Oroboros Ecosystem Agenda 2023 Archive

Oroboros Ecosystem Agenda 2023 Archive

Mitochondria and Cell Research - the Oroboros Ecosystem

Dec 2023

2023 Week 49

Dec- 04 Mo

Dec- 05 Tu

Is DatLab 8 compatible with your operative system?

The DatLab 8 software is designed for 64-bit versions of Windows operating systems and does not run on MAC devices.
- »DatLab 8«
- communicated by »Leo Elettra« and »Baglivo Eleonora«

Dec- 06 We

"The capacity of FA oxidative phosphorylation (F-OXPHOS) with palmitoylcarnitine was up to 4 times higher than respiration with octanoylcarnitine. The optimal concentration of palmitoylcarnitine was 10 μM which corresponds to the total concentration of LC acylcarnitines in the brain."

Cecatto C, Cardoso LHD, Ozola M, Korzh S, Zvejniece L, Gukalova B, Doerrier C, Dambrova M, Gnaiger E, Makrecka-Kuka M, Liepinsh E (2023) Fatty acid β-oxidation in brain mitochondria: Insights from high-resolution respirometry in mouse, rat and Drosophila brain, ischemia and aging models. MitoFit Preprints 2023.10. https://doi.org/10.26124/mitofit:2023-0010

from the O2k-Network »AT Innsbruck Oroboros« and »LV Riga Liepins E«

Dec- 07 Th

"(…) L-Erg [L-Ergothioneine] treatment prevented and delayed cystine lithiasis in a cystinuria mouse model by increasing urinary cystine solubility and restored kidney GSH content and OXPHOS function."

Mayayo-Vallverdú C, López de Heredia M, Prat E, González L, Espino Guarch M, Vilches C, Muñoz L, Asensi MA, Serra C, Llebaria A, Casado M, Artuch R, Garrabou G, Garcia-Roves PM, Pallardó FV, Nunes V (2023) The antioxidant l-Ergothioneine prevents cystine lithiasis in the Slc7a9-/- mouse model of cystinuria »Bioblast link«

from the O2k-Network »ES Barcelona Moren C« and »ES Barcelona Garcia-Roves PM«

Dec- 08 Fr

2023 Week 50

Dec- 11 Mo - International Mountain Day

Mountain slopes can be difficult, but O₂ slopes can be smooth with DatLab

The default setting of Slope smoothing is 40 data points, corresponding to an interval of 80 s at a data recording interval of 2 s. DatLab 7 expresses it in data points and DatLab 8 in seconds.
- »Smoothing in Flux/Slope«
- communicated by »Timon-Gomez Alba« and »Baglivo Eleonora«

Dec- 12 Tu

From preprint to Gold Open Access publication in J Biol Chem

Gnaiger E (2023) Complex II ambiguities ― FADH2 in the electron transfer system. »Bioblast link«

Dec- 13 We

Dec- 14 Th

"HCR [High capacity runner rats] showed a consistent and robust trend of higher maximal oxygen consumption compared to LCR [low capacity runner rats], especially for long chain FAs [fatty acids]."

Fleischman JY, Van den Bergh F, Collins NL, Bowers M, Beard DA, Burant CF (2023) Higher mitochondrial oxidative capacity is the primary molecular differentiator in muscle of rats with high and low intrinsic cardiorespiratory fitness. »Bioblast link«

from the O2k-Network »US MI Ann Arbor Beard DA«

Dec- 15 Fr

2023 Week 51

Dec- 18 Mo

»SBC 2023 Goa IN« Dec 18-20

Why add azide after ascorbate and TMPD when measuring Complex IV activity?

The O₂ flux measured after the titration of ascorbate and TMPD is due to both CIV activity and autooxidation of these compounds. By subsequently inhibiting CIV activity with azide, the O₂ flux due to autooxidation (chemical background) can be determined and subtracted from the initial measurement.
- »Complex IV«, »Chemical background «
- communicated by »Grings Mateus« and »Timon-Gomez Alba«

Dec- 19 Tu

Dec- 20 We

"SIRT4 [Sirtuin 4] knockdown increases mitochondrial capacity to oxidize fatty acids, decreasing LEAK respiration and further increasing mitochondrial elongation via its regulation of mitochondrial fusion."

Noone J, Rochfort KD, O'Sullivan F, O'Gorman DJ (2023) SIRT4 is a regulator of human skeletal muscle fatty acid metabolism influencing inner and outer mitochondrial membrane-mediated fusion. »Bioblast link«

from the O2k-Network »US FL Orlando Goodpaster BH« and »IE Dublin O Gorman D«

Dec- 21 Th

Dec- 22 Fr

2023 Week 52

Dec- 25 Mo Our office and technical support will be closed for the Christmas period until January 7, 2024.

Dec- 26 Tu

Dec- 27 We

Do you observe a high signal at zero oxygen?

If the quality of your dithionite solution has already been checked, perform a leak current test to evaluate which electrical component is generating the out-of-scale signal.
- »Leak current test«
- communicated by »Baglivo Eleonora« and »Grings Mateus«

Dec- 28 Th

Dec- 29 Fr

"Leptin receptor signaling in Schwann cells adjusts catabolic processes, including mitochondrial respiration and autophagy, facilitating nerve repair."

Sundaram VK, Schütza V, Schröter NH, Backhaus A, Bilsing A, Joneck L, Seelbach A, Mutschler C, Gomez-Sanchez JA, Schäffner E, Sánchez EE, Akkermann D, Paul C, Schwagarus N, Müller S, Odle A, Childs G, Ewers D, Kungl T, Sitte M, Salinas G, Sereda MW, Nave KA, Schwab MH, Ost M, Arthur-Farraj P, Stassart RM, Fledrich R (2023) Adipo-glial signaling mediates metabolic adaptation in peripheral nerve regeneration. »Bioblast link«

from the O2k-Network »DE_Leipzip_Ost_M«

Nov 2023

2023 Week 44

Nov- 01 We

Nov- 02 Th

Nov- 03 Fr

"Peripheral impairments at the skeletal muscle level, namely reduced mitochondrial function and the presence of markers of impaired mitochondrial biogenesis emerged as major determinants of exercise intolerance and fatigue [in patients with post-acute sequelae of SARS-CoV-2 infection]"

Colosio M, Brocca L, Gatti M, Neri M, Crea E, Cadile F, Canepari M, Pellegrino MA, Polla B, Porcelli S, Bottinelli R (2023) Structural and functional impairments of skeletal muscle in patients with post-acute sequelae of SARS-CoV-2 infection. »Bioblast link«

2023 Week 45

Nov- 06 Mo

Nov- 07 Tu

How often should I re-assemble the O2k-chamber?

We recommend disassembling, cleaning and re-assembling the O2k-chamber at least every 1-2 years. It is also necessary to disassemble it when there is persistent biological or protein contamination, medium leakage from the chamber, an oxygen leak of the chamber, or an unstable or unexpectedly high or low oxygen signal.
- »O2k-chamber assembly - possible problems« and »O2k-Videosupport: Insert O2k-chamber«
- communicated by »Timon-Gomez Alba« and »Schmitt Sabine«

Nov- 08 We

Nov- 09 Th

Nov- 10 Fr

When should I analyze my data as flux control ratios (FCRs)?

FCRs enable the comparison of samples normalized with various mitochondrial content parameters or when sample concentration is unknown. This is achieved by internally normalizing O₂ fluxes in different respiratory states to the maximum flux in a common reference state.
- »Flux control ratio«
- communicated by »Baglivo Eleonora« and »Timon-Gomez Alba«

2023 Week 46

Nov- 13 Mo

Nov- 14 Tu - World Diabetes Day

"Liver [mitochondria] (...) adapt to metabolic challenges by increasing FAO-derived mitochondrial respiration in OBES [obesity model] and NASH [non-alcoholic steatohepatitis model], but by increasing both FAO- and TCA-derived respiration in DIAB [diabetes model]."

Dewidar B, Mastrototaro L, Englisch C, Ress C, Granata C, Rohbeck E, Pesta D, Heilmann G, Wolkersdorfer M, Esposito I, Reina Do Fundo M, Zivehe F, Yavas A, Roden M (2023) Alterations of hepatic energy metabolism in murine models of obesity, diabetes and fatty liver diseases. »Bioblast link«

from the O2k-Network »DE Duesseldorf Roden M« and »DE Cologne Pesta D«

Nov- 15 We

Nov- 16 Th - World Pancreatic Cancer Day

"To the advantage of PDAC [pancreatic ductal adenocarcinoma] patients, innovative and more effective therapeutics will be possible with a greater understanding of how pancreatic cancer cells control mitochondrial metabolism."

Padinharayil H, Rai V, George A (2023) Mitochondrial metabolism in pancreatic ductal adenocarcinoma: from mechanism-based perspectives to therapy. »Bioblast link«

Nov- 17 Fr

2023 Week 47

Nov- 20 Mo

Do I need to change the filter set of the O2k-Fluo Smart-Module?

Because two different filter sets can be used with the blue Fluo-Sensors, filters should be changed according to the fluorophore that will be used. Extra filters are provided for replacement in case of damage.
- »O2k-Fluo Smart-Module manual« and »O2k-Fluo Smart-Module - Fluorescence dyes and settings«
- communicated by »Cardoso Luiza« and »Timon-Gomez Alba«

Nov- 21 Tu

Nov- 22 We

“The cautionary note provided by these studies emphasizes the need for careful consideration of which probes and conditions can be used when assessing mitochondrial physiology in Drosophila.”

Oliveira MF, Aveiro Y, Couto-Lima CA (2023) A navigation chart to avoid turbulence in Drosophila mitochondrial research. Bioenerg Commun 2023.4. https://doi.org/10.26124/bec:2023-0004

from the O2k-Network »BR Rio de Janeiro Oliveira MF«

Nov- 23 Th

"Thoughtful design and execution of O2k experiments result in data that become a powerhouse for addressing many mitochondrial respiration research questions. "

Walsh MA, Musci RV, Jacobs RA, Hamilton KL (2023) A practical perspective on how to develop, implement, execute, and reproduce high-resolution respirometry experiments: The physiologist's guide to an Oroboros O2k »Bioblast link«

from the O2k-Network »US CO Colorado Springs Jacobs RA« and »US CO Fort Collins Hamilton K«

Nov- 24 Fr

2023 Week 48

Nov- 27 Mo

Nov- 28 Tu

Protocols that SUIT your research question

Using Substrate-Uncoupler-Inhibitor-Titration protocols you can study mitochondrial respiratory control through a sequence of coupling and pathway control states induced by multiple titrations within a single experimental assay.
- »SUIT protocols« and »SUITbrowser«
- communicated by »Schmitt Sabine« and »Timon-Gomez Alba«

Nov- 29 We

Nov- 30 Th

Dec- 01 Fr World AIDS day

"We therefore hypothesized that the susceptibility to HIV-1 infection of FOXO1-inhibited resting T cells could be due to an increased cell metabolism. (...) Results obtained by high-resolution respirometry experiments of [human peripheral blood T cells] PBT (...) showed that [ROUTINE] respiration at the steady state was increased by [FOXO1 inhibitor] AS1842856."

Roux A, Leroy H, De Muylder B, Bracq L, Oussous S, Dusanter-Fourt I, Chougui G, Tacine R, Randriamampita C, Desjardins D, Le Grand R, Bouillaud F, Benichou S, Margottin-Goguet F, Cheynier R, Bismuth G, Mangeney M (2019) FOXO1 transcription factor plays a key role in T cell-HIV-1 interaction. »Bioblast link«

from the O2k-Network »FR Paris Bouillaud F«

»AMI 2023 Jhansi IN« Dec 1-3

Oct 2023

2023 Week 40

Oct- 02 Mo

Oroboros O2k-Workshop IOC162 Schroecken AT Oct 2-7

Oct- 03 Tu

Oroboros O2k-Workshop IOC162 Schroecken AT Oct 2-7

Oct- 04 We

Oroboros O2k-Workshop IOC162 Schroecken AT Oct 2-7

Oct- 05 Th - Breast Cancer Awareness Month

"GK-1 [immunomodulatory peptide] inhibits cellular respiration and induces mitochondrial uncoupling in breast tumors."

Cruz-Gregorio A, Aranda-Rivera AK, Aparicio-Trejo OE, Medina-Campos ON, Sciutto E, Fragoso G, Pedraza-Chaverri J (2022) GK-1 Induces oxidative stress, mitochondrial dysfunction, decreased membrane potential, and impaired autophagy flux in a mouse model of breast cancer. »Bioblast link«

from the O2k-Network »MX Mexico City Pedraza Chaverri J«

Oroboros O2k-Workshop IOC162 Schroecken AT Oct 2-7

Oct- 06 Fr

Oroboros O2k-Workshop IOC162 Schroecken AT Oct 2-7

Oct- 07 Sa

Oroboros O2k-Workshop IOC162 Schroecken AT Oct 2-7

2023 Week 41

Oct- 09 Mo

Oct- 10 Tu

What is the best fluorophore and concentration thereof for measuring mt-membrane potential?

It depends on sample characteristics - test the concentration of different fluorophores (safranin, TMRM and rhodamine 123) with your specific model to evaluate for inhibitory or uncoupling effects on respiration.
- »mt-membrane potential measurement«
- communicated by »Grings Mateus« and »Cardoso Luiza«

Oct- 11 We

The abbreviation 'Dith' has been used previously for Dithionite and is stepwise replaced by the shorter 'Dit'.

- »Dithionite, Dit«

Oct- 12 Th - World Arthritis Day

“Mitochondrial dysfunction did not seem to be a major contributing factor for insulin resistance in RA [rheumatoid arthritis]”

Moellering DR, Smith-Johnston K, Kelley C, Sammy MJ, Benedict J, Brock G, Johnson J, Baskin KK, Jarjour WN, Belury MA, Reiser PJ, Nagareddy PR, Hanaoka BY (2023) Association between skeletal muscle mitochondrial dysfunction and insulin resistance in patients with rheumatoid arthritis: a case-control study. »Bioblast link«

from the O2k-Network »US AL Birmingham Moellering_DR«

Oct- 13 Fr

2023 Week 42

Oct- 16 Mo

»2nd Workshop on Mitochondrial Functional Diagnostics Innsbruck AT«

Oct- 17 Tu

Noticing higher values of J°1 when using the O2k-Chamber sV (0.5 mL) compared to the 2 mL O2k-Chamber?

The instrumental oxygen background flux, J°O₂, is volume-dependent in the closed chamber; therefore, the values of J°1 are about four times higher in the sV chamber.
- »O2k-sV-Module«, »POS calibration-SOP«
- communicated by »Timon-Gomez Alba« and »Leo Elettra«

Oct- 18 We

»EU-METAHEART Kick-off meeting Brussels BE«

Oct- 19 Th

»Life Sciences Partnering 2023 Innsbruck AT«

"MDH2 is a metabolic switch rewiring [electron transfer system] and TCA fuelling"

Molinié T, Cougouilles E, David C, Cahoreau E, Portais JC, Mourier A (2022) MDH2 produced OAA is a metabolic switch rewiring the fuelling of respiratory chain and TCA cycle. »Bioblast link«

from the O2k-Network »FR Bordeaux Mourier A«

Oct- 20 Fr

2023 Week 43

Oct- 23 Mo

“Thanks for this excellent guidance and support thus far.”

Maarman Gerald J, from the O2k-Network Lab »ZA Cape Town Maarman GJ«
- »O2k-Feedback«

Oct- 24 Tu

A theoretical communication from Garcia Menendez on electromagnetic coupling hypothesis.

Garcia Menendez R (2023) Update and a new statement of the electromagnetic coupling hypothesis to explain the translocation of protons in mitochondria, bacteria and chloroplasts. MitoFit Preprints 2023.8. https://doi.org/10.26124/mitofit:2023-0008

Oct- 25 We

Oct- 26 Th

"In collegiate swimmers, (…) a maximal exercise bout (i.e., the bucket test) was associated with minimal changes in cellular-level IO₂ [oxygen flow in isolated PBMCs]. In contrast, due to the robust mobilization of immune cells in response to the maximal exercise bout, the tissue-level IO_2-Tissue [blood] was significantly elevated."

Stampley JE, Cho E, Wang H, Theall B, Johannsen NM, Spielmann G, Irving BA (2023) Impact of maximal exercise on immune cell mobilization and bioenergetics. »Bioblast link«

from the O2k-Network »US LA Baton Rouge Irving BA«

Oct- 27 Fr

2023 Week 44

Oct- 30 Mo

Oct- 31 Tu

How to interpret cytochrome c efficiency

Stimulation of OXPHOS or ET capacity upon addition of cytochrome c provides evidence of cytochrome c loss, indicating damage of the mitochondrial outer membrane, which could be caused by sample preparation or pathological conditions.
- »Cytochrome c control efficiency«
- communicated by »Schmitt Sabine« and »Leo Elettra«

Sep 2023

2023 Week 36

Sep- 04 Mo

Sep- 05 Tu

Higher concentrations of oligomycin can decrease the ET capacity due to an inhibitory effect

Use a lower oligomycin concentration (5-10 nM) to measure LEAK respiration without impairing ET capacity and test to find the optimum concentration for your specific model.
- »Oligomycin in high-resolution respirometry«, »Doerrier 2018 Methods Mol Biol«
- communicated by »Timon-Gomez Alba« and »Grings Mateus«

Sep- 06 We

Sep- 07 Th

"...enhancing mitochondrial function by either overexpressing parkin or bypassing defective mitochondrial complexes has significant beneficial effects in a fly model of HD [Huntington's disease], rescuing neurodegeneration and enhancing viability and lifespan."

Campesan S, Del Popolo I, Marcou K, Straatman-Iwanowska A, Repici M, Boytcheva KV, Cotton VE, Allcock N, Rosato E, Kyriacou CP, Giorgini F (2023) Bypassing mitochondrial defects rescues Huntington's phenotypes in Drosophila. »Bioblast link«

from the O2k-Network »UK Leicester Giorgini F«

Sep- 08 Fr

2023 Week 37

Sep- 11 Mo

Barometric pressure displaying as 0 kPa in the O2 calibration window?

In many cases, this can be solved by by switching off and unplugging the O2k for several minutes.
- »O2k-Barometric Pressure Transducer«
- communicated by »Schmitt Sabine« and »Grings Mateus«

Sep- 12 Tu

Sep- 13 We

Sep- 14 Th

"The higher ROS release rates observed in both the muscle and liver of older individuals may suggest that increased ROS release is evidence of mitochondrial dysfunction during ageing in finches."

Salmón P, Millet C, Selman C, Monaghan P, Dawson NJ (2023) Tissue-specific reductions in mitochondrial efficiency and increased ROS release rates during ageing in zebra finches, Taeniopygia guttata. »Bioblast link«

from the O2k-Network »DE Wilhelmshaven Salmon P«, »UK Glasgow Metcalfe NB«

Sep- 15 Fr - World Lymphoma Awareness Day

"Respirometry assays confirmed that OXPHOS^high Richter transformation cells had a 3.5-fold higher oxygen consumption at ROUTINE respiration and fivefold higher electron transfer system capacity compared to chronic lymphocytic leukemia"

Nadeu F, Royo R, Massoni-Badosa R, Playa-Albinyana H, Garcia-Torre B, et al (2022) Detection of early seeding of Richter transformation in chronic lymphocytic leukemia. »Bioblast link«

from the O2k-Network »ES Barcelona Garcia-Roves PM« and »ES Barcelona IDIBAPS Hospital Clinic«

2023 Week 38

Sep- 18 Mo

World Mitochondrial Disease Week – Sep 18-24

World Mitochondrial Disease Week raises awareness of mitochondrial disease through educational, fundraising and advocacy activities.
»See what is happening near you«

Our Chinese distributor Huawei came to visit us in Innsbruck today.
»CN Beijing Huawei«

Sep- 19 Tu

In 1962, the first report of a patient with a mitochondrial disease was published.

Luft R, Ikkos D, Palmieri G, Ernster L, Afzelius B (1962). A case of severe hypermetabolism of nonthyroid origin with a defect in the maintenance of mitochondrial respiratory control: a correlated clinical, biochemical, and morphological study. »Bioblast link«

Sep- 20 We

Sep- 21 Th

The first mitochondrial DNA mutations associated with disease were described in 1988.

These discoveries were a game changer in the field of mitochondrial diseases.

Holt IJ, Harding A E, Morgan-Hughes JA (1988) Deletions of muscle mitochondrial DNA in patients with mitochondrial myopathies. »Bioblast link«
Wallace DC, Singh G, Lott MT, Hodge JA, Schurr TG, Lezza AM, Elsas LJ 2nd, Nikoskelainen EK (1988) Mitochondrial DNA mutation associated with Leber's hereditary optic neuropathy. »Bioblast link«
Zeviani M, Moraes CT, DiMauro S, Nakase H, Bonilla E, Schon EA, Rowland LP (1988) Deletions of mitochondrial DNA in Kearns-Sayre syndrome. »Bioblast link«

Sep- 22 Fr

2023 Week 39

Sep- 25 Mo

Sep- 26 Tu

Do you know the difference between uncoupled, noncoupled and dyscoupled respiration?

Noncoupled respiration is induced experimentally for evaluation of ET capacity, while uncoupling and dyscoupling are respectively caused by physiological and pathological conditions that exert an influence on proton leak and slip.
- »Noncoupled respiration«, »BEC 2020.01«
- communicated by »Leo Elettra« and »Cardoso Luiza«

Sep- 27 We

Sep- 28 Th

"... systemic transplantation of young MDSPCs [muscle-derived stem/progenitor cells] into progeroid ZMPSTE24-deficient mice preserves skeletal muscle mitochondrial function and fatigue-resistant fibers in a sex-specific manner"

Thompson SD, Barrett KL, Rugel CL, Redmond R, Rudofski A, Kurian J, Curtin JL, Dayanidhi S, Lavasani M (2023) Sex-specific preservation of neuromuscular function and metabolism following systemic transplantation of multipotent adult stem cells in a murine model of progeria. »Bioblast link«

from the O2k-Network »US IL Chicago Dayanidhi S«

Sep- 29 Fr

Aug 2023

2023 Week 31

Jul- 31 Mo

“I really appreciate the amazing customer support of your company.”

Jean-Philippe Leduc-Gaudet, PhD from Université du Québec à Trois-Rivières, CA
- »O2k-Feedback«

Aug- 01 Tu

Respiration might be inhibited in the presence of high concentrations of some saccharides due to the Crabtree effect

It is observed in multiple cell types, such as tumor cells, bacteria or yeast, and it can be measured using SUIT-003 protocols.
- »Crabtree effect«, »SUIT-003«
- communicated by »Timon-Gomez Alba« and »Leo Elettra«

Aug- 02 We

Aug- 03 Th

"Hepatocyte-derived cells maintain cellular and mitochondrial Ca²⁺ concentrations within strictly controlled ranges, maximizing electron transport capacity by promoting the Goldilocks Ca²⁺ “sweet spot” for oxidative phosphorylation activity: neither too much nor too little, but just right."

Vilas-Boas EA, Cabral-Costa JV, Ramos VM, Caldeira da Silva CC, Kowaltowski AJ (2023) Goldilocks calcium concentrations and the regulation of oxidative phosphorylation: too much, too little, or just right. »Bioblast link«

from the O2k-Network »BR Sao Paulo Kowaltowski AJ«

Aug- 04 Fr

2023 Week 32

Aug- 07 Mo

Aug- 08 Tu

An overview of the “do’s and don’ts” when it comes to assessing mitochondrial function in Drosophila

Rodríguez E, Bettinazzi S, Inwongwan S, Camus MF, Lane N (2023) Harmonizing protocols to measure Drosophila respiratory function in mitochondrial preparations. Bioenerg Commun 2023.3. https://doi.org/10.26124/bec:2023-0003

from the O2k-Network »UK London Lane N«

Aug- 09 We

Aug- 10 Th

"Reducing lipid hydroxides by GPX4 overexpression ameliorates the majority of muscle atrophy and weakness phenotypes present in mouse models of sarcopenia"

Czyżowska A, Brown J, Xu H, Sataranatarajan K, Kinter M, Tyrell VJ, O'Donnell VB, Van Remmen H (2023) Elevated phospholipid hydroperoxide glutathione peroxidase (GPX4) expression modulates oxylipin formation and inhibits age-related skeletal muscle atrophy and weakness. »Bioblast link«

from the O2k-Network »US OK Oklahoma City Van Remmen H«

Aug- 11 Fr

2023 Week 33

Aug- 14 Mo

What do you do if values, symbols, and/or units are not correctly displayed in the DatLab software?

It is possible to retrieve the values, symbols and/or correct units by updating the Windows control panel settings.
- » Troubleshooting for problems with text in DatLab«
- communicated by »Cecatto Cristiane« and »Timon-Gomez Alba«

Aug- 15 Tu

Aug- 16 We

Aug- 17 Th

"... species-specific differences in cardiac metabolic capacity [...] may influence the acclimation potential of notothenioid fishes to changing environmental conditions."

Mandic M, Frazier AJ, Naslund AW, Todgham AE (2022) A comparative and ontogenetic examination of mitochondrial function in antarctic notothenioid species. »Bioblast link«

Aug- 18 Fr

2023 Week 34

Aug- 21 Mo

Aug- 22 Tu

Are pyruvate, glutamate, and malate substrates of Complex I?

No, NADH is a Complex I substrate. However, pyruvate, glutamate and malate are used in high-resolution respirometry as substrates for mitochondrial dehydrogenases to generate NADH in the mitochondrial matrix because NADH cannot cross the mitochondrial inner membrane.
- »NADH electron transfer-pathway state« and »Complex I«
- communicated by »Cardoso Luiza« and »Grings Mateus«

Aug- 23 We

Aug- 24 Th

"Understanding acylcarnitine metabolic and signaling pathways provides a better rationale for selecting targets for therapeutic interventions."

Dambrova M, Makrecka-Kuka M, Kuka J, Vilskersts R, Nordberg D, Attwood MM, Smesny S, Sen ZD, Guo AC, Oler E, Tian S, Zheng J, Wishart DS, Liepinsh E, Schioth HB. (2022) Acylcarnitines: Nomenclature, Biomarkers, Therapeutic Potential, Drug Targets, and Clinical Trials. »Bioblast link«

from the O2k-Network »LV Riga Liepins E«

Aug- 25 Fr

2023 Week 35

Aug- 28 Mo

Can I compare data sets recorded with different sensor gain settings?

Oxygen signals obtained after proper calibration are independent of the gain settings, therefore oxygen concentrations (and fluxes) can be compared, as long as the corresponding air and zero calibrations are applied.
- »Gain of the O2 channel«
- communicated by »Baglivo Eleonora« and »Leo Elettra«

Aug- 29 Tu

"Altogether, our results show that chronic hypoxia optimizes the efficiency of mitochondrial ATP synthesis in the retrosplenial cortex of mice. Contrastingly, rats sustain the production of ATP only by increasing mitochondrial content and altering mitochondrial dynamics, suggesting drastic mitochondrial malfunctions."

Arias-Reyes C, Aliaga-Raduán F, Pinto-Aparicio R, Joseph V, Soliz J (2023) Mitochondrial plasticity in the retrosplenial cortex enhances ATP synthesis during acclimatization to hypoxia in mice but not in rats. MitoFit Preprints 2023.6. https://doi.org/10.26124/mitofit:2023-0006

from the O2k-Network »CA Quebec Soliz J«

Aug- 30 We

Aug- 31 Th

"Our study showed that salinity modulates the mitochondrial responses to long-term and acute hypoxia exposure in an euryhaline intertidal bivalve C. gigas."

Steffen JBM, Sokolov EP, Sokolova IM (2023) Combined effects of salinity and intermittent hypoxia on mitochondrial capacity and reactive oxygen species efflux in the Pacific oyster, Crassostrea gigas »Bioblast link«

from the O2k-Network »DE Rostock Sokolova I«

Sep- 01 Fr

Jul 2023

2023 Week 27

Jul- 03 Mo

Jul- 04 Tu

The function of mitochondrial respiratory supercomplexes is still under discussion

Although their structure has been described in different species, their function is still unclear. Come to MiPschool 2023 to find out about the latest discoveries.
- »Respiratory complexes and supercomplexes« and »MiPschool 2023«
- communicated by »Timon-Gomez Alba« and »Cecatto Cristiane«

Jul- 05 We

Jul- 06 Th

"G3 MB [Group 3 medulloblastoma] cells demonstrate exquisite sensitivity towards inhibitors of complex-I in the electron transport [system (ETS)] but not to other metabolism-targeting agents, including glycolytic inhibitors."

Martell E, Kuzmychova H, Kaul E, Senthil H, Chowdhury SR, Morrison LC, Fresnoza A, Zagozewski J, Venugopal C, Anderson CM, Singh SK, Banerji V, Werbowetski-Ogilvie TE, Sharif T (2023) Metabolism-based targeting of MYC via MPC-SOD2 axis-mediated oxidation promotes cellular differentiation in group 3 medulloblastoma. »Bioblast link«

from the O2k-Network »CA Winnipeg Banerji V«

Jul- 07 Fr

2023 Week 28

Jul- 10 Mo

»PBMC-Workshop Innsbruck AT«

Jul- 11 Tu

Here is a system of terms and abbreviations for mitochondrial respiratory pathway and coupling control states.

Although states and rates are distinguished by different symbols for coupling control states, such a distinction is not suggested for pathway control states and rates to avoid handing and overwhelming number of different symbols.
- »MitoPedia: Pathway and coupling control states«

Jul- 12 We

Is the ambiguity crisis a contemporary crisis comparable to the credibility or reproducibility crisis?

This is discussed in Gnaiger E (2023) Complex II ambiguities ― FADH₂ in the electron transfer system. https://doi.org/10.26124/mitofit:2023-0003.v6
- »MitoPedia: Ambiguity crisis«

Jul- 13 Th

Triplefin fish species have different ROS production and electron leakage according to their tolerance to hypoxia.

Devaux JBL, Hedges CP, Birch N, Herbert N, Renshaw GMC, Hickey AJR (2023) Electron transfer and ROS production in brain mitochondria of intertidal and subtidal triplefin fish (Tripterygiidae). »Bioblast link«

Jul- 14 Fr

Jul- 15 Sa

»2nd Mitochondria Conference 2023 Lisbon PT« Jul 15-18

2023 Week 29

Jul- 17 Mo

Are you planning to perform several measurements in one day using the same O2k-chamber?

To avoid any possible contamination by inhibitors in the subsequent measurements, the O2k-chamber needs to be cleaned each time with the standard protocol AfterUse or AfterUse_inhibitors.
- »O2k-cleaning - Sections 2.3 and 2.4«
- communicated by »Timon-Gomez Alba« and »Cecatto Cristiane«

Jul- 18 Tu

Jul- 19 We

Jul- 20 Th

"A cancer-selective Na⁺ increase correlated with HCC [hepatocellular carcinoma] cell killing, and a slower growth rate of HCC xenograft tumors."

Clemente N, Baroni S, Fiorilla S, Tasso F, Reano S, Borsotti C, Ruggiero MR, Alchera E, Corrazzari M, Walker G, Follenzi A, Crich SG, Carini R (2023) Boosting intracellular sodium selectively kills hepatocarcinoma cells and induces hepatocellular carcinoma tumor shrinkage in mice. »Bioblast link«

from the O2k-Network »IT Novara Filigheddu N«

Jul- 21 Fr

Jul- 22 Sa

Jul- 23 Su

MiPschool Obergurgl 2023 Jul 23-27

2023 Week 30

Jul- 24 Mo

MiPschool Obergurgl 2023 Jul 23-27

Jul- 25 Tu

MiPschool Obergurgl 2023 Jul 23-27

Body mass excess (BME) or body mass index (BMI)?

BME provides an evidence-based index of underweight, overweight and obesity across all heights and ages, in contrast to BMI. Try our BME calculator.
- »Body mass excess calculator« and »Body mass excess«
- communicated by »Timon-Gomez Alba« and »Cecatto Cristiane«

Jul- 26 We

MiPschool Obergurgl 2023 Jul 23-27

Jul- 27 Th

MiPschool Obergurgl 2023 Jul 23-27

15^th MiPconference: Bioenergetics Communications on mitObesity and Healthy Aging Obergurgl 2023 Jul 27-30

Mitochondrial function abnormalities in patients with heart failure with preserved ejection fraction are associated with exercise intolerance and represent novel therapeutic targets.

Scandalis L, Kitzman DW, Nicklas BJ, Lyles M, Brubaker P, Nelson MB, Gordon M, Stone J, Bergstrom J, Neufer PD, Gnaiger E, Molina AJA (2023) Skeletal muscle mitochondrial respiration and exercise intolerance in patients with heart failure with preserved ejection fraction. »Bioblast link«

Jul- 28 Fr

15^th MiPconference: Bioenergetics Communications on mitObesity and Healthy Aging Obergurgl 2023 Jul 27-30

Jul- 29 Sa

15^th MiPconference: Bioenergetics Communications on mitObesity and Healthy Aging Obergurgl 2023 Jul 27-30

Jul- 30 Su

15^th MiPconference: Bioenergetics Communications on mitObesity and Healthy Aging Obergurgl 2023 Jul 27-30

Jun 2023

2023 Week 23

Jun- 05 Mo

Jun- 06 Tu

»Health Innovation Summit 2023 Vienna AT«

13:30-14:30 Erich Gnaiger discussion panel member at Health Innovation Summit 2023 Vienna AT - The BMI gender data gap: Cellular energy metabolism is fired by mitochondria. Their respiratory capacity decreases with obesity. But the WHO defines obesity with an adult-white-male medicine bias inherent in the body mass index.

Jun- 07 We

»ESCI 2023 Prague CZ« Jun 7-9

Jun- 08 Th

»International GIBB Meeting 2023 Riva del Garda IT« Jun 8-10

13:00 Baglivo Eleonora poster session at ESCI 2023 Prague CZ - The cytochrome c test for respirometric quality control of the integrity of the mitochondrial outer membrane - Session S01 - Mitochondrial Biology

13:00 Leo Elettra poster session at ESCI 2023 Prague CZ - High-resolution respirometry is comparable in 0.5 mL and 2.0 mL chamber volumes: studies with platelets, permeabilized fibroblasts, and isolated mitochondria - Session S01 - Mitochondrial Biology

16:00-16:15 Timon-Gomez Alba oral presentation at International GIBB Meeting 2023 Riva del Garda IT - A reference substrate-uncoupler-inhibitor titration protocol to create a mitochondrial pathway and coupling control database - Session 1 - Redox reactions and dynamics of energy-transducing membranes

Jun- 09 Fr

14:30-15:00 Gnaiger Erich Plenary lecture 3 at International GIBB Meeting 2023 Riva del Garda IT - Nonlinearity of the proton leak and membrane potential - protonmotive pressure as a unifying concept

Jun- 10 Sa

Jun- 11 Su

»EUROMIT2023 Bologna IT« Jun 11-15

2023 Week 24

Jun- 12 Mo

»EUROMIT2023 Bologna IT« Jun 11-15

15:30 Timon-Gomez Alba poster session at EUROMIT2023 Bologna IT - Mitochondrial injury in warm ischemia studied by high-resolution respirometry - Session topic: Modelling pathogenic mechanisms: OXPHOS, metabolic rewiring and tissue specificity. Poster #11

18:00 Gnaiger Erich poster session at EUROMIT2023 Bologna IT - Off-target effects of etomoxir: inhibition of mitochondrial Complex I and fatty acid oxidation - Session topic: Modelling pathogenic mechanisms: OXPHOS, metabolic rewiring and tissue specificity. Poster #135

Jun- 13 Tu

12:45-13:45 Gnaiger Erich lunch workshop at EUROMIT2023 Bologna IT - Mitochondrial bioenergetics - a quantitative analytical and diagnostic approach - Sala Europa

Jun- 14 We

Jun- 15 Th

"Obesity has an opposing association with mitochondrial respiration in adipose- and liver tissue with no overall association with NAFLD (nonalcoholic fatty liver disease) severity."

Pedersen JS, Rygg MO, Chrøis K, Sustarsic EG, Gerhart-Hines Z, Wever Albrechtsen NJ, Serizawa RR, Kristiansen VB, Basse AL, Boilesen AEB, Olsen BH, Hansen T, Gluud LL, Madsbad S, Larsen S, Bendtsen F, Dela F (2022) Influence of NAFLD and bariatric surgery on hepatic and adipose tissue mitochondrial biogenesis and respiration. »Bioblast link«

from the O2k-Network »DK Copenhagen Gerhart-Hines Z« and »DK Copenhagen Larsen S«

Jun- 16 Fr

2023 Week 25

Jun- 19 Mo