MiPNet09.12 O2k-Titrations

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O2k manual titrations: SUIT protocols with mitochondrial preparations.

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Oroboros (2019-10-23) Mitochondr Physiol Network

Abstract: Oroboros (2019) O2k manual titrations: SUIT protocols with mitochondrial preparations. Mitochondr Physiol Network 09.12(19): 1-4.

A list of substrates, uncouplers, and inhibitors used in SUIT protocols in high-resolution respirometry, with information on abbreviations, stock concentrations, final experimental concentrations, volumes titrated into the 2 mL O2k-chamber and 0.5 mL O2k-Chamber sV (O2k-sV-Module), and syringes used for manual titrations.

» O2k-Protocols
» Product: Oroboros O2k, O2k-Catalogue

O2k-Network Lab: AT_Innsbruck_Oroboros

Labels: MiParea: Instruments;methods 

Regulation: ADP, Inhibitor, Substrate, Uncoupler, Amino acid 

HRR: O2k-Protocol 

O2k-chemicals and media 

Note: Titration of chemicals in respirometry

Overshoots or short interruptions of the flux after the injection of chemicals do not have a meaning in respirometric measurements. A disturbance of the signal is only observed due to the injected chemical and does not represent mitochondrial respiration. With the addition of a chemical solved in ethanol a little bit of oxygen is always introduced, since ethanol has higher oxygen solubility than water.

Further information

» MitoPedia: SUIT - Substrate-uncoupler-inhibitor-titration (SUIT) protocols for respirometry
» MiPNet03.02 - Chemicals and media for respirometry
» MiPNet19.14 - SOP for manual O2k-titrations with Hamilton microsyringes.