Cyanide

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MitoPedia

Cyanide

Description

Cyanide (usually added as KCN) is a competitive inhibitor of cytochcrome c oxidase (CIV). Inhibition is reversed by pyruvate and high oxygen levels.

Abbreviation: KCN

Reference: MiPNet09.12



MitoPedia topics: Inhibitor 

Application in HRR

Inhibitor titrations, CIV-assay, alternative oxidase and ROX


Preparation of 20 mM KCN stock (in 45mM Trizma base)

Kcn(KCN): Fluka 60178, 100 g, store at R.T.; FW = 65.12., Trizma base: Sigma T6066, 100 g, store at R.T.; FW = 121.14
Caution: KCN is photosensitive and hygroscopic; HCN is volatile and very toxic! It is recommended to prepare fresh KCN solution although it can be safely stored at -20°C. To avoid KCN evaporation, it is recommended to use alkaline buffers (such as 45 mM Trizma base, pH >10).
Preparation of 45 mM Trizma base:
  1. Weigh 545 mg of Trizma base
  2. Dissolve it in 100 mL of H20.
Preparation of 20 mM KCN:
  1. Weigh 5.2 mg of KCN and dissolve in 4 mL of 45 mM Trizma base.
  2. Divide into 500 µl aliquots (use dark glass vials).
  3. Store frozen at -20 °C.


O2k manual titrations MiPNet09.12 O2k-Titrations
  • Titration volume: 100 µL of 20 mM stock (2 mL O2k-chamber).
  • Final concentration: 1 mM.