From Bioblast
Description
Abbreviation: ?
Reference: A Determination of the respiration due to the cytochrome c pathway -SUIT-023
SUIT number: D053_ce1;ce2SHAM;ce3KCN
O2k-Application: O2
- MitoPedia: SUIT
- SUIT-category: ?
- SUIT protocol pattern: ? ce1;ce2SHAM;ce3KCN
The SUIT-023 O2 ce D053 has been designed to provide an estimation of the cyanide-and antimycin A-non-resistant mitochondrial respiration on cells on ROUTINE. To obtain a complete estimation, this protocol has to be carried out in paralel to SUIT-022 O2 ce D051
Communicated by Iglesias-Gonzalez J, Huete-Ortega M and Gnaiger E (last update 2019-04-09)
Steps and respiratory states
Step | State | Pathway | Q-junction | Comment - Events (E) and Marks (M) |
---|---|---|---|---|
ce1 | ROUTINE | ce1
| ||
ce2SHAM | ROX | ce1;ce2SHAM
| ||
ce3KCN | ROX | ce1;ce2SHAM;ce3KCN
|
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- Coupling control
- Pathway control
- Β» Electron transfer pathway
- Β» Fatty acid oxidation pathway control state, F
- Β» NADH electron transfer-pathway state, N
- Β» Succinate pathway control state, S
- Β» NS-pathway control state, NS
- Β» Glycerophosphate pathway control state, Gp
- Β» Complex IV single step, CIV
- Β» Anaplerotic pathway control state
- Pathway control
- Main fuel substrates
- Β» Glutamate, G
- Β» Glycerophosphate, Gp
- Β» Malate, M
- Β» Octanoylcarnitine, Oct
- Β» Pyruvate, P
- Β» Succinate, S
- Main fuel substrates
- Glossary
Strengths and limitations
- SUIT-023 O2 ce D053 provides an estimation of the cyanide-and antimycin A-non-resistant mitochondrial respiration on cells on ROUTINE.
- + This protocol can be used with other type of cells, although optimal concentrations of inhibitors have to be optimised.
- + Short duration of experiment
- - Estimation of the linear coupling control is not done
- - The use of cyanide requires a careful and in deep cleaning of the chambers after use and careful handling of the chemical to guarantee health safety.
Compare SUIT protocols
- SUIT-023 O2 ce D053 has to be used in parallel to obtain complete information of the alternative oxidase pathway contribution to mitochondrial respiration.
References
MitoPedia concepts: SUIT protocol, SUIT A, Find
MitoPedia methods:
Respirometry