Reference: A Determination of the respiration due to the cytochrome c oxidase pathway -SUIT-023
SUIT number: D053_ce1;ce2SHAM;ce3KCN
The SUIT-023 O2 ce D053 has been designed to provide an estimation of the mitochondrial respiration in microalgal intact cells due to the cytochrome c oxidase pathway, which is non-resistant to cyanide-and antimycin A inhibition. This protocol must be complemented with the study in parallel of the mitochondrial respiration due to the alternative oxidase pathway (see SUIT-022).
Communicated by Huete-Ortega M and Gnaiger E (last update 2019-04-09)
Steps and respiratory states
|Step||State||Pathway||Q-junction||Comment - Events (E) and Marks (M)|
|ce2SHAM||ROUTINE OR ROX|| ce1;ce2SHAM
- » Selected keywords in SUIT protocols
- Pathway control
Strengths and limitations
- + Short duration of experiment
- - Estimation of the linear coupling control is not done
- - The use of cyanide requires a careful and in deep cleaning of the chambers after use and careful handling of the chemical to guarantee health safety.
Compare SUIT protocols
MitoPedia methods: Respirometry