Template:SUIT-003 O2 ce-pce D020
Steps and respiratory states
Step | State | Pathway | Q-junction | Comment - Events (E) and Marks (M) |
---|---|---|---|---|
ce5S | ce1;ce2Omy;ce3U;ce4Rot;ce5S
Succinate, S ( type S-pathway to Q). | |||
1Dig* | SE | S | CII | ce1;ce2Omy;ce3U;ce4Rot;ce5S;1Dig
Multiple Digitonin titrations for complete plasma membrane permeabilization. Noncoupled electron transfer state, ET state, with ET capacity E. |
1c | SE | S | CII | ce1;ce2Omy;ce3U;ce4Rot;ce5S;1Dig;1U;1c
Addition of cytochrome c yields a test for integrity of the mtOM (cytochrome c control efficiency). Stimulation by added cytochrome c would indicate an injury of the mtOM and limitation of respiration in the preceding state without added c due to loss of cytochrome c. Typically, cytochrome c is added immediately after the earliest ADP-activation step (OXPHOS capacity P with saturating [ADP]). Noncoupled electron transfer state, ET state, with ET capacity E. |
2Ama | ROX | ce1;ce2Omy;ce3U;ce4Rot;ce5S;1Dig;1U;1c;2Ama
Rox is the residual oxygen consumption in the ROX state, due to oxidative side reactions, estimated after addition of antimycin A (inhibitor of CIII). Rox is subtracted from oxygen flux as a baseline for all respiratory states, to obtain mitochondrial respiration (mt). Rox is the residual oxygen consumption in the ROX state, due to oxidative side reactions, estimated either after inhibition of CIII (e.g. antimycin A, myxothiazol), CIV (e.g. Cyanide) or in the absence of endogenous fuel-substrates. Rox is subtracted from oxygen flux as a baseline for all respiratory states, to obtain mitochondrial respiration. |
Step | Respiratory state | Pathway control | ET-Complex | Comment |
---|---|---|---|---|
## AsTm | AsTmE | CIV | CIV | |
## Azd | CHB |
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- Coupling control
- Pathway control
- Main fuel substrates
- » Glutamate, G
- » Glycerophosphate, Gp
- » Malate, M
- » Octanoylcarnitine, Oct
- » Pyruvate, P
- » Succinate, S
- Main fuel substrates
- Glossary