- high-resolution terminology - matching measurements at high-resolution
SUIT-006 O2 mt D022
Description
Abbreviation: S
Reference: A
SUIT number: D022_1SRot;2D;2c;3U;4Ama
O2k-Application: O2
MitoPedia concepts:
SUIT protocol,
SUIT A,
Find
MitoPedia methods:
Respirometry
Categories
- SUIT-category: S
- SUIT protocol pattern: linear coupling control protocol 1SRot;2D;2c;3U;4Ama
References
Steps and respiratory states
Step
|
State
|
Pathway
|
Q-junction
|
Comment - Events (E) and Marks (M)
|
1SRot
|
SL
|
S
|
CII
|
1SRot
|
2D
|
SP
|
S
|
CII
|
1SRot;2D
|
2c
|
ScP
|
S
|
CII
|
1SRot;2D;2c
|
(3Omy)
|
ScL(Omy)
|
|
|
1SRot;2D;2c;(3Omy)
- Omy addition is skipped in SUIT-006 O2 mt D022
- Succinate, S ( type S-pathway to Q).
- Non-phosphorylating resting state (LEAK state); LEAK-respiration, L(Omy), after blocking the ATP synthase with oligomycin.
|
4U
|
SE
|
S
|
CII
|
1SRot;2D;2c;4U
|
5Ama
|
ROX
|
|
|
1SRot;2D;2c;4U;5Ama
- Rox is the residual oxygen consumption in the ROX state, due to oxidative side reactions, estimated after addition of antimycin A (inhibitor of CIII). Rox is subtracted from oxygen flux as a baseline for all respiratory states, to obtain mitochondrial respiration (mt).
|
- Bioblast links: SUIT protocols - >>>>>>> - Click on [Expand] or [Collapse] - >>>>>>>
- Coupling control
- » Coupling control state
- » ET capacity
- » OXPHOS capacity
- » LEAK respiration
- Pathway control
- » Electron transfer pathway
- » Fatty acid oxidation pathway control state, F
- » NADH electron transfer-pathway state, N
- » Succinate pathway control state, S
- » NS-pathway control state, NS
- » Glycerophosphate pathway control state, Gp
- » Complex IV single step, CIV
- » Anaplerotic pathway control state
- Main fuel substrates
- » Glutamate, G
- » Glycerophosphate, Gp
- » Malate, M
- » Octanoylcarnitine, Oct
- » Pyruvate, P
- » Succinate, S
- Glossary
- » List of SUIT states
- » SUIT concept