|News and Events||Working Groups||Short-Term Scientific Missions||Management Committee||Members|
COST Action CA15203 (2016-2021): MitoEAGLE
Evolution-Age-Gender-Lifestyle-Environment: mitochondrial fitness mapping
MitoPedia topics: EAGLE
|Institution||University of Nottingham, UK|
|Address||Sutton Bonnington Campus, LE12 5RD|
|O2k-Network Lab||UK Nottingham Chakrabarti L|
MitoEAGLE Short-Term Scientific Mission
- Work Plan summary
- There are three primary research goals in my plan for the STSM that I propose. The first of these is to extract live tissues from the polar cod B. saida, and then to perform various mitochondrial assays on these tissues. As my academic background is based in biochemistry and chemistry, and my research to date has been built around the biochemistry and protein biology of mitochondria this would be an excellent opportunity to train in the use of live tissue. This training would enable my research to become more interdisciplinary in its nature and would provide me with greater flexibility in the ways I can respond to experimental results that I produce.
- Work Plan summary
- A particular mitochondrial assay, and the second of the research goals that I seek to pursue, would include measuring the respirometric profile of the live tissue through the use of an Oroboros O2k Respirometer. As my research at the University of Nottingham is in part structured around the mitochondrial biology of a different family of cold water fish, Notothenioidei, this training would allow me to benefit from the expertise of Dr Mark at the AWI in conducting cold temperature mitochondrial research. Upon returning to Nottingham I would be better equipped to tackle the questions that form the basis of the PhD project, and would be able to apply what I had learnt about using the O2k at cold temperatures to the O2k our lab possesses in Nottingham.
- The third aim would be to undertake molecular assays that will contribute to our understanding of the role of haemoglobin within the mitochondria. In particular, to explore the physical interaction between mitochondrial haemoglobin and ATP synthase. Undertaking this work with the polar cod B. saida will allow the development of protocols to be repeated in the Notothenioidei family, whose members do not express myoglobin, and in some cases do not express haemoglobin either.
- From the work that has been outlined above, should it prove to be successful, this will be able to form the basis of a manuscript that will be jointly published between the collaborators on the STSM grant at the University of Nottingham and those at the Alfred Wegener Institute.
- I believe that the STSM would be a wonderful opportunity as an early career researcher. As is outlined above it will allow me to develop new wet-lab skills that will broaden the horizons of my research aims, which in turn will allow me to conduct more impactful research. To experience research in another European country, in an unfamiliar field, would accelerate my development as a young scientist who is equipped to work in the cross-disciplinary, international scientific community. As a second year PhD student this STSM would take place in the very early stages of my scientific career. As such I would stand to gain a better understanding of how international collaborations can be established, and how pooling expertise from different fields can take place in practise.
- The STSM that I am proposing will also serve to further reinforce collaboration between our group at the University of Nottingham and the scientists at the AWI in Bremerhaven. Their expertise in the anatomy, physiology and ecology of polar and marine species will provide me with new outlooks and better understanding of both my current work and future research that I hope my career will lead to. To continually reinforce these relationships is crucial to pan-European research and may provide the foundation to larger and more ambitious collaborations, which I hope to participate in throughout my research career.
- Through participation in the STSM, I hope to further the strategic aims of both the mitoEAGLE and COST communities. A stronger understanding of mitochondrial biology will serve to benefit our understanding of human health and disease, however the overwhelming volume of publications and research into mitochondrial biology has the capacity to be counterproductive in this ambition. The participation in the mitoEAGLE network by Dr Chakrabarti and Dr Mark is part of a unique drive to systemize the research in this domain. It is through the standardisation of mitochondrial research across disciplines that will enable the community to conduct both impactful and novel research that will improve outcomes in health and disease.
|Krako Jakovljevic 2021 BEC PD||2021||Krako Jakovljevic N, Ebanks B, Katyal G, Chakrabarti L, Markovic I, Moisoi N (2021) Mitochondrial homeostasis in cellular models of Parkinson’s Disease. https://doi.org/10.26124/bec:2021-0002|
|Ebanks 2022 Abstract Bioblast||2022||P09. https://doi.org/10.26124/bec:2022-0001|
|Chakrabarti L 2022 Abstract Bioblast||2022||1.2. «10+5» https://doi.org/10.26124/bec:2022-0001|