Search results

...'' = ''E''). The biochemical coupling efficiency is independent of kinetic control by the phosphorylation system. == Keywords ==

...ng-control ratio combines the effects of coupling (''L/E''), physiological control of energy demand, and limitation by the OXPHOS capacity. == Keywords ==

...rol ratio''' or LEAK/OXPHOS coupling-control ratio combines the effects of coupling (''L/E'') and limitation by the phosphorylation system (''P/E''); ''L/P'' = == Keywords ==

...6 Am J Physiol Cell Physiol|Steinlechner et al 1996]]). Compare [[ROUTINE-control ratio]] (''R/E'') [[Gnaiger 2008 POS|(Gnaiger 2008)]]. == Biochemical coupling efficiency: from 0 to <1 ==

...or [[LEAK respiration]]) as a fraction of [[ET capacity]]. The net ''P/E'' control ratio remains constant, if [[dyscoupling]] is fully compensated by an incre == Keywords ==

...or [[LEAK respiration]]) as a fraction of [[ET capacity]]. The net ''R/E'' control ratio remains constant, if [[dyscoupling]] is fully compensated by an incre == Keywords ==

|description=[[File:J(R-L).jpg|50 px|R-L ROUTINE-coupling efficiency]] ...The background state is the [[LEAK respiration|LEAK]] state, and the flux control variable is stimulation to ROUTINE respiration by physiologically controlle

...'P/E'']], and two ''CCR'' for living cells, [[L/E |''L/E'']] and [[ROUTINE-control ratio |''R/E'']]. |info=[[Flux control ratio]], [[Gnaiger 2020 BEC MitoPathways]]

...r]]s (e.g. [[CCCP]]). [[Coupling-control protocol]]s induce these coupling-control states sequentially at a constant [[electron-transfer-pathway state]]. '''Coupling-control states:''' [[Image:P.jpg|link=OXPHOS capacity|OXPHOS capacity]] [[Image:R.j

...E respiration]] ''R'' and (noncoupled) [[ET capacity]] ''E''. The ''R/E'' control ratio is an expression of how close ROUTINE respiration operates to ET capa == Boundaries of the ''R/E'' control ratio ==

...f oxygen consumption in ''L'' and ''E'' sequentially. The ''L/E'' coupling-control ratio is an index of [[uncoupling]] or [[dyscoupling]] at constant ET-capac == Keywords ==

|description=[[Image:j(E-R).jpg|50 px|E-R control efficiency]] The '''''E-R'' control efficiency''', ''j_E-R'' = (''E-R'')/''E'' = 1-''R/E'', is an exp |info=[[Flux control efficiency]]

...ontrol over OXPHOS capacity. ''j_E-P'' increases with increasing control of the phosphorylation system over OXPHOS capacity. ''j_E-P'' = 1 |info=[[Flux control efficiency]], [[Gnaiger 2020 BEC MitoPathways]]

...erts a limiting effect on OXPHOS capacity. In addition, ''E-P'' depends on coupling efficiency, since ''P'' aproaches ''E'' at increasing uncoupling. == Keywords ==

|description=A '''metabolic control variable''' ''X'' causes the transition between a [[background state]] Y (b |info=[[Flux control efficiency]]

== Keywords == {{Template:Keywords: Coupling control}}

...ency |''P-L'' control efficiency]] if ''P=E'' as evaluated in a [[coupling-control protocol]]. |info=[[Flux control efficiency]]

...e consisting of three coupling control states. The term '''phosphorylation control protocol''', PCP, has been introduced synonymous for CCP. ...sted to replace the term 'phosphorylation control protocol' by '''coupling-control protocol, CCP'''.

== Keywords == {{Template:Keywords: Coupling control}}

...ontrol efficiencies''' express the control of respiration by a [[metabolic control variable]], ''X'', as a fractional change of flux from ''Y_X'' to ...to [[elasticity|elasticities]] of [[metabolic control analysis]], the flux control efficiency of ''X'' upon background ''Y_X'' is expressed as the c

...''E-P'' control efficiency]], ''j_E-P'' = 1-''P/E''. The ''P/E'' control ratio increases with increasing capacity of the phosphorylation system up t ...ng]] of [[OXPHOS]], but mainly results from a diversity in the [[ET-excess control efficiency E-P]] in different species, tissues and cell lines.

| Coupling control protocol (ceCCP) in intact cells. | Coupling control protocol (ceCCP) in intact cells using TIP2k for automatic uncoupler titrat

...ol efficiency]] ''j_P-L'' and [[E-L coupling efficiency |''E-L'' coupling efficiency]] ''j_E-L''. These normalized flux ratios are the basi == Keywords ==

...e=MitoEAGLE preprint 2018-02-18(21) The protonmotive force and respiratory control. |keywords=Mitochondrial respiratory control, coupling control, mitochondrial preparations, protonmotive force, chemiosmotic theory, oxida

...trol ratios''' ''FCR''s are ratios of oxygen flux in different respiratory control states, normalized for maximum flux in a common reference state, to obtain ...SUIT protocol]]) provide an internal normalization, expressing respiratory control independent of mitochondrial content and thus independent of a marker for m

...sons, ''RCR'' should be replaced by the [[E-L coupling efficiency |''E-L'' coupling efficiency]], 1-''L/E'' [4]. :::# Gnaiger E (2020) Mitochondrial pathways and respiratory control. An introduction to OXPHOS analysis. 5th ed. BEC 2020.2. - [[Gnaiger 2020 B

...Boushel RC (2011) Conserved mitochondrial coupling and adaptive substrate control in Inuit and Caucasians: Acclimatization to active life styles in the Arcti |keywords=Haplotypes

...on was not inhibited in any of the coupling control states, hence coupling control efficiencies were not affected by MgG. |keywords=ATP, ATP production, high-resolution respirometry, Magnesium Green, mitocho

== Keywords == {{Template:Keywords: Coupling control}}

...T, Gnaiger E (2022) Bioenergetic fingerprinting with coupling and pathway control of coenzyme Q redox state and respiration in permeabilized HEK 293T cells. ...ing is challenged with targeting specific segments of pathway and coupling control of oxidative phosphorylation (OXPHOS) [1].

...Leung KH, Hinkle PC (1975) Reconstitution of ion transport and respiratory control in vesicles formed from reduced coenzyme Q-cytochrome c reductase and phosp ...ansient stimulation of electron transfer. The results indicate that energy coupling can be observed with isolated reduced coenzyme Q-cytochrome c reductase if

== Keywords == {{Template:Keywords: Coupling control}}

...on was not inhibited in any of the coupling control states, hence coupling control efficiencies were not affected by MgG. |keywords=ATP, ATP production, high-resolution respirometry, Magnesium Green, mitocho

...ancock CR (2017) Effects of curcumin and ursolic acid on the mitochondrial coupling efficiency and hydrogen peroxide emission of intact skeletal myoblasts. Bio ...lood glucose control. Our findings also show that changes to mitochondrial coupling efficiency after curcumin pretreatment may go undetected unless cells are a

|title=Sumbalova Z, Fasching M, Gnaiger E (2011) Substrate control in mitochondrial respiration and regulation of mitochondrial membrane poten ...Media:MiPNet14.05.jpg|Sumbalova Z, Fasching M, Gnaiger E (2011) Substrate control in mitochondrial respiration and regulation of mitochondrial membrane pote

...LHD, Doerrier C, Moore AL, Rich PR, Gnaiger E (2021) Coupling and pathway control of coenzyme Q redox state and respiration in isolated mitochondria. https:/ ...ww.mitofit.org/images/9/91/Komlodi_2021_MitoFit_Q.pdf Coupling and pathway control of coenzyme Q redox state and respiration in isolated mitochondria]

...-specific dysregulation of mitochondrial respiratory capacity and coupling control in colon-26 tumor-induced cachexia. Am J Physiol Regul Integr Comp Physiol ...findings suggest that mitochondrial function is subject to tissue-specific control during cancer cachexia, whereby remodeling in WAT and liver arise early and

...''P/L''. Under these conditions, the respiratory control ratio and ''P-L'' control efficiency are related by a hyperbolic function, ''j_P-L'' = 1-RC » [[#Cell ergometry: OXPHOS-control and ET-coupling efficiency |'''MiPNet article''']]

...ased (''P'' = 0.031), leading to a significant increase in the respiratory control ratio (''P'' = 0.016). |keywords=Exercise training, Aerobic metabolism, Chronic hypoxia, Women, VO2max, Ener

...production. Despite substantial differences in the [[respiratory acceptor control ratio]], ranging from 4 to 11 in the different experimental groups, a fixed |keywords=Aging, Coupling state, Mitochondria, Oxidative stress, Primary human fibroblast, Respiratio

...PHOS capacity is expressed as the [[OXPHOS-control ratio]] (''P/E'' [[flux control ratio]]). (''2'') OXPHOS capacity is expressed per tissue or cell mass, int == Keywords ==

=== Coupling-control protocol === ...ler titrations after inhibition of respiration by oligomycin in [[coupling-control protocol]]s with living cells yield the sequence of [[ROUTINE respiration]]

...m alterations in mitochondrial content. LEAK control coupling, respiratory control ratio, and oligomycin-induced LEAK respiration, all measures of mitochondri |keywords=High altitude (HA), Exercise capacity

...ail to evaluate even minor alterations in respiratory coupling and pathway control patterns. ...⁺⁾. The [[PB-Module]] extends HRR to PhotoBiology with accurate control of the light intensity and measurement of photosynthesis. The O2k and the N

...) High levels of whole-body energy expenditure are associated with a lower coupling of skeletal muscle mitochondria in C57Bl/6 mice. Metabolism 59:1612-8. ...coenzyme A as substrate. These results indicate that reduced mitochondrial coupling is associated with positive residual energy expenditure and high rates of t

...iethuechter A, Fasching M, Gnaiger E (2011) Coupling control and substrate control of mitochondrial membrane potential and respiration in the mouse brain, and |keywords=High-resolution respirometry, OXPHOS, mitochondrial membrane potential, ROS

...is controlled by cellular energy demand, energy turnover and the degree of coupling to phosphorylation (intrinsic [[uncoupling]] and pathological [[dyscoupling ::::* DL-Protocol for coupling control with living cells and cell viability module (ce-pce): [[SUIT-003 O2 ce-pce

|title=Ragan CI, Hinkle PC (1975) Ion transport and respiratory control in vesicles formed from reduced nicotinamide adenine dinucleotide coenzyme ...y valinomycin plus nigericin and K+ ions. The results indicate that energy coupling can be observed with isolated NADH-coenzyme Q reductase if the enzyme compl

...in complex II-linked respiration. Coupling control ratios showed increased coupling efficiency for both complex I- and II-linked substrates in hypoxia-exposed ...oxygenation period increases substrate-stimulated respiratory capacity and coupling efficiency. Such effects are accompanied by up-regulation of mitochondrial

...loading is related to decreased mitochondrial ROS production and increased coupling efficiency in myocardium of human failing hearts, suggesting a novel pathom |keywords=Heart failure, Heart transplantation, High-resolution respirometry, Left ve

...s reductions in: maximal mitochondrial respiration capacity, mitochondrial coupling and mitochondrial content. |keywords=Mitochondrial content, Mitochondrial coupling, Mitochondrial homeostasis, Mitochondrial respiration, Muscle wasting, Oxid

...LHD, Doerrier C, Moore AL, Rich PR, Gnaiger E (2021) Coupling and pathway control of coenzyme Q redox state and respiration in isolated mitochondria. Bioener ...s determined by cyclic voltammetry. The voltammogram also provides quality control of the Q-sensor and reveals chemical interferences.

...uence of titrations, e.g. NS(GM), NS(PM), FNSGp(PGM). The coupling/patwhay control diagram in the Figure shows a protocol of SUIT category FNS(PM). :* '''Coupling-control protocols''' (CCP) are a special case of SUIT protocols primarily applied t

...cantly lower in burn victims for 1-year postinjury. Mitochondrial coupling control is diminished for up to 2 years postinjury in burn victims, resulting in gr |keywords=skeletal muscle, ucnoupling, burns, trauma

...ower leak respiration and leak-related coupling control ratio than healthy control subjects. There was no significant change in measures of PBMC respiration f |keywords=Mitochondria, Respiratory, Type 2 diabetes, Dysfunction, Peripheral blood m

...l 10d post burn, where both respiratory capacity (''P''<0.05) and coupling control (''P''<0.05) were significantly lower than sham. These data highlight a dif |keywords=Apoptosis, Burn, Hypermetabolism, Mitochondria, Skeletal muscle

...ulations indicated an enzyme defect in β-oxidation, and that mitochondrial coupling efficiency of electron transfer during β-oxidation particularly in fat oxidation (coupling efficiency and relative flux) were found.

...P, Fasching M, Gnaiger E (2011) Mitochondrial membrane potential, coupling control, H₂O₂ production, and the upper limit of mitochondria

...one C, Kante F, Folkow LP, Hazlerigg DG, West AC, Wood SH (2024) Circadian coupling of mitochondria in a deep-diving mammal. J Exp Biol 227:jeb24699. https://d ...sts and found a circadian variation in mitochondrial activity, with higher coupling efficiency of complex I coinciding with the trough of arntl expression. The

...d (direct) channeling of that fraction of ADP to mitochondria. Compared to control, the direct ADP transfer was increased in MDX ventricles. In conclusion, ou |keywords=Mitochondria, Adenosine 5′-triphosphatase, Muscle, Respiration, Regulatio

...delivery suite, with enhanced coupling, as indicated by higher respiratory control ratios, than in fresh placental samples transported to the laboratory on ic |keywords=Placenta

|title=Krumschnabel G, Schwarzbaum PJ, Wieser W (1994) Coupling of energy supply and energy demand in isolated goldfish hepatocytes. Physio ...the concentration of ATP in the hepatocytes dropped within 30 min from the control steady state to a new, lower, steady state that was maintained for at least

...P) in intact cells to evaluate effects on ROUTINE respiration, biochemical coupling efficiency and extracellular ROS production under physiological conditions; == Keywords ==

...] Electron transfer from beta-oxidation and TCA cycle and impact of OXPHOS coupling on NADH and coenzyme Q redox states. ...sh from different fuel substrates of the ETS or electron pull modulated by coupling and corresponding energy demand. Special emphasis on the interaction betwee

...substrate oxidation rates, leak respiration rates or coupling (respiratory control ratios). Increased rates of ROS production did not lead to damage of protei ...lase, Mitochondria, Oxidative damage, Reactive oxygen species, Respiratory control ratio, Temperature

...gnificantly higher OXPHOS Control Efficiency, OXPHOS Capacity and Coupling Control Ratios (i.e., LEAK/OXPHOS). There was no significant change to citrate synt |keywords=Fish, Heart, Low oxygen, Oxidative phosphorylation, Red muscle

...M, Tiret L, <u>Pilot-Storck Fanny</u> (2022) ''Hacd1'' and ''Hacd2'' genes control mitochondrial energetic efficiency through the modulation of mitochondrial |abstract=Mitochondrial energy efficiency involves optimal coupling between oxidative processes and ADP phosphorylation, which implies an advan

...art failure, RCR, Respiratory control ratio, SC CCF, Supercomplex coupling control factor, Heart failure, High-resolution respirometry, Mitochondrial-targeted

...ratory chain function remained unaltered, as both coupling and respiratory control did not change in response to hypoxic exposure. High altitude acclimatizati |keywords=High altitude acclimatization

...rumschnabel G, Biasi C, Schwarzbaum PJ, Wieser W (1996) Membrane-metabolic coupling and ion homeostasis in anoxia-tolerant and anoxia-intolerant hepatocytes. A ...ecies did chemical anoxia induce a change in pump density. Other potential control mechanisms are briefly discussed.

...drial outer membrane (mtOM) for ADP, is crucial for the efficiency of this coupling. To validate if tubulin participates in this regulation, reconstitution exp |keywords=Bioenergetics, Muscle cells, Energy metabolism, Tubulin, Dissertations

...LHD, Doerrier C, Moore AL, Rich PR, Gnaiger E (2021) Coupling and pathway control of coenzyme Q redox state and respiration in isolated mitochondria. Bioener ...LHD, Doerrier C, Moore AL, Rich PR, Gnaiger E (2021) Coupling and pathway control of coenzyme Q redox state and respiration in isolated mitochondria. Bioener

...ity from mouse to man, and evaluation of altered mitochondrial respiratory control patterns in health and disease'' ([[Gnaiger 2009 Int J Biochem Cell Biol|Gn ...the backbone of the MiPMap project. The MiPMap labels can be considered as keywords which can be attached to various sources of information (publications). Wit

|abstract=Coupling of [[OXPHOS]] represents a complex concept. '''Uncoupler''' titrations prov ...link«]]</ref>,<ref>Gnaiger E (2020) Mitochondrial pathways and respiratory control. An introduction to OXPHOS analysis. 5th ed. Bioenerg Commun 2020.2. https:

...iu L, Fu T, Kong Y, Zhou Q, Vega RB, Zhu MS, Kelly DP, Gao X, Gan Z (2016) Coupling of mitochondrial function and skeletal muscle fiber type by a miR-499/Fnip1 ...contractile proteins of oxidative-type fibers. However, the mechanism for coupling of mitochondrial function to the muscle contractile machinery during fiber

...ansfer to phosphorylation of ADP. To secure full accessibility of any flux control variables (substrates, ADP, etc.) to the mt organelle, mitochondria are eit ...ibility of complete mt-yield and preservation of mitochondrial respiratory control compared to permeabilized fibers.

...livo Eleonora]]Baglivo E, Cardoso LHD, Cecatto C, Gnaiger E (2022) Quality control of mitochondrial respiration medium in high-resolution respirometry with li ...ochondrial respiratory research. A frequently neglected problem is quality control of chemicals and incubation media. Mitochondrial respiration medium MiR05 i

...tial]] is almost fully collapsed and provides a reference state for [[flux control ratio]]s. == Consequences for evaluation of coupling ==

...supported by PMS and the respiratory control ratio (index of mitochondrial coupling) for both PMS and PCM were lower following the clamp (P<0.05). IRS1 mRNA de ...induced H₂O₂ production and a concurrent decrease in coupling of mitochondrial respiration with ATP production in a cohort of healthy pre

...on in OXPHOS efficiency. However, with anoxic transition, STZ-diabetic and control heart tissues showed similar ATP hydrolysis capacities through reversal of |keywords=Diabetic cardiomypathy, Anoxia, Efficiency, Mitochondria, Oxidative phospho

...tioning mitochondria-bound hexokinase as a hub for intracellular signaling control. This article is protected by copyright. All rights reserved. |keywords=Mitochondria, Brain, Calcium, Development, Hexokinase, Redox balance

...maintained in all treatments, as deduced from high respiratory uncoupling control ratios. Bcl-2 over-expression did not prevent apoptosis after gemcitabine-t |keywords=Apoptosis, Dexamethasone, Gemcitabine, Mitochondrial respiratory control, Cell cycle

...embrane H+ conductance and disturbs the protein-linked functions in energy coupling. This is most probably essential for the short-term toxicity of phytanic ac |keywords=ADP/ATP carrier, Branched-chain fatty acid, Mitochondria, Neurodegeneration

...''_O2max and an ~50 % increase (''P'' < 0.05) of the respiratory coupling ratio (RCR) combined with lower levels of UCP3 and PGC-1αmRNA levels (''P' ..., High-fat diet, Nutrient restriction, Maternal diet, Respiratory coupling control ratio

...Calbet J, Christiansen M, Helge JW, Saltin B, Boushel R (2013) Biochemical coupling efficiency in permeabilized fibers from arm and leg muscle in Inuit versus ...e in fatty acid oxidation approaching the level of the Inuit, yet coupling control of oxidative phosphorylation was conserved. Our findings reveal that couple

...acity and OXPHOS coupling efficiency, and induced changes in the substrate control ratio. Altogether, our results suggested that ''E. uniflora'' impairs the m |keywords=Mitochondrial dysfunction, Oxidative phosphorylation, Essential oil, Bioene

...n. In addition, we found that the acceptor control ratio, representing the coupling between oxidation and phosphorylation, was significantly upregulated by CL. |keywords=Biological therapies, Mitochondria, Oxidative phosphorylation, Vascular bio

...e (SDH) or Complex II. SDH is located at the inner mitochondrial membrane, coupling the oxidation of succinate to fumarate in the tricarboxylic acid cycle (TCA ...e; noncancerous), LNCaP (prostate; cancer), and HEK293T (embryonic kidney; control).

...evere burn trauma on liver mitochondrial respiratory capacity and coupling control as well as the signaling events underlying these alterations. ...he respiratory control ratio for ADP (p = 0.04) and the mitochondrial flux control ratio (p = 0.03) were elevated in the liver of burned animals. Complex

..., Pollheimer J, Moser PL, Zeimet AG, Offner FA, Gnaiger E (2006) Preserved coupling of oxidative phosphorylation but decreased mitochondrial respiratory capaci ...a significant decline of respiratory capacity (''p'' < 0.05). Respiratory control ratios (i.e., uncoupled respiration at optimum carbonyl cyanide p-trifluoro

...ransfer chain, ETC) is a misnomer. Understanding mitochondrial respiratory control has suffered greatly from this inappropriate terminology, although textbook ...her flavoproteins, providing multiple entries into the Q-junction with two coupling sites downstream (Gnaiger 2014).

== Keywords == {{Template:Keywords: Q}}

...NA. Improvement of the OXPHOS efficiency was stronger in APP cells than in control cells. In APP cells, the GBE-induced amelioration of oxygen consumption mos |keywords=Ginkgo biloba extract, Alzheimer's disease,

...SH-SY5Y cells, with basic information of respiration in different coupling control states including ROUTINE, LEAK, and maximal electron transport (ET) capacit |keywords=Bioenergetics, Intact cells, Mitochondria, Respiration, SH-SY5Y

...rel JC, Hacini R, Chavanon O, Imbeaud S, Saks V, Pison C (2005) Functional coupling of adenine nucleotide translocase and mitochondrial creatine kinase is enha ...itochondrial respiration by creatine linked to a more efficient functional coupling adenine nucleotide translocase-mitochondrial creatine kinase, resulting in

...msroth S, Antretter H, Höfer D, Gnaiger E (2011) Mitochondrial respiratory control and early defects of oxidative phosphorylation in the failing human heart. ...identical in the four cardiac chambers of chronic heart failure patients. Coupling was preserved in heart disease and chronic heart failure, in contrast to th

...s predicts that increased levels of inorganic phosphate in TAC compared to control animals kinetically impair the myosin ATPase crossbridge cycle in decompens |keywords=Heart failure, Cardiac energetics, Mechano-energtic coupling, Trans aortic constriction, Ejection fraction, Computational modeling, Card

...inclusions shown by electron microscopy, indicate that Mib-CK function and coupling to oxidative phosphorylation (OXPHOS), is impaired in these abnormal mitoch |keywords=Guanidino propionic acid; Creatine; Mitochondrial inclusion body; Creatine

...onsumption of permeabilized acinar cells. These results suggest a stronger coupling between respiration and oxidative phosphorylation in sucrose-based solution |keywords=Pancreatic acinar cells; Mitochondria; Oxygen consumption; Respiratory stat

...everal skeletal muscle adaptations, highlighting the role of mitochondrial coupling as a critical factor for maximal exercise capacities. These results emphasi |keywords=2,4-dinitrophenol, Mitochondrial uncoupling, Exhaustive exercise Maximal ru

...potential. With these two properties of mitochondria we can determine the coupling state of respiration and the impact of the mitochondrial substrate on the m ...estigated Complex I and II and a combination of I+II in different coupling control levels.

..., maximal mitochondrial respiratory capacity and oxidative phosphorylation coupling efficiency decreased during acclimation to higher temperatures, suggesting ...=G × E effects, Oroboros Oxygraph 2k, Climate variability hypothesis, Flux control factor, Mitochondrial respiration, Thermal tolerance

...both normoxic and anoxic trout cells, whereas in goldfish hepatocytes the coupling of K+ fluxes was not affected by the rise of Ca2+i |keywords=goldfish, rainbow trout, lactate, cyclosporine A, fructose, metabolic depre

...bo-treated SuHx rats (0.12 ± 0.01 vs. 0.15 ± 0.01 U citrate synthase/mg in control, P < 0.05), and estrogen attenuated the decrease. Mitochondrial quality and |keywords=Cardiopulmonary hemodynamics, Mitochondria, Sex differences

...a kinetic model of homogenous Cyt ''c'' depletion. Comparison to Cyt ''c'' control of respiration in CIR-injured myocardial fibers indicated heterogeneity of |keywords=Heart preservation, Complex I injury, Permeabilized myocardial fibers

...was removed from the diet, the running distance and speed decreased to the control level, despite the improved endurance achieved during nitrate supplementati |keywords=Endurance, Excitation-contraction coupling, Exercise, Oxidative capacity

...(OXPHOS) due to diminished control over it by ADP and impaired functional coupling of adenylate kinase to OXPHOS. In parallel, reduced tissue content of cytoc |keywords=Mitochondria; Overtraining; Oxidative phosphorylation; Rat Myocardium

...nd OXPHOS state and ''P-L'' control efficiency (1-''L''/''P'') compared to control cells, as characterized previously in detail [1]. ...that mitochondria in our model had decreased respiration rate and impaired coupling efficiency, it is important to additionally clarify the relationship betwee

...s, media formulation, living cells, high-resolution respirometry, coupling control, ROUTINE respiration, LEAK respiration, electron transfer capacity, residua |topics=Coupling efficiency;uncoupling

...ared different approaches to assess mitochondrial density and suggest flux control ratios as a valid method to normalize respiration to mitochondrial density. ...significant differences of CI- and CII-linked OXPHOS capacity and coupling control between motor cortex, striatum, hippocampus and pons of naïve mice. CI-lin

...were determined. Mitochondrial respiratory function (assessed by acceptor control ratio (ACR)) was impaired in BMI(L) (2.2+/-0.6) compared with both BMI(N) ( |keywords=COPD

...ectrochemical proton gradient is increased to a maximum, exerting feedback control by depressing oxygen flux to a level determined mainly by the proton leak a ...logical confusion persisting in the scientific literature, the respiratory coupling states of LEAK respiration, [[OXPHOS capacity]] and [[ET capacity]] are dis

...sed at 24 hpf. 70 zebrafish embryos were randomly divided in three groups; Control Group (C); MPTP Group (MPTP), treated with MPTP (600 μM), and aMT Group (a ...f alphasynuclein and iNOS increased in 72 hpf embryos treated with MPTP vs control; this rise was abrogated with the administration of aMT. A significant incr

...d a significant difference in the LEAK respiration state (P<0.05) and LEAK control ratio (P<0.01) between young and old LCR rats (L: 8.83 ± 1.8 vs. 9.67 ± 1 ....67), and CII (r = 0.44; P<0.01 for all), and negatively with the coupling control ratios L/E (r = -0.54) and P/E (r = -0.52; <0.01 for

...mains incomplete without a complementary thermodynamic analysis of kinetic control in energetically coupled pathways. |keywords=Twin-Flow

...xidative phosphorylation or electron transport, LEAK respiration, coupling control, or phosphorylation efficiency. Hypoxia acclimation also had no effect on m |keywords=Energy metabolism, Free radicals, Oxidative stress, Teleost, Ampliflu Red

...pling efficiency were not affected by SuHx or genotype. A higher substrate control ratio for succinate was observed in SuHx fibers and attenuated in ST2<sup>- |keywords=Diaphragm, Mitochondria, Pulmonary hypertension, Respirometry, SU5416

...dox states of Q_ra are sensitive to pathway control and coupling control of mitochondrial electron transfer and [[OXPHOS]]. (''2b'') The Q<sub>ia</s == Keywords ==

...TP/O ratios. Intactness of the outer mitochondrial membrane and functional coupling between mitochondrial creatine kinase and oxidative phosphorylation were ve |keywords=Freeze–thawing, Heart and skeletal muscles, Human biopsies, Mitochondrial

...ned complementary to mitochondrial [[coupling-control state]]s. [[Coupling-control state]]s require [[Electron-transfer-pathway state|ET-pathway competent sta ...are defined in [[mitochondrial preparations]] complementary to [[coupling-control state]]s in mitochondrial physiology.

...h LEAK respiration rates within liver and muscle mitochondria, and a lower coupling of muscle mitochondria. These observations, combined with the inability of ...words=Ecophysiology, Food intake, Global warming, Proton leak, Respiratory control ratio, Respiration rate

...for ET_H and ST_H; ''N''=7 and 7), and higher coupling control of oxidative phosphorylation. The enhanced lipid OXPHOS capacity was mainly ...ng, Human skeletal muscle, Permeabilized fibers, OXPHOS capacity, Coupling control, Fatty acid oxidation

...(succinate&rotenone) restricts ET-capacity and artificially enhances flux control upstream of the Q-cycle, providing diagnostic information on specific branc ...e experimental basis for high accuracy of quantitative results and quality control in high-resolution respirometry.

...´ and ''E''´. When expressing ''Rox'' as a fraction of ET capacity ([[flux control ratio]]), total flux ''E''´ (not corrected for ''Rox''), should be taken a == Keywords ==

...te4) was calculated as an index of mitochondrial oxidative-phosphorylation coupling. Our results show that the activity of the mitochondrial complex I in the b |keywords=Brain development, Brainstem, Mice, Mitochondria, Sex dimorphism, Cortex

...compared with CG. Mitochondrial respiratory capacity, biogenesis, and the coupling between oxidative phosphorylation and ATP synthesis were significantly decr |keywords=Bone marrow cells, High-fat diet, Mitochondria, Obesity, Oxidative phosphor

...cells grown in the presence of IL-3. This was consistent with respiratory control exerted by impaired Complex IV activity, since there was not even the sligh |keywords=Mitochondrial respiration, OXPHOS, cytochrome c oxidase, apoptosis, CRAF, i

...ine (ADMA) and NG-monomethyl-L-arginine, in tumor-bearing mice compared to control mice. Compared to healthy controls, human cancer patients were also found t |keywords=Cachexia, Cancer, Metabolomics, Methylarginines, Mitochondria, Protein synt

...ence that early progression of hyperglycemia, in brain tissue, changes the coupling of glucose phosphorylation at the level of mitochondria by rearranging the |keywords=Diabetes Mellitus type 1, NF-kB, PTEN, Mitochondrial ROS, Mitochondrial bra

...fects mitochondrial respiration by causing a reduction in both respiratory control ratio (IC(50) = 200 ± 15 nM) and [[State 3]] respiration (IC(50) = 192 ± |keywords=Parkinson's disease, 6-hydroxydopamine (6-OHDA)

...to low salinity increased mitochondrial oxidative phosphorylation rate and coupling efficiency and stimulated mitochondrial respiration after acute H/R stress. |keywords=Aerobic capacity, AmplexRed, High-resolution respirometry, Hypoxia toleranc

...come established world-wide for the diagnosis of mitochondrial respiratory control and OXPHOS analysis, reflected in [[O2k-Publications|>1,000 O2k-publication ...heart mitochondria]] was not only a function of respiratory substrate and coupling state, but depended strongly on experimental oxygen levels below and even a

....26124/bec:2020-0002 - '''Chapter 8: Protonmotive pressure and respiratory control''' [[File:Hydrogen ion circuit.jpg|right|330px|thumb|Figure 1.1. Coupling in oxidative phosphorylation is mediated by the protonmotive force ''pmF''.

...eased leak respiration and decreased phosphorylation efficiency and OXPHOS coupling efficiency in both species, which may serve to augment non-shivering thermo ...-altitude adaptation, Lipid oxidation, Mitochondrial metabolism, Substrate control ratio

|keywords=pulmonary hypertension(ph), chronic hypoxia, pulmonary vascular remodeling, |topics=ADP, Calcium, Coupling efficiency;uncoupling, Flux control, Inhibitor, mt-Membrane potential, Substrate, Uncoupler

...ibiting myosin-ATPase reveals a dynamic range of mitochondrial respiratory control in skeletal muscle. Biochem J 437:215-22. ...actile state and cellular energy charge, providing evidence that enzymatic coupling of energy transfer within skeletal muscle becomes more efficient in the wor

...in animals receiving colloids (p<0.05). CS activity and electron transfer coupling efficiency were significantly lower in burned animals, particularly with co |keywords=Burn, Heart, Mitochondria, Resuscitation, Swine

|title=Scott GR, Richards JG, Milsom WK (2009) Control of respiration in flight muscle from the high-altitude bar-headed goose and ...lation in flight muscle of bar-headed geese, which could promote efficient coupling of ATP supply and demand. However, this was not based on differences in cre

...suspension of fibroblasts did not affect respiratory activity and coupling control. Evaluation of results obtained by different platforms provides a test for |keywords=attached cells; ace, suspended cells; sce, fibroblasts, high-resolution res

...ink M, Calbet JAL, Christiansen M, Helge JW, Saltin B (2015) Mitochondrial coupling and capacity of oxidative phosphorylation in skeletal muscle of Inuit and c ...here economy of locomotion may be optimized by preservation of biochemical coupling efficiency at modest mitochondrial density, when submaximum performance is

|title=Gnaiger E (1989) Mitochondrial respiratory control: energetics, kinetics and efficiency. In: Energy transformations in cells a ...isms of energy transformation, emphasizing the effect of efficiency on the control of flux.

...wer capacity of carbohydrates and fatty acids oxidation and also decreased coupling between oxidative phosphorylation and ATP synthesis. Cardiac metabolism imp |keywords=Diet-induced obesity, Mitochondrial bioenergetics, Mitochondrial biogenesis

...weight change and muscle fiber size (R2=0.73-0.76, P<0.01). Mitochondrial coupling efficiency, which is compromised in cachexic livers to potentially increase |keywords=STRING, TMT, Bioinformatics, Mitochondrial function, Quantitative proteomic

| style="text-align:left"|[[O2k-Publications: Coupling efficiency;uncoupling|Coupling efficiency;uncoupling]] ...:[[Category:Publications]] [[Instrument and method::Oxygraph-2k]] [[Topic::Coupling efficiency;uncoupling]] |format=count}}

...incubation. Remifentanil increased the isolated mitochondrial respiratory control ratio of complex-I-dependent respiration without interfering with maximal r |keywords=Sepsis, Liver dysfunction, Remifentanil, Tumor necrosis factor-α (TNF-α),

...adriceps (vastus lateralis) with respect to their respiratory capacity and control. |keywords=Comparative Physiology

...of PDH and improved mitochondrial coupling as expressed by the respiratory control ratio (RCR). Notably, DCA completely prevented the toxicity of SOD1(G93A) a |keywords=Amyotrophic lateral sclerosis, Astrocyte cultures, SOD1(G93A) mice, Dichlor

...ft|90px|Erich Gnaiger]] The protonmotive force and respiratory control. 1. Coupling of electron transfer reactions to vectorial translocation of protons. 2. Fr ...n transfer (ET) are coupled to vectorial translocation of protons at three coupling sites, which are the proton pumps of the ET system (ETS): respiratory Compl

...physical exercise. Cause and effect are embedded in a network of feedback control and circular loops in degenerative diseases. |keywords=Preventive mitochondrial medicine, BMI, mt-density, OXPHOS capacity, diabes

...r-related respiration were significantly lower in the MD compared with the control group. Furthermore, mitochondrial respiration was significantly negatively |keywords=PBMC

...alking bout. Fasted muscle biopsies were collected on day 5. Mitochondrial coupling in the presence of lipid-associated substrates was higher after ONE (4.8 ± |keywords=Active breaks, mRNA sequencing, Mitochondria, Obesity, Sedentary behavior,

...e-linked pathways in the LEAK, OXPHOS, and electron transfer (ET) coupling control states. We observed that the Q-pool became partially reduced upon addition ...LHD, Doerrier C, Moore AL, Rich PR, Gnaiger E (2021) Coupling and pathway control of coenzyme Q redox state and respiration in isolated mitochondria. https:/

...by relationships derived between ~P/O₂ flux ratios, respiratory control ratios, and ADP kinetics. Bioenergetics at low oxygen versus oxidative stre |keywords=Energy: Oxidative phosphorylation, Adenosine diphosphate kinetics, Adenosin

...espiratory control ratio (RCR), an indicator of the state of mitochondrial coupling, which is related to the mitochondrial functionality. In addition, mitochon |keywords=High-resolution respirometry, Mitochondrial functionality, Nucleoside, Weig

...acterized in tissue homogenates by assessing the succinate-linked coupling control. Mitochondrial parameters were then correlated with clinical outcome (“ea ...r-the-curve (AUC) values of LEAK respiration, cytochrome ''c'' and ''P-L'' control efficiencies during the first 6 h of NMP correlated with L-GrAFT.

...profile (maximal oxygen consumption rate, coupling efficiency, respiratory control ratio and spare respiratory capacity) was aberrant in cultured sensory neur |keywords=Streptozotocin-diabetic rats, db/db mice, Type 1 and type 2 diabetes, Dorsa

...rgogenic effects of BRJ ingestion are not due to a change in mitochondrial coupling or efficiency. It remains to be determined if increased mitochondrial H<sub |keywords=Blebbistatin, Buffer z, Amplex Red in muscle fibers, Safranin

...ncrease in treadmill endurance compared to both pretreatment and untreated control values. These results demonstrate that the shift of redox homeostasis due t |keywords=Aging, Fatigue, Mitochondria, Oxidative stress, Skeletal muscle

:::::Keywords: MIA, PA M, SAM, TIM, TOM. :::::Keywords: apoptosis, necrosis, cytochrome c, mitochondrial membrane, permeability tr

...ry of mitochondrial preparations can assess electron transfer pathways and coupling in oxidative metabolism with high diagnostic resolution [1].<br> # Gnaiger E (2020) Mitochondrial pathways and respiratory control. An introduction to OXPHOS analysis. 5th ed. https://doi.org/10.26124/bec:2

...ity from mouse to man, and evaluation of altered mitochondrial respiratory control patterns in health and disease. |keywords=[[Q-junction]], Q-cycle, Pyruvate, Glutamate, Succinate, Tricarboxylic acid

...ntly highest in the succinate-linked LEAK state, reduced with [[NS-pathway control state |NS-linked convergent electron flow]] and in mitochondria respiring a |keywords=High-resolution respirometry, H₂O₂ flux, Amplex Red,

...ctron transport capacity between patients with type 2 diabetes and healthy control subjects. |keywords=Diabetes, Mitochondria, Skeletal muscle

...suspension of fibroblasts did not affect respiratory activity and coupling control. Consistent results obtained with different platforms provide a test for re |keywords=attached cells; ace, suspended cells; sce, fibroblasts, high-resolution res

...upled]]) and correspondingly lower [[OXPHOS control ratio|''P/E'' coupling control ratios]]. The inhibition is eliminated as the diapause extract is diluted, |keywords=[[Diapause]], Metabolic inhibition, ''Artemia franciscana'', [[Complex I]],

...Pathways|comprehensive OXPHOS analysis]] of substrate control and coupling control of mitochondrial function. 'High throughput' stands for disposable multiwel ::: <span style="font-size:105%; color:##424242">'''» Keywords'''</span>

...omplex I substrates, acutely lowering NEFA after permeabilization improved coupling but only minimally increased S3. In contrast, lowering NEFA during 60 min. |keywords=Acute Kidney Injury, Hypoxia/Reoxygenation, Isolated Permeabilized Renal Tu

...rotocols]], [[Export DL-Protocol User (*.DLPU)|DL-Protocol User]], quality control, data analysis, documentation, and traceability of measurements with the [[ |keywords= DatLab, [[MiPNet22.16 DatLab 7 Innovations: DL-Protocols|Innovation DL-Pro

...als in muscle fibers: a fast signal associated with excitation-contraction coupling (ECC) and a slow signal that is preferentially formed in the nucleus and re ...words=Central core disease, Multi minicore disease, Excitation-contraction coupling

...₂O₂ production were performed in the LEAK and OXPHOS coupling states fueled by the NADH-linked substrates pyruvate & malate. Upon transit ...Fontana-Ayoub M, Gnaiger E (2015) Cytochrome redox states and respiratory control in mouse and beef heart mitochondria at steady-state levels of hypoxia. htt

...onium (TPP⁺) concentration 1 and 1.5 µM. Coupling and substrate control states [1] were established in 6 different [[SUIT]] protocols. In calculati |keywords=ΔΨ, Mitochondrial preparations, [[SUIT]] protocols

...Gnaiger E (2021) Bioenergetic cluster analysis – mitochondrial respiratory control in human fibroblasts. MitoFit Preprints 2021.08. https://doi.org/10.26124/m ..._MitoFit_BCA.pdf Bioenergetic cluster analysis – mitochondrial respiratory control in human fibroblasts]

|keywords=Glutamate dehydrogenase ...0 s). This is in line with a ''[[P/E]]'' ratio of ~0.9 and normal coupling control of mitochondrial respiration. Although the angiography did not indicate any

...combination allows operation with programmable titration regimes, feedback control, and at steady-states, yielding a new flexibility in experimental design by {{Template:Keywords: O2k-technology}}

::: <span style="font-size:105%; color:##424242">'''» Keywords'''</span> [[Category:Coupling control]]

...endence of mitochondrial respiration as a function of pathway and coupling control in liver, heart and brain ...endence of mitochondrial respiration as a function of pathway and coupling control in liver, heart and brain

...he Titration-Injection-microPump [[TIP2k]] can be programmed for automatic control of titration steps in a DL-Protocol. In DatLab 7.4, it is possible to edit == Keywords: Oxygen signal ==

Gnaiger E (2012) Mitochondrial pathways and respiratory control. An introduction to OXPHOS analysis. 3rd ed. Mitochondr Physiol Network 17. |keywords=Archive

...tics. We endeavour to provide a balanced view on mitochondrial respiratory control and a critical discussion on reporting data of mitochondrial respiration in |keywords=Mitochondrial respiratory control, coupling control, mitochondrial preparations, protonmotive force, uncoupling, oxidative phos

{{Template:Keywords: Charge}} :::# Gnaiger E (2020) Mitochondrial pathways and respiratory control. An introduction to OXPHOS analysis. 5th ed. Bioenerg Commun 2020.2. - [[Gn

::: <span style="font-size:105%; color:##424242">'''» Keywords'''</span> ...ry flux by non-saturating substrate concentration can be excluded. Kinetic control studies, therefore, provide the basis of critically examining the appropria

...et/dry mass ratios applicable to studies reporting dry mass only; (2) flux control ratios applied to calculate combined NADH- and succinate-linked OXPHOS capa ...nce body mass determined by your height. The consequences of mitochondrial control on ''V''_{O₂max/''M''} will be discussed in terms of mec

...cated in the pathogenesis of autism. We compared mitochondrial respiratory control in autism with normal lymphoblastoid cell line and found that the oxidative |keywords=autism spectrum disorders, oxidative phosphorylation, cytochrome ''c'' oxid

Gnaiger E (2014) Mitochondrial pathways and respiratory control. An introduction to OXPHOS analysis. 4^th ed. Mitochondr Physiol ...tates |Respiratory states]], [[MitoPedia: Respiratory control ratios |Flux control ratios]]

|keywords=MiP ::::*Respiratory control in isolated mitochondria and permeabilized cells and fibers.

|title=Gnaiger E (2020) Mitochondrial pathways and respiratory control. An introduction to OXPHOS analysis. 5^th ed. Bioenerg Commun 202 ...ance to society. The ‘Blue Book’ on Mitochondrial Pathways and Respiratory Control presents a fundamental introduction to OXPHOS analysis for students and res

...tics. We endeavour to provide a balanced view on mitochondrial respiratory control and a critical discussion on reporting data of mitochondrial respiration in |keywords=Mitochondrial respiratory control, coupling control, mitochondrial preparations, protonmotive force, uncoupling, oxidative phos

::::# Set the polarisation voltage of the O2 sensor to 0 V in the O2k Control window. Observe any effects on the pX raw signal. A tiny potential jump i ...nd '''Offset voltage [mV]''' for the pX channel can be set in the '''[[O2k control]] window''' [F7], tab: '''Potentiometric, pX'''. The gain influences the "

|keywords=Isolated mitochondria, Permeabilized myofibers, ROS ...ereas the RCR>9 in imt comes closer to ''in vivo'' values. The low ''P-L'' control efficiency in permeabilized fibers is an unresolved puzzle, and confirmatio

...tics. We endeavour to provide a balanced view of mitochondrial respiratory control and a critical discussion on reporting data of mitochondrial respiration in == Keywords—[[MitoPedia]] ==

...ng Complex II in the literature and educational resources call for quality control, to secure scientific standards in current communications of bioenergetics, |keywords=[[coenzyme]]; [[cofactor]]; [[prosthetic group]]; coenzyme Q junction, Q-ju