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De Jong 2022 Nutrients

From Bioblast
Publications in the MiPMap
De Jong NP, Rudolph MC, Jackman MR, Sharp RR, Jones K, Houck J, Pan Z, Reusch JEB, MacLean PS, Bessesen DH, Bergouignan A (2022) Short-term adaptations in skeletal muscle mitochondrial oxidative capacity and metabolic pathways to breaking up sedentary behaviors in overweight or obese adults.

» Nutrients 14:454. PMID: 35276813 Open Access

De Jong Nathan P,  Rudolph Michael C, Jackman Matthew R,  Sharp Rachel R,  Jones Ken,  Houck Julie,  Pan Zhaoxing,  Reusch Jane EB,  MacLean Paul S,  Bessesen Daniel H,  Bergouignan Audrey (2022) Nutrients

Abstract: Breaking up sedentary behavior with short-frequent bouts of physical activity (PA) differentially influences metabolic health compared with the performance of a single-continuous bout of PA matched for total active time. However, the underlying mechanisms are unknown. We compared skeletal muscle mitochondrial respiration (high-resolution respirometry) and molecular adaptations (RNA sequencing) following 4-day exposure to breaks vs. energy-matched single-continuous PA bout in inactive adults with overweight/obesity. Participants (9M/10F, 32.2 ± 6.4 years, 30.3 ± 3.0 kg/m2) completed three 4-day interventions of a randomized cross-over study: SED, sedentary control; MICRO, 5 min brisk walking each hour for 9 h; ONE: 45 min/d continuous brisk walking bout. Fasted muscle biopsies were collected on day 5. Mitochondrial coupling in the presence of lipid-associated substrates was higher after ONE (4.8 ± 2.5) compared to MICRO (3.1 ± 1.1, p = 0.02) and SED (2.3 ± 1.0, p = 0.001). Respiratory rates did not differ across groups with carbohydrate-associated substrates. In pathways associated with muscle contraction transcription signaling, ONE and MICRO similarly enhanced Oxidative Phosphorylation and Sirtuin Signaling expression (p < 0.0001, for both). However, ONE (p < 0.001, for all), but not MICRO, had greater pathway enrichment, including Ca++, mTOR, AMPK, and HIF1α signaling, than SED. Although breaking up sedentary behavior triggered skeletal muscle molecular adaptations favoring oxidative capacity, it did not improve mitochondrial function over the short term. Keywords: Active breaks, mRNA sequencing, Mitochondria, Obesity, Sedentary behavior, Skeletal muscle molecular adaptations Bioblast editor: Plangger M O2k-Network Lab: US CO Aurora Jackman MR

Labels: MiParea: Respiration, Exercise physiology;nutrition;life style  Pathology: Obesity 

Organism: Human  Tissue;cell: Skeletal muscle  Preparation: Permeabilized tissue 

Coupling state: LEAK, OXPHOS, ET  Pathway: F, N, S, NS, ROX  HRR: Oxygraph-2k