MiPNet14.06 Instrumental O2 background

O2k Quality Control 2: Instrumental oxygen background correction and accuracy of oxygen flux.

»  » Versions

Oroboros (2020-04-16) Mitochondr Physiol Network

Abstract: Fasching M, Gnaiger E (2020) O2k Quality Control 2: Instrumental oxygen background correction and accuracy of oxygen flux. Mitochondr Physiol Network 14.6(08):1-16.

Open the pdf document above.

» Current O2k-series: NextGen-O2k Series XB and O2k Series J

» Current software versions DatLab 8.0: MitoPedia: DatLab

• Further details: » MitoPedia: O2k-Open Support

» Product: O2k-FluoRespirometer, O2k-Catalogue

• O2k-Network Lab: AT_Innsbruck_Oroboros

Labels: MiParea: Respiration, Instruments;methods 

HRR: Oxygraph-2k, O2k-Manual 

DatLab, O2k-SOP, DL7, System, MitoFit 2021 PT 

                  

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MiPNet14.06 Instrumental O2 background

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Keywords

»Bioblast links: Chamber volume - >>>>>>> - Click on [Expand] or [Collapse] - >>>>>>>

» Volume

• Units: Liter [L]; 1 L = 1 dm³ = 10^-3 m³; 1 mL = 1 cm³; 1 µL = 1 mm³

» O2k-chamber

» The experimental chamber in the instrumental O2k-Chamber

» O2k-Chamber sV

» Calibration of O2k-chamber volume

• On chamber volume

» O2k-chamber

» Chamber volume

» MiPNet09.01 O2k-ParadigmShift

» Cell count and normalization in HRR

» Open chamber

» Closed chamber

• Components of the experimental O2k-chambers

» Stirrer-Bar\white PVDF\15x6 mm

» Stirrer-Bar sV\white PVDF\11.5x6.2 mm

» Stopper\black PEEK\conical Shaft\central Port

» Stopper sV\black PEEK\conical Shaft\central Port

» O-ring\Viton\12.5x1 mm

» O-ring sV\Viton\9.5x1 mm

» Volume-Calibration Ring

» Volume-Calibration Ring sV

» Stopper-Spacer

» Cover-Slip\black

» O2k-Chamber Holder

» O2k-Chamber Holder sV

» OroboPOS-Holder

» OroboPOS-Holder sV

• Maintencance

» O2k-cleaning and ISS

» Quality control: O2k-chamber test

Acknowledgements

Cited by

• Komlódi T, Cardoso LHD, Doerrier C, Moore AL, Rich PR, Gnaiger E (2021) Coupling and pathway control of coenzyme Q redox state and respiration in isolated mitochondria. Bioenerg Commun 2021.3. https://doi.org/10.26124/bec:2021-0003