Template:SUIT-027: Difference between revisions

Latest revision as of 12:03, 3 June 2020

Step	State	Pathway	Q-junction	Comment - Events (E) and Marks (M)
1M.05	(M_L)			ce1;1Dig;1M Low concentration of malate, typically low concentration, does not saturate the N-pathway, and will allow us to find the minimum concentration to stimulate the anaplerosis.
2D	( M_P)			ce1;1Dig;1M;2D
3M	M_P	N	CI	ce1;1Dig;1M;2D;3M NADH-linked substrates (type N-pathway to Q). Malate titration in the presence of saturating [ADP] allows the evaluation of malate anaplerotic pathways.
4P	PM_P	N	CI	ce1;1Dig;1M;2D;3M;4P NADH-linked substrates (type N-pathway to Q). OXPHOS capacity P (with saturating [ADP]), active OXPHOS state.
5G	PGM_P	N	CI	ce1;1Dig;1M;2D;3M;4P;5G NADH-linked substrates (type N-pathway to Q). OXPHOS capacity P (with saturating [ADP]), active OXPHOS state.
6Ama	ROX			ce1;1Dig;1M;2D;3M;4P;5G;6Ama Rox is the residual oxygen consumption in the ROX state, due to oxidative side reactions, estimated after addition of antimycin A (inhibitor of CIII). Rox is subtracted from oxygen flux as a baseline for all respiratory states, to obtain mitochondrial respiration (mt).

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Coupling control

» Coupling control state

Pathway control

» Electron transfer pathway

Main fuel substrates

» Glutamate, G

Glossary

» List of SUIT states

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 [[Image:SUIT-MitoFit.png|right|190px|link=http://www.bioblast.at/index.php/MitoPedia:_SUIT |MitoPedia: SUIT]]
 == Steps and respiratory states ==
-[[File:1M;2D;3M;4P;5G-.png|400px]]
+[[File:1M;2D;3M;4P;5G;6Ama.png|400px]]
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 | 1M.05
-| ([[Malate anaplerotic pathway control state|M]]<sub>[[LEAK|''L'']]</sub>)
+| ([[Malate-anaplerotic pathway control state|M]]<sub>[[LEAK|''L'']]</sub>)
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 | 2D
-| ([[Malate anaplerotic pathway control state| M]]<sub>[[OXPHOS|''P'']]</sub>)
+| ([[Malate-anaplerotic pathway control state| M]]<sub>[[OXPHOS|''P'']]</sub>)
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 | ce1;1Dig;1M;2D
-*{{Template:SUIT OXPHOS}}
 |-
 | 3M
-| [[Malate anaplerotic pathway control state| M]]<sub>[[OXPHOS|''P'']]</sub>
+| [[Malate-anaplerotic pathway control state| M]]<sub>[[OXPHOS|''P'']]</sub>
 | [[N]]
 | CI
 | ce1;1Dig;1M;2D;3M
 *{{Template:SUIT N}}
-*{{Template:SUIT OXPHOS}}
+*Malate titration in the presence of saturating [ADP] allows the evaluation of malate anaplerotic pathways.
 |-
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 *{{Template:SUIT Ama}}
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+{{Keywords: SUIT protocols}}
-{{Template:Keywords in SUIT protocols}}