Categories of SUIT protocols

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MitoPedia

Categories of SUIT protocols

Description

SUIT-catg MitoPathway types.jpg

Categories of SUIT protocols group SUIT protocols according to all substrate types involved in a protocol (F, N, S, Gp), independent of the sequence of titrations of substrates and inhibitors which define the Electron transfer-pathway states. The N-type substrates are listed in parentheses, independent of the sequence of titrations. ROX states may or may not be included in a SUIT protocol, which does not change its category. Similarly, the CIV assay may or may not be added at the end of a SUIT protocol, without effect on the category of a SUIT protocol.

  • F - ET-pathway-level 5: FADH2-linked substrates (FAO) with obligatory support by the N-linked pathway.
  • N - ET-pathway-level 4: NADH-linked substrates (CI-linked).
  • S - ET-pathway-level 3: Succinate (CII-linked).
  • Gp - ET-pathway-level 3: Glycerophosphate (CGpDH-linked).
  • Y(X)- In the SUIT general protocols Y makes reference to the ET-pathway state and X to the combination os substrates added for the corresponding pathway.

» MiPNet article

Abbreviation: SUIT-catg

Reference: MiPNet21.06 SUIT RP


MitoPedia concepts: SUIT concept 

Categories of SUIT protocols and ET-pathway control states

Publications in the MiPMap
Gnaiger E (2017) Categories of SUIT protocols and ETS pathway control states. MiPNet 2017-03-12; original 2016-03-20; edited 2016-08-21.

» MiPNet21.06 SUIT RP

Oroboros (2017) MiPNet

Abstract: The complexity of SUIT protocols is primarily determined by the large number of Electron transfer-pathway states and fuel substrates involved, compared to only three well defined coupling control states. Whereas the SUIT protocol names specify all sequential steps in a SUIT protocol, the categories of SUIT protocols reduce this diversity to a list of substrate types involved.


O2k-Network Lab: AT Innsbruck Oroboros


Labels: MiParea: Instruments;methods 





Pathway: F, N, S, Gp, CIV, NS, Other combinations, ROX  HRR: Theory 


SUIT protocols

Towards a library of SUIT protocols

At the present stage of development of the Library of SUIT protocols as part of the MitoFit Quality Control System, the substrate types linked to five ET-pathway types are considered.
  • F-junction on the pathway level of converging FADH2- and NADH-linked dehydrogenases, including beta-oxidationthe and segments of the TCA cycle.
  • N-junction on the pathway level of NADH-linked dehydrogenases, including the TCA cycle.
  • Q-junction on the pathway level of electron transfer complexes converging at the Q-junction (S and Gp).
  • Tm on the single step level of cytochrome c oxidase (CIV), the terminal step in the aerobic electron transfer-pathway. Tm can be included or excluded at the end of a SUIT protocol. To simplify the categorization, Tm is not considered in this system of SUIT protocols.


Single pathway type

ET-pathway-level 1: CIV

SUIT-catg CIV.jpg

ET-pathway-level 3: S, CII-pathway to Q

SUIT-catg S.jpg

ET-pathway-level 3: Gp, CGpDH-pathway to Q

SUIT-catg Gp.jpg

ET-pathway-level 4: N, N-junction or CI-pathway to Q

SUIT-catg N.jpg

ET-pathway-level 5: F, FAO, F-junction, CETF- supported by CI-pathway to Q

SUIT-catg F.jpg


Multiple ET-pathway with FNSGp

SUIT-catg FNSGpCIV.jpg


Multiple ET pathways with NS

NS

SUIT-catg NS.jpg

FNS

SUIT-catg FNS.jpg

NSGp

SUIT-catg NSGp.jpg

FNSGp

SUIT-catg FNSGp.jpg


Multiple ET pathways with N (without S)

FN

SUIT-catg FN.jpg

NGp

SUIT-catg NGp.jpg

FNGp

SUIT-catg FNGp.jpg


Multiple ET pathways without N

Addition of malate alone (M without P or G) is not considered as substrate type N. However, high mt-malic enzyme activity requires a change of this concept, when M alone represents an ET-pathway ccompetent substrate state. Low concentration of M may be used to support FAO, whereas a higher concentration of M may be required for N-junction respiratory capacity to override FAO capacity; this needs corresponding kinetic analyses (SUIT test protocols).

FS

SUIT-catg FS.jpg

FGp

SUIT-catg FGp.jpg

SGp

SUIT-catg SGp.jpg

FSGp

SUIT-catg FSGp.jpg