SUIT-020: Difference between revisions
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{{MitoPedia | {{MitoPedia | ||
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|description=[[File: | |description=[[File:|200px]] | ||
|info='''A:'''[[File:PDF.jpg|100px|link=http://wiki.oroboros.at/images/6/64/SUIT-020.pdf|Bioblast pdf]] ยป[http://wiki.oroboros.at/index.php/File:SUIT-020.pdf Versions] | |info='''A:'''[[File:PDF.jpg|100px|link=http://wiki.oroboros.at/images/6/64/SUIT-020.pdf|Bioblast pdf]] ยป[http://wiki.oroboros.at/index.php/File:SUIT-020.pdf Versions] | ||
}} | }} | ||
::: '''[[Categories of SUIT protocols |SUIT-category]]:''' | ::: '''[[Categories of SUIT protocols |SUIT-category]]:''' | ||
::: '''[[SUIT protocol pattern]]:''' | ::: '''[[SUIT protocol pattern]]:''' | ||
__TOC__ | __TOC__ | ||
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== Strengths and limitations == | == Strengths and limitations == | ||
== Compare SUIT protocols == | == Compare SUIT protocols == |
Revision as of 15:29, 18 January 2019
Description
[[File:|200px]]
Reference: A: ยปVersions
DLP applications
References
Year | Reference | Organism | Tissue;cell | |
---|---|---|---|---|
MiPNet20.13 Safranin mt-membranepotential | 2019-06-24 | O2k-FluoRespirometry: HRR and simultaneous determination of mt-membrane potential with safranin or TMRM. | Mouse | Nervous system |
Krumschnabel 2014 Methods Enzymol | 2014 | Krumschnabel G, Eigentler A, Fasching M, Gnaiger E (2014) Use of safranin for the assessment of mitochondrial membrane potential by high-resolution respirometry and fluorometry. Methods Enzymol 542:163-81. https://doi.org/10.1016/B978-0-12-416618-9.00009-1 | Mouse | Nervous system |
Steps and respiratory states
Step | State | Pathway | Q-junction | Comment - Events (E) and Marks (M) |
---|---|---|---|---|
1PM | PML(n) | N | CI | 1PM
|
2D | PMP | N | CI | 1PM;2D
|
2c | PMcP | N | CI | 1PM;2D;2c
|
3G | PGMP | N | CI | 1PM;2D;2c;3G
|
4S | PGMSP | NS | CI&II | 1PM;2D;2c;3G;4S
|
5Rot | SP | S | CII | 1PM;2D;2c;3G;4S;5Rot
|
6Omy | SL(Omy) | 1PM;2D;2c;3G;4S;5Rot;6Omy
| ||
7U* | SE | S | CII | 1PM;2D;2c;3G;4S;5Rot;6Omy;7S
|
8Ama | ROX | 1PM;2D;2c;3G;4S;5Rot;6Omy;7S;8Ama
|
Step | Respiratory state | Pathway control | ET-Complex | Comment |
---|---|---|---|---|
## AsTm | AsTmE | CIV | CIV | |
## Azd | CHB |
- Bioblast links: SUIT protocols - >>>>>>> - Click on [Expand] or [Collapse] - >>>>>>>
- Coupling control
- Pathway control
- ยป Electron transfer pathway
- ยป Fatty acid oxidation pathway control state, F
- ยป NADH electron transfer-pathway state, N
- ยป Succinate pathway control state, S
- ยป NS-pathway control state, NS
- ยป Glycerophosphate pathway control state, Gp
- ยป Complex IV single step, CIV
- ยป Anaplerotic pathway control state
- Pathway control
- Main fuel substrates
- ยป Glutamate, G
- ยป Glycerophosphate, Gp
- ยป Malate, M
- ยป Octanoylcarnitine, Oct
- ยป Pyruvate, P
- ยป Succinate, S
- Main fuel substrates
- Glossary
Strengths and limitations
Compare SUIT protocols
MitoPedia concepts: MiP concept, SUIT protocol, Recommended
MitoPedia methods:
Respirometry