Difference between revisions of "Template:SUIT-019"
Line 9: | Line 9: | ||
| 1PalM | | 1PalM | ||
| [[PalM]]<sub>''L''</sub> | | [[PalM]]<sub>''L''</sub> | ||
| [[F(N)]] | | [[F(N)|Fatty acid oxidation pathway control state]] | ||
| CETF | | CETF | ||
| PalM<sub>''L''</sub>: Palmitoylcarnitine & malate, F-LEAK respiration, F<sub>''L''</sub> | | PalM<sub>''L''</sub>: Palmitoylcarnitine & malate, F-LEAK respiration, F<sub>''L''</sub> | ||
Line 18: | Line 18: | ||
| 2D | | 2D | ||
| [[PalM]]<sub>''P''</sub> | | [[PalM]]<sub>''P''</sub> | ||
| [[F(N)]] | | [[F(N)|Fatty acid oxidation pathway control state]] | ||
| CETF | | CETF | ||
| 1PalM;2D | | 1PalM;2D | ||
Line 27: | Line 27: | ||
| 3Oct | | 3Oct | ||
| [[PalOctM]]<sub>''P''</sub> | | [[PalOctM]]<sub>''P''</sub> | ||
| [[F(N)]] | | [[F(N)|Fatty acid oxidation pathway control state]] | ||
| CETF | | CETF | ||
| 1PalM;2D;3Oct | | 1PalM;2D;3Oct |
Revision as of 17:08, 22 January 2019
Steps and respiratory states
Step | State | Pathway | Q-junction | Comment - Events (E) and Marks (M) |
---|---|---|---|---|
1PalM | PalML | Fatty acid oxidation pathway control state | CETF | PalML: Palmitoylcarnitine & malate, F-LEAK respiration, FL
Respiratory stimulation of the FAO-pathway, F, by fatty acid, FA, in the presence of malate, M. Malate is a type N substrate (N), required for the F-pathway. The FA concentration has to be optimized to saturate the F-pathway, without inhibiting or uncoupling respiration. Template:SUIT L n |
2D | PalMP | Fatty acid oxidation pathway control state | CETF | 1PalM;2D
Respiratory stimulation of the FAO-pathway, F, by fatty acid, FA, in the presence of malate, M. Malate is a type N substrate (N), required for the F-pathway. The FA concentration has to be optimized to saturate the F-pathway, without inhibiting or uncoupling respiration. OXPHOS capacity P (with saturating [ADP]), active OXPHOS state. |
3Oct | PalOctMP | Fatty acid oxidation pathway control state | CETF | 1PalM;2D;3Oct
Respiratory stimulation of the FAO-pathway, F, by fatty acid, FA, in the presence of malate, M. Malate is a type N substrate (N), required for the F-pathway. The FA concentration has to be optimized to saturate the F-pathway, without inhibiting or uncoupling respiration. OXPHOS capacity P (with saturating [ADP]), active OXPHOS state. |
4P | PalOctPMP | FN | CETF&I | 1PalM;2D;3Oct;4P
Respiratory stimulation of the FAO-pathway, F, by fatty acid, FA, in the presence of malate, M. Malate is a type N substrate (N), required for the F-pathway. The FA concentration has to be optimized to saturate the F-pathway, without inhibiting or uncoupling respiration. & NADH-linked substrates (type N-pathway to Q). Respiratory stimulation by simultaneous action of the F-pathway and N-pathway with convergent electron flow in the FN-pathway for evaluation of an additive or inhibitory effect of F. OXPHOS capacity P (with saturating [ADP]), active OXPHOS state. |
5G | PalOctPGMP | FN | CETF&I | 1PalM;2D;3Oct;4P;5G
Respiratory stimulation of the FAO-pathway, F, by fatty acid, FA, in the presence of malate, M. Malate is a type N substrate (N), required for the F-pathway. The FA concentration has to be optimized to saturate the F-pathway, without inhibiting or uncoupling respiration. & NADH-linked substrates (type N-pathway to Q). Respiratory stimulation by simultaneous action of the F-pathway and N-pathway with convergent electron flow in the FN-pathway for evaluation of an additive or inhibitory effect of F. OXPHOS capacity P (with saturating [ADP]), active OXPHOS state. |
6U | PalOctPGME | FN | CETF&CI&II | 1PalM;2D;3Oct;4P;5G;6U
Respiratory stimulation of the FAO-pathway, F, by fatty acid, FA, in the presence of malate, M. Malate is a type N substrate (N), required for the F-pathway. The FA concentration has to be optimized to saturate the F-pathway, without inhibiting or uncoupling respiration. & NADH-linked substrates (type N-pathway to Q). Respiratory stimulation by simultaneous action of the F-pathway and N-pathway with convergent electron flow in the FN-pathway for evaluation of an additive or inhibitory effect of F. Uncoupler titration (avoiding inhibition by high uncoupler concentrations) to obtain electron transfer (ET) capacity E (noncoupled ET-state). Test for limitation of OXPHOS capacity P by the phosphorylation system (ANT, ATP synthase, phosphate transporter) relative to ET capacity E in mt-preparations: E-P control efficiency and E-L coupling efficiency. In living cells: E-R control efficiency and E-L coupling efficiency. Noncoupled electron transfer state, ET state, with ET capacity E. |
7S | PalOctPGMSE | FNS | CI&CII | 1PalM;2D;3Oct;4P;5G;6U;7S
Respiratory stimulation of the FAO-pathway, F, by fatty acid, FA, in the presence of malate, M. Malate is a type N substrate (N), required for the F-pathway. The FA concentration has to be optimized to saturate the F-pathway, without inhibiting or uncoupling respiration. & NADH-linked substrates (type N-pathway to Q). Respiratory stimulation by simultaneous action of the F-pathway and N-pathway with convergent electron flow in the FN-pathway for evaluation of an additive or inhibitory effect of F. Succinate, S ( type S-pathway to Q). Noncoupled electron transfer state, ET state, with ET capacity E. |
8Rot | SE | S | CII | 1PalM;2D;3Oct;4P;5G;6U;7S
Succinate pathway control state (S-pathway) after inhibiting CI with rotenone, which also inhibits the F-pathway. Succinate, S ( type S-pathway to Q). Noncoupled electron transfer state, ET state, with ET capacity E. |
Step | Respiratory state | Pathway control | ET-Complex | Comment |
---|---|---|---|---|
## AsTm | AsTmE | CIV | CIV | |
## Azd | CHB |
- Bioblast links: SUIT protocols - >>>>>>> - Click on [Expand] or [Collapse] - >>>>>>>
- Coupling control
- Pathway control
- Main fuel substrates
- » Glutamate, G
- » Glycerophosphate, Gp
- » Malate, M
- » Octanoylcarnitine, Oct
- » Pyruvate, P
- » Succinate, S
- Main fuel substrates
- Glossary