Difference between revisions of "SUIT-025"
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::: '''[[Categories of SUIT protocols |SUIT-category]]:''' FNS(Oct,PGM) | ::: '''[[Categories of SUIT protocols |SUIT-category]]:''' FNS(Oct,PGM) | ||
::: '''[[SUIT protocol pattern]]:''' lineal 1D;2M | ::: '''[[SUIT protocol pattern]]:''' lineal 1D;2M;3Oct;3c;4M;5P;6G;7S;8Rot- | ||
The SUIT-025 protocol is based on [[SUIT-002]] specially designed to give information on [[Fatty_acid_oxidation_pathway_control_state|F-pathway]] in [[Oxidative phosphorylation|OXPHOS state]] avoiding FAO overestimation in the presence of [[Anaplerosis|anaplerotic]] pathways. Moreover, the pathway control in OXPHOS state ([[Fatty_acid_oxidation_pathway_control_state|F]], F(N), [[FN]], [[FNS]], pathways) can be evaluated by using this SUIT protocol. | The SUIT-025 protocol is based on [[SUIT-002]] specially designed to give information on [[Fatty_acid_oxidation_pathway_control_state|F-pathway]] in [[Oxidative phosphorylation|OXPHOS state]] avoiding FAO overestimation in the presence of [[Anaplerosis|anaplerotic]] pathways. Moreover, the pathway control in OXPHOS state ([[Fatty_acid_oxidation_pathway_control_state|F]], F(N), [[FN]], [[FNS]], pathways) can be evaluated by using this SUIT protocol. |
Revision as of 12:22, 14 May 2019
Description
Abbreviation: FNS(Oct,PGM)
Reference: »Versions
- SUIT-category: FNS(Oct,PGM)
- SUIT protocol pattern: lineal 1D;2M;3Oct;3c;4M;5P;6G;7S;8Rot-
The SUIT-025 protocol is based on SUIT-002 specially designed to give information on F-pathway in OXPHOS state avoiding FAO overestimation in the presence of anaplerotic pathways. Moreover, the pathway control in OXPHOS state (F, F(N), FN, FNS, pathways) can be evaluated by using this SUIT protocol.
Communicated by Doerrier C (last update 2019-05-14)
== Specific SUIT protocols == SUIT-025_O2_mt_D057
SUIT-025 O2 mt D057
- SUIT-025 O2 mt D057 for isolated mitochondria, tissue homogenate and permeabilized cells (already permeabilized when they are added to the chamber)
Steps and respiratory states
Step | State | Pathway | Q-junction | Comment - Events (E) and Marks (M) |
---|---|---|---|---|
1D | ROX | 1D
| ||
2M.1 | ||||
3Oct | OctMP | F | FAO | 1D;2M.1;3Oct
|
3c | OctMcP | F | FAO | 1D;2M.1;3Oct;3c
|
4M2 | OctMP | F(N) | FAO | 1D;2M.1;3Oct;4M2
|
5P | OctPMP | FN | FAO&CI | 1D;2M.1;3Oct;4M2;5P
|
6G | OctPGMP | FN | FAO&CI | 1D;2M.1;3Oct;4M2;5P;6G
|
7S | OctPGMSP | FNS | FAO&CI&II | 1D;2M.1;3Oct;4M2;5P;6G;7S
|
8Rot | SP | S | CII | 1D;2M.1;3Oct;4M2;5P;6G;7S;8Rot
|
9Ama | ROX | 1D;2M.1;3Oct;4M2;5P;6G;7S;8Rot;9Ama
|
- Bioblast links: SUIT protocols - >>>>>>> - Click on [Expand] or [Collapse] - >>>>>>>
- Coupling control
- Pathway control
- Main fuel substrates
- » Glutamate, G
- » Glycerophosphate, Gp
- » Malate, M
- » Octanoylcarnitine, Oct
- » Pyruvate, P
- » Succinate, S
- Main fuel substrates
- Glossary
Strengths and limitations
- +
- -
Compare SUIT protocols
- SUIT-002 (the reference protocol 2) differs from SUIT-025 in the coupling and pathway control states. SUIT-002 allows the evaluation of pathway control in OXPHOS state (F, F(N), FN, FNS, FNSGp pathways) and in ET state (FNSGp and SGp) whereas in SUIT-025 only F, F(N), FN and FNS pathways in OXPHOS state can be evaluated.
References
MitoPedia concepts: MiP concept, SUIT protocol, Recommended
MitoPedia methods:
Respirometry