Difference between revisions of "Uncoupling-control ratio"
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{{MitoPedia | {{MitoPedia | ||
|abbr=UCR | |abbr=UCR | ||
|description=The '''uncoupling control ratio''' | |description=The '''uncoupling-control ratio''' UCR is the ratio of ET-pathway/ROUTINE-respiration (''E/R'') in living cells, evaluated by careful [[uncoupler]] titrations ([[Steinlechner-Maran 1996 Am J Physiol Cell Physiol|Steinlechner et al 1996]]).ย Compare [[ROUTINE-control ratio]] (''R/E'') [[Gnaiger 2008 POS|(Gnaiger 2008)]]. | ||
|info=[[Steinlechner-Maran 1996 | |info=[[Steinlechner-Maran 1996 Am J Physiol Cell Physiol]] | ||
}} | }} | ||
{{ | == Biochemical coupling efficiency: from 0 to <1 == | ||
::::* ''More details:'' [[Biochemical coupling efficiency]] | |||
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| | == Keywords == | ||
{{Template:Keywords: Coupling control}} | |||
{{Template:Keywords: Uncoupling}} | |||
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{{MitoPedia concepts | |||
|mitopedia concept=Respiratory control ratio | |||
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{{MitoPedia methods | |||
|mitopedia method=Respirometry | |||
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{{MitoPedia O2k and high-resolution respirometry|type=Respiration | |||
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[[Category:Coupling control]] |
Latest revision as of 11:40, 11 November 2020
Description
The uncoupling-control ratio UCR is the ratio of ET-pathway/ROUTINE-respiration (E/R) in living cells, evaluated by careful uncoupler titrations (Steinlechner et al 1996). Compare ROUTINE-control ratio (R/E) (Gnaiger 2008).
Abbreviation: UCR
Reference: Steinlechner-Maran 1996 Am J Physiol Cell Physiol
Biochemical coupling efficiency: from 0 to <1
- More details: Biochemical coupling efficiency
Keywords
4-compartmental OXPHOS model. (1) ET capacity E of the noncoupled electron transfer system ETS. OXPHOS capacity P is partitioned into (2) the dissipative LEAK component L, and (3) ADP-stimulated P-L net OXPHOS capacity. (4) If P-L is kinetically limited by a low capacity of the phosphorylation system to utilize the protonmotive force pmF, then the apparent E-P excess capacity is available to drive coupled processes other than phosphorylation Pยป (ADP to ATP) without competing with Pยป.
- Bioblast links: Coupling control - >>>>>>> - Click on [Expand] or [Collapse] - >>>>>>>
1. Mitochondrial and cellular respiratory rates in coupling-control states
Respiratory rate | Defining relations | Icon | |
---|---|---|---|
OXPHOS capacity | P = Pยด-Rox | mt-preparations | |
ROUTINE respiration | R = Rยด-Rox | living cells | |
ET capacity | E = Eยด-Rox | ยป Level flow | |
ยป Noncoupled respiration - Uncoupler | |||
LEAK respiration | L = Lยด-Rox | ยป Static head | |
ยป LEAK state with ATP | |||
ยป LEAK state with oligomycin | |||
ยป LEAK state without adenylates | |||
Residual oxygen consumption Rox | L = Lยด-Rox |
2. Flux control ratios related to coupling in mt-preparations and living cells
FCR | Definition | Icon | |
---|---|---|---|
L/P coupling-control ratio | L/P | ยป Respiratory acceptor control ratio, RCR = P/L | |
L/R coupling-control ratio | L/R | ||
L/E coupling-control ratio | L/E | ยป Uncoupling-control ratio, UCR = E/L (ambiguous) | |
P/E control ratio | P/E | ||
R/E control ratio | R/E | ยป Uncoupling-control ratio, UCR = E/L | |
net P/E control ratio | (P-L)/E | ||
net R/E control ratio | (R-L)/E |
3. Net, excess, and reserve capacities of respiration
Respiratory net rate | Definition | Icon |
---|---|---|
P-L net OXPHOS capacity | P-L | |
R-L net ROUTINE capacity | R-L | |
E-L net ET capacity | E-L | |
E-P excess capacity | E-P | |
E-R reserve capacity | E-R |
4. Flux control efficiencies related to coupling-control ratios
- ยป Flux control efficiency jZ-Y
- ยป Background state
- ยป Reference state
- ยป Metabolic control variable
Coupling-control efficiency | Definition | Icon | Canonical term | ||
---|---|---|---|---|---|
P-L control efficiency | jP-L | = (P-L)/P | = 1-L/P | P-L OXPHOS-flux control efficiency | |
R-L control efficiency | jR-L | = (R-L)/R | = 1-L/R | R-L ROUTINE-flux control efficiency | |
E-L coupling efficiency | jE-L | = (E-L)/E | = 1-L/E | E-L ET-coupling efficiency ยป Biochemical coupling efficiency | |
E-P control efficiency | jE-P | = (E-P)/E | = 1-P/E | E-P ET-excess flux control efficiency | |
E-R control efficiency | jE-R | = (E-R)/E | = 1-R/E | E-R ET-reserve flux control efficiency |
5. General
- ยป Basal respiration
- ยป Cell ergometry
- ยป Dyscoupled respiration
- ยป Dyscoupling
- ยป Electron leak
- ยป Electron-transfer-pathway state
- ยป Hyphenation
- ยป Oxidative phosphorylation
- ยป Oxygen flow
- ยป Oxygen flux
- ยป Permeabilized cells
- ยป Phosphorylation system
- ยป Proton leak
- ยป Proton slip
- ยป Respiratory state
- ยป Uncoupling
- Bioblast links: Uncoupling - >>>>>>> - Click on [Expand] or [Collapse] - >>>>>>>
- Specific
- ยป Artefacts by single dose uncoupling
- ยป ATP synthase
- ยป CCCP
- ยป Coupling-control protocol
- ยป DNP
- ยป Dyscoupled respiration
- ยป FCCP
- ยป Is respiration uncoupled - noncoupled - dyscoupled?
- ยป Noncoupled respiration: Discussion
- ยป Uncoupler
- ยป Uncoupled respiration - see ยป Noncoupled respiration
- ยป Uncoupling proteins
- ยป Uncoupling protein 1
- ยป Uncoupler titrations - Optimum uncoupler concentration
- Specific
- Respiratory states and control ratios
- ยป Biochemical coupling efficiency
- ยป Coupling-control state
- ยป Electron-transfer-pathway state
- ยป Electron-transfer pathway
- ET capacity
- ยป E-L coupling efficiency
- ยป Flux control efficiency
- ยป Flux control ratio
- ยป LEAK-control ratio
- ยป LEAK respiration
- ยป Noncoupled respiration
- ยป OXPHOS
- ยป OXPHOS capacity; ยป State 3
- ยป OXPHOS-control ratio, P/E ratio
- ยป Respiratory acceptor control ratio
- ยป ROUTINE-control ratio
- ยป ROUTINE respiration
- ยป ROUTINE state
- ยป State 3u
- ยป State 4
- ยป Uncoupling-control ratio UCR
- Respiratory states and control ratios
- Gnaiger E et al โ MitoEAGLE Task Group (2020) Mitochondrial physiology. Bioenerg Commun 2020.1. https://doi.org/10.26124/bec:2020-0001.v1
- Gnaiger E (2020) Mitochondrial pathways and respiratory control. An introduction to OXPHOS analysis. 5th ed. Bioenerg Commun 2020.2. https://doi.org/10.26124/bec:2020-0002
- General (alphabetical order)
- Other keyword lists
MitoPedia concepts:
Respiratory control ratio
MitoPedia methods:
Respirometry