Abstract: Oroboros (2019) O2k manual titrations: SUIT protocols with mitochondrial preparations. Mitochondr Physiol Network 09.12(19): 1-4.
A list of substrates, uncouplers, and inhibitors used in SUIT protocols in high-resolution respirometry, with information on abbreviations, stock concentrations, final experimental concentrations, volumes titrated into the 2 mL O2k-chamber and 0.5 mL O2k-Chamber sV (O2k-sV-Module), and syringes used for manual titrations.
• O2k-Network Lab: AT_Innsbruck_Oroboros
Labels: MiParea: Instruments;methods
Regulation: ADP, Inhibitor, Substrate, Uncoupler, Amino acid
O2k-chemicals and media
Note: Titration of chemicals in respirometry
- Overshoots or short interruptions of the flux after the injection of chemicals do not have a meaning in respirometric measurements. A disturbance of the signal is only observed due to the injected chemical and does not represent mitochondrial respiration. With the addition of a chemical solved in ethanol a little bit of oxygen is always introduced, since ethanol has higher oxygen solubility than water.