Step
|
State
|
Pathway
|
Q-junction
|
Comment - Events (E) and Marks (M)
|
1PM
|
PML(n)
|
N
|
CI
|
1PM
|
2D
|
PMP
|
N
|
CI
|
1PM;2D
|
2c
|
PMcP
|
N
|
CI
|
1PM;2D;2c
- NADH-linked substrates (type N-pathway to Q).
- OXPHOS capacity, P (with saturating [ADP]), active OXPHOS state.
- Addition of cytochrome c yields a test for integrity of the mtOM. Stimulation by added cytochrome c would indicate an injury of the mtOM and limitation of respiration in the preceding state without added c due to loss of cytochrome c. Typically, cytochrome c is added immediately after the earliest ADP-activation step (P, OXPHOS-capacity with saturating [ADP]).
|
3U
|
PME
|
N
|
CI
|
1PM;2D;3U
|
4G
|
PGME
|
N
|
CI
|
1PM;2D;3U;4G
|
5S
|
PGMSE
|
NS
|
CI&II
|
1PM;2D;3U;4G;5S
- Respiratory stimulation by simultaneous action of type N substrates & succinate, with convergent electron flow in the NS-pathway for reconstitution of TCA cycle function.
- Noncoupled electron transfer state, ET-state, with ET-capacity, E.
|
6Oct
|
OctPGMSE
|
FNS
|
FAO&CI&II
|
1PM;2D;3U;4G;5S;6Oct
- Respiratory stimulation by simultaneous action of fatty acid (F), type N substrates (N), and succinate (S), with convergent electron flow in the FNS-pathway for reconstitution of TCA cycle function and additive or inhibitory effect of F.
- Noncoupled electron transfer state, ET-state, with ET-capacity, E.
|
7Rot
|
SE
|
S
|
CII
|
1PM;2D;3U;4G;5S;6Oct;7Rot
|
8Gp
|
SGpE
|
SGp
|
CII&GpDH
|
1PM;2D;3U;4G;5S;6Oct;7Rot;8Gp
- Respiratory stimulation by action of succinate and glycerophosphate, Gp, with convergent electron flow in the SGp-pathway (CII&GpDH-linked pathway to the Q-junction).
- Noncoupled electron transfer state, ET-state, with ET-capacity, E.
|
9Ama
|
ROX
|
|
|
1PM;2D;3U;4G;5S;6Oct;7Rot;8Gp;9Ama
- Rox is the residual oxygen consumption in the ROX state, due to oxidative side reactions, estimated after addition of Antimycin A (inhibitor of CIII). Rox is subtracted from oxygen flux as a baseline for all respiratory states, to obtain mitochondrial respiration (mt).
|