Description
Abbreviation: ceGlc
Reference: A Coupling control protocol and Crabtree effect test -SUIT-003
SUIT number: D038_ce1;ce2U;ce3Glc
O2k-Application: O2
- MitoPedia: SUIT
- SUIT-category: ceGlc
- SUIT protocol pattern: complex cell coupling control protocol ce1;ce2U;ce3Glc
SUIT-003 O2 ce D038 has been designed to study the coupling control state and respiration regulation by glucose (Crabtree effect) of intact cells on ET-capacity. The different capacities are tested in the sequence OXPHOS and ET. SUIT-003 O2 ce D038 follows a modular approach and can be easily used in combination with the assessment of complex IV capacity and respirometric cell viability test.
Steps and respiratory states
Step | State | Pathway | Q-junction | Comment - Events (E) and Marks (M) |
---|---|---|---|---|
ce1 | ROUTINE | ce1
| ||
(ce2Omy) | L(Omy) | ce1;(ce2Omy)
| ||
ce3U | E | ce1;(ce2Omy);ce3U
| ||
ce3Glc | E | ce1;(ce2Omy);ce3U;ce3Glc
| ||
ce3'U | E | ce1;(ce2Omy);ce3U;ce3Glc;ce3'U
| ||
ce4Ama | ROX | ce1;(ce2Omy);ce3U;ce3Glc;ce3'U;ce4Ama
|
- Bioblast links: SUIT protocols - >>>>>>> - Click on [Expand] or [Collapse] - >>>>>>>
- Coupling control
- Pathway control
- Main fuel substrates
- » Glutamate, G
- » Glycerophosphate, Gp
- » Malate, M
- » Octanoylcarnitine, Oct
- » Pyruvate, P
- » Succinate, S
- Main fuel substrates
- Glossary
Strengths and limitations
- The SUIT-003 O2 ce D038 is a cell coupling control protocol. The total cell count (Nce) is the sum of viable cells (Nvce) and dead cells (Ndce).
- This protocol can be extended with the cell viability test module (See CCV SUIT-003 O2 ce-pce D020).
- CIV activity can also be measured after the cell viability test module.
- + Reasonable duration of the experiment.
- - Cell culture media with glucose (e.g. DMEM: 25 µM Glucose) could hinder the cell viability test.
- - Careful washing is required after the experiment to avoid carry-over of inhibitors and uncoupler.
Compare SUIT protocols
References
MitoPedia concepts: SUIT protocol, SUIT A, Find
MitoPedia methods:
Respirometry