Difference between revisions of "SUIT-013"
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::: '''[[Categories of SUIT protocols |SUIT-category]]:''' | ::: '''[[Categories of SUIT protocols |SUIT-category]]:''' ce | ||
::: '''[[SUIT protocol pattern]]:''' | ::: '''[[SUIT protocol pattern]]:''' linear | ||
__TOC__ | __TOC__ | ||
Communicated by [[Komlodi T]] (last update 2019-01-22) | Communicated by [[Komlodi T]] (last update 2019-01-22) |
Revision as of 17:49, 22 January 2019
Description
Abbreviation: ce
Reference: A »Versions
- SUIT-category: ce
- SUIT protocol pattern: linear
Communicated by Komlodi T (last update 2019-01-22)
DLP applications
References
Steps and respiratory states
Step | State | Pathway | Q-junction | Comment - Events (E) and Marks (M) |
---|---|---|---|---|
0DTPA |
| |||
0SOD |
| |||
0HRP |
| |||
0AmR |
|
Step | State | Pathway | Q-junction | Comment - Events (E) and Marks (M) |
---|---|---|---|---|
ce1 | ROUTINE | ce1
|
- Bioblast links: SUIT protocols - >>>>>>> - Click on [Expand] or [Collapse] - >>>>>>>
- Coupling control
- Pathway control
- Main fuel substrates
- » Glutamate, G
- » Glycerophosphate, Gp
- » Malate, M
- » Octanoylcarnitine, Oct
- » Pyruvate, P
- » Succinate, S
- Main fuel substrates
- Glossary
Strengths and limitations
- A succinate concentration > 10 mM might be required to reach saturating S-OXPHOS capacity.
- In some cell lines e.g. HEK293T, P does not have any effect because of the downregulation of the N-linked pathway.
- + Contribution of N pathway to the NS can be checked after the addition of Rot.
- + Reasonable duration of the experiment.
- + This protocol can be extended with the Complex IV module.
- - Rox may be lower in substrate states earlier in the SUIT protocol. Therefore, this Rox measurement is frequently taken as a methodological control rather than as the final basis of Rox correction of mitochondrial respiration (mt).
- - Careful washing is required after the experiment to avoid carry-over of inhibitors.
Compare SUIT protocols
MitoPedia concepts: MiP concept, SUIT protocol, Recommended
MitoPedia methods:
Respirometry,
Fluorometry