Template:SUIT-006: Difference between revisions
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[[Image:SUIT-MitoFit.png|right|190px|link=http://www.bioblast.at/index.php/MitoPedia:_SUIT |MitoPedia: SUIT]] | [[Image:SUIT-MitoFit.png|right|190px|link=http://www.bioblast.at/index.php/MitoPedia:_SUIT |MitoPedia: SUIT]] | ||
== Steps and respiratory states == | == Steps and respiratory states == | ||
[[File: | [[File:1X;2D;2c;3Omy;4U;5Ama.png|450px]] | ||
{| class="wikitable" border="1" | {| class="wikitable" border="1" | ||
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|- | |- | ||
| | | 1X | ||
| [[X]]<sub>[[LEAK respiration |''L''(n)]]</sub> ย | |||
| ย | | ย | ||
| ย | | ย | ||
| | | 1X | ||
* X: combination of substrates and inhibitors chosen according to the aims. ย | |||
*{{Template:SUIT L(n)}} | |||
{{Template:SUIT | |- | ||
| 2D | |||
|ย [[X]]<sub>''[[OXPHOS|P]]''</sub> | |||
| | |||
| | |||
| 1X;2D | |||
ย | |||
*{{Template:SUIT OXPHOS}} | |||
ย | |||
|- | |||
| 2c | |||
| [[X]]c<sub>''[[OXPHOS|P]]''</sub> | |||
| | |||
| | |||
| 1X;2D;2c | |||
ย | |||
*{{Template:SUIT OXPHOS}} | |||
*{{Template:SUIT c}} | |||
|- | |- | ||
| | | 3Omy | ||
| | | [[X]]<sub>[[LEAK respiration |''L''Omy]]</sub> | ||
| ย | | ย | ||
| ย | | ย | ||
| | | 1X;2D;2c;3Omy | ||
{{Template:SUIT | *{{Template:SUIT L(Omy)}} ย | ||
*The addition of [[oligomycin]] is optional depending on the experimental question to resolve. If it is known that ''L''<sub>n</sub> has the same values for ''L''<sub>Omy</sub> for a kind of sample and substrates, this step may be skipped. If the carrier of oligomycin (EtOH) is used instead, it is possible to evaluate whether it affects OXPHOS. In these cases, LEAK state is not reached again. | |||
|- | |- | ||
| | | 4U | ||
| E | | [[X]]<sub>''[[E]]''</sub> | ||
| ย | | ย | ||
| ย | | ย | ||
| | | 1X;2D;2c;3Omy;4U | ||
{{Template:SUIT U*}} | *{{Template:SUIT U*}} | ||
|- | |- | ||
| | | 5Ama | ||
| [[ROX]] | | [[ROX]] | ||
| ย | | ย | ||
| | | ย | ||
| {{Template:SUIT Ama}} | | 1X;2D;2c;3Omy;4U;5Ama | ||
ย | |||
*{{Template:SUIT Ama}} | |||
ย | |||
|} | |} | ||
{{Template:Keywords in SUIT protocols}} | {{Template:Keywords in SUIT protocols}} |
Latest revision as of 13:01, 25 November 2020
Steps and respiratory states
Step | State | Pathway | Q-junction | Comment - Events (E) and Marks (M) |
---|---|---|---|---|
1X | XL(n) | 1X
| ||
2D | XP | 1X;2D
| ||
2c | XcP | 1X;2D;2c
| ||
3Omy | XLOmy | 1X;2D;2c;3Omy
| ||
4U | XE | 1X;2D;2c;3Omy;4U
| ||
5Ama | ROX | 1X;2D;2c;3Omy;4U;5Ama
|
- Bioblast links: SUIT protocols - >>>>>>> - Click on [Expand] or [Collapse] - >>>>>>>
- Coupling control
- Pathway control
- ยป Electron transfer pathway
- ยป Fatty acid oxidation pathway control state, F
- ยป NADH electron transfer-pathway state, N
- ยป Succinate pathway control state, S
- ยป NS-pathway control state, NS
- ยป Glycerophosphate pathway control state, Gp
- ยป Complex IV single step, CIV
- ยป Anaplerotic pathway control state
- Pathway control
- Main fuel substrates
- ยป Glutamate, G
- ยป Glycerophosphate, Gp
- ยป Malate, M
- ยป Octanoylcarnitine, Oct
- ยป Pyruvate, P
- ยป Succinate, S
- Main fuel substrates
- Glossary