Cover-Slip\black

                  

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Cover-Slip\black

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MitoPedia O2k and high-resolution respirometry: Oroboros Open Support 

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Description

A Cover-Slip should be placed on top of the Oroboros Stopper to minimize contamination and evaporation of liquid extruding from the capillary of the stopper. The Cover-Slips do not exert any direct effect on oxygen backdiffusion into the Oroboros chamber. Use the the Cover-Slip\black to avoid light penetration and disturbance of fluorescence signals and generally for optical measurements in the Oroboros.

Reference: Product details and purchase information

Technical support

• Cover-Slips cannot be placed on top of the stoppers in Oroboros MultiSensor applications with additional sensors inserted through the stopper, or while TIP2k needles are inserted into the Oroboros chamber.
• The black cover-slips replaced the previous perspex Cover-Slips (Product ID 24410-01). They are functionally identical, but the black cover slips take into account the optical properties of the Oroboros FluoRespirometer and prevent light from penetrating through the capillaries of the stoppers when using optical sensors.

Additional resources

» MiPNet22.11 O2k-FluoRespirometer manual

Keywords

»Bioblast links: Chamber volume - >>>>>>> - Click on [Expand] or [Collapse] - >>>>>>>

» Volume

• Units: Liter [L]; 1 L = 1 dm³ = 10^-3 m³; 1 mL = 1 cm³; 1 µL = 1 mm³

» Oroboros chamber

» The experimental chamber in the instrumental Oroboros Chamber

» Chamber sV

» Calibration of Oroboros chamber volume

• On chamber volume

» Oroboros chamber

» Chamber volume

» Oroboros paradigm shift

» Cell count and normalization in HRR

» Open chamber

» Closed chamber

• Components of the experimental Oroboros chambers

» Stirrer-Bar\white PVDF\15x6 mm

» Stirrer-Bar sV\white PVDF\11.5x6.2 mm

» Stopper\black PEEK\conical Shaft\central Port

» Stopper sV\black PEEK\conical Shaft\central Port

» O-ring\Viton\12.5x1 mm

» O-ring sV\Viton\9.5x1 mm

» Volume-Calibration Ring

» Volume-Calibration Ring sV

» Stopper-Spacer

» Cover-Slip\black

» O2k-Chamber Holder

» O2k-Chamber Holder sV

» OroboPOS-Holder

» OroboPOS-Holder sV

• Maintencance

» Oroboros chamber cleaning and ISS

» Quality control: Oroboros chamber test

MitoPedia O2k and high-resolution respirometry: Oroboros hardware, Oroboros Open Support, O2k-Respirometry, O2k-FluoRespirometry