SUIT-019: Difference between revisions
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|abbr=Pal+Oct+P+G_OXPHOS | |abbr=Pal+Oct+P+G_OXPHOS+S+Rot_ET | ||
|description=[[File:1PalM;2D;3Oct;4P;5G;6U;7S;8Rot-.png|450px]] | |description=[[File:1PalM;2D;3Oct;4P;5G;6U;7S;8Rot-.png|450px]] | ||
|info='''A: | |info='''A: |
Revision as of 13:41, 5 June 2019
Description
Abbreviation: Pal+Oct+P+G_OXPHOS+S+Rot_ET
Reference: A:
- SUIT protocol pattern: 1PalM;2D;3Oct;4P;5G;6U;7S;8Rot-
SUIT-019 gives information on F-pathway in LEAK and OXPHOS states. Moreover, this SUIT protocol allows the pathway control evaluation of FN in OXPHOS state and FN, FNS and S in ET state.
Communicated by Cardoso LH, Doerrier C, Komlodi T, Gnaiger E (last update 2019-06-05)
Specific SUIT protocols
- SUIT-019 O2 pfi D045 for permeabilized fibers (source: Lemieux et al 2011)
Steps and respiratory states
Step | State | Pathway | Q-junction | Comment - Events (E) and Marks (M) |
---|---|---|---|---|
1PalM | PalML(n) | F(N) | CETF | 1PalM
|
2D | PalMP | F(N) | CETF | 1PalM;2D
|
2c | PalMP | F(N) | CETF | 1PalM;2D;2c
|
3Oct | PalOctMP | F(N) | CETF | 1PalM;2D;2c;3Oct
|
4P | PalOctPMP | FN | CETF&CI | 1PalM;2D;2c;3Oct;4P
|
5G | PalOctPGMP | FN | CETF&CI | 1PalM;2D;2c;3Oct;4P;5G
|
6U | PalOctPGME | FN | CETF&CI&II | 1PalM;2D;2c;3Oct;4P;5G;6U
|
7S | PalOctPGMSE | FNS | CI&CII | 1PalM;2D;2c;3Oct;4P;5G;6U;7S
|
8Rot | SE | S | CII | 1PalM;2D;2c;3Oct;4P;5G;6U;7S;8Rot
|
9Ama | ROX | 1PalM;2D;2c;3Oct;4P;5G;6U;7S;8Rot;9Ama
|
Step | Respiratory state | Pathway control | ET-Complex | Comment |
---|---|---|---|---|
## AsTm | AsTmE | CIV | CIV | |
## Azd | CHB |
- Bioblast links: SUIT protocols - >>>>>>> - Click on [Expand] or [Collapse] - >>>>>>>
- Coupling control
- Pathway control
- Β» Electron transfer pathway
- Β» Fatty acid oxidation pathway control state, F
- Β» NADH electron transfer-pathway state, N
- Β» Succinate pathway control state, S
- Β» NS-pathway control state, NS
- Β» Glycerophosphate pathway control state, Gp
- Β» Complex IV single step, CIV
- Β» Anaplerotic pathway control state
- Pathway control
- Main fuel substrates
- Β» Glutamate, G
- Β» Glycerophosphate, Gp
- Β» Malate, M
- Β» Octanoylcarnitine, Oct
- Β» Pyruvate, P
- Β» Succinate, S
- Main fuel substrates
- Glossary
Strengths and limitations
- SUIT-019 allows the study of OXPHOS capacity in the fatty acid oxidation (F) and fatty acid oxidation&NADH pathway (FN) control states.
- + The protocol provides information on F-pathway (in presence of palmitoylcarnitine, and palmitoylcarnitine&octanoylcarnitine) in LEAK and OXPHOS states. In human heart muscle, the addition of octanoylcarnitine to palmitoylcarnitine&malate increased OXPHOS by 26% (Lemieux H et al 2011).
- + SUIT-019 provides information of the linear coupling (L-P) in FN-pathway.
- + Maximum ET capacity in FNS-pathway is obtained in SUIT-019.
- + P/E for FN-pathway (5G/6U) can be calculated.
- + Reasonable duration of the experiment.
- - Octanoylcarnitine and palmitoylcarnitine can have an uncoupling effect on OXPHOS state if their concentration is not optimized.
- - In the presence of anaplerotic pathways F-pathway is overestimated.
- - CIV activity is not measured, to save experimental time.
Compare SUIT protocols
- SUIT-002 (RP2): SUIT protocol specially designed to give information on F-pathway in OXPHOS state avoiding FAO overestimation in the presence of anaplerotic pathways. Moreover, the pathway control in OXPHOS state (F, F(N), FN, FNS, FNSGp pathways) and in ET state (FNSGp and SGp) can be evaluated by using this SUIT protocol.
References
Year | Reference | Organism | Tissue;cell | |
---|---|---|---|---|
Lemieux 2011 Int J Biochem Cell Biol | 2011 | Lemieux H, Semsroth S, Antretter H, HΓΆfer D, Gnaiger E (2011) Mitochondrial respiratory control and early defects of oxidative phosphorylation in the failing human heart. Int J Biochem Cell Biol 43:1729β38. https://doi.org/10.1016/j.biocel.2011.08.008 | Human | Heart |
MitoPedia concepts:
MiP concept,
SUIT protocol,
Recommended
MitoPedia methods:
Respirometry