O2k-Titration Set

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O2k-Titration Set

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O2k-Open Support

MitoPedia O2k and high-resolution respirometry: O2k-Open Support 


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The O2k-Titration Set consists of Hamilton microsyringes (6 x 10 mm3 and 3 spare plungers, 6 x 25 mm3, 1 x 50 mm3, 1 x 100 mm3, 1 x 500 mm3; fixed needles with rounded tips), provided in the Syringe Storage Box with Syringe Labels, a set of two Syringe Racks with Syringe Collars, and a set of two Tube Racks.

Reference: Product details and purchasing information

Additional resources

»MiPNet09.12 O2k-Titrations
»MiPNet19.14 SOP Hamilton microsyringes

Note: Titration of chemicals in respirometry

Overshoots or short interruptions of the flux after the injection of chemicals do not have a meaning in respirometric measurements. A disturbance of the signal is only observed due to the injected chemical and does not represent mitochondrial respiration. With the addition of a chemical solved in ethanol a little bit of oxygen is always introduced, since ethanol has higher oxygen solubility than water.

Further information

  • Safety information: fixed needles are used with rounded tips, not usable for injection into tissues.
  • Hamilton microsyringes are specifically produced for O2k-titrations and can be ordered directly from Oroboros Instruments to assure exact fitting with the O2k-Stopper and O2k-Chamber dimensions.
  • Visit also: Hamilton.
  • Eliminate rough edges with 3M™ Wetordry™ Paper Sheet P1000 by gentle rubbing.
  • Use a cleaning wire for the needle. If a syringe is clogged , sometimes it is also helpful to fill it from the back with EtOH or H2O and try to press the plunger gently down.

MitoPedia O2k and high-resolution respirometry: O2k-Open Support, O2k-Respirometry, O2k-FluoRespirometry