MitoPedia: SUIT A

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MitoPedia

MitoPedia: SUIT A

MitoPedia - high-resolution terminology - matching measurements at high-resolution.
The MitoPedia terminology is developed continuously in the spirit of Gentle Science.

MitoFit
In the Library of SUIT protocols
» MitoPedia: SUIT - SUIT concept and a list of all SUIT protocols in the library of SUIT protocols.
»A: MitoPedia: SUIT A - you are here: these are SUIT protocols used for cohort studies.
»B: MitoPedia: SUIT B - these are published SUIT protocols considered less suitable for cohort studies.
»C: MitoPedia: SUIT C - these are SUIT protocols used for exploratory research.
See also:
» O2k-Protocols: O2k-Demo experiments



Library of SUIT protocols: SUIT A

SUIT codeSUIT numberSUIT categoryCoupling/pathway control diagramO2k-ApplicationReference
SUIT-001 O2 ce-pce D003D003_ce1;1Dig;1PM;2D;2c;3U;4G;5S;6Oct;7Rot;8Gp;9Ama;10AsTm;11AzdFNSGp(Oct,PGM)SUIT-RP1O2A Reference protocol RP1; ce: non-permeabilized cells, pce: permeabilized cells - SUIT-001
SUIT-001 O2 ce-pce D004D004_ce1;1Dig;1PM;2D;2c;3U;4G;5S;(6Oct);7Rot;8Gp;9Ama;10AsTm;11AzdNSGp(PGM)SUIT-RP1 for PBMCs and PLTsO2A Reference protocol RP1 specific for PBMC and PLT cells; ce: non-permeabilized cells, pce: permeabilized cells - SUIT-001
SUIT-001 O2 mt D001D001_1PM;2D;2c;3U;4G;5S;6Oct;7Rot;8Gp;9Ama;10AsTm;11AzdFNSGp(Oct,PGM)SUIT-RP1O2A Reference protocol RP1; mt: isolated mitochondria, tissue homogenate and permeabilized cells - SUIT-001
SUIT-001 O2 pfi D002D002_1PM;2D;2c;3U;4G;5S;6Oct;7Rot;8Gp;9Ama;10AsTm;11AzdFNSGp(Oct,PGM)SUIT-RP1O2A Reference protocol RP1; pfi: permeabilized fibers - SUIT-001
SUIT-002 O2 ce-pce D007D007_ce1;1Dig;1D;2M;3Oct;3c;4M;5P;6G;7S;8Gp;9U;10Rot;11Ama;12AsTm;13AzdFNSGp(Oct,PGM)SUIT-RP2O2A Reference protocol RP2; ce: non-permeabilized cells, pce: permeabilized cells - SUIT-002
SUIT-002 O2 ce-pce D007aD007a_ce1;1Dig;1D;2M;3Oct;3c;4M;5P;6G;7S;8Gp;9U;10Rot;11Ama;12AsTm;13AzdFNSGp(Oct,PGM)SUIT-RP2 for PBMCs and PLTsO2A Reference protocol RP2; pce: specific PBMC and PTL cells; ce: non-permeabilized cells, pce: permeabilized cells - SUIT-002
SUIT-002 O2 mt D005D005_1D;2M;3Oct;3c;4M;5P;6G;7S;8Gp;9U;10Rot;11Ama;12AsTm;13AzdFNSGp(Oct,PGM)1D;2M.1;3Oct;3c;4M2;5P;6G;7S;8Gp;9U;10Rot;11Ama;12AsTm;13Azd.pngO2A Reference protocol RP2; mt: isolated mitochondria, tissue homogenate and permeabilized cells - SUIT-002
SUIT-002 O2 pfi D006D006_1D;2M;3Oct;3c;4M;5P;6G;7S;8Gp;9U;10Rot;11Ama;12AsTm;13AzdFNSGp(Oct,PGM)SUIT-RP2O2A Reference protocol RP2; pfi: permeabilized fibers - SUIT-002
SUIT-003 O2 ce D009D009_ce1;ce2Omy;ce3U;ce4Rot;ce5AmaceCe1;ce2Omy;ce3U-.pngO2A: Coupling control in intact cells with oligomycin; ce: non-permeabilized cells -SUIT-003
SUIT-003 O2 ce D037D037_ce1;ce1Glc;(ce2Omy);ce3U;ce4AmaceGlcCe1;ce1Glc;ce2(Omy);ce3U;ce4Ama.pngO2A Coupling control protocol and Crabtree effect test -SUIT-003
SUIT-003 O2 ce D038D038_ce1;(ce2Omy);ce3U;ce3Glc;ce3'U;ce4AmaceGlcCe1;ce2(Omy);ce3U;ce3Glc;ce3'U;ce4Ama.pngO2A Coupling control protocol and Crabtree effect test -SUIT-003
SUIT-003 O2 ce D039D039_ce1;(ce2Omy);ce3U;ce4Rot;ce5AmaceCe1;(ce2Omy);ce3U;ce4Rot;ce5Ama.jpgO2A: Coupling control in intact cells without oligomycin; ce: non-permeabilized cells -SUIT-003
SUIT-003 O2 ce-pce D020D020_ce1;ce1P;ce2Omy;ce3U;ce4Rot;ce5S;1Dig;1c;2Ama;3AsTm;4AzdceP,SCe1;ce1P;ce2Omy;ce3U;ce4Rot;ce5S;1Dig;1c;2Ama;3AsTm;4Azd.pngO2A Coupling control protocol and respirometric cell viability test (CCV protocol); ce: non-permeabilized cells, pce: permeabilized cells - SUIT-003
SUIT-004 O2 pfi D010D010_1PM;2D;2c;3U;4S;5Rot;6Ama;7AsTm;8AzdNS(PM)1PM;2D;2c;3U;4S;5Rot;6Ama;7AsTm;8Azd.pngO2A: Short protocol linked to SUIT-001 O2 pfi D002 - SUIT RP1 (human skeletal muscle)-SUIT-004
SUIT-005 O2 pfi D011D011_1OctM;2D;3P;4S;5U;6Rot;7Ama;8AsTm;9AzdFNS(Oct,PM)1OctM;2D;2c;3P;4S;5U;6Rot;7Ama;8AsTm;9Azd.pngO2A: short protocol linked to SUIT-002 O2 pfi D006 - SUIT RP2 (human skeletal muscle) - SUIT-005
SUIT-006 AmR mt D048D048_1PM;2D;3Omy;4U;5AmaN(PM)1PM;2D;3Omy;4U;5Ama.pngAmRA: short protocol for simultaneous determination of O2 flux and H2O2 in mitochondrial preparations (isolated mitochondria, tissue homogenate and permeabilized cells)- SUIT-006
SUIT-006 Fluo mt D034D034_1PM;2D;3Omy;4U;5AmaN(PM)1PM;2D;3Omy;4U;5Ama.pngFluoA: short protocol for simultaneous determination of O2 flux and mitochondrial membrane potential in mitochondrial preparations (isolated mitochondria, tissue homogenate and permeabilized cells) -SUIT-006
SUIT-006 MgG mt D055D055_1PM;2D;3Cat;4U;5AmaN(PM)1PM;2D;3Cat;4U;5Ama.pngMgGA: short protocol for simultaneous determination of O2 flux and mitochondrial ATP production in mitochondrial preparations (isolated mitochondria, tissue homogenate and permeabilized cells) -SUIT-006
SUIT-006 O2 ce-pce D029D029_ce1;1Dig;1PM;2D;2c;3Omy;4U;5AmaN(PM)Ce1;1Dig;1PM;2D;2c;3Omy;4U;5Ama.pngO2A to analyse the coupling control on permeabilized cells in N(PM) pathway control state- SUIT-006
SUIT-006 O2 mt D022D022_1SRot;2D;2c;(3Omy);4U;5AmaS1SRot;2D;2c;3(Omy);4U;5Ama.pngO2A: Protocol for coupling control assessment in mitochondrial preparations (isolated mitochondria, tissue homogenate and permeabilized cells) SUIT-006
SUIT-006 O2 mt D047D047_1PM;2D;2c;3Omy;4U;5AmaN(PM)1PM;2D;2c;3Omy;4U;5Ama.pngO2A is to analyse the coupling control in NADH-pathway in mitochondrial prepartions (isolated mitochondria and tissue homogenate) - SUIT-006
SUIT-007 O2 ce-pce D030D030_ce1;1Dig;1G;2D;2c;3M;4U;5AmaN(GM)Ce1;1Dig;1G;2D;2c;3M;4U;5Ama.pngO2A pce: permeabilized cells - SUIT-007
SUIT-008 O2 ce-pce D025D025_ce1;1Dig;1PM;2D;2c;3G;4S;5U;6Rot;7Ama;8AsTm;9AzdNS(PGM)Ce1;1Dig;1PM;2D;2c;3G;4S;5U;6Rot;7Ama;8AsTm;9Azd.pngO2A: for cells-permeabilized cells Lemieux 2017 Sci Rep - SUIT-008
SUIT-008 O2 mt D026D026_1PM;2D;2c;3G;4S;4D;5U;6Rot;7Ama;8AsTm;9AzdNS(PGM)1PM;2D;2c;3G;4S;5U;6Rot;7Ama;8AsTm;9Azd.pngO2A: SUIT protocol for mt; mt: isolated mitochondria and tissue homogenate - SUIT-008
SUIT-008 O2 pfi D014D014_1PM;2D;2c;3G;4S;4D;5U;6Rot;7Ama;8AsTm;9AzdNS(PGM)1PM;2D;2c;3G;4S;5U;6Rot;7Ama;8AsTm;9Azd.pngO2A: O2 pfi D014 protocol was used a a reference protocol in MitoEAGLE project (WG2) to evaluate the researcher skills preparing permeabilized fibers (See Lemieux 2017 Sci Rep)- SUIT-008
SUIT-009 AmR ce-pce D019D019_ce1;1Dig;1S;2D;3P;4Rot;5AmaNS(P)SUIT9A: short protocol for H2O2 (AmR) in ce: non-permeabilized cells, pce: permeabilized cells -SUIT-009
SUIT-009 AmR mt D021D021_1S;2D;3P;4Rot;5AmaNS(P)SUIT-009AmRA: short protocol for H2O2 (AmR) in mitochondrial preparations; mt: isolated mitochondria, tissue homogenate and permeabilized cells - SUIT-009
SUIT-010 O2 ce-pce D008D008_ce1;ce2Rot;ce3S;ce4D;1Dig;1cceS,SRespirometric test of optimum digitonin concentrationO2A Optimum digitonin concentration for pce -SUIT-010
SUIT-010 O2 ce-pce D050D050_ce1;ce2Rot;ce3S(p);ce3S;ce4D;ce4c;1Dig;2AmaceS,SCe1;ce2Rot;ce3S(p);ce3S;ce4D;ce4c;1Dig;2Ama.pngO2A Evaluation of plasma membrane integrity of intact cells -SUIT-010
SUIT-011 O2 pfi D024D024_1GM;2D;2c;3S;4U;5Rot;6AmaNS(GM)1GM;2D;2c;3S;4U;5Rot;6Ama.pngO2A pfi: permeabilized fibers - SUIT-011
SUIT-012 O2 ce-pce D052D052_ce1;1Dig;1PM;2D;2c;3G;4U;5AmaN(PGM)Ce1;1Dig;1PM;2D;2c;3G;4U;5Ama.pngO2A Linear coupling control with NADH-linked substrates for pce - SUIT-012
SUIT-012 O2 mt D027D027_1PM;2D;2c;3G;4U;5AmaN(PGM)1PM;2D;3G;4U;5Ama.pngO2A Linear coupling control with NADH-linked substrates for isolated mitochondria, tissue homogenate and permeabilized cells- SUIT-012
SUIT-013 AmR ce D023D023_ce1ceSUIT013 AmR ce D023.pngAmRA SUIT-013
SUIT-014 O2 pfi D042D042_1GM;2D;3P;4S;5U;6Rot;7AmaNS(PGM)1GM;2D;2c;3P;4S;5U;6Rot;7Ama.pngO2A SUIT-014
SUIT-015 O2 pti D043D043_1OctM;2D;3G;4P;5S;6U;7Rot;8AmaFNS(Oct,PGM)1OctM;2D;3G;4P;5S;6U;7Rot;8Ama.pngO2A pti: permeabilized tissue- SUIT-015
SUIT-016 O2 pfi D044D044_1OctM;2D;3G;4S;5Rot;6Omy;7U;8AmaFNS(Oct,GM)1OctM;2D;3G;4S;5Rot;6Omy;7U;7c;8Ama.pngO2A pfi: permeabilized fibers- SUIT-016
SUIT-017 O2 mt D046D046_1OctM;2D;3G;3c;4S;5U;6Rot;7AmaFNS(Oct,GM)1OctM;2D;2c;3G;4S;5U;6Rot;7Ama.pngO2A: SUIT-017
SUIT-017 O2 pfi D049D049_1OctM;2D;2c;3G;4S;5U;6Rot;7AmaFNS(Oct,GM)1OctM;2D;2c;3G;4S;5U;6Rot;7Ama.pngO2A: SUIT-017
SUIT-018 AmR mt D031D031_1GMS;2D;3AmaNS(GM)1GMS;2D;3Ama.pngAmRA: for simultaneous determination of O2 and H2O2 flux in mitochondrial preparations (isolated mitochondria, tissue homogenate and permeabilized cells) at low oxygen concentration (tissue normoxia)-SUIT-018
SUIT-018 AmR mt D040D040_1GMS;2D;3AmaNS(GM)SUIT-018AmRA: simultaneous determination of O2 and H2O2 flux in mitochondrial preparations (isolated mitochondria, tissue homogenate and permeabilized cells) at high oxygen concentration (tissue hyperoxia)-SUIT-018
SUIT-018 AmR mt D041D041_1GMS;2D-NS(GM)1GMS;2D;3Ama.pngAmRA: simultaneous determination of the oxygen dependence of O2 flux and H2O2 production flux in mitochondrial preparations such as isolated mitochondria, tissue homogenates and permeabilized cells (already permeabilized when they are added to the chamber) in a wide variety of species and tissues. The protocol has been designed to test the mitochondrial respiration at low oxygen levels and to modify them in the same protocol-SUIT-018
SUIT-019 O2 pfi D045D045_1PalM;2D;2c;3Oct;4P;5G;6U;7S;8Rot;9AmaFNS(PalOct,PGM)1PalM;2D;2c;3Oct;4P;5G;6U;7S;8Rot;9Ama.pngO2A SUIT-019
SUIT-020 Fluo mt D033D033_1PM;2D;3G;4S;5Rot;6Omy;7U;8AmaNS(PGM)1PM;2D;3G;4S;5Rot;6Omy;7U;8Ama.pngFluoA:short protocol for simultaneous determination of O2 flux and mitochondrial membrane potential in mitochondrial preparations (isolated mitochondria, permeabilized cells, and tissue homogenate)- SUIT-020
SUIT-020 O2 mt D032D032_1PM;2D;2c;3G;4S;5Rot;6Omy;7U;8AmaNS(PGM)1PM;2D;2c;3G;4S;5Rot;6Omy;7U;8Ama.pngO2A: short protocol for determination of O2 flux in mitochondrial preparations (isolated mitochondria, permeabilized cells, and tissue homogenate)-SUIT-020
SUIT-021 Fluo mt D036D036_1GM;2D;3S;4Rot;5Omy;6U;7AmaNS(GM)1GM;2D;3S;4Rot;5Omy;6U;7Ama.pngFluoA: short protocol for simultaneous determination of O2 flux and mitochondrial membrane potential in mitochondrial preparations (isolated mitochondria, permeabilized cells, and tissue homogenate)-SUIT-021
SUIT-021 O2 mt D035D035_1GM;2D;2c;3S;4Rot;5Omy;6U;7AmaNS(GM)1GM;2D;2c;3S;4Rot;5Omy;6U;7Ama.pngO2A:short protocol for simultaneous determination of O2 flux and mitochondrial membrane potential in mitochondrial preparations (isolated mitochondria, permabilized cells, and tissue homogenate)-SUIT-021
SUIT-022 O2 ce D051D051_ce1;ce2KCN;ce3SHAMceCe1;ce2KCN;ce3SHAM.v2.png Ce1;ce2KCN;ce3SHAM.pngO2A Determination of the respiration due to the alternative oxidase pathway -SUIT-022
SUIT-023 O2 ce D053D053_ce1;ce2SHAM;ce3KCN?Ce1;ce2SHAM;ce3KCN.pngO2A Determination of the respiration due to the cytochrome c oxidase pathway -SUIT-023
SUIT-024 O2 ce-pce D056D056_ce1;1Dig;1PM;2T;2D;3Omy;4AmaN(PM)Ce1;1Dig;1PM;2T;2D;3Omy;4Ama.pngO2Determination of the presence of ATPases in mitochondrial preparations - SUIT-024
SUIT-025 O2 mt D057D057_1D;2M;3Oct;3c;4M;5P;6G;7S;8Rot;9AmaFNS(Oct,PGM)1D;2M.1;3Oct;3c;4M2;5P;6G;7S;8Rot;9Ama.pngO2 - SUIT-025