Difference between revisions of "User:Spitzer Gudrun/SV-Chamber Instructions"
From Bioblast
| (5 intermediate revisions by the same user not shown) | |||
| Line 10: | Line 10: | ||
* [[Glucose]]: second paragraph is not in titrations list, leave it? | * [[Glucose]]: second paragraph is not in titrations list, leave it? | ||
* [[Glycerophosphate]] | * [[Glycerophosphate]] | ||
* [[Carnitine]] | |||
=== Different concentration for different chambers === | === Different concentration for different chambers === | ||
| Line 31: | Line 32: | ||
* [[Carboxyatractyloside]] deleted higher concentration because not recommended any more | * [[Carboxyatractyloside]] deleted higher concentration because not recommended any more | ||
* [[Oligomycin]] | * [[Oligomycin]] | ||
* [[FCCP]] | |||
* [[Ruthenium_red]] | |||
* [[SF6847]] | |||
== | == no sV-instructions == | ||
* [[Salicylhydroxamic acid]] | * [[Salicylhydroxamic acid]] because only used for algae. | ||
* [[Ap5A]] because not given by Luiza | |||
Latest revision as of 09:17, 17 May 2021
I Added sV Instructions to following pages
Same concentration for standard and small volume chamber
- Malate
- Glutamate
- Succinate
- Octanoylcarnitine
- ADP
- ATP
- Glucose: second paragraph is not in titrations list, leave it?
- Glycerophosphate
- Carnitine
Different concentration for different chambers
Concentrations prepared separately
- Pyruvate
- Ascorbate
- TMPD
- Cytochrome_c
- KCN is the amount of stock solution and volume of aliquote ok?
- Digitonin
- Catalase why are different hamilton syringe sizes indicated on the table although the titration volume is the same for standard- and sV-chamber?
- Hydrogen peroxide: preparation and titration are described on MiPNet14.13_Medium-MiR06#Re-oxygenation_with_H2O2_titrations
Conc for sV-chamber by dilution
- CCCP
- Rotenone
- Malonate
- Antimycin A
- Myxothiazol
- Sodium azide
- Carboxyatractyloside deleted higher concentration because not recommended any more
- Oligomycin
- FCCP
- Ruthenium_red
- SF6847
no sV-instructions
- Salicylhydroxamic acid because only used for algae.
- Ap5A because not given by Luiza
