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COST Action CA15203 (2016-2021): MitoEAGLE
Evolution-Age-Gender-Lifestyle-Environment: mitochondrial fitness mapping
MitoEAGLE data: blood and cultured cells
WG4
- MITOEAGLE data repository for blood cells and clutured cells
WG4 Description
- Biopsy sampling of human muscle and fat tissue for mitochondrial studies is an invasive approach. More recently measurement of mitochondrial function in human blood cells – as well as temporary cryopreservation (Karabatsiakis 2014 Transl Psychiatry) – has become an attractive and by far less invasive alternative for mitochondrial diagnostics. Mitochondrial function of blood cells was linked with numerous diseases and metabolic conditions such as sepsis, neurodegenerative diseases, depression, hypertension, amyotrophic lateral sclerosis, and early-stage heart failure (e.g. Sjoevall 2013 Crit Care; Karabatsiakis 2014 Transl Psychiatry; Hagl 2014 J Alzheimer Dis). The use of human blood cells will thus predictably widen the applicability of respirometry in human physiology and will provide a significant stimulus for the entire field of mitochondrial medicine.
- A key towards achieving comparability among data sets and the applicability of human blood cells for the evaluation of mitochondrial fitness in health and disease is the standardization of the procedures to separate or isolate blood cells, the experimental procedure for the evaluation of mitochondrial respiratory characteristics, and the format for reporting. A consensus on protocols should be reached in WG4, calling for a Workshop right at the start of the COST Action. Depending on the WG4 participants input, cell lines will be defined for which harmonization of datasets should be achieved (Fig. 2).
WG4 Participants
| Country | City | Contact |
|---|---|---|
| Armenia | Yerevan | Trchounian K |
| Austria | Innsbruck | Gnaiger Erich |
| Austria | Innsbruck | Cardoso Luiza HD |
| Austria | Innsbruck | Garcia-Souza Luiz F |
| Austria | Innsbruck | Doerrier Carolina |
| Austria | Innsbruck | Fischer Christine |
| Austria | Innsbruck | Karabatsiakis Alexander |
| Austria | Innsbruck | Meszaros Andras T |
| Austria | Innsbruck | Torres-Quesada Omar |
| Austria | Innsbruck | Hoppel Florian |
| Belgium | Namur | Arnould Thierry |
| Belgium | Liege | Votion Dominique-Marie |
| Belgium | Namur | Detraux Damien |
| Belgium | Namur | Renard P |
| Belgium | Leuven | Bird Matthew |
| Bosnia and Herzegovina | Tuzla | Jusic Amela |
| Bosnia and Herzegovina | Tuzla | Hajrulahovic Anesa |
| Brazil | São Paulo | Crispim Marcell |
| Brazil | Sao Paulo | Verdaguer Ignasi Bofill |
| Brazil | Sao Paulo | Dominguez-Amorocho OA |
| Brazil | Sao Paulo | Alencar Mayke Bezerra |
| Croatia | Zagreb | Rubelj Ivica |
| Czech Republic | Prague | Mracek Tomas |
| Czech Republic | Pilsen | Kripnerova M |
| Czech Republic | Vestec | Zobalova Renata |
| Czech Republic | Prague | Elkalaf Moustafa |
| Czech Republic | Hradec Kralove | Vernerova Andrea |
| Czech Republic | Pilsen | Markova Michaela |
| Czech Republic | Hradec Kralove | Maseko Tumisang Edward |
| Czech Republic | Prague | Fisar Zdenek |
| Czech Republic | Pilsen | Kuncova J |
| Czech Republic | Prague | Pecina Petr |
| Czech Republic | Prague | Hansikova Hana |
| Czech Republic | Prague 4 | Bajzikova Martina |
| Czech Republic and Australia | Prague and Southport | Neuzil Jiri |
| Czech rep | Hradec Kralove | Falaye T |
| Denmark | Hvidovre | Nehlin Jan O |
| Denmark | Aarhus | Rattan Suresh IS |
| Denmark | Malov | Hey-Mogensen M |
| Denmark | Copenhagen | Desler C |
| Denmark | Copenhagen | Rasmussen LJ |
| Denmark | Copenhagen N | Mortensen OH |
| Egypt | Cairo | Ali Sameh S |
| Egypt | Cairo | Abdel-Rahman Engy Ali |
| Estonia | Tallinn | Klepinina Lyudmila |
| Estonia | Tallinn | Truu Laura |
| Estonia | Tartu | Grellier T |
| Finland | Turku | Stier Antoine |
| Finland | Jyvaeskylae | Lehti Maarit |
| Finland | Helsinki | Pirinen E |
| France | Paris | Petit Patrice X |
| France | Bordeaux cedex | Mourier A |
| France | Bron | Rizwan Alam M |
| Germany | Leipzig | Ost Mario |
| Germany | Duesseldorf | Piel Sarah |
| Germany | Regensburg | Renner-Sattler Kathrin |
| Germany | Mainz | Kalinovic S |
| Germany | Magdeburg | Vieira Ligo Teixeira Camila |
| Germany | Freising | Keppner Gloria |
| Germany | Giessen | Eckert Gunter P |
| Germany | Freising-Weihenstephan | Musiol Eva |
| Germany | Munich | Jastroch M |
| Germany | Hamburg | Singer Dominique |
| Germany | Ulm | Hartmann C |
| Germany | Biberach an der Riß | Nold V |
| Germany | Freising | Schoettl T |
| Germany | Duesseldorf | Eckers A |
| Germany | Ulm | Calzia Enrico |
| Germany | Neuherberg | Borchard Sabine |
| Germany | Ulm | Schaefer PM |
| Germany | Ulm | Kolassa Iris |
| Germany | Gießen | Silaidos Carmina |
| Germany | Düsseldorf | Prigione Alessandro |
| Germany | Freising | Fromme Tobias |
| Germany | Mainz | Vujacic-Mirski Ksenija |
| Germany | Freising | Klingenspor Martin |
| Greece | Athens | Trougakos Ioannis P |
| Hungary | Budapest | Tretter Laszlo |
| Hungary | Budapest | Komlodi Timea |
| Hungary | Debrecen | Verebne Tar K |
| Ireland | Dublin | Porter Richard K |
| Ireland | Sligo | Murphy J |
| Ireland | Dublin | Karavyraki Marilena |
| Israel | Rishon Le Zion | Hachmo Yafit |
| Israel | Jerusalem | Saada Reisch Ann |
| Israel | Zrifin | Sova Marina |
| Italy | Bolzano/Bozen | Volani Chiara |
| Italy | Bologna | Paterlini S |
| Italy | Padova | Fernandez-Vizarra Erika |
| Italy | Bologna | Porcelli AM |
| Italy | Bolzano | Castelo Rueda Maria Paulina |
| Italy | Ancona | Battino Maurizio |
| Italy | Bologna | Gasparre G |
| Italy | Rome | Scatena Roberto |
| Italy | Milano | Bottoni P |
| Italy | Verona | Calabria Elisa |
| Italy | Bologna | Genova Maria Luisa |
| Italy | Bolzano | Pichler Irene |
| Japan | Sapporo | Shirakawa R |
| Latvia | Riga | Kidere Dita |
| Latvia | Riga | Makarova Elina |
| Malaysia | Kuala Lumpur | Abdul Karim Norwahidah |
| Malta | Msida | Vella Joanna |
| Netherlands | Groningen | Rots MG |
| Netherlands | Wageningen | Keijer Jaap |
| Netherlands | Nijmegen | Koopman Werner JH |
| Norway | Oslo | Nikolic N |
| Norway | Oslo | Rustan AC |
| Norway | Bergen | Tronstad Karl Johan |
| Norway | Oslo | Lund Jenny |
| Norway | Oslo | Eide L |
| Palestine | Ramallah | Stiban Johnny |
| Poland | Poznan | Swiniuch Daria |
| Poland | Lodz | Siewiera Karolina |
| Poland | Warsaw | Wasilewski M |
| Poland | Krakow | Kaczara P |
| Poland | Poznan | Michalak Slawomir |
| Poland | Warsaw | Kampa Rafal Pawel |
| Poland | Warsaw | Sek Aleksandra |
| Poland | Poznan | Rybacka-Mossakowska Joanna |
| Poland | Warsaw | Dymkowska Dorota |
| Poland | Lodz | Watala Cezary |
| Poland | Poznań | Ludziejewska A |
| Poland | Lodz | Labieniec-Watala Magdalena |
| Portugal | Porto | Moreira Bruno P |
| Portugal | Cantanhede | Bento Guida Jose Freitas |
| Portugal | Porto | Oliveira Pedro Fontes |
| Portugal | Covilhã | Dias Tania R |
| Portugal | Coimbra | Palmeira Carlos |
| Portugal | Porto | Alves Marco G |
| Portugal | Coimbra | Pereira Susana P |
| Portugal | Porto | Bernardino RL |
| Portugal | Cantanhede | Pinho Sonia A |
| Portugal | Catanhede | Pereira da Silva Grilo da Silva Filomena |
| Portugal | Porto | Beleza Jorge |
| Portugal | Porto | Carrageta D |
| Portugal | Porto | Magalhaes Jose |
| Portugal | Porto | Martins Ana Dias |
| Portugal | Porto | Crisostomo Luis |
| Portugal | Coimbra | Carvalho Eugenia |
| Republic of Korea | Daejeon | Park SH |
| Republic of Korea | Yuseong-gu Daejeon | Quang Don T |
| Republic of Serbia | Belgrade | Pavlovic Kasja |
| Republic of Serbia | Belgrade | Krako Jakovljevic Nina |
| Romania | Timisoara | Avram Vlad F |
| Romania | Timisoara | Danila Maria-Daniela |
| Romania | Timisoara | Muntean Danina M |
| Romania | Timisoara | Lelcu Theia |
| Romania | Timisoara | Lighezan R |
| Romania | Timisoara | Betiu AM |
| Russia | Moscow | Itkis YS |
| Russia | Moscow | Tsygankova P |
| Russia | Moscow | Krylova T |
| Saudi Arabia | Giza | Kotb IA |
| Serbia | Belgrad | Markovic Ivanka |
| Serbia | Belgrade | Misirlic-Dencic S |
| Serbia | Belgrade | Isakovic A |
| Serbia | Belgrade | Ivanovic Tijana |
| Serbia | Belgrade | Jeremic M |
| Serbia | Belgrade | Vukojevic M |
| Slovakia | Bratislava | Ukropcova B |
| Slovakia | Bratislava | Maderova D |
| Slovakia | Bratislava | Ukropec J |
| Slovakia | Bratislava | Nemec Michal |
| Slovakia | Bratislava | Sumbalova Zuzana |
| Slovakia | Košice | Cizmarova Beata |
| Slovenia | Ljubljana | Kopitar-Jerala Natasa |
| Slovenia | Ljubljana | Pirkmajer Sergej |
| Slovenia | Ljubljana | Mars T |
| Spain | Madrid | Cano-Abad MF |
| Spain | Lleida | Gonzalo Hugo |
| Spain | A Coruña | Blanco FJ |
| Spain | Madrid | Monsalve Maria |
| Spain | Valencia | Borras Consuelo |
| Spain | Madrid | Lopez MG |
| Spain | Lleida | Nogueras L |
| Spain | Barcelona | Dahdah Norma Ramon |
| Spain | Madrid | Cadenas S |
| Spain | Valencia | Fuertes Agudo Marina |
| Spain | Sevilla | Muntane Jordi |
| Spain | Barcelona | Gonzalez Casacuberta I |
| Spain | Sevilla | Bustos Matilde |
| Spain | Salamanca | Almeida Angeles |
| Spain | Barcelona | Tura Ceide O |
| Spain | Valencia | Casado Pinna Marta |
| Spain | Granada | Quiles JL |
| Spain | Barcelona | Arago Marc |
| Spain | Barcelona | Hyrossova Petra |
| Spain | Barcelona | Juarez D |
| Spain | Barcelona | Zorzano Antonio |
| Spain | A Coruña | Mayan MD |
| Spain | Armilla (Granada) | Fernandez-Ortiz Marisol |
| Spain | Barcelona | Smolders V |
| Spain | Madrid | De los Rios C |
| Spain | Barcelona | Gil J |
| Spain | Valencia | Victor Victor Manuel |
| Spain | Barcelona | Gama Perez Pau |
| Spain | Armilla, Granada | Escames Germaine |
| Spain | Ourense | Reboredo-Rodriguez Patricia |
| Sweden | Lund | Simon Serrano Sonia |
| Sweden | Lund | Leffler M |
| Sweden | Lund | Abliva |
| Sweden | Lund | Westerlund E |
| Sweden | Lund | Karlsson M |
| Sweden | Linköping | Fontes-Oliveira CC |
| Sweden | Lund | Elmer Eskil |
| Sweden | Lund | Chamkha Imen |
| Sweden | Södertälje | Alfaro-Moreno E |
| Sweden, SE | Lund | Aasander Frostner Eleonor |
| Switzerland | Lausanne | Zanou Nadege |
| Switzerland | Geneva | Martinou JC |
| The Netherlands | BT Amsterdam | Wuest Rob CI |
| Turkey | Ankara | Tuncay Erkan |
| Turkey | Ankara | Engin Ayse Basak |
| Turkey | Istanbul | Baykal AT |
| Turkey | Tekirdag | Aral Cenk |
| Turkey | Izmir | Aydin HH |
| USA | St Louis | Welch R |
| USA | Fayetteville | Rao RR |
| USA | Philadelphia | Starr Jonathan |
| USA | Philadelphia | Kilbaugh Todd |
| USA | Baton Rouge | Irving Brian A |
| USA | Orlando | Coen Paul M |
| USA | Philadelphia | Janowska Joanna |
| USA | Fayetteville | Iyer Shilpa |
| USA | Iowa City | Buettner Garry R |
| United Kingdom | Oxford | Makrecka-Kuka Marina |
| United Kingdom | Leicester | Moisoi Nicoleta |
| United Kingdom | Birmingham | Thakker A |
| United Kingdom | Birmingham | Joanisse S |
| United Kingdom | Brighton | Moore Anthony L |
| United Kingdom | Stoke-on-Trent | McStay Gavin P |
| Uruguay | Montevideo | Radi R |
WG4 Management
Tasks
- Kick-off meeting to develop a working plan for the establishment of consensus protocols, reporting schemes, and the assignment of specific study tasks.
- Development of SOPs for cell separation.
- Development of laboratory protocols for individual blood cell types (and other cell types) for mitochondrial studies on intact and permeabilized cells.
- Application of SOPs in experimental studies during Short Term Scientific Missions and feeding of data into the MITOEAGLE data repository.
- Publication of SOPs and study results.
Milestones
- Consensus on protocols, reporting schemes and work assignments.
- Completed SOPs for cell preparation & laboratory protocols.
- Application study finished and data transmitted to MITOEAGLE data repository.
- Publication finished.
Deliverables
- SOPs for blood cell separation and respirometric characterisation open for the research community.
- MITOEAGLE data repository for comparative data evaluation, planning of future studies, data mining.
- Publication with a set of reference data.
