From Bioblast
- high-resolution terminology - matching measurements at high-resolution
SUIT-003 Ce1;ce2SD;ce3Omy;ce4U-
Description
Abbreviation: FNS(Oct,PGM)
Reference: B SUIT-003 Stadlmann 2006 Cell Biochem Biophys
O2k-Application: O2
- SUIT protocol pattern: complex cell Coupling-control protocol ce1;ce2SD;ce3Omy;ce4U;ce5Rot;ce6Ama
Coupling-control protocol
- 1ce;2ceSD;3ceOmy;4ceU;5ceRot;6ceAma
Step | Respiratory state | Pathway control | Comment |
---|---|---|---|
ce1 | ROUTINE | Iiving cells (ce) | Dead cells (dce) without substrate are in a state of ROX. |
ce2SD | R+SP | ROUTINE (R) + S (SP) | dce with functional mitochondria are in state SP. |
ce3Omy | L+SL | LEAK respiration (L) + S (SL) | ce and dce are in the LEAK state. |
ce4U | E+SE | Electron transfer pathway (E) + S (SE) | ce and dce are in the ET state. |
ce5Rot | ROX+SE | ROX+S (SE) | ce are in the ROX state, dce are in the ET state. |
ce6Ama | ROXAma | ROX |
- ce1;ce1S .. versus ce1;ce2Omy;ce3U;ce4S .. and respiration medium
- Living cells & succinate: ADP is not permeable through the plasma membrane of living cells, and this is the case for succinate in many cell types (Steinlechner-Maran 1997 Transplantation). If succinate and ADP are accessible to mitochondria in a fraction of permeabilized (non-viable) cells, respiration will be stimulated, if these mitochondria retain a functional S-pathway (CII-linked respiration). Preservation of mitochondrial respiratory function provides the argument of using mitochondrial respiration medium (MiR05) in this CCP with cells of limited viability (Stadlmann_2006_Cell Biochem Biophys).
MitoPedia concepts:
SUIT protocol,
SUIT B