OROBOROS INSTRUMENTS

Bioblast Agenda - 2024 week 50

Oroboros declares solidarity against the Russian aggression-war in Ukraine and against all aggression-wars worldwide. - »More details

-» A future for Ukraine is the future of its science

2024 Week 50

Dec-09 Mo

Metal chelation decreases the high background flux in the Amplex® UltraRed assay

DTPA (Diethylenetriaminepentaacetic acid) is a metal chelator that can be incorporated into the Amplex® UltraRed assay to decrease the high background fluxes associated with iron contamination.
- »DTPA«; »Amplex UltraRed«
- communicated by »Willis Jaime R« and »Grings Mateus«

Dec-10 Tu

Dec-11 We

Dec-12 Th

"When working with potentially sticky tissue, stop the stirrer, siphon off H₂O, remove the stirrer (...) clean the stirrer bar with a paper towel and rinse it with H₂O. Return to the same chamber."

- »MiPNet19.03 O2k-cleaning and ISS«
- communicated by »Cardoso Luiza« and »Cecatto Cristiane«

Dec-13 Fr

Next Week

2024 Week 51

Dec-16 Mo

“Thank you for your detailed and informative response—I truly appreciate your guidance.”

Kazi Sarjana Safain, PhD Candidate from the O2k-Network »US ND Fargo Swanson K«
- »Oroboros Feedback«

Dec-17 Tu

Dec-18 We

Dec-19 Th

Quality control by precision OXPHOS analysis indicates reproducible results with all lots of MiR05-Kit and storage times up to 4 years

Baglivo E, Cardoso LHD, Cecatto C, Gnaiger E (2024) Stability of mitochondrial respiration medium used in high-resolution respirometry with living and permeabilized cells. Bioenerg Commun 2024.8 »Bioblast link«

from the O2k-Network »AT Innsbruck Oroboros«

Dec-20 Fr

In focus

2025 Oroboros Workshops:
Oroboros Coaching Days - Advanced - Fluo IOC171
2025 Feb 18-20
Innsbruck, Austria
Oroboros Workshop on high-resolution respirometry
2025 Jun 23 - 28
Schroecken, Austria
Oroboros Workshop on high-resolution respirometry
2025 Sep 29 - Oct 04
Schroecken, Austria

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Oroboros of the month

In the artwork "First Fruits of the Earth Offered to Saturn" at the Palazzo Vecchio, Saturn (Cronus in Greek mythology) is holding the Ouroboros in the hand, symbolizing the eternal cycle of renewal and the interconnectedness of beginning and end.

» Oroboros pictures of the month

Bioblast: About

Bioblast is an open access platform, including MitoPedia as a glossary and index for mitochondrial and chloroplast physiology, hosting MitoGlobal, and supporting the journal Bioenergetics Communications and the Oroboros Ecosystem.
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2024 Week 52

Dec-23 Mo

How can titration artifacts in the flux plot be hidden to have a more clear figure of the trace?

Select the corresponding plot in one of the graphs, press Ctrl+Shift+left click at the chosen starting time point and drag the cursor over the section to be hidden.
- »Hide/Show points - DatLab«
- communicated by »Grings Mateus« and »Baglivo Eleonora«

Dec-24 Tu

Dec-25 We

Dec-26 Th

"When using a syringe with a chemical different from the one usually assigned to the syringe, repeated washing steps and leaving the syringe filled with absolute ethanol overnight may be crucial to prevent contamination by carry-over."

- »MiPNet19.14 SOP Hamilton microsyringes«
- communicated by »Baglivo Eleonora« and »Cecatto Cristiane«

Dec-27 Fr

2025 Week 01

Dec-30 Mo

Why use a Cover-Slip?

Placing a Cover-Slip on the Stopper minimizes contamination and evaporation. During optical measurements in the Oroboros, using the black Cover-Slip prevents light penetration and disturbance of the fluorescence signal.
- »Cover-Slip/black«
- communicated by »Leo Elettra« and »Cecatto Cristiane«

Dec-31 Tu

Jan-1 We

Jan-2 Th

Use the correct DatLab layout for your experiment

The layouts flux per volume and specific flux show instrumental background corrected O₂ fluxes.
- »Layout - DatLab«
- communicated by »Cecatto Cristiane« and »Grings Mateus«

Jan-3 Fr

2025 Week 2

Jan-6 Mo

How can the uncoupler concentration affect my assay?

To measure ET capacity, uncouplers such as CCCP or FCCP must be titrated stepwise to reach optimum concentration, thus avoiding inhibition of respiration at higher concentrations.
- »Uncoupler«, »ET capacity«
- communicated by »Cecatto Cristiane« and »Leo Elettra«

Jan-7 Tu

Jan-8 We

Jan-9 Th

Jan-10 Fr

2025 Week 3

Jan-13 Mo

Jan-14 Tu

Jan-15 We

Jan-16 Th

Jan-17 Fr

2025 Week 4

Jan-20 Mo

Jan-21 Tu

Jan-22 We

Jan-23 Th

"Assessment of ATP dynamics in real-time showed that the mitochondrial capacity to produce ATP was diminished at elevated temperatures as ATP hydrolysis rates increased."

Willis JR, Hickey AJR, Devaux JBL (2021) Thermally tolerant intertidal triplefin fish (Tripterygiidae) sustain ATP dynamics better than subtidal species under acute heat stress. Sci Rep 11:11074. »Bioblast link«

from the O2k-Network »NZ Auckland Hickey AJ«

Jan-24 Fr

2025 Week 5

Jan-27 Mo

Jan-28 Tu

Jan-29 We

Jan-30 Th

Jan-31 Fr

Last week and before

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