O2k-Demo experiments

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O2k-Demo experiments

O2k-Procedures
SUIT-Protocol

Oroboros O2k demo

ProtocolViewTopicPreparationTissue and cellMammal and modelLast update
MiPNet12.23 FiberRespirationBioblast pdf »Versions
O2k-Protocols contents
Mitochondrial respiration in permeabilized fibers: Needle biopsies from horse skeletal muscle.
Permeabilized tissueSkeletal muscleHorse2019-01-10
MiPNet12.01 Suppl T-issueBioblast pdf »Versions
O2k-Protocols contents
MitoPathways at the Q-junction: mouse skeletal muscle fibers.
Permeabilized tissueSkeletal muscleMouse2018-11-18
MiPNet15.09. Yeast: HRR Reference AssayBioblast pdf  »Versions
O2k-Protocols
A mitochondrial reference assay for high-resolution respirometry using freeze-dried Baker’s yeast.
Saccharomyces cerevisiae
Fungi
2017-02-05
MiPNet08.09 CellRespirationBioblast pdf  »Versions
O2k-Procedures contents
High-resolution respirometry and coupling control protocol with living cells: ROUTINE, LEAK, ET-pathway, ROX.
Intact cellsBlood cells
Lymphocyte
Human2016-08-11
MiPNet10.04 CellRespirationBioblast pdf  »Versions
O2k-Protocols
An experiment with high-resolution respirometry: coupling control in cell respiration.
Intact cellsBlood cellsMouse2016-08-08
MiPNet17.03 Shredder vs FibersBioblast pdf »Versions
O2k-Protocols
Mitochondrial respiration in permeabilized fibers versus homogenate from fish liver and heart. An application study with the PBI-Shredder.
HomogenateHeart
Liver
Fishes2015-06-18
MiPNet12.11 MitoRespirationBioblast pdf »Versions
O2k-Protocols
Flux control ratios in isolated mitochondria. OXPHOS capacity and respiratory control.
Isolated mitochondriaArtemia
Crustaceans
2014-02-20
Gnaiger 2014 MitoPathwaysBioblast pdf
O2k-Protocols
Gnaiger E (2014) Mitochondrial pathways and respiratory control. An introduction to OXPHOS analysis. 4th ed. Mitochondr Physiol Network 19.12. Oroboros MiPNet Publications, Innsbruck:80 pp.
Intact cells
Permeabilized cells
Permeabilized tissue
Homogenate
Isolated mitochondria
Heart
Skeletal muscle
Fibroblast
Human
Mouse
2014
Pesta 2012 Methods Mol BiolBioblast pdf
PMID: 22057559
O2k-Protocols
Pesta D, Gnaiger E (2012) High-resolution respirometry. OXPHOS protocols for human cells and permeabilized fibres from small biopsies of human muscle. Methods Mol Biol 810:25-58.
Intact cells
Permeabilized cells
Permeabilized tissue
Fibroblast
HEK
HUVEC
Other cell lines
Skeletal muscle
Human2012
MiPNet12.17 BovineHeartMitoBioblast pdf »Versions
O2k-Protocols contents
Mitochondrial respiration with frozen bovine heart mitochondria: diagnostic tests.
SMPHeartBovines2011-12-11
TIP2k-Module

O2k-Demo TIP2k

 » O2k-Catalogue: TIP2k
 » O2k-Publications: TIP2k
ProtocolViewTopicPreparationTissue and cellMammal and modelLast update
MiPNet10.04 CellRespirationBioblast pdf  »Versions
O2k-Protocols
An experiment with high-resolution respirometry: coupling control in cell respiration.
Intact cellsBlood cellsMouse2016-08-08
Pesta 2012 Methods Mol BiolBioblast pdf
PMID: 22057559
O2k-Protocols
Pesta D, Gnaiger E (2012) High-resolution respirometry. OXPHOS protocols for human cells and permeabilized fibres from small biopsies of human muscle. Methods Mol Biol 810:25-58.
Intact cells
Permeabilized cells
Permeabilized tissue
Fibroblast
HEK
HUVEC
Other cell lines
Skeletal muscle
Human2012
MiPNet04.05 Titration-InjectionBioblast pdf
O2k-Protocols contents
From step titration to ramp injection: Uncoupling by FCCP with TIP.
Intact cellsEndothelial;epithelial;mesothelial cell
HUVEC
Human1999-01

TIP2k Manual

ChapterViewSectionLast update
MiPNet12.10 TIP2k-manualBioblast pdf »Versions
O2k-Manual
Titration-Injection microPump. TIP2k user manual.
2016-08-10



O2k-FluoRespirometer
link=O2k-TPP%2B_and_Ca2%2B_ISE-Module

O2k-Demo O2k-MultiSensor

 » O2k-Catalogue: O2k-MultiSensor
 » O2k-Publications: O2k-Fluorometry
ProtocolViewTopicPreparationTissue and cellMammal and modelLast update
MiPNet20.14 AmplexRed H2O2-productionBioblast pdf »Versions
O2k-Protocols
O2k-FluoRespirometry: HRR and simultaneous determination of H2O2 production with Amplex UltraRed.
Isolated mitochondriaHeartMouse2018-11-26
MiPNet18.06 Amplex-YeastBioblast pdf »Versions
O2k-Protocols
O2k-FluoRespirometry: Amplex UltraRed using freeze-dried baker’s yeast.
Intact cellsSaccharomyces cerevisiae
Fungi
2018-11-25
MiPNet20.13 Safranin mt-membranepotentialBioblast pdf »Versions
O2k-Protocols
O2k-FluoRespirometry: HRR and simultaneous determination of mt-membrane potential with safranin or TMRM.
HomogenateNervous systemMouse2018-09-25
MiPNet14.05 TPP-mtMembranePotentialBioblast pdf »Versions
O2k-Protocols contents
TPP+ and mt-membrane potential.
Homogenate
Isolated mitochondria
Nervous systemMouse2016-04-01
MiPNet17.16 O2k-SpectrophotometryBioblast pdf »Versions
O2k-Protocols
O2k-Spectrophotometry - A MitoCom Project.
2015-06-16
MiPNet18.05 Amplex-Mouse-heartBioblast pdf »Versions
O2k-Protocols
O2k-Fluorometry: HRR and H2O2 production in mouse cardiac tissue homogenate.
HomogenateHeartMouse2015-06-16
Krumschnabel 2015 Methods Mol BiolPMID: 25631019
O2k-Protocols contents
Krumschnabel G, Fontana-Ayoub M, Sumbalova Z, Heidler J, Gauper K, Fasching M, Gnaiger E (2015) Simultaneous high-resolution measurement of mitochondrial respiration and hydrogen peroxide production. Methods Mol Biol 1264:245-61.
Isolated mitochondriaNervous systemMouse2015
Makrecka-Kuka 2015 BiomoleculesPMID: 26131977 Open Access
O2k-Protocols contents
Makrecka-Kuka M, Krumschnabel G, Gnaiger E (2015) High-resolution respirometry for simultaneous measurement of oxygen and hydrogen peroxide fluxes in permeabilized cells, tissue homogenate and isolated mitochondria. Biomolecules 5:1319-38.
Intact cells
Permeabilized cells
Homogenate
Isolated mitochondria
Heart
Nervous system
HEK
Human
Mouse
2015
Krumschnabel 2014 Methods EnzymolPMID: 24862266 »O2k-brief
O2k-Protocols contents
Krumschnabel G, Eigentler A, Fasching M, Gnaiger E (2014) Use of safranin for the assessment of mitochondrial membrane potential by high-resolution respirometry and fluorometry. Methods Enzymol 542:163-81.
HomogenateNervous systemMouse2014
MiPNet15.08 TPP electrodeBioblast pdf »Versions
O2k-Protocols
Ion selective electrode for TPP+ and high-resolution respirometry.
2011-12-11
MiPNet08.12 IOC22Bioblast pdf
O2k-Protocols
NO effect on mitochondrial oxygen kinetics at low oxygen. O2k workshop Report.
Isolated mitochondriaLiverRat2011-12-11

O2k-MultiSensor Manual

ChapterViewSectionLast update
MiPNet15.03 O2k-MultiSensor-ISEBioblast pdf »Versions
O2k-Manual
O2k-MultiSensor system with ion selective electrodes (ISE).
2018-01-25
MiPNet15.05 NO-manualBioblast pdf  »Versions
O2k-Manual
O2k-MultiSensor system with amperometric sensors: NO, H2S, H2O2.
2016-04-21
MiPNet17.05 O2k-Fluo LED2-ModuleBioblast pdf »Versions
O2k-Manual
O2k-Fluo LED2-Module.
2016-08-09
MiPNet23.15 O2k-pH ISE-Module
O2k-Manual
Measurement of proton flux with the O2k-pH ISE-Module.
2018-09-08


O2k-Guides: »O2k-Catalogue :: »O2k-Manual :: »O2k-Publications

O2k-Procedures » Link
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» O2k-Procedures: MitoPathways
SUIT-Protocol
» O2k-Demo experiments
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» O2k-chemicals and media
PBI-Shredder HRR-Set.JPG
» O2k-mitochondrial preparations
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» O2k-Procedures: Mitochondrial and marker-enzymes