Search results

...ategory:Publications]] [[Instrument and method::Oxygraph-2k]] [[Mammal and model::Other mammals]] |?Mammal and model=Organism

...ategory:Publications]] [[Instrument and method::Oxygraph-2k]] [[Mammal and model::Plants]] |?Mammal and model=Organism

...ategory:Publications]] [[Instrument and method::Oxygraph-2k]] [[Mammal and model::Human]] |?Mammal and model=Organism

...ategory:Publications]] [[Instrument and method::Oxygraph-2k]] [[Mammal and model::Drosophila]] |?Mammal and model=Organism

...ategory:Publications]] [[Instrument and method::Oxygraph-2k]] [[Mammal and model::Eubacteria]] |?Mammal and model=Organism

...ategory:Publications]] [[Instrument and method::Oxygraph-2k]] [[Mammal and model::Archea]] |?Mammal and model=Organism

...ategory:Publications]] [[Instrument and method::Oxygraph-2k]] [[Mammal and model::Protists]] |?Mammal and model=Organism

...ategory:Publications]] [[Instrument and method::Oxygraph-2k]] [[Mammal and model::Hexapods]] |?Mammal and model=Organism

...ategory:Publications]] [[Instrument and method::Oxygraph-2k]] [[Mammal and model::Fungi]] |?Mammal and model=Organism

...ategory:Publications]] [[Instrument and method::Oxygraph-2k]] [[Mammal and model::Annelids]] |?Mammal and model=Organism

...ategory:Publications]] [[Instrument and method::Oxygraph-2k]] [[Mammal and model::Crustaceans]] |?Mammal and model=Organism

...ategory:Publications]] [[Instrument and method::Oxygraph-2k]] [[Mammal and model::Other invertebrates]] |?Mammal and model=Organism

...ategory:Publications]] [[Instrument and method::Oxygraph-2k]] [[Mammal and model::Dog]] |?Mammal and model=Organism

...ategory:Publications]] [[Instrument and method::Oxygraph-2k]] [[Mammal and model::Rat]] |?Mammal and model=Organism

...ategory:Publications]] [[Instrument and method::Oxygraph-2k]] [[Mammal and model::Mouse]] |?Mammal and model=Organism

...ategory:Publications]] [[Instrument and method::Oxygraph-2k]] [[Mammal and model::Horse]] |?Mammal and model=Organism

...ategory:Publications]] [[Instrument and method::Oxygraph-2k]] [[Mammal and model::Pig]] |?Mammal and model=Organism

...ategory:Publications]] [[Instrument and method::Oxygraph-2k]] [[Mammal and model::Chicken]] |?Mammal and model=Organism

...ategory:Publications]] [[Instrument and method::Oxygraph-2k]] [[Mammal and model::Cat]] |?Mammal and model=Organism

...ategory:Publications]] [[Instrument and method::Oxygraph-2k]] [[Mammal and model::Rabbit]] |?Mammal and model=Organism

...ategory:Publications]] [[Instrument and method::Oxygraph-2k]] [[Mammal and model::Bovines]] |?Mammal and model=Organism

...aph-2k]] [[Mammal and model::Human]] [[Mammal and model::Mouse]] [[Tissue and cell::Skeletal muscle]] |?Mammal and model=Organism

...ategory:Publications]] [[Instrument and method::Oxygraph-2k]] [[Mammal and model::Guinea pig]] |?Mammal and model=Organism

...ategory:Publications]] [[Instrument and method::Oxygraph-2k]] [[Mammal and model::Saccharomyces cerevisiae]] |?Mammal and model=Organism

...elopmental biology and aging research and the intact organism is a perfect model for the O2k. ...ategory:Publications]] [[Instrument and method::Oxygraph-2k]] [[Mammal and model::Caenorhabditis elegans]]

»[[O2k-Publications: Chicken]] listed as '''model organism''' ...ategory:Publications]] [[Instrument and method::Oxygraph-2k]] [[Mammal and model::Birds]]

...ategory:Publications]] [[Instrument and method::Oxygraph-2k]] [[Mammal and model::Reptiles]] |?Mammal and model=Organism

...ategory:Publications]] [[Instrument and method::Oxygraph-2k]] [[Mammal and model::Artemia]] |?Mammal and model=Organism

...ategory:Publications]] [[Instrument and method::Oxygraph-2k]] [[Mammal and model::Amphibians]] |?Mammal and model=Organism

...ategory:Publications]] [[Instrument and method::Oxygraph-2k]] [[Mammal and model::Molluscs]] |?Mammal and model=Organism

...ategory:Publications]] [[Instrument and method::Oxygraph-2k]] [[Mammal and model::Zebrafish]] |?Mammal and model=Organism

...'Fishes''' such as bony fishes, carilaginous fishes (sharks, rays, skates) and jawless fishes (hagfish, lampreys); see also [[O2k-Publications: Zebrafish ...ategory:Publications]] [[Instrument and method::Oxygraph-2k]] [[Mammal and model::Fishes]]

...{#ask:[[Category:Publications]][[Was published in year::1993]][[Instrument and method::Oxygraph-2k]] {{#ask:[[Category:Publications]][[Was published in year::1993]][[Instrument and method::Oxygraph-2k]]

The word Nematoda comes from the Greek words ''nematos'', meaning thread, and ''eidos'', meaning form. ...us located near the tail. Nematodes possess digestive, nervous, excretory, and reproductive systems, but lack a discrete circulatory or respiratory system

|?Mammal and model=Organism |?Tissue and cell=Tissue;cell

|?Mammal and model=Organism |?Tissue and cell=Tissue;cell

...2k]] [[Injury and adaptation::Mitochondrial Disease; Degenerative Disease and Defect]] |?Tissue and cell=Tissue;cell

|?Mammal and model=Organism |?Tissue and cell=Tissue;cell

{{#ask:[[Category:Publications]] [[Instrument and method::Oxygraph-2k]] [[Additional label::Chlamydomonas]] |?Mammal and model=Organism

...tary intake of flavonoids as nutraceuticals is discussed for targeting T2D and other degenerative diseases. == Flavonoids and mitObesity ==

...les mitochondrial respiration. Rapamycin delays senescence in human cells, and extends lifespan in mice without detrimental effects on mitochondrial fitne == Rapamycin and mitObesity ==

...tion and anticancer immunosuppression by preserving mitochondrial function and control of autophagy. == Spermidine and mitObesity ==

|?Mammal and model=Organism |?Tissue and cell

...sage adjusted to individual body mass. It is a non-selective COX inhibitor and exerts an effect on long-chain fatty acid transport into mitochondria. == Aspirin and mitObesity ==

|?Respiration and regulation |?Mammal and model=Organism

|?Mammal and model=Organism |?Tissue and cell

...:[[Category:Publications]] [[Instrument and method::Oxygraph-2k]] [[Tissue and cell::Nervous system]] [[Pathways::F]] |?Mammal and model=Organism

{{#ask:[[Category:Abstracts]] [[Additional label::MiP2013]] [[Tissue and cell::Heart]] |?Mammal and model=Organism

|?Mammal and model |?Tissue and cell

|?Mammal and model |?Tissue and cell

{{#ask:[[Category:Abstracts]] [[Additional label::MiP2013]] [[Tissue and cell::Skeletal muscle]] |?Mammal and model=Organism

{{#ask:[[Category:Abstracts]] [[Additional label::MiP2013]] [[Tissue and cell::Nervous system]] |?Mammal and model=Organism

{{#ask:[[Category:Publications]] [[Instrument and method::Oxygraph-2k]] [[Coupling states::OXPHOS]] |?Mammal and model=Species;model

{{#ask:[[Category:Publications]] [[Instrument and method::Oxygraph-2k]] [[Coupling states::LEAK]] |?Mammal and model=Species;model

{{#ask:[[Category:Publications]] [[Instrument and method::Oxygraph-2k]] [[Coupling states::ROUTINE]] |?Mammal and model=Species;model

|?Mammal and model |?Tissue and cell

|?Mammal and model |?Tissue and cell

{{#ask:[[Category:Publications]] [[Instrument and method::Oxygraph-2k]] [[Coupling states::ET]] |?Mammal and model=Species;model

== Elamipretide and mitObesity == |?Mammal and model=Organism

...ategory:Publications]] [[Instrument and method::Oxygraph-2k]] [[Instrument and method::Ca]] |?Mammal and model=Organism

'''Chinese hamster ovary''' ('''CHO''') cells are a mammalian model cell line derived from the ovary of the Chinese hamster. ...:[[Category:Publications]] [[Instrument and method::Oxygraph-2k]] [[Tissue and cell::CHO]]

:::: This topic includes applications of the O2k-Fluo ([[CaG]]) and [[MiPNet15.08 TPP electrode |Ca²⁺-ISE]]. ...ategory:Publications]] [[Instrument and method::Oxygraph-2k]] [[Instrument and method::Ca]]

{{#ask:[[Category:Publications]] [[Instrument and method::Oxygraph-2k]] [[Topic::Calcium]] |?Mammal and model=Organism

...ategory:Publications]] [[Instrument and method::Oxygraph-2k]] [[Instrument and method::O2k-Spectrophotometer]] |?Mammal and model=Organism

...ategory:Publications]] [[Instrument and method::Oxygraph-2k]] [[Instrument and method::O2k-Protocol]] |?Mammal and model=Organism

{{#ask:[[Category:Publications]] [[Instrument and method::Oxygraph-2k]] [[Enzyme::Supercomplex]] |?Mammal and model=Organism

{{#ask:[[Category:Publications]] [[Instrument and method::Oxygraph-2k]] [[Topic::ADP]] |?Mammal and model=Organism

...:[[Category:Publications]] [[Instrument and method::Oxygraph-2k]] [[Injury and adaptation::Cell death]] |?Mammal and model=Organism

{{#ask:[[Category:Publications]] [[Instrument and method::Oxygraph-2k]] [[Substrate states::FAO]] |?Tissue and cell

...ategory:Publications]] [[Instrument and method::Oxygraph-2k]] [[Instrument and method::Oxygraph-2k]] |?Mammal and model=Organism

...ategory:Publications]] [[Instrument and method::Oxygraph-2k]] [[Instrument and method::NO]] |?Mammal and model=Organism

...any publication on bioenergetics using a gene knock-out or overexpression model. {{#ask:[[Category:Publications]] [[Instrument and method::Oxygraph-2k]] [[MiP area::Genetic knockout;overexpression]]

...:[[Category:Publications]] [[Instrument and method::Oxygraph-2k]] [[Tissue and cell::Kidney]] |?Mammal and model=Organism

...:[[Category:Publications]] [[Instrument and method::Oxygraph-2k]] [[Tissue and cell::Liver]] |?Mammal and model=Organism

...:[[Category:Publications]] [[Instrument and method::Oxygraph-2k]] [[Tissue and cell::Fat]] |?Mammal and model=Organism

...:[[Category:Publications]] [[Instrument and method::Oxygraph-2k]] [[Tissue and cell::Genital]] |?Mammal and model=Organism

{{#ask:[[Category:Publications]] [[Instrument and method::Oxygraph-2k]] [[Topic::Q-junction effect]] |?Mammal and model=Organism

...:[[Category:Publications]] [[Instrument and method::Oxygraph-2k]] [[Injury and adaptation::Hypoxia]] |?Mammal and model=Organism

{{#ask:[[Category:Publications]] [[Instrument and method::Oxygraph-2k]] [[Enzyme::Marker enzyme]] |?Mammal and model=Organism

{{#ask:[[Category:Publications]] [[Instrument and method::Oxygraph-2k]] [[Topic::Fatty acid]] |?Mammal and model=Organism

|?Mammal and model=Organism |?Tissue and cell=Tissue;cell

{{#ask:[[Category:Publications]] [[Instrument and method::Oxygraph-2k]] [[Enzyme::Complex III]] |?Mammal and model=Organism

{{#ask:[[Category:Publications]] [[Instrument and method::Oxygraph-2k]] [[Enzyme::Uncoupling protein]] |?Mammal and model=Organism

{{#ask:[[Category:Publications]] [[Instrument and method::Oxygraph-2k]] [[Topic::ATP production]] |?Mammal and model=Organism

{{#ask:[[Category:Publications]] [[Instrument and method::Oxygraph-2k]] [[Topic::Aerobic glycolysis]] |?Mammal and model=Organism

{{#ask:[[Category:Publications]] [[Instrument and method::Oxygraph-2k]] [[Topic::Amino acid]] |?Mammal and model=Organism

{{#ask:[[Category:Publications]] [[Instrument and method::Oxygraph-2k]] [[Topic::Cyt c]] |?Mammal and model=Organism

{{#ask:[[Category:Publications]] [[Instrument and method::Oxygraph-2k]] [[Topic::Redox state]] |?Mammal and model=Organism

...:[[Category:Publications]] [[Instrument and method::Oxygraph-2k]] [[Tissue and cell::Heart]] |?Mammal and model=Organism

|?Mammal and model=Organism |?Tissue and cell=Tissue;cell

...:Publications]] [[Instrument and method::Oxygraph-2k]] [[Enzyme::TCA cycle and matrix dehydrogenases]] |?Mammal and model=Organism

{{#ask:[[Category:Publications]] [[Instrument and method::Oxygraph-2k]] [[Additional label::PBI-Shredder]] |?Mammal and model=Organism

...:[[Category:Publications]] [[Instrument and method::Oxygraph-2k]] [[Tissue and cell::Lymphocyte]] |?Mammal and model=Organism

{{#ask:[[Category:Publications]] [[Instrument and method::Oxygraph-2k]] [[Enzyme::Complex I]] |?Mammal and model=Organism

{{#ask:[[Category:Publications]] [[Instrument and method::Oxygraph-2k]] [[Additional label::MgG]] |?Mammal and model=Organism

{{#ask:[[Category:Publications]] [[Instrument and method::Oxygraph-2k]] [[Additional label::CaG]] |?Mammal and model=Organism

{{#ask:[[Category:Publications]] [[Instrument and method::Oxygraph-2k]] [[Additional label::AmR]] |?Mammal and model=Organism

{{#ask:[[Category:Publications]] [[Instrument and method::Oxygraph-2k]] [[Additional label::Photosynthesis]] |?Mammal and model=Organism

{{#ask:[[Category:Publications]] [[Instrument and method::Oxygraph-2k]] [[Additional label::PBMCs]] |?Mammal and model=Organism

...:[[Category:Publications]] [[Instrument and method::Oxygraph-2k]] [[Tissue and cell::Platelet]] |?Mammal and model=Organism

...ategory:Publications]] [[Instrument and method::Oxygraph-2k]] [[Instrument and method::TIP2k]] |?Mammal and model=Organism

{{#ask:[[Category:Publications]] [[Instrument and method::Oxygraph-2k]] [[Topic::Substrate]] |?Mammal and model=Organism

{{#ask:[[Category:Publications]] [[Instrument and method::Oxygraph-2k]] [[Topic::mt-Membrane potential]] |?Mammal and model=Organism

{{#ask:[[Category:Publications]] [[Instrument and method::Oxygraph-2k]] [[Additional label::Tissue normoxia]] |?Mammal and model=Organism

...:[[Category:Publications]] [[Instrument and method::Oxygraph-2k]] [[Tissue and cell::Lung;gill]] |?Mammal and model=Organism

{{#ask:[[Category:Publications]] [[Instrument and method::Oxygraph-2k]] [[Enzyme::Adenine nucleotide translocase]] |?Mammal and model=Organism

{{#ask:[[Category:Publications]] [[Instrument and method::Oxygraph-2k]] [[Additional label::CaG]] |?Mammal and model=Organism

'''Property: Regulation and kinetics''' {{#ask:[[Category:Publications]] [[Instrument and method::Oxygraph-2k]] [[Topic::Uncoupler]]

...:[[Category:Publications]] [[Instrument and method::Oxygraph-2k]] [[Tissue and cell::Blood cells]] |?Mammal and model=Organism

{{#ask:[[Category:Publications]] [[Instrument and method::Oxygraph-2k]] [[Preparation::Homogenate]] |?Mammal and model=Organism

{{#ask:[[Category:Publications]] [[Instrument and method::Oxygraph-2k]] [[Preparation::SMP]] |?Mammal and model=Organism

{{#ask:[[Category:Publications]] [[Instrument and method::Oxygraph-2k]] [[Preparation::Chloroplasts]] |?Mammal and model=Organism

{{#ask:[[Category:Publications]] [[Instrument and method::Oxygraph-2k]] [[Preparation::Enzyme]] |?Mammal and model=Organism

{{#ask:[[Category:Publications]] [[Instrument and method::Oxygraph-2k]] [[Topic::Inhibitor]] |?Mammal and model=Organism

...:[[Category:Publications]] [[Instrument and method::Oxygraph-2k]] [[Injury and adaptation::Cryopreservation]] |?Mammal and model=Organism

{{#ask:[[Category:Publications]] [[Instrument and method::Oxygraph-2k]] [[Enzyme::Inner mt-membrane transporter]] |?Mammal and model=Organism

{{#ask:[[Category:Publications]] [[Instrument and method::Oxygraph-2k]] [[Topic::Ion;substrate transport]] |?Mammal and model=Organism

{{#ask:[[Category:Publications]] [[Instrument and method::Oxygraph-2k]] [[Topic::Threshold;excess capacity]] |?Mammal and model=Organism

{{#ask:[[Category:Publications]] [[Instrument and method::Oxygraph-2k]] [[Enzyme::Complex II;succinate dehydrogenase]] |?Mammal and model=Organism

|?Mammal and model=Organism |?Tissue and cell=Tissue;cell

{{#ask:[[Category:Publications]] [[Instrument and method::Oxygraph-2k]] [[Additional label::PBMC]] |?Mammal and model=Organism

{{#ask:[[Category:Publications]] [[Instrument and method::Oxygraph-2k]] [[Additional label::Leukemia]] |?Mammal and model=Organism

...ategory:Publications]] [[Instrument and method::Oxygraph-2k]] [[Instrument and method::O2k-Fluorometer]] |?Mammal and model=Organism

{{#ask:[[Category:Publications]] [[Instrument and method::Oxygraph-2k]] [[MiP area::Comparative MiP;environmental MiP]] |?Mammal and model=Organism

...:[[Category:Publications]] [[Instrument and method::Oxygraph-2k]] [[Tissue and cell::Stem cells]] |?Mammal and model=Organism

{{#ask:[[Category:Publications]] [[Instrument and method::O2k-Protocol]] [[Additional label::O2k-Demo]] [[Additional label::O |?Tissue and cell

...:[[Category:Publications]] [[Instrument and method::Oxygraph-2k]] [[Tissue and cell::Nervous system]] |?Mammal and model=Organism

...:[[Category:Publications]] [[Instrument and method::Oxygraph-2k]] [[Tissue and cell::Islet cell;pancreas;thymus]] |?Mammal and model=Organism

...on and regulation]]''' - Oxygen kinetics, oxygen dependence of respiration and other mitochondrial functions ...tegory:Publications]] [[Instrument and method::Oxygraph-2k]] [[Respiration and regulation::Oxygen kinetics]]

{{#ask:[[Category:Publications]] [[Instrument and method::Oxygraph-2k]] [[Preparation::Isolated mitochondria]] |?Mammal and model=Organism

{{#ask:[[Category:Publications]] [[Instrument and method::Oxygraph-2k]] [[Preparation::Intact organism]] |?Mammal and model=Organism

{{#ask:[[Category:Publications]] [[Instrument and method::Oxygraph-2k]] [[Diseases::Other]] |?Mammal and model=Organism

...:[[Category:Publications]] [[Instrument and method::Oxygraph-2k]] [[Tissue and cell::Skeletal muscle]] |?Mammal and model=Organism

{{#ask:[[Category:Publications]] [[Instrument and method::Oxygraph-2k]] [[Topic::Flux control]] |?Mammal and model=Organism

{{#ask:[[Category:Publications]] [[Instrument and method::Oxygraph-2k]] [[Enzyme::Complex V;ATP synthase]] |?Mammal and model=Organism

...:[[Category:Publications]] [[Instrument and method::Oxygraph-2k]] [[Injury and adaptation::Temperature]] |?Mammal and model=Organism

{{#ask:[[Category:Publications]] [[Instrument and method::Oxygraph-2k]] [[Preparation::Intact organ]] |?Mammal and model=Organism

{{#ask:[[Category:Publications]] [[Instrument and method::Oxygraph-2k]] [[MiP area::Pharmacology;toxicology]] |?Mammal and model=Organism

...iginal kidney culture. HEK 293 cells should not be used as an ''in vitro'' model for kidney cell function or studies involving kidney cells. ...:[[Category:Publications]] [[Instrument and method::Oxygraph-2k]] [[Tissue and cell::HEK]]

...:[[Category:Publications]] [[Instrument and method::Oxygraph-2k]] [[Tissue and cell::Endothelial;epithelial;mesothelial cell]] |?Mammal and model=Organism

...ns have specific features and characteristics like the presence of keratin and resistance to polioviruses. ...:[[Category:Publications]] [[Instrument and method::Oxygraph-2k]] [[Tissue and cell::HeLa]]

{{#ask:[[Category:Publications]] [[Instrument and method::Oxygraph-2k]] [[Diseases::Infectious]] |?Mammal and model=Organism

{{#ask:[[Category:Publications]] [[Instrument and method::Oxygraph-2k]] [[Diseases::Inherited]] |?Mammal and model=Organism

{{#ask:[[Category:Publications]] [[Instrument and method::Oxygraph-2k]] [[Diseases::Myopathy]] |?Mammal and model=Organism

{{#ask:[[Category:Publications]] [[Instrument and method::Oxygraph-2k]] [[Topic::Coupling efficiency;uncoupling]] |?Mammal and model=Organism

{{#ask:[[Category:Publications]] [[Instrument and method::Oxygraph-2k]] [[Diseases::Diabetes]] |?Mammal and model=Organism

{{#ask:[[Category:Publications]] [[Instrument and method::Oxygraph-2k]] [[Diseases::COPD]] |?Mammal and model=Organism

{{#ask:[[Category:Publications]] [[Instrument and method::Oxygraph-2k]] [[Diseases::Sepsis]] |?Mammal and model=Organism

{{#ask:[[Category:Publications]] [[Instrument and method::Oxygraph-2k]] [[Diseases::Autism]] |?Mammal and model=Organism

{{#ask:[[Category:Publications]] [[Instrument and method::Oxygraph-2k]] [[Diseases::Neurodegenerative]] |?Mammal and model=Organism

{{#ask:[[Category:Publications]] [[Instrument and method::Oxygraph-2k]] [[Enzyme::Complex IV;cytochrome c oxidase]] |?Mammal and model=Organism

{{#ask:[[Category:Publications]] [[Instrument and method::Oxygraph-2k]] [[Preparation::Oxidase;biochemical oxidation]] |?Mammal and model=Organism

...ions]] [[Organism::Human]] [[Instrument and method::Oxygraph-2k]] [[Tissue and cell type::Heart]] |?Mammal and model=Organism

{{#ask:[[Category:Publications]] [[Instrument and method::Oxygraph-2k]] [[Preparation::Intact cells]] |?Mammal and model=Organism

...:[[Category:Publications]] [[Instrument and method::Oxygraph-2k]] [[Injury and adaptation::Permeability transition]] |?Mammal and model=Organism

{{#ask:[[Category:Publications]] [[Instrument and method::Oxygraph-2k]] [[Diseases::Obesity]] |?Mammal and model=Organism

{{#ask:[[Category:Publications]] [[Instrument and method::Oxygraph-2k]] [[Topic::Temperature]] |?Mammal and model=Organism

{{#ask:[[Category:Publications]] [[Instrument and method::Oxygraph-2k]] [[Diseases::Cancer]] |?Mammal and model=Organism

...ions]] [[Organism::Human]] [[Instrument and method::Oxygraph-2k]] [[Tissue and cell type::Skeletal muscle]] |?Mammal and model=Organism

For specific chapters, see [[O2k-Procedures]] and [[O2k-Manual]]. {{#ask:[[Category:Publications]] [[Instrument and method::O2k-Protocol]]

{{#ask:[[Category:Publications]] [[Instrument and method::Oxygraph-2k]] [[Diseases::Aging;senescence]] |?Mammal and model=Organism

{{#ask:[[Category:Publications]] [[Instrument and method::Oxygraph-2k]] [[Diseases::Parkinson's]] |?Mammal and model=Organism

# [[Property:Mammal and model]] # [[Property:Tissue and cell]]

{{#ask:[[Category:Publications]] [[Instrument and method::Oxygraph-2k]] [[Diseases::Cardiovascular]] |?Mammal and model=Organism

...:[[Category:Publications]] [[Instrument and method::Oxygraph-2k]] [[Tissue and cell::Skeletal muscle]] [[Preparation::Permeabilized tissue]] |?Mammal and model=Organism

...:[[Category:Publications]] [[Instrument and method::Oxygraph-2k]] [[Injury and adaptation::Ischemia-reperfusion]] |?Mammal and model=Organism

...:[[Category:Publications]] [[Instrument and method::Oxygraph-2k]] [[Injury and adaptation::Oxidative stress;RONS]] |?Mammal and model=Organism

{{#ask:[[Category:Publications]] [[Instrument and method::Oxygraph-2k]] [[MiP area::mt-Medicine]] |?Mammal and model=Organism

{{#ask:[[Category:Publications]] [[Instrument and method::Oxygraph-2k]] [[Pathways::ROX]] |?Mammal and model=Organism

{{#ask:[[Category:Publications]] [[Instrument and method::Oxygraph-2k]] [[Pathways::CIV]] |?Mammal and model=Organism

{{#ask:[[Category:Publications]] [[Instrument and method::Oxygraph-2k]] [[Diseases::Alzheimer's]] |?Mammal and model=Organism

{{#ask:[[Category:Publications]] [[Instrument and method::Oxygraph-2k]] [[Pathways::Gp]] |?Mammal and model=Organism

...tissue''', including [[permeabilized muscle fibers]]: selective mechanical and chemical ([[saponin]]) permeabilization of the plasma membrane. {{#ask:[[Category:Publications]] [[Instrument and method::Oxygraph-2k]] [[Preparation::Permeabilized tissue]]

{{#ask:[[Category:Publications]] [[Instrument and method::Oxygraph-2k]] [[Pathways::F]] |?Mammal and model=Organism

{{#ask:[[Category:Publications]] [[Instrument and method::Oxygraph-2k]] [[Pathways::N]] |?Mammal and model=Organism

{{#ask:[[Category:Publications]] [[Instrument and method::Oxygraph-2k]] [[Pathways::DQ]] |?Mammal and model=Organism

{{#ask:[[Category:Publications]] [[Instrument and method::Oxygraph-2k]] [[Pathways::NS]] |?Mammal and model=Organism

{{#ask:[[Category:Publications]] [[Instrument and method::Oxygraph-2k]] [[Pathways::Other combinations]] |?Mammal and model=Organism

{{#ask:[[Category:Publications]] [[Instrument and method::Oxygraph-2k]] [[Diseases::Inherited]] OR [[Stress::Mitochondrial di |?Mammal and model=Organism

...physiological processes, including arterial disease and cancer development and other endothelial cell signaling pathways). ...:[[Category:Publications]] [[Instrument and method::Oxygraph-2k]] [[Tissue and cell::HUVEC]]

{{#ask:[[Category:Publications]] [[Instrument and method::Oxygraph-2k]] [[Pathways::S]] |?Mammal and model=Organism

...{#ask:[[Category:Publications]][[Was published in year::1997]][[Instrument and method::Oxygraph-2k]] {{#ask:[[Category:Publications]][[Was published in year::1997]][[Instrument and method::Oxygraph-2k]]

...{#ask:[[Category:Publications]][[Was published in year::1996]][[Instrument and method::Oxygraph-2k]] {{#ask:[[Category:Publications]][[Was published in year::1996]][[Instrument and method::Oxygraph-2k]]

...{#ask:[[Category:Publications]][[Was published in year::1995]][[Instrument and method::Oxygraph-2k]] {{#ask:[[Category:Publications]][[Was published in year::1995]][[Instrument and method::Oxygraph-2k]]

...{#ask:[[Category:Publications]][[Was published in year::1994]][[Instrument and method::Oxygraph-2k]] {{#ask:[[Category:Publications]][[Was published in year::1994]][[Instrument and method::Oxygraph-2k]]

{{#ask:[[Category:Publications]] [[Instrument and method::Oxygraph-2k]] [[MiP area::mt-Biogenesis;mt-density]] |?Mammal and model=Organism

{{#ask:[[Category:Publications]] [[Instrument and method::O2k-Fluorometer]] |?Mammal and model=Organism

...{#ask:[[Category:Publications]][[Was published in year::2010]][[Instrument and method::Oxygraph-2k]] {{#ask:[[Category:Publications]][[Was published in year::2010]][[Instrument and method::Oxygraph-2k]]

...{#ask:[[Category:Publications]][[Was published in year::2009]][[Instrument and method::Oxygraph-2k]] {{#ask:[[Category:Publications]][[Was published in year::2009]][[Instrument and method::Oxygraph-2k]]

...{#ask:[[Category:Publications]][[Was published in year::2008]][[Instrument and method::Oxygraph-2k]] {{#ask:[[Category:Publications]][[Was published in year::2008]][[Instrument and method::Oxygraph-2k]]

...{#ask:[[Category:Publications]][[Was published in year::2007]][[Instrument and method::Oxygraph-2k]] {{#ask:[[Category:Publications]][[Was published in year::2007]][[Instrument and method::Oxygraph-2k]]

...{#ask:[[Category:Publications]][[Was published in year::2006]][[Instrument and method::Oxygraph-2k]] {{#ask:[[Category:Publications]][[Was published in year::2006]][[Instrument and method::Oxygraph-2k]]

...{#ask:[[Category:Publications]][[Was published in year::2005]][[Instrument and method::Oxygraph-2k]] {{#ask:[[Category:Publications]][[Was published in year::2005]][[Instrument and method::Oxygraph-2k]]

...{#ask:[[Category:Publications]][[Was published in year::2004]][[Instrument and method::Oxygraph-2k]] {{#ask:[[Category:Publications]][[Was published in year::2004]][[Instrument and method::Oxygraph-2k]]

...{#ask:[[Category:Publications]][[Was published in year::2003]][[Instrument and method::Oxygraph-2k]] {{#ask:[[Category:Publications]][[Was published in year::2003]][[Instrument and method::Oxygraph-2k]]

...{#ask:[[Category:Publications]][[Was published in year::2002]][[Instrument and method::Oxygraph-2k]] {{#ask:[[Category:Publications]][[Was published in year::2002]][[Instrument and method::Oxygraph-2k]]

...{#ask:[[Category:Publications]][[Was published in year::2001]][[Instrument and method::Oxygraph-2k]] {{#ask:[[Category:Publications]][[Was published in year::2001]][[Instrument and method::Oxygraph-2k]]

...{#ask:[[Category:Publications]][[Was published in year::2000]][[Instrument and method::Oxygraph-2k]] {{#ask:[[Category:Publications]][[Was published in year::2000]][[Instrument and method::Oxygraph-2k]]

...{#ask:[[Category:Publications]][[Was published in year::1999]][[Instrument and method::Oxygraph-2k]] {{#ask:[[Category:Publications]][[Was published in year::1999]][[Instrument and method::Oxygraph-2k]]

...{#ask:[[Category:Publications]][[Was published in year::1998]][[Instrument and method::Oxygraph-2k]] {{#ask:[[Category:Publications]][[Was published in year::1998]][[Instrument and method::Oxygraph-2k]]

...{#ask:[[Category:Publications]][[Was published in year::2011]][[Instrument and method::Oxygraph-2k]] {{#ask:[[Category:Publications]][[Was published in year::2011]][[Instrument and method::Oxygraph-2k]]

...{#ask:[[Category:Publications]][[Was published in year::2012]][[Instrument and method::Oxygraph-2k]] {{#ask:[[Category:Publications]][[Was published in year::2012]][[Instrument and method::Oxygraph-2k]]

'''{{#ask:[[Category:Publications]][[Instrument and method::Oxygraph-2k]][[Preparation::Permeabilized cells]] |format=count}} O {{#ask:[[Category:Publications]] [[Instrument and method::Oxygraph-2k]] [[Preparation::Permeabilized cells]]

...ent [[mitochondrial preparations]], as a guide to select the best approach and to critically evaluate published results. |?Mammal and model=Organism

...culturing, they adhere and function as scavengers of modified lipoproteins and dead apoptotic cells. They also produce growth factors. ...:[[Category:Publications]] [[Instrument and method::Oxygraph-2k]] [[Tissue and cell::Macrophage-derived]]

{{#ask:[[Category:Publications]] [[Instrument and method::Oxygraph-2k]] [[MiP area::Exercise physiology;nutrition;life style] |?Mammal and model=Organism

...{#ask:[[Category:Publications]][[Was published in year::2013]][[Instrument and method::Oxygraph-2k]] {{#ask:[[Category:Publications]][[Was published in year::2013]][[Instrument and method::Oxygraph-2k]]

...{#ask:[[Category:Publications]][[Was published in year::2014]][[Instrument and method::Oxygraph-2k]] {{#ask:[[Category:Publications]][[Was published in year::2014]][[Instrument and method::Oxygraph-2k]]

...{#ask:[[Category:Publications]][[Was published in year::2015]][[Instrument and method::Oxygraph-2k]] {{#ask:[[Category:Publications]][[Was published in year::2015]][[Instrument and method::Oxygraph-2k]]

...{#ask:[[Category:Publications]][[Was published in year::2016]][[Instrument and method::Oxygraph-2k]] {{#ask:[[Category:Publications]][[Was published in year::2016]][[Instrument and method::Oxygraph-2k]]

...{#ask:[[Category:Publications]][[Was published in year::2017]][[Instrument and method::Oxygraph-2k]] {{#ask:[[Category:Publications]][[Was published in year::2017]][[Instrument and method::Oxygraph-2k]]

...{#ask:[[Category:Publications]][[Was published in year::2018]][[Instrument and method::Oxygraph-2k]] {{#ask:[[Category:Publications]][[Was published in year::2018]][[Instrument and method::Oxygraph-2k]]

...{#ask:[[Category:Publications]][[Was published in year::2019]][[Instrument and method::Oxygraph-2k]] {{#ask:[[Category:Publications]][[Was published in year::2019]][[Instrument and method::Oxygraph-2k]]

...{#ask:[[Category:Publications]][[Was published in year::2020]][[Instrument and method::Oxygraph-2k]] {{#ask:[[Category:Publications]][[Was published in year::2020]][[Instrument and method::Oxygraph-2k]]

...ratio''' is the ratio of (partially coupled) [[ROUTINE respiration]] ''R'' and (noncoupled) [[ET capacity]] ''E''. The ''R/E'' control ratio is an expres ...'E'' by limitation of oxidative capacity by defects of substrate oxidation and complexes of the ET pathway. The corresponding inverse ratio is the uncoupl

...ulture after about 20-30 generations of ''in vitro'' growth. George Todaro and Howard Green, the scientists who first cultured this cell line, obtained th ...:[[Category:Publications]] [[Instrument and method::Oxygraph-2k]] [[Tissue and cell::Fibroblast]]

{{MitoPedia O2k and high-resolution respirometry}} ::::* A MiPNet describing and comparing various blood cell isolation protocols can be found here: [[MiPNe

== List of publications: OXPHOS and ET pathway == ::: '''» OXPHOS and ET-pathway'''

...ET capacity]], as determined by measurement of oxygen consumption in ''L'' and ''E'' sequentially. The ''L/E'' coupling-control ratio is an index of [[unc :::# Gnaiger E (2020) Mitochondrial pathways and respiratory control. An introduction to OXPHOS analysis. 5th ed. Bioenerg C

|?Mammal and model=Organism |?Tissue and cell=Tissue;cell

...tions''', which is updated by [[Bioblast editorial team|Bioblast editors]] and [[Special:CreateAccount|active wiki users]]. → '''Contact: [mailto:mario. '''Number of O2k-Publications: {{#ask:[[Category:Publications]][[Instrument and method::Oxygraph-2k]] |format=count

...zes the reduction of cytochrome ''c'' by oxidation of [[coenzyme Q]] (CoQ) and the concomitant [[Proton pump|pumping of 4 protons]] from the cathodic (neg |?Mammal and model

|?Mammal and model=Organism |?Tissue and cell=Tissue;cell

...euroblastoma. Other human neuroblastoma cell lines: LAN-1, IMR-32 (N-type) and SK-N-AS (S-type). Mouse neuroblastoma cell line: Neuro 2a. ...:[[Category:Publications]] [[Instrument and method::Oxygraph-2k]] [[Tissue and cell::Neuroblastoma]]

...the effects of coupling (''L/E''), physiological control of energy demand, and limitation by the OXPHOS capacity. = List of publications: LEAK and ROUTINE =

...ases are commonly obesity-induced. The search for the link between obesity and the etiology of diverse preventable diseases lead to the hypothesis, that m |info=[[:Category:BME_and_mitObesity |Body mass excess and mitObesity]]

|?Mammal and model |?Tissue and cell

|?Mammal and model=Organism |?Tissue and cell=Tissue;cell

{{#ask:[[Category:Publications]] [[Instrument and method::Oxygraph-2k]] [[Nonmammal::Plants]] {{#ask:[[Category:Abstracts]] [[Instrument and method::Oxygraph-2k]] [[Nonmammal::Plants]]

...' is a natural bioactive phenol prouced by several plants with antioxidant and anti-inflammatory effects. Dietary intake as nutraceutical is discussed for == Resveratrol and mitObesity ==

'''O2k-Workshops with OROBOROS and Sunpoint Scientific Instrument Co., Ltd.''' |?Mammal and model=Organism

...K/OXPHOS coupling-control ratio combines the effects of coupling (''L/E'') and limitation by the phosphorylation system (''P/E''); ''L/P'' = (''L/E'') / ( = List of publications: LEAK and OXPHOS =

...ral limitations of blebbistatin i.e. low solubility in water, cytotoxicity and phototoxicity have been described. |?Mammal and model=Organism

...h the pool of redox intermediates in the pathway from CII to CIII and CIV, and if the capacity of the phosphorylation system does not limit OXPHOS capacit = List of publications: N and S and NS (CI and CII and CI<small>&</small>II) =

...ic GpDH. CGpDH oxidizes [[glycerophosphate]] to dihydroxyacetone phosphate and feeds two electrons into the [[Q-junction]], thus linked to an [[Electron-t |?Mammal and model

...es the [[O2k-Core]], [[O2k-Fluo LED2-Module]] and [[TIP2k-Module |TIP2k]], and supports all other add-on O2k-Modules of the [[Oroboros O2k]]. ...ategory:Publications]] [[Instrument and method::Oxygraph-2k]] [[Instrument and method::O2k-Fluorometer]]

...stimation of [[LEAK respiration]] measured in the absence of ADP, ''L''(n) and subsequent inhibition of respiration by oligomycin, ''L''(Omy). ...er] was developed for searching for the best SUIT protocols to answer this and other research questions.

.... NS indicates respiration with a cocktail of substrates supporting the N- and S-pathways. = List of publications: N and NS pathway control state =

Communicated by [[Huete-Ortega M]] and [[Gnaiger E]] (last update 2021-03-23) == Strengths and limitations ==

...regularly posted on the [[OROBOROS_INSTRUMENTS|Oroboros Ecosystem Agenda]] and disseminated in the [[Bioblast_alert |Bioblast alert circulars]]. :::: Oroboros supports the freedom to share information and chooses to highlight O2k-Publications with open-access [[Creative Commons A

|?Mammal and model=Organism |?Tissue and cell=Tissue;cell

|?Mammal and model |?Tissue and cell

Communicated by [[Huete-Ortega M]] and [[Gnaiger E]] (last update 2021-03-23) == Strengths and limitations ==

...:[[Category:Publications]] [[Instrument and method::Oxygraph-2k]] [[Tissue and cell::Other cell lines]] |?Mammal and model=Organism

...ategory:Publications]] [[Instrument and method::Oxygraph-2k]] [[Instrument and method::TIP2k]] |?Mammal and model=Organism

...px|link=http://www.iubmb.org|IUBMB - ''International Union of Biochemistry and Molecular Biology'']] ...>Joint IUBMB/MiP Symposium on Mitochondrial Physiology - Methods, Concepts and Biomedical Perspectives</big></big>'''

...tases, FRDABCD) tend to operate in the reverse direction reducing fumarate and oxidizing quinol. |?Mammal and model

|?Mammal and model=Organism |?Tissue and cell=Tissue;cell

...e rate ''R''. The background state is the [[LEAK respiration|LEAK]] state, and the flux control variable is stimulation to ROUTINE respiration by physiolo == List of publications: ROUTINE and LEAK respiration ==

= List of publications: S and NS pathway control states = |?Mammal and model

: » '''[[MiP2015]]''' - all abstracts in alphabetical order and programme sessions. == MiPMap - Comparative: species, tissue and cells, preparation ==

== MiPMap - Comparative: species, tissue and cells, preparation == |?Mammal and model

: » '''[[MiP2014]]''' - all abstracts in alphabetical order and programme sessions. == MiPMap - Comparative: species, tissue and cells, preparation ==

== MiPMap - Comparative: species, tissue and cells, preparation == |?Mammal and model

...ling-control protocol, CCP'''<ref> Gnaiger E (2020) Mitochondrial pathways and respiratory control. An introduction to OXPHOS analysis. 5th ed. BEC 2020.2 ...iger E (2014) Flux control ratios in isolatd mitochondria. OXPHOS capacity and respiratory control in isolated mitochondria. Mitochondr Physiol Network 12

...ux in mitochondrial preparations (isolated mitochondria, tissue homogenate and permeabilized cells) at low oxygen concentration (tissue normoxia) ...otocol to study simultaneously the oxygen dependence of O₂ flux and H₂O₂ production on mitochondrial preparations. Is it

== Manuscript table and figure blocks == ::::* Section: Workflow # and Title

...ategory:Publications]] [[Instrument and method::Oxygraph-2k]] [[Instrument and method::TPP]] ...[[Category:Abstracts]] [[Instrument and method::Oxygraph-2k]] [[Instrument and method::TPP]]

..., 2016 Dec 16-17. Mito Xmas Meeting 2016. Mitochondrial function in health and disease. Mitochondr Physiol Network 21.03. |abstract=Mito Xmas Meeting 2016. Mitochondrial function in health and disease. Innsbruck, AT.

...CI<small>&</small>II as the measured flux in the presence of both CI- '''and''' CII-linked substrates from CI+CII as the operation of a mathematical ad == List of publications: NS and N pathway control state ==

...ications, which is updated by [[Bioblast editorial team|Bioblast editors]] and [[Special:CreateAccount|active wiki users]].''' → Contact: [mailto:mario. ...ublications - chronological: {{#ask:[[Category:Publications]][[Instrument and method::Oxygraph-2k]] |format=count

...er with either ROS production (AmR), mt-membrane potential (TMRM, Safranin and Rhodamine 123), Ca²⁺ (CaG) or ATP production (MgG). ...www.oroboros.at/index.php/product/o2k-fluorespirometer/ '''Product details and purchase information''']

...ux in mitochondrial preparations (isolated mitochondria, tissue homogenate and permeabilized cells)- '''[[SUIT-032]]''' == Strengths and limitations ==

...x in mitochondrial preparations (isolated mitochondria, tissue homogenate and permeabilized cells)- '''[[SUIT-034]]''' == Strengths and limitations ==

...ux in mitochondrial preparations (isolated mitochondria, tissue homogenate and permeabilized cells)- '''[[SUIT-006]]''' == Strengths and limitations ==

Communicated by [[Huete-Ortega M]] and [[Gnaiger E]] (last update 2021-03-23) == Strengths and limitations ==

...ux in mitochondrial preparations (isolated mitochondria, tissue homogenate and permeabilized cells)- '''[[SUIT-033]]''' == Strengths and limitations ==

Communicated by [[Cardoso LHD]], [[Antunes D]] and [[Gnaiger E]] (last update 2019-07-31) == Strengths and limitations ==

...proteins that can be found in the inner mitochondrial membranes of animals and plants. [[Uncoupling protein 1 |UCP1]] acts as an [[uncoupler]] by dissipat ...n elicited by cold exposure, including ROS and lipid metabolism, apoptosis and thermogenesis.<ref>Criscuolo F, Gonzalez‐Barroso MdM, Bouillaud F, Ricqui

...ub>2</sub>O₂ (AmR) for isolated mitochondria, tissue homogenate and permeabilized cells ...H₂O₂ production) changes over the experimental time and upon addition of chemicals. To correct H₂O₂ flux for

...o may be larger or smaller than 1.0, depending on the mitochondrial source and various mitochondrial injuries. The S-pathway control state may be selected ...h a wide scatter between studies but a consistent correlation between PM/S and GM/S flux control ratios (PM''_P'' ~ GM''_P''). Low N/S

...aneously O₂ flux and the Q-redox state in isolated mitochondria and permeabilized cells. ...ation of O₂ flux and the Q-redox state in isolated mitochondria and cells permeabilized previously to addition to the O2k-chamber.

...typical [[SUIT protocol]]s with [[Electron-transfer-pathway state |type N and S substrates]], flux in the [[NS-pathway control state]] NS is inhibited by == List of publications: NS and S ==

...in the [[ET-excess control efficiency E-P]] in different species, tissues and cell lines. ...ogical substrate combinations (''P/E''<1.0), in contrast to mouse skeletal and cardiac tissue (''P/E''=0.97 to 1.0). Therefore, uncoupler titrations (to e

Communicated by [[Cardoso LH]], [[Komlodi T]] and [[Gnaiger E]] (last update 2019-07-03) == Strengths and limitations ==

Communicated by [[Garcia-Souza LF]], [[Komlodi T]] and [[Gnaiger E]](last update 2019-06-26) == Strengths and limitations ==

...day Sep 6''' will enjoy a social programme with a visit of the city centre and a boat trip. Transfer by bus from Prague to the conference venue will be ar ::::D. Mitochondrial physiology in life and death of the cell – regulation of mitochondrial energy provision signalli

== Strengths and limitations == ...the following protocols are recommended: [[SUIT 003 AmR ce D058]] with AmR and [[SUIT 003 AmR ce D059]] with carrier titration as a control. These two pro

...al in mitochondrial preparations (isolated mitochondria, tissue homogenate and permeabilized cells) -'''[[SUIT-006]]''' ...bilized when they are added to the chamber) in a wide variety of organisms and tissues.

Communicated by [[Doerrier C]], [[Komlodi T]], [[Huete-Ortega M]] and [[Gnaiger E]] (last update 2019-06-05) == Strengths and limitations ==

...> (the lonely [[hydron]])' surrounding Complex I: [[Ambiguity crisis - NAD and H+ |CI ambiguities]]. |?Mammal and model

Communicated by [[Komlodi T]] and [[Gnaiger E]] (last update 2021-11-09) == Strengths and limitations ==

...in mitochondrial preparations (isolated mitochondria, permeabilized cells, and tissue homogenate)- '''[[SUIT-020]]''' Communicated by [[Huete-Ortega M]], [[Antunes D]], [[Komlodi T]] and [[Gnaiger E]] (last update 2019-07-25)

== Strengths and limitations == ...llows to analyse the presence of ATPases in [[mitochondrial preparations]] and is useful as a quality control of the sample preparation.

...ry to determine CIV activity, in which case the instrumental O2 background and chemical background may be combined in an overall correction term. |?Mammal and model=Organism

Communicated by [[Komlodi T]], [[Garcia-Souza LF]] and [[Gnaiger E]] (last update 2019-06-24) == Strengths and limitations ==

|info='''A: simultaneous determination of O2 flux and mt-membrane potential''' ...pathway control state| NS-pathway]] control state on isolated mitochondria and tissue homogenate. The protocol can be applied to cells or tissue types tha

== Strengths and limitations == ...ell [[coupling-control protocol]] that provides information on [[ROUTINE]] and [[Electron transfer pathway]] in the presence of ([[MitoKit-CII/Succinate-n

...n mitochondrial preparations; mt: isolated mitochondria, tissue homogenate and permeabilized cells Communicated by [[Komlodi T]] and [[Gnaiger E]] (last update 2019-06-06)

...e., safety information, product use limitations and warranty information, and the Product Specification Sheet by Pressure BioSciences Inc (PBI): [[MiPNet ...Tube by unscrewing the Shredder-Screw Cap using the Shredder-Tube Cap Tool and removing the cap, for transfer of the sample into the [[Oroboros O2k|O2k-ch

|info='''A''' [[Coupling-control protocol]] and [[Crabtree effect]] with glycolysis inhibition- '''[[SUIT-003]]''' == Strengths and limitations ==

== Strengths and limitations == ...washing is required after the experiment to avoid carry-over of uncoupler and inhibitors, if used.

...'''C''' '''[[SUIT-003]]''' Second version of the Coupling-control protocol and respirometric cell viability test '''(CCV protocol)''' == Strengths and limitations ==

...ctionality of isolated mitochondria, additionally taking into account time and cost requirements of the procedure. ...ssue. It is pivotal that during the entire isolation procedure the samples and buffers are kept on ice.

...ernative oxidase]] pathway (Q-AOX), which is [[Antimycin_A| antimycin A]]- and [[cyanide| cyanide]] resistant. This protocol must be complemented with the == Strengths and limitations ==

...e]] pathway (CIII-CIV), which is inhibited by [[Antimycin_A| antimycin A]] and [[cyanide| cyanide]]. This protocol must be complemented with the study in == Strengths and limitations ==

== Strengths and limitations == ...absence of other, potentially interfering pathways, both in the LEAK state and in OXPHOS.

...ty| ''E'']]) with NADH-linked substrates ([[PM-pathway control state|PM]]) and the contribution of the [[S-pathway control state| S-pathway]] to the ET st == Strengths and limitations ==

== Strengths and limitations == :::# Malate concentration that does not stimulate malate anaplerotic pathway, and therefore could be used to study [[F-pathway]] without [[N-pathway]] contri

...mt-membrane potential (Safranin, TMRM), Ca²⁺ (Calcium Green™), and numerous other applications open for O2k-user innovation. ...ww.oroboros.at/index.php/product/o2k-fluo-smart-module/ '''Product details and purchase information''']

== Strengths and limitations == ...ol to assess just respiration since rotenone is not added at the beginning and does not prevent the inhibitory effect of oxaloacetate on the succinate deh

|info='''A: simultaneous determination of O2 flux and mt-membrane potential''' ...pathway control state| NS-pathway]] control state on isolated mitochondria and tissue homogenate. The protocol can be applied to cells or tissue types tha

...e sensitivity of the Amplex UltraRed® (AmR) changes over experimental time and upon addition of chemicals. To correct H₂O₂ flux for == Strengths and limitations ==

== Strengths and limitations == :::+ Pathway control in OXPHOS (F, F(N), FN, FNS and S pathways) can be evaluated.

''Ren'' may be higher than ''Rox''. Correspondingly, Q and NAD are not fully oxidized in the REN state compared to the ROX state. ...inguished from the [[ROX]] state. However, in novel applications (Q-Module and NADH-Module), a distinction of these states is necessary. Care must be take

|info='''A''' '''[[SUIT-003]]''' - [[Coupling-control protocol]] and [[Crabtree effect]] == Strengths and limitations ==

...otocol]] in the [[N-pathway]] for isolated mitochondria, tissue homogenate and cells permeabilized before addition to the O2k chamber. Communicated by [[Cardoso LHD]], [[Komlodi T]] and [[Gnaiger E]] (last update 2019-06-28)

|info='''A''' Coupling-control protocol and Crabtree effect test -'''[[SUIT-003]]''' complex cell [[coupling-control pr Communicated by [[Komlodi T ]], [[Garcia-Souza LF]], [[Cardoso LHD]] and [[Gnaiger E]] (last update 2019-04-03)

...s been designed to test the mitochondrial respiration at low oxygen levels and to modify the oxygen concentration in the same protocol. To decrease or inc == Strengths and limitations ==

...nd inorganic phosphate (which may not be the case in [[State 3]]), oxygen, and defined reduced CHNO-fuel substrates. ...te. 2[H] indicates the reduced hydrogen equivalents of CHO substrates (NS) and electron transfer to oxygen. H⁺_out are protons pumped

...] and [[FNS]] in [[Oxidative phosphorylation|OXPHOS state]] and of [[FNS]] and [[S]] in [[ET capacity| ET state]] is evaluated. ...ty in mitochondrial preparations (isolated mitochondria, tissue homogenate and permeabilized cells)

...tential (Safranin, TMRM, Rhodamine 123), Ca²⁺ (Calcium Green™), and numerous other applications open for O2k-user innovation. ...www.oroboros.at/index.php/product/o2k-fluo-led2-module/ '''Product details and purchase information''']

== Strengths and limitations == ...ell [[coupling-control protocol]] that provides information on [[ROUTINE]] and [[Electron transfer pathway]] in the presence of ([[MitoKit-CII/Succinate-n

...in mitochondrial preparations (isolated mitochondria, permeabilized cells, and tissue homogenate)-'''[[SUIT-021]]''' ...cin A dissipates mt-membrane potential. For mt-membrane potential analysis and calculation, visit this page: [[Mitochondrial membrane potential]].

== Strengths and limitations == ...r comparison of respiratory control in a large variety of species, tissues and cell types. Both SUIT protocols provide a mitochondrial mapping which allow

...T-018]]''' - SUIT protocol for simultaneous determination of O₂ and H₂O₂ flux in permeabilized cells at various oxygen co ...he AmR assay can be tested in separate protocols: [[SUIT-003 AmR ce D058]] and [[SUIT-003 AmR ce D059]]. For further information, ''see'': [[Amplex UltraR

== Strengths and limitations == ...r comparison of respiratory control in a large variety of species, tissues and cell types. Both SUIT protocols provide a mitochondrial mapping which allow

...nt in mitochondrial preparations (isolated mitochondria, tissue homogenate and permeabilized cells) ...cated by [[Cardoso LH]], [[Komlodi T]], [[Huete-Ortega M]], [[Doerrier C]] and [[Gnaiger E]] (last update 2019-06-06)

...-pathway state]] with glutamate and malate as substrates in the [[OXPHOS]] and [[Electron transfer pathway]] [[Coupling control state|coupling control sta Communicated by [[Cardoso LH]], [[Komlodi T]], [[Doerrier C]] and [[Gnaiger E]] (last update 2019-02-25)

...ntrol_state|F-pathway]] in [[LEAK respiration#The_LEAK_state |LEAK state]] and [[Oxidative phosphorylation|OXPHOS state]]''' ...] and [[FNS]] in [[Oxidative phosphorylation|OXPHOS state]] and of [[FNS]] and [[S]] in [[ET capacity| ET state]] can be evaluated. SUIT-015 can be extend

|info='''A''' '''[[SUIT-003]]''' - [[Coupling-control protocol]] and respirometric cell viability test; ce: living cells, pce: permeabilized cel == Strengths and limitations ==

...titration of digitonin, since the mitochondrial outer membrane is affected and [[cytochrome c|cytochrome ''c'']] can be released. Therefore, it is recomme == Strengths and limitations ==

...control of [[Fatty_acid_oxidation_pathway_control_state|F]], F(N), [[FN]] and [[FNS]] pathways in OXPHOS state can be evaluated by using this SUIT protoc ...ochondria|isolated mitochondria]], [[Tissue homogenate|tissue homogenate]] and [[Permeabilized cells|permeabilized cells]] (already permeabilized when the

...upling-control protocol to analyze mitochondrial O₂ consumption and the redox state of the Q-pool in S(Rot)-pathway control state in isolated m Communicated by [[Komlodi T]] and [[Cardoso LHD]] (last update 2023-12-18)

{{MitoPedia O2k and high-resolution respirometry}} == Strengths and limitations ==

...to substitute the more explicit expression for respiratory activity. ''R'' and growth of cells is supported by exogenous substrates in culture media. In m ...phate dehydrogenase complex (C_GpDH), amino acid oxidation (AAO) and fatty acid oxidation (FAO through the electron transferring flavoprotein co

== Strengths and limitations == ...ount (''N''_ce) is the sum of viable cells (''N''_vce) and dead cells (''N''_dce).

...parations]] (isolated mitochondria, tissue homogenate, permeabilized cells and permeabilized fibers). [[Malate]] alone does not support respiration of mt- Communicated by [[Cardoso LHD]], [[Garcia-Souza LF]] and [[Doerrier Carolina|Doerrier C]] (last update 2020-04-30)

...ce in mitochondrial preparations (isolated mitochondria, tissue homogenate and permeabilized cells)- '''[[SUIT-032]]''' == Strengths and limitations ==

...l with NADH-linked substrates for isolated mitochondria, tissue homogenate and permeabilized cells- '''[[SUIT-012]]''' == Strengths and limitations ==

Communicated by [[Cardoso LHD]], [[Doerrier C]] and [[Gnaiger E]] (last update 2019-03-06) == Strengths and limitations ==

== Steps and respiratory States == == Strengths and limitations ==

== Strengths and limitations == ...tial states by ROX measured in RP1-6 Ama may represent an over-correction, and inhibition takes a very long time in several cases ([[Pesta 2012 Methods Mo

...ce in mitochondrial preparations (isolated mitochondria, tissue homogenate and permeabilized cells)- '''[[SUIT-034]]''' == Strengths and limitations ==

{{MitoPedia O2k and high-resolution respirometry}} ...physiologically relevant estimate of maximum [[OXPHOS capacity| OXPHOS-]] and [[ET capacity |ET capacity]]. SUIT-005 can be extended with the CIV assay m

...hway control is studied in the [[Oxidative phosphorylation|OXPHOS state]], and [[FN]]—[[FNS]]—[[S]] in the [[ET capacity| ET state]]. === SUIT-001 O2 mt D001 and SUIT-001 O2 pfi D002 ===

|info='''B''': CCP(P) and cell membrane permeability test with succinate - [[SUIT-003]] ...[[MitoPedia: SUIT]]''' - [[coupling-control protocol]] (CCP) with pyruvate and cell membrane permeability test

...ce in mitochondrial preparations (isolated mitochondria, tissue homogenate and permeabilized cells)- '''[[SUIT-033]]''' == Strengths and limitations ==

...ce in mitochondrial preparations (isolated mitochondria, tissue homogenate and permeabilized cells)- '''[[SUIT-006]]''' == Strengths and limitations ==

Communicated by [[Doerrier C]] and [[Gnaiger E]] (last update 2020-09-03) == Strengths and limitations ==

...nd index of cardiorespiratory fitness, measured by spiroergometry on human and animal organisms capable of controlled physical exercise performance on a t ...he_XXX:_XXX-mass_Carol_on_BME_and_mitObesity |'''''XXX''-mass Carol on BME and mitObesity''']] of '''''X''-mass Carol'''

...upling control protocol to analyze mitochondrial O₂ consumption and the redox state of the Q-pool in S(Rot)-pathway control state in permeabili {{MitoPedia O2k and high-resolution respirometry}}

== Strengths and limitations == ...ntially interfering pathways, both in the [[LEAK respiration| LEAK state]] and in [[Oxidative phosphorylation| OXPHOS]].

== Strengths and limitations == ...ount (''N''_ce) is the sum of viable cells (''N''_vce) and dead cells (''N''_dce).

...respiration|''L''(Omy)]]. Tissue homogenates, permeabilized muscle fibers and permeabilized cells contain all cellular membranes with high ATPase activit == Strengths and limitations ==

...reduction of the dinucleotide cofactor NAD⁺ or NADP⁺ and a requirement for divalent cations (Mg²⁺ or Mn²⁺) as ...nt role in [[anaplerosis]] when glucose is limiting, particularly in heart and skeletal muscle. [[Tartronic acid]] (hydroxymalonic acid) is an inhibitor o

|info='''A: SUIT protocol for mt; mt: isolated mitochondria and tissue homogenate - '''[[SUIT-008]]''' Communicated by [[Doerrier C]] and [[Gnaiger E]] (last update 2019-06-06)

...[living cells]] with cell permeable compounds [[MitoKit-CII/Succinate-nv]] and [[MitoKit-CII/Malonate-nv]] - [[SUIT-003 O2 ce D060]] ...of the plasma membrane-permeable succinate ([[MitoKit-CII/Succinate-nv]]) and plasma membrane-permeable malonate ([[MitoKit-CII/Malonate-nv]]) in living

Communicated by [[Doerrier C]] and [[Gnaiger E]] (last update 2019-06-05) == Strengths and limitations ==

...is due to an obesogenic lifestyle with lack of physical exercise ('couch') and caloric surplus of food consumption ('potato'), causing several comorbiditi ...rint in preparation on [[body mass excess |'''BME''']] and [[:Category:BME and mitObesity |'''mitObesity''']].

...P) with plasma membrane-permeable succinate ([[MitoKit-CII/Succinate-nv]]) and plasma membrane-permeable malonate ([[MitoKit-CII/Malonate-nv]]) in living Communicated by [[Garcia-Souza LF]], [[Cardoso LHD]] and [[Gnaiger E]] (last update 2019-09-24)

== Strengths and limitations == :::+ The presence of PGM and S establishes fully operative TCA cycle activity.

...ide spectrum of degenerative diseases. It is an inhibitor of [[Complex I]] and [[glycerophosphate dehydrogenase complex]]. == Metformin and mitObesity ==

Communicated by [[Cardoso LHD]] and [[Gnaiger E]] (last update 2019-02-14) == Strengths and limitations ==

...lammatory, anti-oxidant, anti-carcinogenic, anti-mutagenic, anti-coagulant and anti-infective effects. The protective effects of curcumin on rat heart mit == Curcumin and mitObesity ==

...ntrol_state|F-pathway]] in [[LEAK respiration#The_LEAK_state |LEAK state]] and [[Oxidative phosphorylation|OXPHOS state]]''' ...OS state]] and of [[S]] in [[LEAK respiration#The_LEAK_state |LEAK state]] and [[ET capacity| ET state]] is evaluated.

|info='''A''' to analyse O2 flux and the Q-redox state in the N-,NS- and S- pathway control state on isolated mitochondria- '''[[SUIT-031]]''' == Strengths and limitations ==

...13 Tissue and cell: Skeletal muscle|Skeletal muscle]] ••• [[MiP2013 Tissue and cell: Nervous system|Brain;Nervous system]]''' ...13 Stress: Hypoxia|Hypoxia]]''' - High-altitute mitochondrial physiology and hypoxia (supported by COST project HypoxiaNet for members of [http://www.hy

...;2c;3U;4G;5S;(6Oct);7Rot;8Gp;9Ama;10AsTm;11Azd.png|400px|SUIT-RP1 for PBMC and PLT]] ...rence protocol RP1 for living PBMC and PLT cells (ce, ROUTINE respiration) and cells that are permeabilized in the chamber (pce)'''

|info='''A''' to analyse O2 flux and the Q-redox state in the NS- pathway control state on permeabilized cells- == Strengths and limitations ==

...iency is independent of kinetic control by the ''E-P'' control efficiency, and is equal to the [[P-L control efficiency |''P-L'' control efficiency]] if ' ...oxia |»Bioblast link]]«</ref>,<ref>Gnaiger E (2015) Cell ergometry: OXPHOS and E-L coupling efficiency. Mitochondr Physiol Network 2015-01-18. [[OXPHOS_co

:::: '''Find the WG summaries in the Minutes and also below''' ...rial physiological research as a milestone towards unification of concepts and nomenclature.

...ocol]] Reference protocol RP1 for isolated mitochondria, tissue homogenate and permeabilized cells''' == Strengths and limitations ==

...ux in mitochondrial preparations (isolated mitochondria, tissue homogenate and permeabilized cells)-'''[[SUIT-018]]''' ...t the same state for high (tissue hyperoxia; [O₂]> ~100-110 µM) and low (tissue normoxia; [O₂]< ~70-80 µM) oxygen concentration, or

== Strengths and limitations == ...superior alternative to GM: with GM the fraction of the N-pathway is lower and S-pathway contribution is higher compared to PM. PM, therefore, yields a mo

...otocol]] Reference protocol RP1 for living cells (ce, ROUTINE respiration) and cells permeabilized in the respirometric chamber (pce)''' Communicated by [[Doerrier C]] and [[Gnaiger E]] (last update 2022-07-07)

...ocol]] Reference protocol RP2 for isolated mitochondria, tissue homogenate and permeabilized cells''' Communicated by [[Doerrier C]] and [[Gnaiger E]] (last update 2019-09-24)

...b> in mitochondrial preparations (isolated mitochondria, tissue homogenate and permeabilized cells)- '''[[SUIT-006]]''' ...eabilized when they are added to the chamber) in a wide variety of species and tissues. After addition of the NADH-linked substrates, the addition of ADP

...ity| ''E'']]) with NADH-linked substrates ([[PM-pathway control state|PM]] and PGM)''' ...ity| ''E'']]) with NADH-linked substrates ([[PM-pathway control state|PM]] and [[PGM]]). Addition of [[Glutamate|G]] enables evaluating the contribution o

...c;4M2;5P;6G;7S;8Gp;9U;10Rot;11Ama;12AsTm;13Azd.png|400px|SUIT-RP2 for PBMC and PLT]] ...e protocol RP2 for for living PBMC and PLT cells (ce, ROUTINE respiration) and cells that are permeabilized in the chamber (pce)'''

...otocol]] Reference protocol RP2 for living cells (ce, ROUTINE respiration) and cells that are permeabilized in the chamber (pce)''' Communicated by [[Doerrier C]] and [[Gnaiger E]] (last update 2019-09-24)

...weight (BMI<18.5 kg·m^-2), overweight (BMI>25 kg·m^-2) and obesity (BMI>30 kg·m^-2). Keys et al (1972; see 2014) emphasized ...he_XXX:_XXX-mass_Carol_on_BME_and_mitObesity |'''''XXX''-mass Carol on BME and mitObesity''']] of '''A ''X''-mass Carol'''

== Strengths and limitations == ...ount (''N''_ce) is the sum of viable cells (''N''_vce) and dead cells (''N''_dce).

== Strengths and limitations == :::+ The presence of PGM and S establishes fully operative TCA cycle activity.

== Strengths and limitations == :::+ The presence of PGM and S establishes fully operative TCA cycle activity.

|info='''A: A protocol for oxygen dependence of O₂ flux and H₂O₂ flux in isolated mitochondria,tissue homogenate ...to evaluate H₂O₂ flux in the NS-pathway in the LEAK and OXPHOS states. To decrease or increse the oxygen concentration in the O2k-C

...ways which are most important in the mitochondria of many species, tissues and cell types. == Strengths and limitations ==

...on in mitochondrial preparations (isolated mitochondria, tissue homogenate and permeabilized cells) -'''[[SUIT-006]]''' == Strengths and limitations ==

|info='''A: Additivity between the N- and [[Succinate pathway control state| S-pathway]] in the Q-junction''' ...s as a diagnostic tool for the activity of the [[glutamate dehydrogenase]] and its linked pathways, which could be relevant in some pathologies. SUIT-008

...ng-control protocol]] for simultaneous determination of O₂ flux and mitochondrial ATP production in permeabilized cells -'''[[SUIT-006]]''' == Strengths and limitations ==

...f the S-pathway may be higher with PGM. ''Compare'' [[1PM;2D;3U;4S;5Rot-]] and [[SUIT-001|1PM;2D;3U;4G;5S;6Oct;7Rot;8Gp- ]]. ...ing state. With N_''P'', NS_''P'', NS_''E'', and S_''E''. If independent information is available on S<sub>''P''<

...''Rox''-corrected rates, in contrast to total rates ''R''´, ''L''´, ''P''´ and ''E''´. When expressing ''Rox'' as a fraction of ET capacity ([[flux contr ...distinguished from the ROX state. However, in novel applications (Q-Module and NADH-Module), a distinction of these states is necessary. Care must be take

...': '''[[IOC101|101st OROBOROS O2k-Workshop on high-resolution respirometry and O2k-Fluorometry]]''', integrated in the 8th MiP''school'', London 2015 == [http://mitochondria.cs.ucl.ac.uk/mip2015/?page_id=30 Registration and Abstracts] ==

...H₂O₂ production) changes over the experimental time and upon addition of chemicals. To correct H₂O₂ flux for ...[[SUIT-009 O2 mt D015]] or [[SUIT-009 O2 ce-pce D016]]; for O₂ and H₂O₂ measurements (AmR), apply [[SUIT-009 AmR mt D021

{{MitoPedia O2k and high-resolution respirometry}} ...thway control state| NS]], [[FNS]], [[Succinate pathway control state| S]] and [[SGp-pathway control state| SGp]] pathways) can be evaluated by using this

Communicated by [[Doerrier C]] and [[Gnaiger E]] (last update 2019-03-06) == Strengths and limitations ==

..., [[FNS]], [[FNSGp]] pathways) and in [[ET capacity| ET state]] ([[FNSGp]] and [[SGp-pathway control state|SGp]]) can be evaluated by using this SUIT prot Communicated by [[Doerrier C]] and [[Gnaiger E]] (last update 2019-06-05)

...rogenase Complex]] oxidizes glycerophosphate to dihydroxyacetone phosphate and feeds electrons directly to ubiquinone. ::::# Weigh 324 mg of Gp and dissolve in 845 µL H2O.

...ion between electric force expressed in joules per coulomb or Volt [V=J/C] and chemical force with the unit joules per mole or Jol [Jol=J/mol], ...g the ion reporter [[TPP+]]. All mentioned ion indicator molecules inhibit and may uncouple respiration, which makes it essential to test the optimum conc

...phosphorylation|OXPHOS]] with [[NS-pathway control state|NS substrates]]) and coupling/pathway control''' ...-pathway control state|NS]]) and ET state ([[NS-pathway control state|NS]] and [[Succinate pathway control state|S]]). SUIT-011 can be extended with the C

...ow]], ''I''_O₂''L''). If these conditions are defined and remain consistent within a given context, then the simple symbol ''L'' for ...on is possible in living cells by inhibition of the phosphorylation system and in mt-preparations supported by an ET-pathway competent substrate state, ex

...Biol |»Bioblast link«]]</ref>,<ref>Gnaiger E (2020) Mitochondrial pathways and respiratory control. An introduction to OXPHOS analysis. 5th ed. Bioenerg C ...eparations) state of respiration, mitochondria are both, partially coupled and partially uncoupled. The uncoupled part of OXPHOS capacity is smaller than

...approach and can be easily used in combination with the permeability test and/or the assessment of the complex IV capacity. * [[SUIT-003 O2 ce-pce D020]] coupling-control protocol and respirometric cell viability test (CCV protocol).

...oupled states controlled by intrinsic uncoupling (e.g. UCP1 in brown fat), and (''3'') pathological dyscoupled states indicative of mitochondrial injuries ...urement of ET capacity is possible by uncoupler titrations in living cells and in mt-preparations supported by an ET-pathway competent state, exemplified

...iology'''. Establishing a comprehensive database will require global input and cooperation. ...evaluation of altered mitochondrial respiratory control patterns in health and disease'' ([[Gnaiger 2009 Int J Biochem Cell Biol|Gnaiger 2009]]).

...(n)]]. Therefore, [[oligomycin]] can be used to verify whether this occurs and obtain the [[LEAK respiration|LEAK]] state appropriately. Since higher conc ...membrane potential measurements, choose [[SUIT-006 Fluo mt D034]]. For O2 and Q redox state measurements, choose [[SUIT-006 Q mt D071]] or [[SUIT-006 Q c

...RCR equals ''P/L''. Under these conditions, the respiratory control ratio and ''P-L'' control efficiency are related by a hyperbolic function, ''j<sub>P- » [[#Cell ergometry: OXPHOS-control and ET-coupling efficiency |'''MiPNet article''']]

...mitochondrial respiratory studies with peripheral blood mononuclear cells and platelets.''' - Updated: 2020-03-06 - *''Confirmed'' ...ical phases of blood cell preparation and respiration in living cells (ce) and permeabilized cells (pce).]]

...ectrum of action particularly at the level of mitochondria. » [[#Melatonin and protection from mitochondrial damage |'''MiPNet article''']] = Melatonin and protection from mitochondrial damage =

...2017 Mar 21-23. Mitochondrial fitness mapping: Conference, Working Groups and MC meeting - [[COST Action MitoEAGLE]]. :::: MitoEAGLE Conference, Working Groups and Management Committee Meeting

...''', although several studies point towards a link between viral infection and mitochondrial dysfunction using high-resolution respirometry, with potentia == Obesity and COVID-19 severity ==

...A balanced BME is BME° = 0.0 with a band width of -0.1 towards underweight and +0.2 towards overweight. The BME is linearly related to the [[body fat exce ...he_XXX:_XXX-mass_Carol_on_BME_and_mitObesity |'''''XXX''-mass Carol on BME and mitObesity''']] of '''A ''X''-mass Carol'''

...rgl AT''', 2017 Jul 23-30. '''10^th MiP''school'' 2017 MitoEAGLE and MitoEAGLE Workshop WG1-4. ...https://www.uibk.ac.at/forschung/doktoratskollegs/index.html.de| DK Ageing and Regeneration]]

...or mitochondrial preparations. The reaction of H₂O₂ and AmR is catalyzed by [[horseradish peroxidase]] to produce the red fluoresce |info=Komlódi T, Sobotka O, Gnaiger E (2021) Facts and artefacts on the oxygen dependence of hydrogen peroxide production using Am

'''Diagnosis of mitochondrial functions and mitochondrial injuries''' ...e diseases. MitoFit is a gateway and milestone to better diagnose, support and treat patients in our modern, rapidly aging society.