Oroboros Ecosystem Agenda 2020 Archive: Difference between revisions
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:::: [[Image:O2k-Support.jpg|60px|link=http://wiki.oroboros.at/index.php/O2k-Open_Support |O2k-Open Support]] [[File:Yellow-take care.png|40px|Take care]] '''Do you know that you can use the [[TIP2k-Module|Titration-Injection microPump (TIP2k)]] to perform an instrumental O<sub>2</sub> background test | :::: [[Image:O2k-Support.jpg|60px|link=http://wiki.oroboros.at/index.php/O2k-Open_Support |O2k-Open Support]] [[File:Yellow-take care.png|40px|Take care]] '''Do you know that you can use the [[TIP2k-Module|Titration-Injection microPump (TIP2k)]] to perform an instrumental O<sub>2</sub> background test with the [[O2k-sV-Module]]?''' | ||
::::::: '''(1) By adjusting the | ::::::: '''(1) By adjusting the dithionite concentration to 2.5 mM''', the instrumental O<sub>2</sub> background test can be performed with the "BG Feedback" settings. - »'''[[Dithionite|Dithionite]]'''« <br> | ||
::::::: '''(2) By adjusting the TIP2k setup with specific settings for the O2k-sV-Module''', the instrumental O<sub>2</sub> background test can be performed using the O2k-sV-Module. - »'''[[MiPNet24.14 O2k-sV-Module manual|O2k-sV-Module]]'''« | ::::::: '''(2) By adjusting the TIP2k setup with specific settings for the O2k-sV-Module''', the instrumental O<sub>2</sub> background test can be performed using the O2k-sV-Module. - »'''[[MiPNet24.14 O2k-sV-Module manual|O2k-sV-Module Manual]]'''« | ||
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Revision as of 09:10, 29 May 2020
Oroboros Ecosystem Agenda 2020 Archive
- Mitochondria and Cell Research - the Oroboros Ecosystem
May 2020
2020 Week 21
- May-18 Mo
Did you know that a humid O2k-environment and/or liquid spillage may lead to a high raw POS signal [V]?
See how to clean and dry the electrical connections of the OroboPOS-Connector.
- »Cleaning the electrical connections«
- May-19 Tu
Allergic rhinitis induces mitochondrial dysfunction in peripheral blood cells«
- from the O2k-Network »Charles Anne-Laure« »Geny Bernard«
- Qi S, Barnig C, Charles AL, Poirot A, Meyer A, Clere-Jehl R, de Blay F and Geny Bernard
(2017) Nasal allergen challenge in allergic rhinitis induces mitochondrial dysfunction of peripheral blood mononuclear cells. Ann Allergy Asthma Immunol 10.1016/j.anai.2016.11.026. »Bioblast link«
- May-20 We
SI units
One year ago, on World Metrology Day (2019-May-20) the redefinition of the SI units came into force. Science faced one of the largest overhauls in history of scientific units. How to commemorate this event of groundbreaking innovation better than with the online publication of Mitochondrial physiology and with the launch of an innovative journal — Bioenergetics Communications.
MitoEAGLE Consortium Communication: Mitochondrial physiology ― Published online: 2020-05-20. Gnaiger E et al ― MitoEAGLE Task Group (2020) Mitochondrial physiology. Bioenerg Commun 2020.1
» https://www.bioenergetics-communications.org/index.php/BEC2020.1_doi10.26124bec2020-0001.v1
- May-21 Th
As part of the H2020 SME NextGen-O2k project we are developing a PhotoBiology module that will allow one to study the effect of different light intensities on O2 consumption and production.
-»PhotoBiology«
- May-22 Fr
2020 Week 20
- May-11 Mo
Do you know how to avoid underestimating/overestimating your respiratory fluxes? Measuring flux at steady states with high-resolution respirometry is the key to obtaining correct values.
Learn more about steady states including performance and mark setting for data analysis
- »Steady state«
- May-12 Tu
Mitochondria - a driving force in adipocyte differentiation
- from the O2k-Network »CZ Pilsen Kuncova J«
- Kladnická I, Čedíková M, Kripnerová M, Dvořáková J, Kohoutová M, Tůma Z, Müllerová D, Kuncová J (2019) Mitochondrial respiration of adipocytes differentiating from human mesenchymal stem cells derived from adipose tissue. Physiol Res 68:287-96. »Bioblast link«
- from the O2k-Network »CZ Pilsen Kuncova J«
- May-13 We
- May-14 Th
How can I measure ATP production with the O2k?
Learn more about how to measure ATP production in mitochondrial preparations with Magnesium Green
- »Magnesium Green«
- May-15 Fr
Restoration of mitochondrial oxygen consumption and decrease of mitochondrial ROS by AOX in the post-ischaemic heart are not sufficient to confer acute or chronic cardioprotection in mouse
- from the O2k-Network » FI Helsinki Jacobs HT « , » DE Magdeburg Gellerich FN « , » FI Tampere Dufour E « , » DE Magdeburg Debska-Vielhaber G « and » DE Frankfurt Wittig I«
- Szibor Marten, Schreckenberg Rolf, Gizatullina Zemfira, Dufour Eric, Wiesnet Marion, Dhandapani Praveen Kumar, Debska-Vielhaber Grazyna, Heidler Juliana, Wittig Ilka, Nyman Tuula A, Gaertner Ulrich, Hall Andrew R, Pell Victoria, Viscomi Carlo, Krieg Thomas, Murphy Michael P, Braun Thomas, Gellerich Frank Norbert, Schlueter Klaus-Dieter, Jacobs Howard T(2020) Respiratory chain signalling is essential for adaptive remodelling following cardiac ischaemia. J Cell Mol Med [Epub ahead of print]. - »Bioblast link«
- from the O2k-Network » FI Helsinki Jacobs HT « , » DE Magdeburg Gellerich FN « , » FI Tampere Dufour E « , » DE Magdeburg Debska-Vielhaber G « and » DE Frankfurt Wittig I«
- May-17 Su World Hypertension Day
Mitochondrial DNA enhances the hypoxia-induced cardioprotective effect in hypertensive rats
- from the O2k-Network »CZ Prague Houstek J« and »CZ Prague Kalous M«
- Neckar J, Svatonova A, Weissova R, Drahota Z, Zajickova P, Brabcova I, Kolar D, Alanova P, Vasinova J, Silhavy J, Hlavackova M, Tauchmannova K, Milerova M, Ostadal B, Cervenka L, Zurmanova J, Kalous M, Novakova O, Novotny J, Pravenec M, Kolar F (2017) Selective replacement of mitochondrial DNA increases the cardioprotective effect of chronic continuous hypoxia in spontaneously hypertensive rats. Clin Sci (Lond) 131(9):865-881. »Bioblast link«
2020 Week 19
- May-04 Mo
Do you load the correct instrumental background correction according to the actual range of experimental O2 concentrations?
Perform and apply the instrumental background test:
- » O2 background correction in DatLab« and » Instrumental oxygen background«
- May-05 Tu
- Expression of alternative oxidase prevents reverse electron transfer
- - from the O2k-Network »FI Helsinki Jacobs HT« »DE Magdeburg Gellerich FN« »FI Tampere Dufour E« »DE Magdeburg Debska-Vielhaber G« »DE Frankfurt Wittig I« and »UK Brighton Moore AL«
- Szibor Marten, Gainutdinov Timur, Fernandez-Vizarra Erika, Dufour Eric, Gizatullina Zemfira, Debska-Vielhaber Grazyna, Heidler Juliana, Wittig Ilka, Viscomi Carlo, Gellerich Frank Norbert, Moore Anthony L (2019) Bioenergetic consequences from xenotopic expression of a tunicate AOX in mouse mitochondria: switch from RET and ROS to FET. Biochim Biophys Acta Bioenerg 1861:148137. - »Bioblast link«
- Expression of alternative oxidase prevents reverse electron transfer
- May-06 We
- May-07 Th
Quantify fatty acid oxidation capacity (FAO, F-pathway), avoiding a common artefact of overestimation. Malate anaplerotic pathways (see mt-malic enzyme) are active in various tissues and cultures cells, which requires caution: »Malate anaplerotic pathway control state« - DatLab 7.4 provides a step-by-step guide to evaluate the malate anaplerotic pathway - »SUIT-027«
- Since F-pathway capacity can be overestimated due to anaplerotic reactions, a reference protocol was designed to avoid this artefact - »SUIT-002«
- May-08 Fr
Apr 2020
2020 Week 18
- Apr-27 Mo
- Do you set marks correctly for instrumental O2 background correction when planning to apply them to a SUIT protocol with a high O2 regime?
- The range of O2 concentration selected in the instrumental background test should correspond to the range of experimental O2 concentration. - » O2 Background correction - troubleshooting«
- Do you set marks correctly for instrumental O2 background correction when planning to apply them to a SUIT protocol with a high O2 regime?
- Apr-28 Tu
- Cytosolic calcium controls extramitochondrial pyruvate generation and mitochondrial pyruvate uptake
- - from the O2k-Network »FI Helsinki Jacobs HT«, »DE Magdeburg Gellerich FN« and »DE Magdeburg Debska-Vielhaber G«
- Szibor Marten, Gizatullina Zemfira, Gainutdinov Timur, Endres Thomas, Debska-Vielhaber Grazyna, Kunz Matthias, Karavasili Niki, Hallmann Kerstin, Schreiber Frank, Bamberger Alexandra, Schwarzer Michael, Doenst Torsten, Heinze Hans-Jochen, Leßmann Volkmar, Vielhaber Stefan, Kunz Wolfram S, Gellerich Frank Norbert (2020) Cytosolic, but not matrix, calcium is essential for adjustment of mitochondrial pyruvate supply. J Biol Chem [Epub ahead of print]. - »Bioblast link«
- Cytosolic calcium controls extramitochondrial pyruvate generation and mitochondrial pyruvate uptake
- Apr-29 We
- Apr-30 Th
- May-01 Fr
2020 Week 17
- Apr-20 Mo
- - from the O2k-Network »US TN Nashville Wasserman DH« and »US MI East Lansing Bazil JN«
- Wollenman LC, Vander Ploeg MR, Miller ML, Zhang Y, Bazil JN (2017) The effect of respiration buffer composition on mitochondrial metabolism and function. PLOS ONE 12:e0187523. - »Bioblast link«
- Did you know that some substrates alone cannot maintain a stable mitochondrial respiratory flux in OXPHOS?
- Unstable respiratory fluxes in OXPHOS might be due to substrates limitation - » Pyruvate - troubleshooting«
- Did you know that some substrates alone cannot maintain a stable mitochondrial respiratory flux in OXPHOS?
- Apr-21 Tu
Citrate synthase activity is commonly applied as a mitochondrial marker - and erroneous applications are common in the literature.
- Apr-22 We
- Apr-23 Th
- Apr-24 Fr
ETC - the electron transport chain - is the most frequently applied misnomer in bioenergetics. Y Hatefi in the team of DE Green clarified this 58 years ago, introducing the concept of the electron transfer system.
2020 Week 16
- Apr-13 Mo
You can quantify the ATPase activity in your mitochondrial preparation: - DatLab 7.4 provides a step-by-step guide through the SUIT-024 protocol of mitochondrial preparations - »SUIT-024«
- Apr-14 Tu
- Why are the oxygen levels different between chambers prior to my experimental run?
- After switching on the O2k, air calibration has to be performed; otherwise the displayed concentrations may deviate from accurate concentrations - » Oxygen calibration - troubleshooting«
- Why are the oxygen levels different between chambers prior to my experimental run?
- Apr-15 We
- Apr-16 Th
- - from the O2k-Network »DE Frankfurt Eckert GP« »CH Basel Eckert A«
- Leuner K, Schulz K, Schütt T, Pantel J, Prvulovic D, Rhein V, Savaskan E, Czech C, Eckert A, Müller WE (2012) Peripheral mitochondrial dysfunction in Alzheimer's disease: focus on lymphocytes. Mol Neurobiol 46:194-204. - »Bioblast link«
- Apr-17 Fr
2020 Week 15
- Apr-06 Mo
- The world of humankind is and will be different during and after the COVID-19 crisis – a fact beyond our competence. How different the world will be – depends on our ability to learn and communicate.
- Physical activity has positive effects on mitochondria from patients with occupational burnout
- - from the O2k-Network »CH Basel Eckert A«
- Brand S, Ebner K, Mikoteit T, Lejri I, Gerber M, Beck J, Holsboer-Trachsler E, Eckert A (2020) Influence of regular physical activity on mitochondrial activity and symptoms of burnout - an interventional pilot study. J Clin Med 9:E667. - »Bioblast link«
- Physical activity has positive effects on mitochondria from patients with occupational burnout
- Apr-07 Tu
- If you did not apply a finally evaluated O2 background correction to your experimental DatLab file, you can edit the O2 background parameters directly in the O2 analysis template DL7.4 for completion of Oxygen flux analysis - DatLab 7.4. - »SUIT: Browse DL-Protocols and templates«
- Apr-08 We
- Apr-09 Th
- Apr-10 Fr
2020 Week 14
- Mar-30 Mo
- DatLab 7.4 offers the flexibility to edit a DL-Protocol: »Enable DL-Protocol editing«
- Mar-31 Tu
- Apr-01 We
- Parkinson’s Disease Awareness Month: Dominant mutation in Parkinson’s Disease causes loss of protein function in mitochondria
- - from the O2k-Network »FR Angers Gueguen N« and »BE Leuven Spinazzi M«
- Cornelissen T, Spinazzi M, Martin S, Imberechts D, Vangheluwe P, Bird M, De Strooper B, Vandenberghe W (2020) CHCHD2 harboring the Parkinson's disease-linked T61I mutation precipitates inside mitochondria and induces precipitation of wild-type CHCHD2. Hum Mol Genet [Epub ahead of print]. »Bioblast link«
- Parkinson’s Disease Awareness Month: Dominant mutation in Parkinson’s Disease causes loss of protein function in mitochondria
- Apr-02 Th
- Do you want to search the best SUIT protocol for your research questions? Use the SUITbrowser: »SUITbrowser«
- Creative solutions coming from the O2k-Network labs - » NZ Auckland Hickey AJ
- Apr-03 Fr
- - from the O2k-Network »DE Nuthetal Klaus S«
- Ost M, Igual Gil C, Coleman V, Keipert S, Efstathiou S, Vidic V, Weyers M, Klaus S (2020) Muscle-derived GDF15 drives diurnal anorexia and systemic metabolic remodeling during mitochondrial stress. EMBO Rep [Epub ahead of print]. - »Bioblast link«
Mar 2020
2020 Week 13
- Mar-23 Mo
- Do you know that you can use the Titration-Injection microPump (TIP2k) to perform an instrumental O2 background test with the O2k-sV-Module?
- (1) By adjusting the dithionite concentration to 2.5 mM, the instrumental O2 background test can be performed with the "BG Feedback" settings. - »Dithionite«
- (2) By adjusting the TIP2k setup with specific settings for the O2k-sV-Module, the instrumental O2 background test can be performed using the O2k-sV-Module. - »O2k-sV-Module Manual«
- (1) By adjusting the dithionite concentration to 2.5 mM, the instrumental O2 background test can be performed with the "BG Feedback" settings. - »Dithionite«
- Do you know that you can use the Titration-Injection microPump (TIP2k) to perform an instrumental O2 background test with the O2k-sV-Module?
- Mar-24 Tu
- Tuberculosis Day: Mitochondria as target to Mycobacterium tuberculosis infection
- - from the O2k-Network »US NC Durham Li PA«
- Lu X, Williams Z, Hards K, Tang J, Cheung CY, Aung HL, Wang B, Liu Z, Hu X, Lenaerts AJ, Woolhiser L, Hastings C, Zhang X, Wang Z, Rhee KY, Ding K, Zhang T, Cook GM (2018) A pyrazolo[1,5-a]pyridine inhibitor of the respiratory cytochrome bcc complex for the treatment of drug-resistant tuberculosis. ACS Infect Dis 5:239-49. - »Bioblast link«
- Tuberculosis Day: Mitochondria as target to Mycobacterium tuberculosis infection
- Mar-25 We
- Preprint: An urgent call for research on COVID-19 and public vitamin D awareness
- Brown RA, Sarkar A (2020) Vitamin D deficiency: a factor in COVID-19, progression, severity and mortality? – An urgent call for research. MitoFit Preprint Arch doi:10.26124/mitofit:200001.
- Preprint: An urgent call for research on COVID-19 and public vitamin D awareness
- Research findings made available via preprint servers before journal publication - Sharing research data and findings relevant to the novel coronavirus (COVID-19) outbreak
- O2 flux analysis with DatLab 7.4. Use our latest O2 analysis template DL7.4: »O2k-Videosupport: O2 flux analysis with DatLab 7.4«
- Mar-26 Th
- Mar-27 Fr
2020 Week 12
- Mar-16 Mo
- Oroboros operates from now on in home office mode - we stay connected.
- The standardized coupling control protocol to explore the bioenergetics of living cells
- - DatLab 7.4 provides a step-by-step guide through the SUIT-003 protocol and its extensions. - »SUIT-003«
- The standardized coupling control protocol to explore the bioenergetics of living cells
- Mar-17 Tu
- Zika virus infection leads to mitochondrial dysfunction
- - from the O2k-Network »BR Rio de Janeiro Galina A«
- Ledur PF, Karmirian K, Pedrosa CDSG, Souza LRQ, Assis-de-Lemos G, Martins TM, Ferreira JCCG, de Azevedo Reis GF, Silva ES, Silva D, Salerno JA, Ornelas IM, Devalle S, Madeiro da Costa RF, Goto-Silva L, Higa LM, Melo A, Tanuri A, Chimelli L, Murata MM, Garcez PP, Filippi-Chiela EC, Galina A, Borges HL, Rehen SK (2020) Zika virus infection leads to mitochondrial failure, oxidative stress and DNA damage in human iPSC-derived astrocytes. Sci Rep 10:1218. - »Bioblast link«
- Zika virus infection leads to mitochondrial dysfunction
- Mar-18 We
- - from the O2k-Network »KR Busan Han J« »KR Incheon Kwak HB«
- No MH, Heo JW, Yoo SZ, Kim CJ, Park DH, Kang JH, Seo DY, Han J, Kwak HB (2020) Effects of aging and exercise training on mitochondrial function and apoptosis in the rat heart. Pflugers Arch 472:179-93. - »Bioblast link«
- Mar-19 Th
- Home office mode is a world-wide protective strategy facing the COVID-19 pandemic. Supporting institutions and individual scientists in their home office strategy, during the time of crisis Oroboros Instruments provides free of charge copies of our newest software DatLab 7.4, for downloading at your personal or institutional PC at home.
- - » For the download link and password, please email [email protected]
- Home office mode is a world-wide protective strategy facing the COVID-19 pandemic. Supporting institutions and individual scientists in their home office strategy, during the time of crisis Oroboros Instruments provides free of charge copies of our newest software DatLab 7.4, for downloading at your personal or institutional PC at home.
- Mar-20 Fr
- Abstract submission deadline - »SHVM 2020 Halifax CA«
2020 Week 11
- Mar-08 Su - International Women's Day
- Promising practices for addressing the underrepresentation of women in science, engineering, and medicine: opening doors. National Academies of Sciences, Engineering, and Medicine (2020) - »Bioblast link«
- Mar-09 Mo
- »MitoEAGLE WG2 Innsbruck AT« (Mar 09-13)
- Protein-specific mitochondrial respiratory capacity is higher in women than men
- - from the O2k-Network »SE Stockholm Cardinale DA«
- Cardinale DA, Larsen FJ, Schiffer TA, Morales-Alamo D, Ekblom B, Calbet JAL, Holmberg HC, Boushel R (2018) Superior intrinsic mitochondrial respiration in women than in men. Front Physiol 9:1133. - »Bioblast link«
- Protein-specific mitochondrial respiratory capacity is higher in women than men
- Mar-10 Tu
- Muscular exercise for training adipose tissue mitochondria
- - from the O2k-Network »DK Copenhagen Larsen S«
- Mendham Amy E, Larsen Steen, George Cindy, Adams Kevin, Hauksson Jon, Olsson Tommy, Fortuin-de Smidt Melony C, Nono Nankam Pamela A, Hakim Olah, Goff Louise M, Pheiffer Carmen, Goedecke Julia H (2020) Exercise training results in depot-specific adaptations to adipose tissue mitochondrial function. Sci Rep 10:3785. - »Bioblast link«
- Muscular exercise for training adipose tissue mitochondria
- Mar-11 We
- Does blebbistatin reduce oxygen dependence of respiration of permeabilized muscle fibers? This is one of the questions addressed in the COST Action CA15203 MitoEAGLE WG2. - »Blebbistatin«
- Let's talk COVID-19 - a new culture of communication in science. - Kay Kupferschmidt in Science
- Do you change the slope smoothing to 20 while operating the O2k with biological sample?
- - It is advantageous to decrease 'slope smoothing' to visualize changes of respiration more rapidly in O2k-experiments after calibration - especially before uncoupler titrations. - »Slope smoothing«
- Do you change the slope smoothing to 20 while operating the O2k with biological sample?
- Mar-12 Th
- Multiple Sclerosis Month: Mitochondrial permeability transition pore protection as novel therapeutic approach in multiple sclerosis
- - from the O2k-Network »UK London Duchen MR«
- Warne J, Pryce G, Hill J, Shi X, Lennerås F, Puentes F, Kip M, Hilditch L, Walker P, Simone MI, Chan AW, Towers GJ, Coker A, Duchen MR, Szabadkai G, Baker D, Selwood D (2015) Selective inhibition of the mitochondrial permeability transition pore protects against neuro-degeneration in experimental multiple sclerosis. J Biol Chem 291:4356-73. - »Bioblast link«
- Multiple Sclerosis Month: Mitochondrial permeability transition pore protection as novel therapeutic approach in multiple sclerosis
- Mar-13 Fr
- Men and women with Type 1 diabetes and skeletal muscle mitochondria
- - from the O2k-Network »CA Toronto Perry CG«
- Monaco CMF, Bellissimo CA, Hughes MC, Ramos SV, Laham R, Perry CGR, Hawke TJ (2020) Sexual dimorphism in human skeletal muscle mitochondrial bioenergetics in response to type 1 diabetes. Am J Physiol Endocrinol Metab 318:E44-E51. - »Bioblast link«
- Men and women with Type 1 diabetes and skeletal muscle mitochondria
2020 Week 10
- Mar-02 Mo
- An amazing number of new O2k-Publications from China. - »O2k-Network Labs China«
- Salty diet, cardiac hypertrophy, calcium overload, and mitochondrial dysfunction
- - from the O2k-Network »CN Chongqing Zhu Z«
- Lu Z, Cui Y, Wei X, Gao P, Zhang H, Wei X, Li Q, Sun F, Yan Z, Zheng H, Yang G, Liu D, Zhu Z (2018) Deficiency of PKD2L1 (TRPP3) exacerbates pathological cardiac hypertrophy by augmenting NCX1-mediated mitochondrial calcium overload. Cell Rep 24:1639-52. - »Bioblast link«
- Salty diet, cardiac hypertrophy, calcium overload, and mitochondrial dysfunction
- Mar-03 Tu
- Rapamycin suppresses senescence in galactose versus glucose cell culture
- - from the O2k-Network »US PA Philadelphia Orynbayeva Z«
- Nacarelli T, Azar A, Altinok O, Orynbayeva Z, Sell C (2018) Rapamycin increases oxidative metabolism and enhances metabolic flexibility in human cardiac fibroblasts. Geroscience 40:243-56. - »Bioblast link«
- Rapamycin suppresses senescence in galactose versus glucose cell culture
- Rapamycin - more differences or more in common with other mitObesity drugs? - »Rapamycin«
- Advancing Open Science practices: stakeholder perspectives on incentives and disincentives. National Academies of Sciences, Engineering, and Medicine (2020) - »Bioblast link« - The concept of Preprints needs full attention in Open Science.
- Mar-04 We
- Systemic mitochondrial bioenergetics in older adults - skeletal muscle and platelets
- - from the O2k-Network »US FL Orlando Goodpaster BH«
- Braganza A, Corey CG, Santanasto AJ, Distefano G, Coen PM, Glynn NW, Nouraie SM, Goodpaster BH, Newman AB, Shiva S (2019) Platelet bioenergetics correlate with muscle energetics and are altered in older adults. JCI Insight 5:128248. - »Bioblast link«
- Systemic mitochondrial bioenergetics in older adults - skeletal muscle and platelets
- Sugar and fat - initial mitochondrial aging in the brain
- - from the O2k-Network »IT Naples Iossa S«
- Crescenzo R, Spagnuolo MS, Cancelliere R, Iannotta L, Mazzoli A, Gatto C, Iossa S, Cigliano L (2019) Effect of initial aging and high-fat/high-fructose diet on mitochondrial bioenergetics and oxidative status in rat brain. Mol Neurobiol 56:7651-63. - »Bioblast link«
- Sugar and fat - initial mitochondrial aging in the brain
- A damaged OroboPOS-Holder may cause medium to leak from the O2k-Chamber.
- See how you can check that the two blue OroboPOS-Holders are intact. - »O2k-OroboPOS-Holder«
- A damaged OroboPOS-Holder may cause medium to leak from the O2k-Chamber.
- Mar-05 Th
- Abstract submission opens - »Europhysiology 2020 Berlin DE«
- With a preprint you can communicate recent findings fast to the scientific community, secure a DOI, and receive input from other scientists even before submitting to a peer-reviewed journal. - »Preprint«
- Mar-06 Fr
Feb 2020
2020 Week 9
- Feb-23 Su
- » 2020 PaduaMuscleDays Padova IT - postponed to fall/winter 2020 due to coronavirus outbreak in Northern Italy
- Feb-24 Mo
- More research is needed on the impact of viral infection on mitochondrial function. - »Viruses and mitochondrial medicine«
- Feb-25 Tu
- High quality mitochondrial research is increasing rapidly in South Korea. - »O2k-Network Labs Republic of Korea«
- An active lifestyle protects cardiac mitochondria
- - from the O2k-Network: KR Busan Han J, KR Incheon Kwak HB
- No MH, Heo JW, Yoo SZ, Kim CJ, Park DH, Kang JH, Seo DY, Han J, Kwak HB (2020) Effects of aging and exercise training on mitochondrial function and apoptosis in the rat heart. Pflugers Arch 472:179-93. - »Bioblast link«
- An active lifestyle protects cardiac mitochondria
- Feb-26 We
- It is essential to titrate uncouplers in multiple steps for respirometric measurement of electron transfer capacity. - »Noncoupled respiration«
- MitoEAGLE WG4 Innsbruck AT (Feb 26-28)
- Feb-27 Th
- Regulation of mitochondrial oxygen kinetics by cytochrome c oxidase
- - from the O2k-Network: CZ Prague Houstek J
- Pajuelo-Reguera David, Čunátová Kristýna, Vrbacký Marek, Pecinová Alena, Houštěk Josef, Mráček Tomáš, Pecina Petr (2020) Cytochrome c oxidase subunit 4 isoform exchange results in modulation of oxygen affinity. Cells 9:E443. - »Bioblast link«
- Regulation of mitochondrial oxygen kinetics by cytochrome c oxidase
- Feb-28 Fr
- Calibration parameters are transferred to experimental DatLab files
- Save calibration information from selected Marks while DatLab is connected to the O2k during an instrumental calibration run. Air calibration and zero oxygen calibration can be performed and saved in a single DatLab file or in separate DatLab files. - » Oxygen calibration - DatLab«
- Calibration parameters are transferred to experimental DatLab files
2020 Week 8
- Feb-17 Mo
- Registration is open: European Bioenergetics Conference 2020, Marseille, France, 2020
- Feb-18 Tu
- Why do we fight gas bubbles? » Dive deep into Oxygen solubility
- Aging and hyperlipidemia impair aortic mitochondrial function and increase the risk of atherosclerosis
- - from the O2k-Network »US MI Ann Arbor Beard DA«
- Tyrrell DJ, Blin M, Song J, Wood S, Zhang M, Beard DA, Goldstein D (2019) Age-associated mitochondrial dysfunction accelerates atherogenesis. Circ Res 126:298–314. - »Bioblast link«
- Aging and hyperlipidemia impair aortic mitochondrial function and increase the risk of atherosclerosis
- Feb-19 We
- - »MitoFit Preprint Arch« was initiated one year ago. We invite submissions of mitochondrial preprints.
- Spermidine and other calorie restriction mimetics control autophagy and mitochondrial function. - »Spermidine«
- Feb-20 Th
- - MitoFit Open Seminar: Eduard Stefan: “Decoding and Perturbation of ONCO-Kinase functions” and Omar Torres Quesada: “MitoKIN - Systematic barcoding of kinase-controlled mitochondrial function”
2020 Week 7
- Feb-10 Mo
- Early Bird Registration Deadline for ESSR 2020 Innsbruck AT
- Feb-12 We
- What do metformin, melatonin and other bioactive mitObesity compounds have in common? - »MitObesity drugs«
- I had a very pleasant experience at the work shop. I thank you and all the team members for devoting their time for us and teaching each step in a systematic manner. - Gayathri Narayanappa participaing in MiPNet25.03 IOC144 Innsbruck AT
- Feb-13 Th
2020 Week 6
- Feb-03 Mo
- »Official start of the FFG funded project »MitoKIN«
- Blood cell mitochondrial function does not provide a general substitute for muscle biopsies
- - from the O2k-Network »US AR Little Rock Boersheim E«
- Rose S, Carvalho E, Diaz EC, Cotter M, Bennuri SC, Azhar G, Frye RE, Adams SH, Boersheim E (2019) A comparative study of mitochondrial respiration in circulating blood cells and skeletal muscle fibers in women. Am J Physiol Endocrinol Metab 317:E503-E512. - »Bioblast link«
- Blood cell mitochondrial function does not provide a general substitute for muscle biopsies
- Do you know that the Oroboros O2k can also be used to study the mitochondrial dark respiration of microalgae and its response to climate change impact in the ocean? One of our customers, Dr Nelly Tremblay is using for the first time the SUIT-022 protocol to study alternative oxidases in the marine model organism Phaeodactylum tricornutum.
- Feb-04 Tu
- MiPNet25.10 IOC and Distributor engagement Tokyo JP (Feb 4-7)
- Cronin (2018) The metabolite BH4 controls T cell proliferation in autoimmunity and cancer. Nature - Lymphocytes, Cancer - O2k-brief repository
- Feb-05 We
- Aerobic and resistance training address central defects and peripheral comorbidities in Alzheimer’s disease
- - from the O2k-Network »US FL Boca Raton Khamoui AV«
- Pena GS, Paez HG, Johnson TK, Halle JL, Carzoli JP, Visavadiya NP, Zourdos MC, Whitehurst MA, Khamoui AV (2020) Hippocampal growth factor and myokine cathepsin B expression following aerobic and resistance training in 3xTg-AD mice. Int J Chronic Diseases 2020:Article ID 5919501. - »Bioblast link«
- Aerobic and resistance training address central defects and peripheral comorbidities in Alzheimer’s disease
- Feb-06 Th
- MitoFit Open Seminar
- 16:00-16:30 Jorge Beleza: Exercise performed during pregnancy as a preventive tool against transgenerational development of diabetes mellitus and NAFLD in the offspring. The role of epigenetic-mediated mechanisms. »Abstract«
- 16:30-17:00 Rita Pereira: The Handball4Health Project : A community-based intervention programme
- MitoFit Open Seminar
Jan 2020
2020 Week 5
- Jan-27 Mo
- IOC145 Innsbruck AT - O2k-Coaching Days (Jan 27-29)
- DSL-Seminar, 16:00
- Gnaiger E: mitObesity: compromised mitochondrial fitness in degenerative comorbidities of early aging.
- Univ.-Klinik für Visceral-, Transplantations- u. Thoraxchirurgie, Surgery building, 12th floor, north, seminar room
- DSL-Seminar, 16:00
- Jan-28 Tu
- Mitochondrial respiration of blood cells and cardiovascular disease
- - from the O2k-Network »FR Strasbourg Zoll J«
- Alfatni A, Riou M, Charles AL, Meyer A, Barnig C, Andres E, Lejay A, Talha S, Geny B (2020) Peripheral blood mononuclear cells and platelets mitochondrial dysfunction, oxidative stress, and circulating mtDNA in cardiovascular diseases. J Clin Med 9 pii:E311. - »Bioblast link«
- Mitochondrial respiration of blood cells and cardiovascular disease
- Jan-30 Th - MitoFit Open Seminar - Alexander Karabatsiakis: Targeting the reversibility of mitochondrial changes in bioenergetics and biogenesis in Major Depressive Disorder by clinical routine antidepressant treatment.
- Do you check the O2 flux at the end of the air calibration with the O2k-chamber closed?
- In DatLab 7.4, instrumental DL-Protocols guide you automatically towards closing the O2k-chamber after air calibration, to monitor "O2 slope neg." (negative) as a test of any biological contamination which would increase oxygen consumption in the absence of a biological sample. - »Oxygen calibration - DatLab«
- Do you check the O2 flux at the end of the air calibration with the O2k-chamber closed?
- Your air calibration is not effective until you set mark ‘R1’ andclick on Calibrate and copy to clipboard in the O2 calibration window. - »Oxygen calibration - DatLab«
- Open Innovation in the NextGen-O2k software development DatLab 8. Here is a summary of MitoPedia pages including DatLab 8 perspectives
- Jan-31 Fr
- Deadline for APS2020 Abstract submission for poster presentation.
- Call for EJTM Communications - deadline for manuscript submissions
- Registration deadline for 6th EU-Cardioprotection Meeting 2020 Riga LV
- Reproducibility committment in high-resolution respirometry: quality control in oxygen calibration
- In the DatLab 7.4 software, new instrumental DL-Protocols guide the user automatically step-by-step, to calibrate the instrument accurately. Reproducibility is monitored and documented when following user-friendly standard operating procedures (SOP). - »Oxygen calibration - DatLab«
- Reproducibility committment in high-resolution respirometry: quality control in oxygen calibration
2020 Week 4
- Jan-20 Mo
- IOC144 Innsbruck AT - O2k-Coaching Days (Jan 20-22)
- Jan-21 Tu
- Mr Hai Wu (CEO of Huawei, Oroboros distributor in China) and Dr. Rupert Ecker (CEO of Vienna TissueGnostics) join the O2k-Coaching Days for extending collaborative projects.
- Jan-23 Th - MitoFit Open Seminar - Erich Gnaiger: An improved index of obesity versus BMI - an issue of mitObesity and gender medicine in our modern world's obesogenic environment.
- Jan-24 Fr
- Further circulars to invited speakers for submission of tentative titles for oral and poster presentations - MitoEAGLE Summit Obergurgl 2020
- Do you write the serial number of the polarographic oxygen sensor (POS) in the ‘O2k configuration’ window?
- Including the POS serial number in the ´O2k configuration´ window is important to keep a track record of the sensors used according to the O2k. This will be especially helpful if troubleshooting is needed. - »O2k configuration«
- Do you write the serial number of the polarographic oxygen sensor (POS) in the ‘O2k configuration’ window?
- Do you perform O2k-Chamber volume calibration?
- The volume calibration is essential to ensure that the chamber volume is correctly calibrated to its standard volume of 2 mL (or 0.5 mL for the O2k-sV-Module). - »O2k-Chamber MitoPedia«
- Do you perform O2k-Chamber volume calibration?
- Do you use the correct O2 solubility factor (FM) in the ‘O2 calibration’ window in DatLab?
- The O2 solubility factor varies according to the medium used and influences the air calibration. - »Oxygen solubility factor«
- Do you use the correct O2 solubility factor (FM) in the ‘O2 calibration’ window in DatLab?
2020 Week 3
- Jan-14 Tu
- Jan-15 We
- Statistical evaluation on replica in HRR with permeabilized skeletal muscle fibers
- - from the O2k-Network »AU Melbourne Stepto NK«
- Jacques M, Kuang J, Bishop DJ, Yan X, Alvarez-Romero J, Munson F, Garnham A, Papadimitriou I, Voisin S, Eynon N (2019) Mitochondrial respiration variability and simulations in human skeletal muscle: The Gene SMART study. FASEB J [Epub ahead of print]. - »Bioblast link«
- Statistical evaluation on replica in HRR with permeabilized skeletal muscle fibers
- Jan-16 Th
- Oxygen flux - which SI units should be used?
- Did you ever think that units for body mass could be an issue? - »Body mass«
- Jan-17 Fr
- HRR - high-resolution respirometry and oxygen kinetics 10 years ago: Cell respiration under hypoxia: facts and artefacts in mitochondrial oxygen kinetics. Adv Exp Med Biol 2010. - »Bioblast link«
- TRANSMIT Symposium 2020 Brussels BE (Jan 17-18)
- Mitochondrial respiration is oxygen-dependent in human cardiac homogenate preparations
- - from the O2k-Network »CZ Prague Krajcova A«
- Krajčová A, Urban T, Megvinet D, Waldauf P, Balík M, Hlavička J, Budera P, Janoušek L, Pokorná E, Duška F (2020) High resolution respirometry to assess function of mitochondria in native homogenates of human heart muscle. PLOS ONE 15:e0226142. - »Bioblast link«
- Mitochondrial respiration is oxygen-dependent in human cardiac homogenate preparations
2020 Week 2
- Jan-07 Tu The Oroboros Office opens in the New Year 2020
- From the Austrian Bioenergetics Group to Mitochondrial Physiology and Bioenergetics DACH (D: Germany; A: Austria; CH: Switzerland)
- EBEC22 - the 22nd European Bioenergetics Conference will be held in Innsbruck (2022, Aug 22-27). We would like to invite several Austrian colleagues to join the EBEC22 National Scientific Committee. This provides an excellent opportunity to establish an Austrian Bioenergetics Group towards and beyond EBEC22. We hope that this topic will be discussed at the forthcoming 1st Myocardial Function Symposium 2020 Graz AT, and further at the MitoEAGLE Summit Obergurgl 2020. Please, contact the organizer of EBEC22 - [email protected]
- Jan-08 We
- Jan-09 Th
- Planning the MitoEAGLE Summit Obergurgl 2020
- Members of the Management Committee MitoEAGLE and Working Group Leaders: please submit your suggestions for MitoEAGLE Summit Obergurgl 2020:
- Suggested type and title of your personal presentation, according to the MoU and final MitoEAGLE output
- Suggested scientists (titles) for presentations, according to the MoU
- Members of the Management Committee MitoEAGLE and Working Group Leaders: please submit your suggestions for MitoEAGLE Summit Obergurgl 2020:
- Jan-10 Fr
- » 1st Myocardial Function Symposium 2020 Graz AT (Jan 10-11)
- 14:45 Novel technologies for mitochondria and cell research (Gnaiger, Innsbruck, AUT)
- » 1st Myocardial Function Symposium 2020 Graz AT (Jan 10-11)
- Jan-12 So
- One month ago
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2020 Week 1 The Oroboros team enjoys holidays from 2019 Dec 24 to 2020 Jan 06
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- Jan-04 Sa
- The Oroboros Ecosystem Agenda 2020 is launched as a signature of the Oroboros Ecosystem.
- One month ago
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