu F
CN Beijing Chen Q
CN Beijing Huawei
CN Beijing Li C
CN Beijing Liu L
CN Beijing Qin Y
CN Beijing Wang X
CN Beijing Yuan Z
CN Chongqing Huang J
CN Chongqing Zhu Z
CN Dalian Zhan L
CN Guangzhou Liu J
CN Guangzhou Zhang X
CN Hangzhou Zhu W
CN Hongkong Huawei
CN Jinan Wang C
CN Macao Huawei
CN Nanjing Gan Z
CN Shanghai Liu T
CN Shanghai Yi X
CN Shanghai Zenda
CN Tianjin Zhang Y
CN Tianjin Zhao H
CN Wuhan Huang K
CN Xiamen Lin SC
CN Zengzhou Wu S
CN Zunyi Zhou S
CO Medellin Gomez LA
CSH Asia 2017 Suzhou CN
CSLMB 2018 Shanghai CN
CU Havana Pardo Andreu GL
CZ Ceske Budejovice Zikova A
CZ Hradec Kralove Cervinkova Z
CZ Olomouc Modriansky M
CZ Pardubice Rousar T
CZ Pilsen Kuncova J
CZ Prague Bioenergetics
CZ Prague Dvorak A
CZ Prague Fisar Z
CZ Prague Houstek J
CZ Prague Jezek P
CZ Prague Kalous M
CZ Prague Kopecky J
CZ Prague Krajcova A
CZ Prague Neuzil J
CZ Prague Pichova A
CZ Prague Zeman J
CalciumCa2+ is a major signaling molecule in both prokaryotes and eukaryotes. Its cytoplasmic concentration is tightly regulated by transporters in the plasma membrane and in the membranes of various organelles. For this purpose, it is either extruded from the cell through exchangers and pumps or stored in organelles such as the endoplasmic reticulum and the mitochondria. Changes in the concentration of the cation regulate numerous enzymes including many involved in ATP utilizing and in ATP generating pathways and thus ultimately control metabolic activity of mitochondria and of the entire cell. Measuring changes in Ca2+ levels is thus of considerable interest in the context of high-resolution respirometry.
Calcium GreenCalcium GreenTM (CaG) denotes a family of extrinsic fluorophores applied for measurement of Ca2+ concentration with mitochondrial preparations. This dye fluoresces when bound to Ca2+. When measuring mitochondrial calcium uptake it is possible to observe the increase of the CaG signal upon calcium titration, followed by the decrease of CaG signal due to the uptake.
Callaway 2013 Nature
Canonical Carol Canon O
Cardoso 2021 MitoFit MgG
Cardoso 2022 MitoFit rTCA
Cardoso Luiza HD
Career Event - FH Campus 2021 Wien AT
Cecatto 2022 MitoFit CaG
Chabi 2019 MitoFit Preprint Arch EA
Chalmers 2016 F1000Research
Channel» See O2k signals and output
Chawla 2017 Nature
Chicco 2022 MitoFit
Chinese Academy of pathophysiology, endocrinology and metabolism Specialized Committee Conference 2018 Tianjin CN
Chinopoulos Christos
Chlamy 2021 Ile des Embiez FR
ChlororespirationIn chlororespiration oxygen is consumed by a putative respiratory electron transfer system (ETS) within the thylakoid membrane of the chloroplasts and ATP is produced. It is a process that involves the interaction with the photosynthetic ETS in which NAD(P)H dehydrogenase transfers electrons to oxygen with the assistance of the photosynthetic plastoquinone (PQ), which acts as a non-photochemical redox carrier. Initially described in the unicellular alga Chlamydomonas reindhartdii, chlororespiration was highly disputed for years until the discovery of a NAD(P)H-dehydrogenase (NDH) complex (plastidic encoded) and plastid terminal oxidase (PTOX) (nuclear encoded) in higher-plant chloroplasts. PTOX is homologous to the plant mitochondrial alternative oxidase and has the role of preventing the over-reduction of the PQ pool while the NDH complexes provide a gateway for the electrons to form the ETS and consume oxygen. As a result of this process there is a cyclic electron flow around Photosystem I (PSI) that is activated under stress conditions acting as a photoprotection mechanism and could be involved in protecting against oxidative stress.
Citron 2015 Proc Natl Acad Sci U S A
Close - DatLabClose a DatLab file.
Closed chamberThe O2k-chamber can be used as a closed system or open system. Gas bubbles must be avoided.
Cobb 2017 PeerJ Preprints
Committee 2018 COPE Discussion Document
Connect to O2kConnect to O2k connects DatLab with the O2k. Select the USB port (or Serial port) with the corresponding cable connecting your PC to the O2k. Select the subdirectory for saving the DLD file. Then data recording starts with experimental time set at zero.
Connection windowAfter starting DatLab either the Connection window opens automatically by default or open O2k control by pressing [F7] and select the communication port.
Copy marksIn Copy marks, Marks in DatLab are copied from a seleted Plot to the active plot.
Copy marks - DatLab
Copy to clipboardIn DatLab Copy to clipboard can be used to copy selected graphs or values and to paste them to your preferred program or file (e.g. Word, Excel).
Cover-Slip\black
Cover-Slip black.JPG
A Cover-Slip should be placed on top of the O2k-Stopper to minimize contamination and evaporation of liquid extruding from the capillary of the stopper. The Cover-Slips do not exert any direct effect on oxygen backdiffusion into the O2k-chamber. Use the the Cover-Slip\black to avoid light penetration and disturbance of fluorescence signals and generally for optical measurements in the O2k.
Credit card payment
Crispim 2019 MitoFit Preprint Arch EA
CureMILS
Custom labelA Custom label can be entered in this box to rename the axis label. Two lines are available for the axis name and unit.
Custom-made stoppersStoppers can be custom-made for applications with user-specific sensors according to customer specifications.
Cyclic voltammetryCyclic voltammetry (CV) is a type of electrochemical measurement which is applied with the Q-Module as quality control to

(1) determine the oxidation and reduction peak potentials of Coenzyme Q in the specific experimental condition, (2) check the quality of the Q-Sensor, and (3) test the interference of chemicals used in the HRR assay with the Q-Sensor. In CV, the Q-Sensor with the three-electrode system is used to obtain information about the analyte (CoQ) by measuring the current (I) as the electric potential (V) between two of the electrodes is varied. In CV the electric potential between the glassy carbon (GC) and the Ag/AgCl reference electrode changes linearly versus time in cyclical phases, while the current is detected between GC and platinum electrode (Pt). The detected current is plotted versus the applied voltage to obtain the typical cyclic voltammogram trace (Figure 1). The presence of substances that are oxidized/reduced will result in current between GC and Pt, which can be seen as characteristic peaks in the voltammogram at a defined potential. The oxidation or the reduction peak potential values are used to set the GC (integrated into the Q-Sensor) for a separate experiment to measure the Q redox state of a biological sample. The oxidation and reduction peak potentials can be influenced by 1) the respiration medium, 2) the type of CoQ, 3) the polarization window, 4) the scan speed, 5) the number of cycles, 6) the concentration of the analyte (CoQ), and 7) the initial polarization voltage. <be>

-See: MiPNet24.12 NextGen-O2k: Q-Module.
MiPNet24.16 DatLab8.0: CV-Module
Cyclic voltammetry - DatLabCyclic voltammetry
DE Berlin Boschmann M
DE Biberach Markgraf D
DE Bonn Kunz WS
DE Bonn Pfeifer A
DE Bremerhaven Mark FC
DE Cologne Aging Research
DE Cologne Antebi A
DE Cologne Larsson NG
DE Cologne Pesta D
DE Cologne Trifunovic A
DE Duesseldorf Grieshaber MK
DE Duesseldorf Haendeler J
DE Duesseldorf IUF
DE Duesseldorf Ibing W
DE Duesseldorf Roden M
DE Duesseldorf Westenfeld R
DE Essen Bienholz A
DE Essen De Groot H
DE Essen Ferenz K
DE Essen Ferenz KB
DE Essen Gedik N
DE Essen Kirsch M
DE Frankfurt Droese S
DE Frankfurt Eckert GP
DE Frankfurt Leeuw T
DE Frankfurt Osiewacz HD
DE Frankfurt Sanofi
DE Frankfurt Schmoll D
DE Frankfurt Wittig I
DE Freiburg Pfanner N
DE Freiburg Schuele R
DE Freising Klingenspor M
DE Giessen Eckert GP
DE Giessen Rohrbach S
DE Giessen Weissmann N
DE Goettingen Dennerlein S
DE Goettingen Krischek C
DE Goettingen Moerlein D
DE Goettingen Mueller M
DE Goettingen Wicke M
DE Hamburg Heine M
DE Hamburg Singer D
DE Hannover Hildebrandt T
DE Heidelberg Labeit S
DE Heidelberg Mairbaeurl H
DE Heidelberg Teleman A
DE Hottersheim Kabiri M
DE Jena Szibor M
DE Kiel Herdegen T
DE Koblenz Ortmann C
DE Konstanz Brdiczka D
DE Kronshagen Grams B
DE Leipzig Maskow T
DE Leipzig Mueller A
DE Leipzig UFZ Environmental Research
DE Magdeburg Debska-Vielhaber G
DE Magdeburg Gellerich FN
DE Magdeburg Klinik Neurologie
DE Magdeburg Schild L
DE Magdeburg Schoenfeld P
DE Mainz Methner A
DE Munich Elstner M
DE Munich Jastroch M
DE Munich Perocchi F
DE Munich Wollenberg B
DE Munich Zischka H
DE Nuthetal Klaus S
DE Regensburg Reichold M
DE Regensburg Renner-Sattler K
DE Rostock Sokolova I
DE Seewiesen Casagrande S
DE Tuebingen Weigert C
DE Ulm Gumpp A
DE Ulm Karabatsiakis A
DE Ulm Radermacher P
DE Wuerzburg Maack C
DK Aarhus Boetker HE
DK Aarhus Fago A
DK Aarhus Martensen PM
DK Copenhagen Christiansen M
DK Copenhagen Dela F
DK Copenhagen Gerhart-Hines Z
DK Copenhagen Larsen A
DK Copenhagen Larsen S
DK Copenhagen Lundby C
DK Copenhagen Pilegaard H
DK Copenhagen Quistorff B
DK Malov Hey-Mogensen M
Da Silva 2018 Med J Armed Forces India
Dambrova 2022 MitoFit
DatLab
DatLab
DatLab is the O2k-Software for Data Acquisition & Analysis, specifically developed for high-resolution respirometry with the O2k-FluoRespirometer.

The newest DatLab version is DatLab 7.4, included in the O2k-FluoRespirometer.


The minimum computer requirements are Intel-Core-2 or equivalent CPU, 2GB RAM and Windows XP. However, we recommend Intel i5 or equivalent CPU, 4GB RAM, Windows 10 and SSD. For the proper display of DatLab on your computer, please make sure the “Language settings” are set to English.
DatLab 2DatLab 2 (DL2) is a MS-DOS programe. DL2 is still used for analysis of oxygen kinetics, after exporting files recorded in recent DatLab versions. A user-friendly O2-kinetics module is in preparation (DL8).
DatLab data fileThe file type generated by DatLab is *.DLD.
DatLab error messagesCommon DatLab error messages and according actions and solutions are listed here.
DatLab installation
DatLab
We recommend a 'clean install' for DatLab installation: rename your previous DatLab programme subdirectory (e.g. C:\DatLab_OLD). The standard Instrumental and SUIT DL-Protocols package is automatically implemented with the simple DatLab programme installation.
DatLab oxygen flux: performance and data analysisThe quality of the results are strongly affected by the performance and data analysis. Therefore, we provide guidelines for performing and evaluating respirometric assays.
DatLab templatesDatLab templates can be imported for O2k-setups, graph layouts, mark names, TIP2k setups and marks statistics configurations.
See also » Manage setups and templates
DatLab-Analysis templatesGo in DatLab to Mark statistics (F2), select which type of marks you want to export ("All marks in plot" or "DL-Protocol marks", with 3 possibilities each), then click on [Copy to clipboard] to copy selected values and paste them to a DatLab-Analysis template for numerical and graphical data analysis.
DatLab-Upgrading to DatLab 6DatLab-Upgrading to DatLab 6: including free follow-up updates for DatLab 6 for the next two years
DatLab-Upgrading\4.x-5.2DatLab-Upgrading\4.1-5.2: Upgrading DatLab 4.x to 5.2, incl. O2k-Manual, with free follow-up updates of DatLab 5.2. Discontinued: see higher DatLab version.
Data labels and units - DatLab
Data masks - DatLab
Data recording intervalThe data recording interval is the time interval at which data is sampled with an instrument. In DatLab the data recording interval is set in the O2k control window. The system default value is 2 s. A lower data recording interval is selected for kinetic experiments, and when the volume-specific oxygen flux is high (>300 pmol O2·s-1·ml-1).
Technically, the O2k instrument (hardware) measures the sensor signal every 10ms (which is NOT the „data recording interval“). By the given data recording interval from DatLab (software) a discrete number of sensor signal points are taken to calculate an average value in the O2k (e.g. a data recording interval of 2 s can take 200 sensor signal points; a data recording interval of 0.5 s can take 50 sensor signal points). This average value is sent to DatLab and is recorded as a raw data point. However, there is a defined threshold: the O2k does not apply more than 200 sensor signal points to calculate the average for the raw data point. For example a data recording interval of 3 s could take 300 sensor signal points but only the 200 most recent sensor signal points are used for averaging.
Default labelThe Default label is the system default value for the axis label. These labels are changed automatically, according to the selected channel and unit. To change this label enter a Custom label.
Delete - DatLabDelete a DLD file. The decision to delete a file containing no useful data can be made most easily when viewing the traces. Only available when disconnected from the O2k.
Delete pointsSelect Delete points in the Mark information window to remove all data points in the marked section of the active plot. See also Interpolate points and Restore points or Recalculate slope.
Deselect channelsChannels can be selected/deselected in DatLab in the O2k configuration. Deselect all O2k-MultiSensor channels in O2k-Core applications. Select only the specifically used channels in O2k-MultiSensor applications.
Desjardins-Proulx 2013 PLOS Biol
Development MitoFit proficiency test
Di Marcello 2019 MitoFit Preprint Arch EA
Di Marcello Marco
Die Kraftwerke der Zellen
Different O2 fluxes in left and right chamberWhat are potential causes for different O2 fluxes in the left and right chamber?
Display DatLab helpDisplay DatLab help

In this section, we present some issues that could happen during your data analysis related to the graphs display and how to fix them quickly.

Case in which an issue might occur:

  • While analysing your data, trying to close the program while the graph is still being loaded. If you cancel the closing window, the graph will not be loaded at the screen.

In the event of a frozen display of the graphs, try the alternatives below:

  • Click on: Graph > Autoscale time axis
  • Click on: Graph > Scaling (F6); then press OK to confirm the scaling and induce the program to reload the graphs (no changes in the graphs are required).
Display Power-O2kThe Power-O2k number, which is set in the pull-down menu Oroboros O2k \ O2k configuration, is shown in the active graph. To show it in graphs copied to clipboard, the option "Show Oroboros icon in clipboard files" must be enabled in the Graph-menu Graph options - DatLab.
Display numerical valueIf Display numerical value the current numerical values are displayed in the graph for the active plots on the Y1 axis and Y2 axis (during data acquisition only).
DithioniteDithionite Na2S2O4 is the 'zero oxygen solution powder' used for calibration of oxygen sensors at zero oxygen, or for stepwise reduction of oxygen concentrations in instrumental O2 background tests. It is not recommended to use dithionite in experiments with biological samples or several multisensor approaches, for these see Setting the oxygen concentration.
Doerrier 2019 MitoFit Preprint Arch EA
Doerrier Carolina
Donnelly 2022 MitoFit Hypoxia
EE Tallinn Kaambre T
EE Tallinn Saks VA
EE Tartu Paju K
EE Tartu Seppet EK
EG Cairo Ali SS
ES Barcelona Bosch M
ES Barcelona Claret M
ES Barcelona Garcia-Roves PM
ES Barcelona Gomis R
ES Barcelona IDIBAPS Hospital Clinic
ES Barcelona Moren C
ES Barcelona Zorzano A
ES CN Las Palmas Calbet JAL
ES Granada Acuna-Castroviejo D
ES L'Hospitalet Bermudez MJ
ES Lleida Boada J
ES Madrid Cadenas S
ES Madrid Enriquez JA
ES Madrid Garesse R
ES Malaga Medina MA
ES Pamplona Izquierdo M
ES Santiago De Compostela Mendez-Alvarez E
ES Tarragona Arola L
ES Valencia Casado Pinna M
ES Valencia Gomez Cabrera
ES Valencia Meseguer S
ES Zaragoza Ruiz-Pesini E
ESCI 2021 Virtual
ESP2021 Salzburg AT
Electrolyte\Reference-Electrode
Electrolyte Reference-Electrode.jpg
Electrolyte\Reference-Electrode for Reference-Electrode\2.4 mm
Electronic-TIP2k Upgrading\O2k-Main Unit Series A-DElectronic-TIP2k Upgrading\O2k-Main Unit Series A-D - Former Product : not required for O2k-Core, the O2k-Main Unit has to be returned to the OROBOROS workshop.
Electronic-TIP2k Upgrading\O2k-Main Unit Series EElectronic-TIP2k Upgrading\O2k-Main Unit Series E - Former Series : not required for O2k-Core, free of charge for Series E in conjunction with the purchase of the TIP2k-Module, the O2k-Main Unit has to be returned to the OROBOROS workshop.
Enable DL-Protocol editingEnable DL-Protocol editing is a novel function of DatLab 7.4 offering a new feature in DL-Protocols: flexibility. Fixed sequences of events and marks can be changed (Skip/Added) in a SUIT protocol by the user. Moreover, the text, instructions, concentrations and titration volumes of injections in a specific DL-Protocol can be edited and saved as user-specific DL-Protocol [File]\Export\DL-Protocol User (*.DLPU). To enable it, under the 'Protocols' tab in the menu, select the option 'Enable DL-Protocol editing', and then select the plot in which the marks will be set (e.g., O2 flux per V). Select the 'Overview' window, where you will be able to edit events and marks names, definition/state, final concentration and titration volumes, as well as select a mark as 'multi' for multiple titration steps, skip a mark, or add a new event or mark. After saving, export a DL-Protocol User (DLPU) and load it before running the next experiments. If users of DatLab versions older than DatLab 7.4 wish to alter the nature of the chemicals used or the sequence of injections, we ask them to contact the O2k-Technical Support.

For more information:

PlayVideo.jpg Export DL-Protocol User (*.DLPU)
Events - DatLabAn event in DatLab is a defined point in time, labelled by a name (1 to 10 characters). An event applies to all plots of the selected O2k-Chamber. The event is shown by a vertical line in the graph and the label of the event is shown on the top (DatLab 6 and lower: on the bottom). The default name is the sequential number of the event. It is recommended to edit event labels with a minimum number of characters, and to explain the abbreviation in the 'Definition' box. The final concentration and titration volume can be entered into the corresponding boxes, if the event relates to the titration of a substance. A short comment can be entered to describe the event in detail.

Set events - Manual events are entered (real-time, connected to the O2k) by pressing [F4] at the time of the event (e.g. to indicate a manual titration into the chamber). An event belongs either to chamber A, chamber B, or both. Instrumental events are added automatically, e.g. when the stirrer (A or B) or illumination (both chambers) is switched on or off. After setting a new event the Edit event window pops up. Pressing F4 defines the time point of the event. Full attention can then be paid to the experiment. Edit the event later, as it is possible to insert an event at any chosen moment of the plotted record of the experiment by placing the cursor anywhere in the graph at the selected time point by pressing Ctrl and clicking the left mouse button. Edit event - Left click on the name of an existing event to open the Edit event window to edit or Delete event. In events obtained from a selected protocol, the entire sequence of consecutive events is defined with event names, definitions, concentrations and titration volumes. Name - Enter an event name of 1 to 10 characters. Short names (e.g. O instead of Open) are recommended. Comment - Further information can be entered into the text field. Select O2k-chamber A, B or both. The Event will be shown on plots for both or one selected chamber.

»Protocol events
Experimental codeAn experimental code can be entered in the Sample window, containing up to 10 digits.
Experimental log - DatLabExperimental log provides an automatically generated experimental protocol with detailed information about the O2k settings and calibrations, the Sample information and various Events. Time-dependent information can be viewed for a single chamber or both chambers. The filter can be selected for viewing minimum information, intermittent by default, or all information. The experimental log can be viewed and saved as a PDF file by clicking on [Preview].
Expert/inn/en-Workshop Medizintechnik Innsbruck AT
Export DL-Protocol User (*.DLPU)it is a function of DatLab (available from version 7.4 onwards) that enables the export of user specific protocols (DL-Protocol User) to the SUIT protocol folder from which they can be uploaded for subsequent measurements.
Extended abstractsIn the context of MiPevents, extended abstracts are accepted for preprint publication in MitoFit Preprints upon evaluation by the MitoFit Preprints Scientific Advisory Board. Publishing extended abstracts with MitoFit Preprints does not preclude later full journal publication, but will make your work fully citable, by assigning each manuscript a unique DOI number, and facilitate discovery and feedback.
FAT4BRAIN
FEBS 2019 Krakow PL
FEBS Workshop Ageing 2019 Innsbruck AT
FEMtech Internship for students
FI Helsinki Jacobs HT
FI Helsinki Mervaala E
FI Helsinki Pirinen E
FI Helsinki University of Helsinki
FI Helsinki Wartiovaara A
FI Helsinki Wikstroem M
FI Jyväskylä Kainulainen H
FI Oulu Kastaniotis A
FI Tampere Dufour E
FI Turku Nikinmaa M
FR Angers Andriantsitohaina R
FR Angers Gueguen N
FR Aubiere Sirvent P
FR Bordeau Di Rago JP
FR Bordeaux Devin A
FR Bordeaux Mourier A
FR Bordeaux Rossignol R
FR Dijon Leloup C
FR Evry Tardo-Dino PE
FR Fort de France Neviere R
FR Grenoble Saks VA
FR Grenoble Schlattner U
FR La Rochelle Rosenfeld E
FR Lille Boutry M
FR Lille Duez H
FR Lille Lancel Steve
FR Lille Vienne JC
FR Lyon Ovize M
FR Marseille Bioenergetics
FR Marseille Brasseur G
FR Marseille Denis M
FR Marseille Gregori G
FR Montpellier Chabi B
FR Montpellier Fauconnier J
FR Montpellier Prouteau-Angebault C
FR Montpellier Salmon JM
FR Paris Bouillaud F
FR Paris Wai T
FR Pessac Pasdois P
FR Plouzane Salin K
FR Saint Gilles Lefaucheur L
FR Strasbourg Blanc S
FR Strasbourg Zoll J
FR Suresnes Sabatini M
FR Toulouse Casteilla L
FR Toulouse Dray C
FR Toulouse Letellier T
FR Tours Dumas JF
FR Villeurbanne Romestaing C
Fatty acid oxidationFatty acid oxidation (β-oxidation) is a multi-step process by which fatty acids are broken down to generate acetyl-CoA, NADH and FADH2 for further energy transformation in the mitochondrial matrix. Whereas NADH is the substrate of CI, FADH2 is the substrate of Electron-transferring flavoprotein complex (CETF) which is localized on the matrix face of the mtIM, and supplies electrons from FADH2 to CoQ2. Before the mitochondrial transport for ß-oxidation, fatty acids (short-chain with 1-6, medium-chain with 7–12, long-chain with >12 carbon atoms) are activated by fatty acyl-CoA synthases (thiokinases) in the cytosol. For the mitochondrial transport of long-chain fatty acids the mtOM-enzyme carnitine palmitoyltransferase I (CPT-1; rate-limiting step in FAO) is required which generates an acyl-carnitine intermediate from acyl-CoA and carnitine. In the next step, an integral mtIM protein carnitine-acylcarnitine translocase (CACT) catalyzes the entrance of acyl-carnitines into the mitochondrial matrix in exchange for free carnitines. In the inner side of the mtIM, another enzyme carnitine palmitoyltransferase 2 (CPT-2) converts the acyl-carnitines to carnitine and acyl-CoAs, which undergo ß-oxidation in the mitochondrial matrix. Short- and medium-chain fatty acids do not require the carnitine shuttle for mitochondrial transport. Octanoate, but not palmitate, (eight- and 16-carbon saturated fatty acids) may pass the mt-membranes, but both are frequently supplied to mt-preparations in the activated form of octanoylcarnitine or palmitoylcarnitine.
File search - DatLabFile search yields a list of all files labelled by the experimental code in a selected directory . Click on the file to preview the experimental log. With File Search you can search in all folders and subfolders on your computer for DatLab files with a selected experimental code. The experimental code is entered in the DatLab file in the window "Experiment" ([F3]). When you click on a folder and press the button search, the DatLab file names will appear on the right window. Click on a DatLab file and further information (e.g. Sample information, Background information) will appear in the window below.
Filter Set AmR
Filter Set AmR.JPG
Filter Set AmR: Set of filters for the determination of H2O2 production with Amplex UltraRed. These filters should be used together with Fluorescence-Sensor Green. The filter set consists of 6 LED filters (round) and 6 photodiode filters (rectangular).
Filter Set MgG / CaG
Filter Set MgG CaG.JPG
Filter set MgG / CaG: Set of filters for the determination of concentraions of Mg2+ or Ca2+ with the fluorophores Magnesium green and Calcium green, respectively. These filters should be used together with Fluorescence-Sensor Blue or Smart Fluo-Sensor Blue. The filter set consists of 6 LED filters (round) and 6 photodiode filters (rectangular).
Filter Set Saf
Filter Set Saf.JPG
Filter set Saf: Set of filters for the (qualitative) determination of mitochondrial membrane potential with Safranin. These filters should be used together with Fluorescence-Sensor Blue or Smart Fluo-Sensor Blue. The filter set consists of 6 LED filters (round) and 6 photodiode filters (rectangular).
Filter-Cap
Filter-Cap.JPG
Filter-Cap: O2k-Fluo LED2-Module (O2k-Series D to G) sensors (Fluorescence-Sensor Green and Fluorescence-Sensor Blue) and O2k-FluoRespirometer (O2k-Series H to I) sensors (Smart Fluo-Sensor Green and Smart Fluo-Sensor Blue) are equipped with a removable Filter-Cap for exchange of optical filters for the optical pathways from the LED to the sample and from the sample to the photodiode.
Fischer 2021 MitoFit Fe liver
Fischer 2022 MitoFit Fe
Fluo calibration - DatLab
Fluorescence-Control Unit
Fluorescence-Control Unit lettered.jpg
Fluorescence-Control Unit with O2k-Front Fixation, Current-Control (O2k-Chamber A and B) for regulation of light intensity of the LED in the fluorescence sensors. This item is a standard component of the O2k-Fluorescence LED2-Module.
Fluorescence-Sensor Blue
Fluorescence-Sensor Green.JPG
Fluorescence-Sensor Blue: excitation LED 465 nm (dominant wavelength), photodiode, Filter-Cap equipped with Filter Set Saf for measurement of mitochondrial membrane potential with Safranin when delivered. The filter set Filter Set MgG / CaG for Magnesium green® / Calcium green® measurements is included.
Fluorescence-Sensor Green
Fluorescence-Sensor Green.JPG
Fluorescence-Sensor Green: excitation LED 525 nm (dominant wavelength), photodiode, Filter-Cap equipped with Filter Set AmR for Amplex UltraRed measurements when delivered.
Fluorescence-Service Box
Fluorescence-Service Box.JPG
Fluorescence-Service Box: for storage and shipment of theO2k-Fluo LED2-Module.
Flux / SlopeFlux / Slope is the time derivative of the signal. In DatLab, Flux / Slope is the name of the pull-down menu for (1) normalization of flux (chamber volume-specific flux, sample-specific flux or flow, or flux control ratios), (2) flux baseline correction, (3) Instrumental background oxygen flux, and (4) flux smoothing, selection of the scaling factor, and stoichiometric normalization using a stoichiometric coefficient.
Flux analysis - DatLabThe strategy of Flux analysis using DatLab depends on the research question and the corresponding settings applied in DatLab when recording the data with the O2k. Usng SUIT protocols, a sequence of respiratory steady-states is measured, marks are set, and numerical data are summarized in Mark statistics (F2). An AI approach is kept in mind when describing guidelines for evaluation of steady-states during data recording and analysis.
... further results