Christian 2019 Respir Physiol Neurobiol
|Christian AR, Losantos-Ramos K, Marcelino G, Daniela F, Jorge S (2019) NADH-linked mitochondrial respiration in the developing mouse brain is sex-, age- and tissue-dependent. Respir Physiol Neurobiol 266:156-62.|
Abstract: Mitochondria play a major role in the brain. Apart from energy production, mitochondria regulate key factors in the activation of cell signaling pathways such as survival, proliferation, and differentiation. While all these processes occur during the physiological development of the brain, it is surprising that the mitochondrial functions and functioning in the brain during the postnatal development remain poorly explored. In this work, we collected samples of brainstem and cortex of mice at postnatal ages 3 (P3), 21 (P21), and at adulthood (3 months old) and evaluated the mitochondrial oxygen consumption after complex I activation. To do so, we used our Oxygraph-2k system (OROBOROS Instruments, Austria) that measures the mitochondrial bioenergetics in saponin-permeabilized tissue punches of 2 mg weight. Furthermore, as sex dimorphism in the brain occurs since very early stages of development, we performed experiments in brain samples of male and female mice. Accordingly, the mitochondrial oxygen consumption rate (OCR) was evaluated under activation of complex I (NADH-linked respiration - mitochondrial state 3), and during the inhibition of the complex V (ATP synthase) with oligomycin (mitochondrial state 4). In following, the respiratory control ratio (RCR - state 3/state4) was calculated as an index of mitochondrial oxidative-phosphorylation coupling. Our results show that the activity of the mitochondrial complex I in the brain increases along with the postnatal development in a sex- and tissue-dependent manner, with males showing higher activity than females, and with brainstem tissue showing higher activity than cortex. Our data may contribute to a better understanding of the sex-dependent maturation of the cortex and the cardiorespiratory network located in the brainstem.
Copyright © 2019. Published by Elsevier B.V. • Keywords: Brain development, Brainstem, Mice, Mitochondria, Sex dimorphism, Cortex • Bioblast editor: Plangger M
Labels: MiParea: Respiration, Gender, Developmental biology
Organism: Mouse Tissue;cell: Nervous system Preparation: Permeabilized tissue
Coupling state: LEAK, OXPHOS Pathway: N HRR: Oxygraph-2k