Open chamber

                  

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Open chamber

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MitoPedia O2k and high-resolution respirometry: O2k-Open Support 

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Description

The term "open Oroboros chamber" refers to a situation in which the liquid phase is allowed to equilibrate with a gas phase, but the stopper is partially inserted using the Stopper-Spacer.

Abbreviation: O

Reference: System, Gnaiger 2001 Respir Physiol

Application in HRR

The liquid phase is defined as the experimental system, which is then an open system with exchange of oxygen with the gas phase, finally resulting in a stable oxygen signal with a slope over time of zero. Insert the stopper fully, siphon off any liquid from the receptacle of the stopper, and remove the stopper to a position defined by the Stopper-Spacer to include an optimum volume of the gas phase above the stirred liquid phase.

Reconsiderations

The experimental volume of the chamber is the homogenously stirred liquid phase. When the stopper is inserted into the volume-calibrated position and the medium fills the chamber including the stopper capillary, then the experimental volume V does not include the dead volume V_stc of the stopper capillary, including a small meniscus on top of the capillary required to visually observe complete filling of the capillary. When moving the stopper off the volume-calibrated position, a stirred volume V´ = V+V_stc is obtained, since the medium V_stc is drained into the chamber when moving the stopper upwards.

Open O: If we adhere to textbooks, then any titration into the Oroboros chamber is 'opening' the system for mass transfer. This type of 'opening' disturbs the interpreation of the time-derivative of oxygen concentration as oxygen flux (or H₂O₂ flux), visible as apparent titration spikes in the time derivative. Mathematical corrections for this type of opening the chamber is possible when interpreting the oxygen concentration in the system in terms of internal and external causes of concentration change, d_ic_O2 and d_ec_O2.

To avoid ambiguities, O should stand for "stopper is taken Off the volume-calibrated position.

» Compare: Closed chamber

Keywords

»Bioblast links: Chamber volume - >>>>>>> - Click on [Expand] or [Collapse] - >>>>>>>

» Volume

• Units: Liter [L]; 1 L = 1 dm³ = 10^-3 m³; 1 mL = 1 cm³; 1 µL = 1 mm³

» Oroboros chamber

» The experimental chamber in the instrumental Oroboros Chamber

» O2k-Chamber sV

» Calibration of Oroboros chamber volume

• On chamber volume

» Oroboros chamber

» Chamber volume

» Oroboros paradigm shift

» Cell count and normalization in HRR

» Open chamber

» Closed chamber

• Components of the experimental Oroboros chambers

» Stirrer-Bar\white PVDF\15x6 mm

» Stirrer-Bar sV\white PVDF\11.5x6.2 mm

» Stopper\black PEEK\conical Shaft\central Port

» Stopper sV\black PEEK\conical Shaft\central Port

» O-ring\Viton\12.5x1 mm

» O-ring sV\Viton\9.5x1 mm

» Volume-Calibration Ring

» Volume-Calibration Ring sV

» Stopper-Spacer

» Cover-Slip\black

» O2k-Chamber Holder

» O2k-Chamber Holder sV

» OroboPOS-Holder

» OroboPOS-Holder sV

• Maintencance

» Oroboros chamber cleaning and ISS

» Quality control: Oroboros chamber test

Bioblast links: Oxygen signal - >>>>>>> - Click on [Expand] or [Collapse] - >>>>>>>

Specific

» Oxygen signal

» O2k signal line

O2k-Procedures

» Air calibration

» Zero calibration

» High signal at zero oxygen

» POS calibration - static

» POS calibration - dynamic

» DL-Protocols

» Oxygen sensor test

» Stirrer test

» Open chamber

» Closed chamber

MiPNet O2k-Procedures

» POS-calibration

» O2k-Manual: POS-service

» O2k-cleaning

» O2k-Series G and DatLab 6: Calibration

General

» Oxygen, dioxygen, O₂

» Oxygen calibration - DatLab

» Oxygen solubility

» Oxygen solubility factor

» Oxygen pressure

» Concentration

» Activity

» Pressure - Pascal

» Barometric pressure

» High-resolution respirometry

» OroboPOS

» Polarographic oxygen sensor

» MitoFit Quality Control System

» Sensitivity

Other keyword lists

» Keywords: Concentration and pressure

Oroboros quality management from the O2k to NextGen-O2k
Gnaiger Erich 2020-09-07