Difference between revisions of "SUIT-003 O2 ce D061"
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== Strengths and limitations == | == Strengths and limitations == | ||
:::* The [[SUIT-003 O2 ce D061]] is a cell [[coupling control protocol]]. The total cell count (''N''<sub>ce</sub>) is the sum of viable cells (''N''<sub>vce</sub>) and dead cells (''N''<sub>dce</sub>). | :::* The [[SUIT-003 O2 ce D061]] is a cell [[coupling control protocol]]. The total cell count (''N''<sub>ce</sub>) is the sum of viable cells (''N''<sub>vce</sub>) and dead cells (''N''<sub>dce</sub>). | ||
:::* [[CIV]] activity can also be measured after the cell viability test module. | :::* [[CIV]] activity can also be measured after the cell viability test module. |
Revision as of 10:08, 26 June 2019
Description
Abbreviation: ce
Reference: B - complex cell coupling control protocol (CCP) with the Prodrug of malonate NV161-SUIT-003
- SUIT protocol pattern: ce1;ce2Omy;ce3U;ce4Rot;ce5S;ce6Mnanv;ce7Ama
SUIT-003 O2 ce-pce D061 is designed to study coupling control and the presence of succinate transporters in the plasma membrane of living cells. Respiratory capacities are tested in a sequence of coupling states: ROUTINE, LEAK and ET. To study LEAK-respiration, the phosphorylation system is inhibited by oligomycin. The final concentration of oligomycin has to be carefully optimized for various cell types, to minimize the inhibitory effect on the electron transfer system which would lead to an underestimation of ET-capacity. The addition of succinate after rotenone will allow us to stimulate the respiration in living cells expressing a succinate transporter in the plasma membrane and those cells that present a damage plasma membrane and are permeable to the substrate. However, the use of the Prodrug of malonate (Mnanv) allows us to differentiate between these two possible sources of stimulation in the respiration. The permeable compounds only work in living cells which a non-altered plasma membrane due that to be active the cytosolic esterases must cleavage the compound. The living cells with an altered permeability of the plasma membrane will not be affected by the used of the Prodrug of malonate (Mnanv).
Communicated by Garcia-Souza LF and Gnaiger E(last update 2019-06-24)
Steps and respiratory states
Step | State | Pathway | Q-junction | Comment - Events (E) and Marks (M) |
---|---|---|---|---|
ce1 | ROUTINE | ce1
| ||
(ce2Omy) | L(Omy) | ce1;(ce2Omy)
| ||
ce3U | E | cce1;(ce2Omy);ce3U
| ||
ce4Rot | ROX | ce1;(ce2Omy);ce3U;ce4Rot
| ||
ce5DMSO | ROX | ce1;(ce2Omy);ce3U;ce4Rot;ce5DMSO
| ||
ce6DMSO | ROX | ce1;(ce2Omy);ce3U;ce4Rot;ce5DMSO;ce6DMSO
| ||
ce7Ama | ROX | ce1;(ce2Omy);ce3U;ce4Rot;ce5DMSO;ce6DMSO;ce7Ama
|
Strengths and limitations
- The SUIT-003 O2 ce D061 is a cell coupling control protocol. The total cell count (Nce) is the sum of viable cells (Nvce) and dead cells (Ndce).
- CIV activity can also be measured after the cell viability test module.
- - The addition of oligomycin has to be carefully tested to avoid inhibitory effects in the evaluation of the ET-capacity.
- - Careful washing is required after the experiment to avoid carry-over of inhibitors and uncoupler.
Compare SUIT protocols
- SUIT-003 O2 ce D060 CCP with cell permeable compounds NV118 and NV161
- SUIT-003 O2 ce D028 for cell membrane permeability test CCP(S).
- SUIT-003 O2 ce-pce D013 first version of the Coupling control protocol and respirometric cell viability test (CCV protocol).
- SUIT-003 O2 ce-pce D018 second version of the Coupling control protocol and respirometric cell viability test (CCV protocol).
- SUIT-003 Ce1;ce1P;ce3U;ce4Glc;ce5M;ce6Rot;ce7S;1Dig;1c;2Ama;3AsTm;4Azd coupling control protocol and respirometric cell viability test (CCV protocol) without oligomycin.
- SUIT-003 Ce1;ce2U-
- SUIT-003 Ce1;ce2SD;ce3Omy;ce4U-
- SUIT-003 Ce1;ce2SD;ce3U;ce4Rot;ce5Ama
- SUIT-003 Ce1;ce2U;ce3Rot;ce4S;ce5Ama
- SUIT-006: Coupling control protocol for mt-preparations (mtprep), counterpart to SUIT-003 for non-permeabilized cells (ce).
References
MitoPedia concepts: SUIT protocol, SUIT B, Find
MitoPedia methods:
Respirometry