SUIT-023 O2 ce D053

high-resolution terminology - matching measurements at high-resolution

SUIT-023 O2 ce D053

Description

Abbreviation: ?

Reference: A Determination of the respiration due to the cytochrome c oxidase pathway -SUIT-023

SUIT number: D053_ce1;ce2SHAM;ce3KCN

O2k-Application: O2

MitoPedia: SUIT

SUIT-category: ce

SUIT protocol pattern: linear ce1;ce2SHAM;ce3KCN

The SUIT-023 O2 ce D053 has been designed to provide an estimation of the mitochondrial respiration in microalgal intact cells due to the cytochrome c oxidase pathway, which is non-resistant to cyanide-and antimycin A inhibition. This protocol must be complemented with the study in parallel of the mitochondrial respiration due to the alternative oxidase pathway (see SUIT-022).

Communicated by Huete-Ortega M and Gnaiger E (last update 2019-04-09)

Strengths and limitations

• SUIT-023 O2 ce D053 provides an estimation of the cyanide-and antimycin A-non-resistant mitochondrial respiration in microalgal intact cells.
• The concentrations of inhibitors used in this protocol have been optimised for the specific case of the microalgal model species Chlamydomonas reinhardtii

+ Short duration of experiment

- Estimation of the linear coupling control is not done

- The use of cyanide requires a careful and in deep cleaning of the chambers after use and careful handling of the chemical to guarantee health safety.

Compare SUIT protocols

• SUIT-022 O2 ce D051 has to be used in parallel to obtain complete information of the contribution of both alternative oxidase and cytochrome c oxidase pathways to mitochondrial respiration.

References

MitoPedia concepts: SUIT protocol, SUIT A, Find 

MitoPedia methods: Respirometry