COST Action CA15203 (2016-2021): MitoEAGLE
Evolution-Age-Gender-Lifestyle-Environment: mitochondrial fitness mapping

MitoEAGLE data: muscle

WG2

MITOEAGLE data repository in muscle and other tissues

WG2 Project application

Description

During the past 10 years, a dramatically increasing number of studies report respirometric data on human muscle tissue (e.g. Christiansen 2015 Am J Physiol). Muscle mitochondrial function is particularly studied in genetically engineered mouse models of human diseases (e.g. Jelenik 2014 Diabetes). However, despite the many published data, lack of consistency often precludes quantitative comparison among data sets with permeabilized or homogenized muscle fibres or isolated mitochondria, normalized to either tissue wet weight, dry weight, protein content, citrate synthase activity or other mitochondrial markers. In the absence of validated conversion factors, these data remain largely unconnected and can only be qualitatively linked.

The aim of WG2 will be to provide access to complete data sets for post-study statistical analysis and use harmonization tools (WG1) for standardized documentation, to initiate a data repository on muscle tissue from humans and model organisms in health and disease. Data sets shall be obtained from already published and new studies, prompting participating labs to report experimental details beyond those already published as far as possible (using tools such as PubMed Commons). As a result, specific protocols will be summarized for handling muscle tissue. Depending on specific interests, WG2 may decide to extend their focus to other tissue types.

Management

Tasks

Call for participating labs to deliver relevant experimental data on already published studies and to report them in a standardized format.
Collection and indexing of the data sets as required for statistical analysis.
Discussion of procedures, protocols and general guidelines.
Draft a comprehensive review on mt-function in muscle tissue (registration at PROSPERO, link with EQUATOR).
Opening of the database to the research public for data search and moderated entry of new data sets.

Milestones

Call for data report delivered.
Data sets entered in standardized format.
Summary, interpretation and discussion of data for review.

Deliverables

A MITOEAGLE database on muscle tissue from humans and model organisms.
A set of guidelines for future studies on mitochondrial bioenergetics in muscle tissue.
Draft of review manuscript.

WG2 Action

WG Leader

Pablo Miguel Garcia-Roves, PhD. - [email protected]

Participants

Country	City	Contact
Australia	Melbourne	Bishop David J
Austria	Innsbruck	Gnaiger Erich
Austria	Salzburg	Mayr JA
Austria	Innsbruck	Doerrier Carolina
Austria	Innsbruck	Laner Verena
Belgium	Leuven	Bird Matthew
Belgium	Liege	Votion Dominique-Marie
Brazil	Jaboticabal, SP	Garcia Geovana S
Brazil	São Paulo	Ferreira Julio Cesar B
Canada	Rimouski	Vandermeeren E
Canada	Vancouver	Boushel Robert C
Canada	Edmonton (Alberta)	Lemieux Helene
Canada	Rimouski	Blier Pierre U
Canada	Antigonish	Kane Daniel A
Croatia	Zagreb	Saric A
Croatia	Split	Mihanovic I
Czech Republic	Pilsen	Kuncova J
Czech Republic	Hradec Králové	Waldauf P
Czech Republic	Pilsen	Markova Michaela
Czech Republic	Prague	Elkalaf Moustafa
Czech Republic	Prague 10	Krajcova Adela
Denmark	Hvidovre	Nehlin Jan O
Denmark	Copenhagen	Gonzalez-Franquesa Alba
Denmark	Malov	Hey-Mogensen M
Denmark	Copenhagen	Dela Flemming
Denmark	Copenhagen N	Mortensen OH
Denmark	Copenhagen	Larsen Steen
Estonia	Tallinn	Puurand Marju
Estonia	Tallinn	Truu Laura
Estonia	Tallinn	Kaambre Tuuli
Estonia	Tallinn	Tepp Kersti
Estonia	Tallinn	Aid-Vanakova Jekaterina
Estonia	Tallinn	Vendelin Marko
Estonia	Tallinn	Karro N
Estonia	Tartu	Paju Kalju
Finland	Helsinki	Pirinen E
Finland	Jyvaeskylae	Lehti Maarit
France	Paris	Armand Anne-Sophie
France	Paris	Petit Patrice X
France	Bordeaux cedex	Mourier A
France	Grenoble Cedex 9	Schlattner Uwe
France	Montpellier Cedex 1	Chabi Beatrice
France	Grenoble cedex 9	Dubouchaud Herve
France	Paris	Ventura-Clapier R
France	Montpellier	Cortade F
Germany	Duesseldorf	Roden Michael
Germany	Köln	Kimoloi Sammy
Germany	Düsseldorf	Granata Cesare
Germany	Duesseldorf	Jelenik T
Germany	Bremerhaven	Mark Felix Christopher
Germany	Leipzig	Ost Mario
Germany	Cologne	Pesta Dominik
Germany	Rostock	Haider F
Germany	Nuthetal	Klaus S
Germany	Munich	Jastroch M
Germany	Bochum	Platen P
Greece	Heraklio	Sidiropoulou K
Greece	Athens	Trougakos Ioannis P
Greece	Heraklion	Tavernarakis Nektarios
India	Haryana	Sharma Vipin
Ireland	Sligo	Murphy J
Ireland	Dublin	O'Gorman Donal
Ireland	Dublin	Porter Richard K
Israel	Rishon Le Zion	Hachmo Yafit
Israel	Zrifin	Sova Marina
Israel	Jerusalem	Saada Reisch Ann
Italia	Udine	Salvadego Desy
Italy	Milan	Giovarelli Matteo
Italy	Bologna	Genova Maria Luisa
Italy	Pisa	Gastaldelli Amalia
Italy	Novara	Reano Simone
Italy	Rome	Scatena Roberto
Italy	Milan	De Palma Clara
Japan	Sapporo	Shirakawa R
Japan	Sapporo	Nambu H
Japan	Sapporo	Maekawa S
Latvia	Riga	Liepins Edgars
Latvia	Riga	Kuka Janis
Latvia	Riga	Zvejniece Liga
Latvia	Riga	Inashkina I
Luxembourg	Strassen	Devaux Yvan
Malta	Msida	Vella Joanna
Netherlands	Amsterdam	De Goede Paul
Netherlands	Maastricht	Hoeks Joris
Netherlands	BT Amsterdam	Jaspers Richard T
Netherlands	Maastricht	Grevendonk L
Netherlands	Wageningen	Keijer Jaap
Netherlands	Maastricht	Van Polanen N
Niederlande	ER Masstricht	De Wit-Verheggen V
Niederlande	ER Masstricht	De Theije C
Norway	Tromsoe	Aasum E
Norway	Oslo	Torp May-Kristin
Norway	Oslo	Nikolic N
Norway	Tromsoe	Jansen Kirsten M
Norway	Oslo	Rustan AC
Norway	Oslo	Eide L
Norway	Bergen	Tronstad Karl Johan
Norway	Tromsoe	Larsen Terje S
Norway	Tromsoe	Schots Pauke C
Norway	Ullevål	Heiestad Christina
Norway	Oslo	Lund Jenny
Poland	Lodz	Watala Cezary
Poland	Lodz	Labieniec-Watala Magdalena
Poland	Warsaw	Sek Aleksandra
Poland	Warsaw	Kampa Rafal Pawel
Poland	Warsaw	Dymkowska Dorota
Portugal	Coimbra	Pereira Susana P
Portugal	Coimbra	Carvalho Eugenia
Portugal	Porto	Beleza Jorge
Portugal	Cantanhede	Martins Joao D
Portugal	Cantanhede	Ferreira LL
Portugal	Coimbra	Martins Marques T
Portugal	Coimbra	Palmeira Carlos
Portugal	Porto	Coxito Pedro
Portugal	Porto	Magalhaes Jose
Romania	Timisoara	Lighezan R
Romania	Timisoara	Muntean Danina M
Singapore	Singapore	Hausenloy Derek J
Slovakia	Bratislava	Ukropec J
Slovakia	Bratislava	Sumbalova Zuzana
Slovakia	Bratislava	Ukropcova B
Slovakia	Bratislava	Maderova D
Slovakia	Bratislava	Nemec Michal
Slovenia	Ljubljana	Mars T
Slovenia	Ljubljana	Pirkmajer Sergej
Slovenia	Ljubljana	Dolinar K
South Africa		Smith James
Spain	Barcelona	Batlle M
Spain	Armilla (Granada)	Fernandez-Ortiz Marisol
Spain	Armilla (Granada)	Acuna-Castroviejo Dario
Spain	Barcelona	Gama Perez Pau
Spain	Armilla, Granada	Escames Germaine
Spain	A Coruña	Blanco FJ
Spain	Barcelona	Garcia-Roves Pablo Miguel
Spain	Barcelona	Guitart M
Spain	Granada	Lopez Garcia Luis Carlos
Spain	Madrid	Cadenas S
Spain	Madrid	Moran M
Spain	Barcelona	Sebastian D
Spain	Barcelona	De la Torre Lara J
Spain	Sevilla	Bustos Matilde
Spain	Barcelona	Zorzano Antonio
Spain	Barcelona	Catalan M
Sweden	Linköping	Fontes-Oliveira CC
Sweden	Stockholm	Cardinale Daniele A
Switzerland	Lausanne	Morato Fornaguera L
Switzerland	Bern	Nuoffer Jean-Marc
Switzerland	Lausanne	Sandi Carmen
Switzerland	Lausanne	Weger M
Switzerland	Lausanne	Zalachoras I
Switzerland	Lausanne	Gebara E
The Netherlands	Wageningen	Van der Ende Miranda
The Netherlands	BT Amsterdam	Wuest Rob CI
Turkey	Ankara	Tuncay Erkan
USA	Ann Arbor	Beard Daniel A
USA	Orlando	Distefano Giovanna
USA	Boca Raton	Khamoui Andy V
USA	Little Rock	Porter Craig
USA	St Louis	Welch R
USA	Baton Rouge	Axelrod Christopher L
USA	Gainesville	Wohlgemuth Stephanie E
USA	Gainesville	Neyroud D
USA	College Station	Latham C
USA	Chicago	Dayanidhi Sudarshan
USA	Orlando	Coen Paul M
USA	Baton Rouge	Irving Brian A
USA	Fairbanks	O'Brien Kristin
USA	Torrance	Rossiter Harry B
USA	Baltimore	Gonzalez-Freire Marta
USA	College Station	White-Springer Sarah H
USA	Fort Collins	Chicco Adam J
USA	Colorado Springs	Jacobs RA
USA	Norfolk	Lai Nicola
USA	Gainesville	Hepple Russell T
USA	Seattle	Marcinek DJ
USA	Baltimore	Fang Evandro F
United Kingdom	Birmingham	Joanisse S
United Kingdom	Manchester	Giakoumaki I
United Kingdom	London	Szabadkai Gyorgy
United Kingdom	Loughborough	Ebanks Brad
United Kingdom	Birmingham	Garten Antje
United Kingdom	Plymouth, Devon	McKenna Helen T
United Kingdom	Birmingham	Thakker A
United Kingdom	Leeds	Boyle JP
United Kingdom	Cambridge	Shiroma Jonathan G
United Kingdom	Brighton	Moore Anthony L
United Kingdom	Nottingham	Chakrabarti Lisa
United Kingdom	Cambridge	Murray Andrew James
United Kingdom	Birmingham	Ashcroft S
United Kingdom	Oxford	Makrecka-Kuka Marina
United States	Providence	Siamwala J

Task groups

TG leaders: suggestions discussed at the MitoFit Science Camp 2016 Kuehtai AT.

TG participants: listed as a summary from the feedback received.

TG2.1 Skeletal

TG leaders: Garcia-Roves Pablo M ES / Votion Dominique-Marie BE / Coen Paul M US

TG participants: Boyle John P UK, Chabi Beatrice FR, Garcia-Roves Pablo MES, Lehti Maarit FI, Mars Tomaz SI, Pirkmajer Sergej SI, Rustan Arild NO, Schlattner UweFR, Wuest Rob C NL

TG2.2 Cardiac

TG leaders: Larsen Terje S NO / Makrecka-Kuka Marina LV

TG participants: Muntean Danina M RO , Schlattner Uwe FR, Vendelin Marko EE, Wuest Rob C NL

Comments and discussion

Skeletal muscle is the main tissue we are studying in the lab. - Beatrice Chabi (2016).
For the kind of work group, our expertise is certainly most on muscle tissue (skeletal and heart), although we also worked on all kinds of permeabilized cells (primary cells and cell lines). For all these, we have extended data sets, mainly rat, but also human, from diverse experimental setups (nutritional regimen, exercise, drugs ...). - Uwe Schlattner (2016).
In my working group we are mainly interested in muscle, especially focusing on aging and sarcopenia (using animal models). I would be happy if new cooperations could be formed through the COST Action. I could imagine working on a joint review article. Next to examining muscle from mouse models, we are studying biopsies from human heart muscle and liver, but there is no published data on these studies yet. - Susanne Klaus (2016). Translated by Sandra Fleischmann
I am working in the field of mitochondrial biochemistry since many years. In detail my coworkers and I are carrying out studies on the regulation of ATP synthase and respiratory complexes in swine heart mitochondria as biological model of mammalian mitochondria. Some comparative aspects of mitochondrial bioenergetics and ATP synthase responses are also investigated in bivalve mollusk mitochondria. - Alessandra Pagliarani (2016).
We are mostly interested in two WG: (1) Since we are working mostly with heart and muscles, we are interested in WG 2 - MITOEAGLE data repository in muscle and other tissues. (2) WG 1 - Standard operating procedures and user requirement document: Protocols, terminology, documentation. Since we have an expertise in fatty acid metabolism, we are specially interested in experimental protocols for the evaluation of mitochondrial capacities regarding fatty acid metabolism. Of course our parts of the WG are also in our interest. - Marina Makrecka-Kuka, Edgars Liepinsh (2016).
My role in Mito-EAGLE would be mainly in WG 2 and 4, with a focus on skeletal and cardiac muscle and cultured cardiomyocytes and endothelial cells. I would particularly like to bridge the gap between the group leaders and young investigators, trying to represent the young people who are the active users of the OROBOROS equipment. This will fit in nicely with the goals in WG1. ... Major congratulations on obtaining this beautiful collaborative grant! Very glad to see this network getting the recognition it deserves! Looking forward to being part of this! - Rob C Wuest (2016).
We mostly work with cultured human skeletal muscle cells, from different donor groups such as lean, obese, type 2 diabetics, as well as muscle cells from athletes. We are interested in collaboration with our cells and measurements on energy metabolism and mitochondral function. - Arild Rustan (2016).
Please, find enclosed my declaration to participate with my team in the following workgroups: WG1, WG2, WG3, WG4. We are now involved and have been in not a distant past – in studying of the following cell /tissue types:
1. Blood cells, with particular emphasis on platelets and lymphocytes – isolated from animals and humans.
2. Endothelial cells, fibroblasts, neurocytes (neuroblastoma), astrocytes, cancer cell lines – predominantly cultured.
3. Hepatocytes, cardiomyocytes, cell isolated from brain – isolated from lab animals. - Cezary Watala (2016).
I have already contacted the portuguese CNC to nominate me for the MC of theaction. Concerning my active participation, taking into account the type of work we do in my lab I could be included in all WGs. As you know we work with mitochondria from liver, muscle and fat, as well as cultured cells from different origins (WG2-4). The elaboration of SOPs (WG1) we can certainly contribute (see the book I edited on Mitochondrial Bioenergetics and in which you contribute a chapter). I believe that everybody can be part of the WG5, but as the coordinators of the action, you and Sandra should decide what is best for the success of the action. - Carlos Palmeira (2016).
As you know, I have received the MC nomination from our CNC in Bucharest (Ms Elena Dinu) for my colleague, Rodica Lighezan and myself. My expertise is most on muscle tissue (in particular, heart) and also, liver - so I will go for WG2 if this is fine with you. However, Both my colleague and I are mostly interested in performing respirometry in blood cells (and this is what we will start to try in the near future); thus, you decide whether we can fit both of us in WG4, or only her. Also, in order to extend the Network in other Romanian cities with powerful state medical universities, Cluj-Napoca (as you know) and Tg Mures - 2 young men, Bela Kiss and Ovidiu Cotoi have been proposed as substitute members (for gender balance too). In particular, we want to jointly ”explore” the exciting fields of mitochondrial ”hidden toxicity” and dynamics, respectively since the former colleague is a toxicologist and the latter a pathologist. I wish you good luck and I am indeed happy and proud to be part of this growing network. - Danina M Muntean (2016).
I took a look to the MoU and the different working groups. You know well my research focus and having this into account I am interested in actions related to Working group 1 (Respirometric reference protocols, Interlaboratory proficiency test, Instrumental platforms). Working group 2 and 3: I have generated multiple mitochondrial respiration data sets in different mouse tissues (mainly in skeletal muscle, hypothalamus, liver and white adipose tissue). Currently we are also performing these assays in white adipose tissue from human patients. Hence I am interested in all milestones of WG 2 and 3. WG 5 is related to WG 1 and as you know I have interest in training, dissemination and also together with my collaborators (if needed) we could help with data sets and a data interpretation. Overall, I have a general interest on this Action and my active role in MITOEAGLE could be discussed. I could be of help participating in some of the tasks where my expertise and interest fit best. We will be forming and interdisciplinary research group with four group leaders: JC Perales, R Guimera, M Sales-Pardo and PM Garcia-Roves with two different affiliations, University of Barcelona (JCP and PMG-R - Energy metabolism) and University Rovira I Virgili (RG and MS – Data base, statistics, data mining). - Pablo M Garcia-Roves (2016).
Congratulations on the considerable progress with MITOEAGLE! I am keen to participate in this exciting project and I’m sure I can make a valuable contribution to it's success. I have extensive experience performing respirometry assays in human muscle biopsies, myoblasts/tubes and mouse tissues in the contexts of aging, obesity, exercise and inactivity. I feel that I could contribute to any of the five working groups, but particularly to WG2, WG4, and WG5. I am also happy and willing to serve on the Management Committee if needed. Again, congratulations on organizing such an exciting project. - Paul M Coen (2016).
In particular, my team can participate with studies about the mitochondrial function in relation to the supramolecular organization of the respiratory chain where the dynamic presence of the respiratory supercomplexes is a way to shift between the main metabolic fluxes of NAD- and FAD-dependent substrates in different metabolic conditions of the cell. Besides my basic studies on the enzyme interactions with the Coenzyme Q pool, I recently joined an Italian consortium of University laboratories whose target is to shed light on the molecular mechanisms underpinning muscle weakness and reduced muscle mass (sarcopenia) in ageing and attenuated response to exercise training stimuli in the elderly; both animal models and human subjects will be analyzed. - Maria Luisa Genova (2016).
I would be interested to be involved with WG1 and WG2. In particular, I am interested in contributing to developing protocols for multicenter quality control among labs, and for permeabilized muscle bundle respirometry. - Harry B Rossiter (2016).
Our main research subject is the heart muscle cells. So, we could contribute in datasets for several animals (rat, trout, mice [WT and KOs]). In collaboration with WG1, it would be possible to report data in standardized format. - Marko Vendelin (2016)
As research group working in the field of skeletal muscle research (development,function and regeneration, metabolic pathways, signalling, and energy metabolism and skeletal muscle in special condition-ischemia, hypoxia, sepsis) we found several topics in the COST Action particularly interesting. Indeed, we are highly interested in effects of media and protocols on experimental readouts in cell cultures (please see Pirkmajer 2011 Am J Physiol Cell Physiol and Rajh 2016 PLOS ONE). We therefore find the focus on harmonization and validation of protocols and terminology especially important. - Tomaz Mars (2016).

Projects in progress

» MiPMap

Next steps

» MITOEAGLE data repository in muscle tissues