Meszaros Andras T

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COST Action CA15203 (2016-2021): MitoEAGLE
Evolution-Age-Gender-Lifestyle-Environment: mitochondrial fitness mapping

Meszaros Andras T

MitoPedia topics: EAGLE

COST: Member COST WG1: WG1

COST WG4: WG4

COST ECI: ECI

Name	Mészáros András T, MD, PhD
Institution	Mitochondrial Medicine Visionary Medical University of Innsbruck Departement of Visceral-Transplant and Thoracic Surgery Research on: Biologically active gases, nitric oxide, mitochondrial membrane potential, oxygen kinetics Andras collaborates with Oroboros Instruments since April 2017.
Address	Anichstraße 35, 6020
City	Innsbruck
State/Province
Country	Austria
Email	[email protected]
Weblink	https://www.researchgate.net/profile/Andras_Meszaros2 https://www.chirurgie-innsbruck.at/en/team/d/87/dr-andras-meszaros
O2k-Network Lab	AT Innsbruck Oroboros, AT Innsbruck Schneeberger S, HU Szeged Boros M

Labels: Field of research: Basic Topics: hypoxia, reoxygenation, electrophyl methyl groups (EMGs), methane, long-chain fatty acids

Publications

	Published	Reference
Meszaros 2024 Transpl Int	2024	Meszaros AT, Weissenbacher A, Schartner M, Egelseer-Bruendl T, Hermann M, Unterweger J, Mittelberger C, Reyer BA, Hofmann J, Zelger BG, Hautz T, Resch T, Margreiter C, Maglione M, Komlódi T, Ulmer H, Cardini B, Troppmair J, Öfner D, Gnaiger E, Schneeberger S, Oberhuber R (2024) The predictive value of graft viability and bioenergetics testing towards the outcome in liver transplantation. Transpl Int 37:12380. https://doi.org/10.3389/ti.2024.12380
Meszaros 2022 EBioMedicine	2022	Meszaros AT, Hofmann J, Buch ML, Cardini B, Dunzendorfer-Matt T, Nardin F, Blumer MJ, Fodor M, Hermann M, Zelger B, Otarashvili G, Schartner M, Weissenbacher A, Oberhuber R, Resch T, Troppmair J, Öfner D, Zoller H, Tilg H, Gnaiger E, Hautz T, Schneeberger S (2022) Mitochondrial respiration during normothermic liver machine perfusion predicts clinical outcome. https://doi.org/10.1016/j.ebiom.2022.104311
Bastos Sant'Anna Silva 2021 Cancers (Basel)	2021	Bastos Sant'Anna Silva AC, Perez Valencia JA, Sciacovelli M, Lalou C, Sarlak S, Tronci L, Nikitopoulou E, Meszaros AT, Frezza C, Rossignol R, Gnaiger E, Klocker H (2021) Succinate anaplerosis has an onco-driving potential in prostate cancer cells. https://doi.org/10.3390/cancers13071727
BEC 2020.1 doi10.26124bec2020-0001.v1	2020	Gnaiger E et al ― MitoEAGLE Task Group (2020) Mitochondrial physiology. Bioenerg Commun 2020.1. https://doi.org/10.26124/bec:2020-0001.v1
Di Marcello 2019 MitoFit Preprint Arch EA	2019	Di Marcello M, Iglesias-Gonzalez J, Meszaros A, Haider M, Gnaiger E, Huete-Ortega M (2019) Partitioning between cytochrome c oxidase and alternative oxidase studied by oxygen kinetics of dark respiration in Chlamydomonas reinhardtii: a microalgae model organism. https://doi.org/10.26124/mitofit:ea19.MiPSchool.0005.v3
Rutai 2019 Shock	2019	Rutai A, Fejes R, Juhász L, Tallósy SP, Poles MZ, Földesi I, Mészáros AT, Szabó A, Boros M, Kaszaki J (2019) Endothelin A and B receptors: Potential pargets for microcirculatory-mitochondrial therapy in experimental sepsis. Shock 54:87-95.
Doerrier 2018 Methods Mol Biol	2018	Doerrier C, Garcia-Souza LF, Krumschnabel G, Wohlfarter Y, Mészáros AT, Gnaiger E (2018) High-Resolution FluoRespirometry and OXPHOS protocols for human cells, permeabilized fibers from small biopsies of muscle, and isolated mitochondria. Methods Mol Biol 1782:31-70. https://doi.org/10.1007/978-1-4939-7831-1_3
Rademann 2017 Oxid Med Cell Longev	2017	Rademann P, Weidinger A, Drechsler S, Meszaros A, Zipperle J, Jafarmadar M, Dumitrescu S, Hacobian A, Ungelenk L, Röstel F, Kaszaki J, Szabo A, Skulachev VP, Bauer M, Bahrami S, Weis S, Kozlov AV, Osuchowski MF (2017) Mitochondria-targeted antioxidants SKQ1 and MitoTEMPO failed to exert a long-term beneficial effect in murine polymicrobial sepsis. Oxid Med Cell Longev 6412682.
Tuboly 2017 Sci Rep	2017	Tuboly E, Molnár R, Tőkés T, Turányi RN, Hartmann P, Mészáros AT, Strifler G, Földesi I, Siska A, Szabó A, Mohácsi Á, Szabó G, Boros M (2017) Excessive alcohol consumption induces methane production in humans and rats. Sci Rep 7:7329.
Strifler 2016 PLOS ONE	2016	Strifler G, Tuboly E, Szél E, Kaszonyi E, Cao C, Kaszaki J, Mészáros A, Boros M, Hartmann P (2016) Inhaled methane limits the mitochondrial electron transport chain dysfunction during experimental liver ischemia-reperfusion injury. PLOS ONE 11:e0146363.
Strifler 2016 Magyar Sebeszet	2016	Strifler G, Meszaros A, Pecz D, Ficzere A, Barath B, Mihaly B, Hartmann P (2016) A májfunkció vizsgálata respirometriával - Examination of liver mitochondria with respirometry. Magyar Sebészet 69:194–8.

Abstracts

	Published	Reference
Meszaros 2022 Abstract Bioblast	2022	6.6. «5 min» Meszaros Andras T, Hofmann J, Gnaiger E, Oberhuber R, Hautz T, Öfner D, Schneeberger S (2022) Pre-transplant mitochondrial respiration as a clinical prognostic marker during static cold storage and machine perfusion of the liver. Bioblast 2022: BEC Inaugural Conference. In: https://doi.org/10.26124/bec:2022-0001
Bastos Sant'Anna Silva 2019 Life Sciences Meeting 2019 Innsbruck AT	2019	Cellular succinate transport and mitochondrial respiratory function in prostate cancer.
Rutai 2019 Crit Care	2019	Rutai A, Fejes R, Tallósy SZ, Poles M, Juhász L, Mészáros A, Boros M, Kaszaki J (2019) Potential role of endothelin receptors in the therapy of experimental sepsis. Crit Care.
Meszaros 2018 Mt Med Hinxton	2018	High-resolution mitochondrial oxygen kinetics as diagnostic tool in Complex IV impairments.
Meszaros 2018 EBEC2018	2018	Tissue- and substrate-specific patterns in the oxygen kinetics of mitochondrial respiration.
Bastos Sant'Anna Silva 2018 TRANSMIT Bertinoro di Romagna IT	2018	Cellular succinate transport and mitochondrial respiratory function in prostate cancer.
Bastos Sant'Anna Silva 2018 MiPschool Tromso C4	2018	Role of succinate in prostate cancer cells: uptake and mitochondrial respiratory function.
Bastos Sant'Anna Silva 2018 Life Sciences Meeting 2018 Innsbruck AT	2018	Effect of cell-permeable succinate and malonate prodrugs on mitochondrial respiration in prostate cancer cells.
Meszaros 2017 MiPschool Obergurgl	2017	(Very) High-resolution respirometry – a deep dive into oxygen dependence of mitochondrial respiration.
Meszaros 2017 MiP2017	2017	Mitochondrial oxygen kinetics as a sensitive diagnostic tool to determine apparent cytochrome c oxidase excess capacity.
Meszaros 2014 Abstract MiP2014	2014	Methodological aspects of assessing mitochondrial function in small intestinal mucosal samples of rats and guinea pigs.

MitoEAGLE Short-Term Scientific Mission

- - - STSM Grant Period 1

Motivation letter

My name is András Mészáros and I am working as associate professor in the Institute of Surgical Research at the University of Szeged, Hungary. I got my M.D. degree in 2012 and I was working as PhD fellow in the same institute for three years subsequently before taking the current position. My main research interest is to understand mitochondrial dysfunction during oxido-reductive stress conditions (hypoxia, ischemia-reperfusion) in animal models and cultured cells. My further aim is to characterize acting mechanism of novel biologically active gaseous compounds in mitochondria. I have been working with High Resolution Respirometry and basic fluorometry for three years, gaining experience maínly with tissue homogenate and isolated mitochondría. An essential step now is to extend my expertise toward assessment of other functions than oxygen consumption.

The main objective of the planned STSM training is to get acquainted with and to standardize fluorometric measurements of isolated mitochondria and cultured cells. During the 8 days of short-term visit at the MitoFit Laboratory three new techniques could be learned:(l) measurement of reactive oxygen species production of permeabilized cells using H2O2-sensitive fluorescent probes; (2) determination of mitochondrial membrane potential and (3) assessment of mitochondrial phosphate/oxygen ratio by measuring ATP levels fluorometrically. Preparation and execution of each method will be learned with emphasis on internal quality control and possible adaptation of measurement protocols to samples from various tissues. Furthermore, we are going to use tools of complex data analysis and calibration.

Fulfillment of the proposed research plan during the STSM training would contribute significantly to the objectives of the MitoEAGLE Working Group 1 (standardization of protocols in respirometry).I wish to actively participate in the MitoEAGLE project, in, among others, establishing and testing reference protocols to provide basis for comparison of results obtained in various laboratories. To this end, a short stay at the MitoFit Laboratory of Oroboros Instruments could provide a perfect platform having up-to-date instrumental background, well established measurement protocols and top level proficiency.

STSM request: download pdf

MitoEAGLE

- - - Benefits for COST and for the COST Action MitoEAGLE

I am working mainly with immune cells from blood and liver mitochondria, while enterocytes and epithelial cells are the major targets of my plans for the next few months.

Participated at

Visiting scientist in the Oroboros O2k-Laboratory

András Mészáros : Visiting scientist at the Oroboros O2k-Laboratory

2016, November 21 to 28