Difference between revisions of "Template:SUIT-027 O2 ce-pce D065"

Latest revision as of 12:37, 25 November 2020

Step	State	Pathway	Q-junction	Comment - Events (E) and Marks (M)
ce1	ROUTINE			ce1 ROUTINE respiration in the physiological coupling state R. Externally added permeable substrates could contribute to this respiratory state.
1Dig	Dig			ce1;1Dig Optimum effective digitonin concentration for complete plasma membrane permeabilization.

Step	State	Pathway	Q-junction	Comment - Events (E) and Marks (M)
1M.05	(M_L)			ce1;1Dig;1M Low concentration of malate, typically low concentration, does not saturate the N-pathway, and will allow us to find the minimum concentration to stimulate the anaplerosis.
2D	( M_P)			ce1;1Dig;1M;2D OXPHOS capacity P (with saturating [ADP]), active OXPHOS state.
3M	M_P	N	CI	ce1;1Dig;1M;2D;3M NADH-linked substrates (type N-pathway to Q). OXPHOS capacity P (with saturating [ADP]), active OXPHOS state.
4P	PM_P	N	CI	ce1;1Dig;1M;2D;3M;4P NADH-linked substrates (type N-pathway to Q). OXPHOS capacity P (with saturating [ADP]), active OXPHOS state.
5G	PGM_P	N	CI	ce1;1Dig;1M;2D;3M;4P;5G NADH-linked substrates (type N-pathway to Q). OXPHOS capacity P (with saturating [ADP]), active OXPHOS state.
6Ama	ROX			ce1;1Dig;1M;2D;3M;4P;5G;6Ama Rox is the residual oxygen consumption in the ROX state, due to oxidative side reactions, estimated after addition of antimycin A (inhibitor of CIII). Rox is subtracted from oxygen flux as a baseline for all respiratory states, to obtain mitochondrial respiration (mt).

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Coupling control

» Coupling control state

Pathway control

» Electron transfer pathway

Main fuel substrates

» Glutamate, G

Glossary

» List of SUIT states

@@ Line 1: / Line 1: @@
 [[Image:SUIT-MitoFit.png|right|190px|link=http://www.bioblast.at/index.php/MitoPedia:_SUIT |MitoPedia: SUIT]]
 == Steps and respiratory states ==
-[[File:ce1;1Dig;1M;2D;3M;4P;5G.png|400px]]
+[[File:Ce1;1Dig;1M;2D;3M;4P;5G;6Ama.png|500px]]
 {{Template:ROUTINE Dig}}
@@ Line 9: / Line 9: @@
 |-
-| 1M.1
+| 1M.05
-| ([[Anaplerotic pathway control state|M]]<sub>[[LEAK|''L'']]</sub>)
+| ([[Malate-anaplerotic pathway control state|M]]<sub>[[LEAK respiration|''L'']]</sub>)
-| ([[N]])
+|
-| CI
+|
 | ce1;1Dig;1M
-*{{Template:SUIT M_low}}
+*Low concentration of [[malate]], typically low concentration, does not saturate the [[N-pathway]], and will allow us to find the minimum concentration to stimulate the anaplerosis.
 |-
 | 2D
-| ([[Anaplerotic pathway control state| M]]<sub>[[OXPHOS|''P'']]</sub>)
+| ([[Malate-anaplerotic pathway control state| M]]<sub>[[OXPHOS|''P'']]</sub>)
-| ([[N]])
+|
-| CI
+|
 | ce1;1Dig;1M;2D
 *{{Template:SUIT OXPHOS}}
@@ Line 26: / Line 26: @@
 |-
 | 3M
-| [[Anaplerotic pathway control state| M]]<sub>[[OXPHOS|''P'']]</sub>
+| [[Malate-anaplerotic pathway control state| M]]<sub>[[OXPHOS|''P'']]</sub>
 | [[N]]
 | CI
@@ Line 44: / Line 44: @@
 |-
 | 5G
-| [[PM]]<sub>[[OXPHOS|''P'']]</sub>
+| [[PGM]]<sub>[[OXPHOS|''P'']]</sub>
 | [[N]]
 | CI
@@ Line 59: / Line 59: @@
 *{{Template:SUIT Ama}}
 |}
+{{Keywords: SUIT protocols}}
-{{Template:Keywords in SUIT protocols}}