Difference between revisions of "Talk:MitoFit Proficiency Test"
From Bioblast
| (18 intermediate revisions by 4 users not shown) | |||
| Line 12: | Line 12: | ||
'''Chemicals:''' | '''Chemicals:''' | ||
::::* for [[SUIT-RP1]] | ::::* for [[SUIT-001_O2_ce-pce_D003 |SUIT-RP1]] and for [[SUIT-002 O2 ce-pce D007 |SUIT-RP2]]: | ||
:::: | ::::» [[Digitonin]], [[Pyruvate]], [[Malate]], [[ADP]], [[Cytochrome c]], [[CCCP]], [[Glutamate]], [[Succinate]], [[Octanoylcarnitine]], [[Rotenone]], [[Glycerophosphate]], [[Antimycin A]] | ||
:::: We are aware that the Quality Control of the chemicals is a key factor influencing the results of the MitoFit Proficiency test. For the present test, however, due to logistic limitations, participants are asked to perform tests using their own chemicals. For future tests, provision of critical chemicals is planned. Should the lack of some chemicals be the main reason why you do not see fit to participate in the current round, we kindly ask for your feedback and see if we can solve the issue. We will try to solve any problems. | |||
| Line 20: | Line 22: | ||
::::* '''individually''' (one O2k instrument per participant) | ::::* '''individually''' (one O2k instrument per participant) | ||
::::* without any further help | ::::* without any further help | ||
::::* Manuals and SOPs can be used, see: [[O2k- | ::::* Manuals and SOPs can be used, see: [[O2k-Open Support]] | ||
''' | ''' Registration form and self assessment:''' | ||
:::: | :::: [[File:PDF.jpg|100px|link=http://wiki.oroboros.at/index.php/File:Registration_form_MitoFit_Proficiency_test.pdf |MitoFit PT pdf]] | ||
:::: Send the registration form to [mailto:[email protected] [email protected]] | |||
== Time schedule == | == Time schedule == | ||
=== Round 01 - | === Round 01 - 20 labs invited === | ||
{| class="wikitable" | {| class="wikitable" | ||
| Line 43: | Line 44: | ||
|- | |- | ||
| 2018-10-12 to 15 | | 2018-10-12 to 15 | ||
| '''Level I''' | | '''Level I PT''' to be performed: instrumental background oxygen flux and air calibration, using the Instrumental DLPs | ||
Shipping of HEK cells | |||
|- | |- | ||
| 2018-10- | | 2018-10-16 | ||
| DatLab Files have to be sent to [mailto:[email protected] [email protected]] | | DatLab Files have to be sent to [mailto:[email protected] [email protected]] | ||
|- | |- | ||
| 2018-10-17 | | 2018-10-17 | ||
| Line 53: | Line 54: | ||
Feedback on Level I sent to participants | Feedback on Level I sent to participants | ||
|- | |- | ||
| 2018-10- | | 2018-10-18 to 26 | ||
| '''Level II''' | | '''Level II PT''' to be performed: SUIT RP 1/2, technical repeats | ||
|- | |- | ||
| 2018-10- | | 2018-10-29 | ||
| DatLab files to be sent to Oroboros for analysis (Level II) | | DatLab files to be sent to Oroboros for analysis (Level II) | ||
|- | |- | ||
| 2018- | | 2018-11-09 | ||
| | | Report sent to the participants | ||
|- | |- | ||
| Line 73: | Line 74: | ||
:::: '''Level 1.''' Air calibration and Instrumental background oxygen flux: ca. 3 hours | :::: '''Level 1.''' Air calibration and Instrumental background oxygen flux: ca. 3 hours | ||
:::: '''Level 2.''' SUIT- | :::: '''Level 2.''' [[SUIT-001]] or [[SUIT-002]]: ca. 3 hours, including air calibration | ||
:::: '''Level 3.''' Data analysis: 1 hour | :::: '''Level 3.''' Data analysis: 1 hour | ||
:::: Within the timespan of one workday, your personal proficiency is comprehensively evaluated, using your own O2k and your laboratory equipment. | :::: Within the timespan of one workday, your personal proficiency is comprehensively evaluated, using your own O2k and your laboratory equipment. | ||
| Line 83: | Line 84: | ||
::::* [[MitoPedia:_SUIT| MitoPedia - SUIT]] | ::::* [[MitoPedia:_SUIT| MitoPedia - SUIT]] | ||
::::* [[MiPNet19.18E_O2_flux_analysis_-_DatLab_6|Flux analysis]] | ::::* [[MiPNet19.18E_O2_flux_analysis_-_DatLab_6|Flux analysis]] | ||
::::* DatLab Protocols, DLP: List of titrations, marks, overview on the website. | ::::* [[Run_DL-Protocol/Set_O2_limit|DatLab Protocols, DLP: List of titrations, marks, overview on the website]]. | ||
Latest revision as of 16:52, 28 May 2019
Important information for the MitoFit Proficiency Test (PT)
Required equipment:
- Oroboros O2k, Series A-H (2004–2018)
- DatLab 7.3 (provided)
- PC/Laptop, Windows XP+
- O2k-Titration_Set with Hamilton microsyringes
Chemicals:
- We are aware that the Quality Control of the chemicals is a key factor influencing the results of the MitoFit Proficiency test. For the present test, however, due to logistic limitations, participants are asked to perform tests using their own chemicals. For future tests, provision of critical chemicals is planned. Should the lack of some chemicals be the main reason why you do not see fit to participate in the current round, we kindly ask for your feedback and see if we can solve the issue. We will try to solve any problems.
Experiments are to be performed:
- individually (one O2k instrument per participant)
- without any further help
- Manuals and SOPs can be used, see: O2k-Open Support
Registration form and self assessment:
- Send the registration form to [email protected]
Time schedule
Round 01 - 20 labs invited
| Date | |
|---|---|
| 2018-10-11 | DatLab 7.3 and instructions to be sent to the participants of the study |
| 2018-10-12 to 15 | Level I PT to be performed: instrumental background oxygen flux and air calibration, using the Instrumental DLPs
Shipping of HEK cells |
| 2018-10-16 | DatLab Files have to be sent to [email protected] |
| 2018-10-17 | Arrival of shipment at customer’s site at the latest
Feedback on Level I sent to participants |
| 2018-10-18 to 26 | Level II PT to be performed: SUIT RP 1/2, technical repeats |
| 2018-10-29 | DatLab files to be sent to Oroboros for analysis (Level II) |
| 2018-11-09 | Report sent to the participants |
Scientific objective
- Together with a literature review and preliminary data from the Oroboros MitoFit laboratory, we are going to publish the test results in collaboration with the Department for Medical Statistics, Informatics and Health Economics, Medical University of Innsbruck, as part of the K-Regio Project MitoFit.
- With the participation in the MitoFit Proficiency Test, you agree on the use of experimental data collected for the test, to be used for research purposes in an anonymized way.
Time requirements
- Level 1. Air calibration and Instrumental background oxygen flux: ca. 3 hours
- Level 2. SUIT-001 or SUIT-002: ca. 3 hours, including air calibration
- Level 3. Data analysis: 1 hour
- Within the timespan of one workday, your personal proficiency is comprehensively evaluated, using your own O2k and your laboratory equipment.
Additional support
- For a comprehensive list of DatLab menus: MitoPedia DatLab
- MitoPedia - SUIT
- Flux analysis
- DatLab Protocols, DLP: List of titrations, marks, overview on the website.
