From Bioblast
Description
Octanoylcarnitine is a medium-chain fatty acid (octanoic acid: eight-carbon saturated fatty acid) covalently linked to carnitine, frequently applied as a substrate for fatty acid oxidation (FAO) in mitochondrial preparations.
Abbreviation: Oct
Reference: pdf
Application in HRR
- Oct: Octanoylcarnitine ((Β±)-Octanoylcarnitine chloride; C15H30NO4Cl), BioTrend (brand: APExBIO Technology): B6371-50 mg, store at -20 Β°C, CAS: 14919-35-8, M = 323.86 gΒ·mol-1
- Alternative: TOCRIS Bioscience, Cat. No. 0605, 50 mg, store at RT, desiccate); M = 323.85 gΒ·mol-1.
- General tips: For obtaining a higher solubility, warm the tube at 37 β and shake it in the ultrasonic bath for a while.
- Preparation of 100 mM stock solution (dissolved in H2O or DMSO, can differ from batch to batch - check Certificate of Analysis):
- Weigh 50 mg of octanoylcarnitine and dissolve in 1543 Β΅L H2O or DMSO.
- Divide into 0.2 mL portions.
- Store at -20 Β°C.
- Note 2024-03-26: According to the information provided by the supplier (BioTrend) is the solubility of Octanoylcarnitine in DMSO and H2O comparable ( β₯36.9 mg/mL in DMSO; β₯18.6 mg/mL in EtOH; β₯37.4 mg/mL in H2O), therefore we consider more rational approach to use H2O as a solvent in OROBOROS Lab because of better handling at room temperature. Disadvantage of DMSO: freezing point of +18.55 Β°C (65.4 Β°F). When using DMSO as solvent, make sure to bring the stock solution to room temperature before filling the syringe, which is important to avoid blocking the needle.
- communicated by Sobotka Ondrej (talk) 17:46, 26 March 2024 (CET)
- Β» Oroboros manual titrations MiPNet09.12 Manual titrations of SUIT chemicals
- Titration volume (2-mL O2k-chamber): 10 Β΅L using a 25 Β΅L Hamilton syringe.
- Titration volume (0.5-mL O2k-chamber): 2.5 Β΅L using a 10 Β΅L Hamilton syringe
- Experimental concentration: 0.5 mM in MiR05 or MiR06. Optimum Oct concentrations have to be evaluated, particularly in media with BSA concentrations different from MiR05.
MitoPedia topics:
Substrate and metabolite
Labels:
MitoPedia:FAT4BRAIN