Template:SUIT-003
Steps and respiratory states
Step | State | Pathway | Q-junction | Comment - Events (E) and Marks (M)
ROUTINE respiration in the physiological coupling state R. Externally added permeable substrates could contribute to this respiratory state. |
---|---|---|---|---|
ce2Omy | ROX | CII | ce1;ce2Rot
Succinate pathway control state (S-pathway) after inhibiting CI with rotenone, which also inhibits the F-pathway. | |
ce3S | SL | S | CII | ce1;ce2Rot;ce3S
Succinate, S ( type S-pathway to Q). Template:SUIT L n |
ce4D | SP | S | CII | ce1;ce2Rot;ce3S;ce4D
Succinate, S ( type S-pathway to Q). OXPHOS capacity P (with saturating [ADP]), active OXPHOS state. |
1Dig | SP | S | CII | ce1;ce2Rot;ce3S;ce4D;1Dig
Succinate, S ( type S-pathway to Q). Multiple Digitonin titrations for complete plasma membrane permeabilization. OXPHOS capacity P (with saturating [ADP]), active OXPHOS state. |
1c | SP | S | CII | ce1;ce2Rot;ce3S;ce4D;1Dig;1c
Succinate, S ( type S-pathway to Q). Addition of cytochrome c yields a test for integrity of the mtOM (cytochrome c control efficiency). Stimulation by added cytochrome c would indicate an injury of the mtOM and limitation of respiration in the preceding state without added c due to loss of cytochrome c. Typically, cytochrome c is added immediately after the earliest ADP-activation step (OXPHOS capacity P with saturating [ADP]). OXPHOS capacity P (with saturating [ADP]), active OXPHOS state. |
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- Coupling control
- Pathway control
- Β» Electron transfer pathway
- Β» Fatty acid oxidation pathway control state, F
- Β» NADH electron transfer-pathway state, N
- Β» Succinate pathway control state, S
- Β» NS-pathway control state, NS
- Β» Glycerophosphate pathway control state, Gp
- Β» Complex IV single step, CIV
- Β» Anaplerotic pathway control state
- Pathway control
- Main fuel substrates
- Β» Glutamate, G
- Β» Glycerophosphate, Gp
- Β» Malate, M
- Β» Octanoylcarnitine, Oct
- Β» Pyruvate, P
- Β» Succinate, S
- Main fuel substrates
- Glossary