MiPNet09.12 Manual titrations of SUIT chemicals
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Oroboros (2024-11-25) Mitochondr Physiol Network
Abstract: Oroboros (2024) Manual titrations of SUIT chemicals with 2.0 mL and 0.5 mL chambers of the Oroboros Bioenergetics Analyzer. Mitochondr Physiol Network 09.12(21):1-4.
- Open the pdf document above.
- » Current Oroboros series: NextGen-O2k Series XB and O2k Series J
- » Current software versions DatLab 8.2: MitoPedia: DatLab
- Further details: » Oroboros Open Support
- » O2k-Protocols
- » Product: Oroboros
• O2k-Network Lab: AT_Innsbruck_Oroboros
Note
- Oligomycin: titrations should be performed by 1-2 µL steps (i.e. 5-10 nM experimental concentration of each step). Careful titration of oligomycin is necessary to determine the optimal inhibitory concentration (from 10 to 40 nM) as elaborated in Vernerova et al 2021 and Zdrazilova et al 2022 and to prevent inhibition of ET respiration as detailed in Ruas et al (2016) and Doerrier et al 2018.
Labels: MiParea: Instruments;methods
Regulation: ADP, Inhibitor, Substrate, Uncoupler, Amino acid
HRR: O2k-Protocol
O2k-chemicals and media, ADP
