Difference between revisions of "SUIT-023 O2 ce D053"
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{{MitoPedia | {{MitoPedia | ||
|abbr= | |abbr=AOX-ce SHAM+CN | ||
|description=[[File:ce1;ce2SHAM;ce3KCN.png|350px]] | |description=[[File:ce1;ce2SHAM;ce3KCN.png|350px]] | ||
|info='''A''' Determination of the respiration due to the cytochrome c pathway -'''[[SUIT-023]]''' | |info='''A''' Determination of the respiration due to the cytochrome ''c'' oxidase pathway -'''[[SUIT-023]]''' | ||
|application=O2 | |application=O2 | ||
|SUIT number=D053_ce1;ce2SHAM;ce3KCN | |SUIT number=D053_ce1;ce2SHAM;ce3KCN | ||
}} | }} | ||
{{Template:SUIT text D053}} | |||
__TOC__ | |||
Communicated by [[Huete-Ortega M]] and [[Gnaiger E]] (last update 2021-03-23) | |||
== Representative traces == | |||
[[File:D053 O2 traces.png|600px]] | |||
{{Template:SUIT- | {{Template:SUIT-023 O2 ce D053}} | ||
== Strengths and limitations == | == Strengths and limitations == | ||
:::* SUIT-023 O2 ce D053 provides an estimation of the [[cyanide| cyanide]]-and [[Antimycin_A| antimycin A]]-non-resistant mitochondrial respiration | :::* SUIT-023 O2 ce D053 provides an estimation of the [[cyanide| cyanide]]-and [[Antimycin_A| antimycin A]]-non-resistant mitochondrial respiration in [[microalgal]] living cells. | ||
::: | :::* The concentrations of inhibitors used in this protocol have been optimised for the specific case of the microalgal model species ''Chlamydomonas reinhardtii'' | ||
:::+ Short duration of experiment | :::+ Short duration of experiment | ||
:::- | :::- No estimation of coupling control | ||
:::- The use of [[cyanide| cyanide]] requires | :::- The use of [[cyanide| cyanide]] requires careful cleaning of the chambers after use and careful handling of the chemical to guarantee health safety. | ||
== Compare SUIT protocols == | == Compare SUIT protocols == | ||
::::* [[SUIT- | ::::* [[SUIT-022 O2 ce D051]] has to be used in parallel to obtain complete information of the contribution of the Q-AOX and CIII-CIV pathways to mitochondrial respiration. | ||
== Chemicals and syringes == | |||
{{Template:Chemicals SUIT-023}} | |||
::: Suggested stock concentrations are shown in the specific DL-Protocol. | |||
Latest revision as of 12:45, 23 March 2021
Description
Abbreviation: AOX-ce SHAM+CN
Reference: A Determination of the respiration due to the cytochrome c oxidase pathway -SUIT-023
SUIT number: D053_ce1;ce2SHAM;ce3KCN
O2k-Application: O2
The SUIT-023 O2 ce D053 provides an estimation of mitochondrial respiration in microalgal living cells through the CIII-cytochrome c oxidase pathway (CIII-CIV), which is inhibited by antimycin A and cyanide. This protocol must be complemented with the study in parallel of the mitochondrial respiration through the Q-alternative oxidase pathway (see SUIT-022).
Communicated by Huete-Ortega M and Gnaiger E (last update 2021-03-23)
Representative traces
Steps and respiratory states
Step | State | Pathway | Q-junction | Comment - Events (E) and Marks (M) |
---|---|---|---|---|
ce1 | ROUTINE | ce1
| ||
ce2SHAM | ROUTINE OR ROX | ce1;ce2SHAM
| ||
ce3KCN | ROX | ce1;ce2SHAM;ce3KCN
|
- Bioblast links: SUIT protocols - >>>>>>> - Click on [Expand] or [Collapse] - >>>>>>>
- Coupling control
- Pathway control
- Main fuel substrates
- » Glutamate, G
- » Glycerophosphate, Gp
- » Malate, M
- » Octanoylcarnitine, Oct
- » Pyruvate, P
- » Succinate, S
- Main fuel substrates
- Glossary
Strengths and limitations
- SUIT-023 O2 ce D053 provides an estimation of the cyanide-and antimycin A-non-resistant mitochondrial respiration in microalgal living cells.
- The concentrations of inhibitors used in this protocol have been optimised for the specific case of the microalgal model species Chlamydomonas reinhardtii
- + Short duration of experiment
- - No estimation of coupling control
- - The use of cyanide requires careful cleaning of the chambers after use and careful handling of the chemical to guarantee health safety.
Compare SUIT protocols
- SUIT-022 O2 ce D051 has to be used in parallel to obtain complete information of the contribution of the Q-AOX and CIII-CIV pathways to mitochondrial respiration.
Chemicals and syringes
Step | Chemical(s) and link(s) | Comments |
---|---|---|
ce2SHAM | Salicylhydroxamic acid (SHAM) | |
ce3KCN | Cyanide (KCN) |
- Suggested stock concentrations are shown in the specific DL-Protocol.
References
MitoPedia concepts: SUIT protocol, SUIT A, Find
MitoPedia methods:
Respirometry