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...are designed to measure simultaneously O₂ flux and the Q-redox state in isolated mitochondria and permeabilized cells. ...t D072]] simultaneous determination of O₂ flux and the Q-redox state in isolated mitochondria and cells permeabilized previously to addition to

...er electron transfer pathways are also analyzed in OXPHOS (FN, FNS) and ET-state (FNS, S). :::+ The protocol provides information on F-pathway in OXPHOS state with palmitoylcarnitine, a long-chain acylcarnitine commonly found ''in viv

...er electron transfer pathways are also analyzed in OXPHOS (FN, FNS) and ET-state (FNS, S). :::+ The protocol provides information on F-pathway in OXPHOS state with palmitoylcarnitine, a long-chain acylcarnitine commonly found ''in viv

...r W (2016) Adapting high-resolution respirometry to glucose-limited steady state mycelium of the filamentous fungus ''Penicillium ochrochloron'': method dev ...lium and a direct transfer of a broth sample into the respirometer. Steady state respiration was 29% below its maximum respiratory capacity. Additionally to

...of the Q-junction, respectively, impacts the relation between the Q redox state and O₂ flux in opposite directions. Therefore, our aim was to ch ...he NextGen-O2k with Q-Module to measure O₂ flux and the Q redox state simultaneously, using CoQ₂ as a probe [3]. Cryopreserved HEK 293

...tate| S]] and [[NADH Electron transfer-pathway state| N-]] pathway control state. ...s to be determined. Higher concentrations of oligomycin may inhibit the ET state.

...O₂ flux, H₂O₂ flux, and coenzyme Q redox state. AussieMit 2018 Melbourne AU. ...an altered Q redox state. We analyzed the relationship between the Q redox state, H₂O₂ and O₂ fluxes with fuel substrates p

...coli leads to a corresponding decrease in the enzyme's operational steady-state affinity for O2. This demonstrates directly that fast electron transfer bet

...Rich PR, Gnaiger E (2021) Coupling and pathway control of coenzyme Q redox state and respiration in isolated mitochondria. https://doi.org/10.26124/mitofit: ...omlodi_2021_MitoFit_Q.pdf Coupling and pathway control of coenzyme Q redox state and respiration in isolated mitochondria]

...getic fingerprinting with coupling and pathway control of coenzyme Q redox state and respiration in permeabilized HEK 293T cells. 56^th Annual Sci We characterised the Q redox state in pathway and coupling control using the Oroboros NextGen-O2k with the ele

! Respiratory state ...ate with type N substrates, N_''L'': Non-phosphorylating resting state with NADH-linked (type N) substrates pyruvate&glutamate&malate (PGM; withou

...support NADH-linked respiration. Oligomycin (Omy) is used to induce a LEAK state of respiration via the inhibition of the ATP synthase. Since higher concent ...sed [[Mitochondrial membrane potential|mt-membrane potential]] in the LEAK state.

...that supports succinate-linked respiration (succinate-induced respiratory state; previously used nomenclature: CII-linked respiration; SRot; see [[Gnaiger The S-pathway control state is usually induced in mt-preparations by addition of succinate&rotenone. In

|abbr=''ET-pathway state'' ...state]]s. [[Coupling-control state]]s require [[Electron-transfer-pathway state|ET-pathway competent states]], including oxygen supply. [[Categories of SUI

...f the ATP synthase. Since higher concentrations of Omy can decrease the ET state induced upon addition of uncoupler, the required concentration of Omy has t ...sed [[Mitochondrial membrane potential|mt-membrane potential]] in the LEAK state.

...f the ATP synthase. Since higher concentrations of Omy can decrease the ET state induced upon addition of uncoupler, the required concentration of Omy has t ...sed [[Mitochondrial membrane potential|mt-membrane potential]] in the LEAK state.

...ate]] of respiration, whereas the cytochromes are in a more oxidized redox state. |mitopedia concept=Respiratory state, Find

...(NS > S). However, in some types of mitochondria, rotenone added in the NS-state elicits a paradoxical increase in ''J''_O₂, revealing ::::# Department of Biomedical Sciences, Colorado State University, Fort Collins, Colorado, USA - [email protected]

...in the LEAK state. Pyruvate (P) supports [[NADH Electron transfer-pathway state|NADH-pathway (N)]], and usually in the presence of ADP it does not increase ...s protocol is designed to evaluate RET in the S-linked pathway in the LEAK state, which is decreased by ADP due to reduction of the mt-membrane potential.

...s: Continuous monitoring of pull and push control of respiration and redox state of the Q-mimetic CoQ₂. Q10 Hamburg. ...mplemented into the Oroboros NextGen-O2k to monitor continuously the redox state of CoQ2 added as a Q-mimetic simultaneously with O2 consumption (Komlódi e

...without the involvement of the [[complex II]] ([[Succinate pathway control state| S-pathway]]). It can be useful to understand the contribution and the acti ...pathway state| N-pathway]] is lower and of the [[Succinate pathway control state| S-pathway]] is higher with GM compared to PM (GMP is inhibited by the CII

Let's face it: even the state-of-the-art terminology and the many inconsistent variations are not commonl An even worse mistake: we say ‘[[Complex I&II-linked substrate state |CI<small>&</small>II]]’ (or a previous mistake: ‘CI+II’: ''are the t

...nterpart of the soluble mitochondrial cytochrome c, in a so-called "closed state," in which electrons are translocated from the bcc to the aa3 domain. The s

...-[[ET capacity| ''E'']]) with NADH-linked substrates ([[PM-pathway control state|PM]] and PGM)''' ...may be relevant in the presence of [[glutamate-anaplerotic pathway control state]]. SUIT-012 can be extended with the CIV assay module.

...n level of pyridine nucleotide than was observed aerobically in state 4. These observations suggest that the presence of adenosine 5’

...Rich PR, Gnaiger E (2021) Coupling and pathway control of coenzyme Q redox state and respiration in isolated mitochondria. Bioenerg Commun 2021.3. https://d ...same manner as Q at Complexes CI, CII and CIII. The CoQ₂ redox state is monitored amperometrically with the working electrode, which is poised a

...f mitochondrial respiration, H₂O₂ flux and the redox state of the Q-pool. ...Q-pool [1]. We aimed at investigating the relationship between the Q redox state, H₂O₂ and O₂ fluxes with various fuel subs

...combinations for N-pathway control state; S or RotS for S-pathway control state.

...ty of the cyanide-resistant pathway. The relation between rate and Q redox state for the external NADH dehydrogenase in potato callus mitochondria was found ...spiration rate is proportional to the redox state of the Q-pool. - ''redox state expressed as QH₂ fraction''

...nitoring of respiration together with NAD(P)H autofluorescence and Q-redox state [1] provides unique analytical and diagnostic power in the study of mitocho ...Rich PR, Gnaiger E (2021) Coupling and pathway control of coenzyme Q redox state and respiration in isolated mitochondria. https://doi.org/10.26124/bec:2021

...ectron transfer. Further, the short-lived anionic flavin semiquinone (ASQ) state of b-FAD is essential for electron bifurcation. Nfn homologs are widely dis

...is necessary to prevent a large decrease in oxygen affinity in the active state. Upregulation of oxygen affinity provides a functional explanation of the e ...t from definitions by Chance, Williams 1955) has the meaning of a [[LEAK]] state of respiration, without added adenylates (no ADP and no ATP) in the presenc

...herapies. Here, we have investigated the impact of mitochondrial metabolic state on the probability and consequences of mPTP opening. Isolated mitochondria

...reduces the Q pool almost entirely (>90 %) irrespective of the respiratory state. AOX enhances the forward electron transfer (FET) from CII thereby decreasi |topics=Redox state, Q-junction effect

...s dramatically. Pyruvate (P) supports the [[NADH Electron transfer-pathway state|NADH-pathway]] and thus the convergent NS-pathway with little further effec ...s protocol is designed to evaluate RET in the S-linked pathway in the LEAK state, which is decreased by ADP due to reduction of the protonmotive force, ''pm

...tate appropriately. Since higher concentrations of Omy can decrease the ET state induced upon addition of uncoupler, the required concentration of Omy has t ...tential measurements, choose [[SUIT-006 Fluo mt D034]]. For O2 and Q redox state measurements, choose [[SUIT-006 Q mt D071]] or [[SUIT-006 Q ce-pce D073]].

...te in the matrix as substrates for NS-pathway control. This physiological state is reconsituted in mitochondrial preparations by external NS-substrate supp :::::* MitoPedia:» [[Electron-transfer-pathway state#Terminology: from CI- and CII-linked to N and S]]

...lision model) and Q tightly bound to supercomplexes according to the solid-state model. The two models describe the extremes in a continuum of homogenous or ..._hom || homogenous pool of ETS-reactive Q according to the fluid-state model (random-collision model) || || 1, 4, 5

...hich leads to an overestimation of LEAK respiration, when comparing [[LEAK state without adenylates|''L''(n)]] and [[LEAK respiration|''L''(Omy)]]. Tissue h ...ther N-pathway substrate combinations; S or RotS for the S-pathway control state.

...iacetate. ROS production by Complex I is strictly related to its inhibited state. Our results indicate that different Complex I inhibitors can be grouped in

...n isolated mitochondria, myotubes and exercising humans. Performing steady-state respirometry with isolated mitochondria we found that oxygen limitation of |topics=Calcium, mt-Membrane potential, Oxygen kinetics, Redox state

...ations of succinate and is thought not to occur when NAD is in the reduced state. However, very few studies have examined the rates of superoxide production

...the SUIT-003 O2 ce D061 protocol allows evaluating the [[coupling control state |coupling control]] of living cells. Respiratory capacities are tested in a

...n in the LEAK state. Pyruvate (P) promotes[[NADH Electron transfer-pathway state|NADH-pathway (N)]], and usually in the presence of ADP, H₂O<sub ...ub>2</sub>O₂ flux on the mt-membrane potantial on the N-control state in isolated mitochondria, tissue homogenate and permeabilized cells (alread

...ects on oxidative phosphorylation, mitochondrial membrane potential, redox state of coenzyme Q, and calcium uptake. EU-METAHEART MC2 Meeting 2024 Antalya TR ...₂ levels in studies of isolated mitochondria. Performing steady-state respirometry with isolated mouse cardiac mitochondria [2] we found that oxy

...sed [[Mitochondrial membrane potential|mt-membrane potential]] in the LEAK state. ...tate| S]] and [[NADH Electron transfer-pathway state| N-]] pathway control state.

...ni''. We observed that the oxygen consumption associated with the electron transfer-pathway (ET-pathway) is significantly lower in females than in males. This phenomen |topics=Aerobic glycolysis, Redox state

...n2+ inhibition of complex I is high (10-50 microm, depending on the enzyme state); therefore, complex I is unlikely to be a major site for zinc inhibition o

...e ET pathways with FNSGpCIV. Schematic representation of multiple electron transfer-pathway (ET-pathway) pathways. Electrons converge at the Q-junction from Complex I ...ol and the subsequent linear sequence of pathway control in the ET-pathway state. RP2) RP2 has a focus on OXPHOS capacity of fatty acid oxidation (F-pathway

...b>2</sub></sub> || 1 || P»/O₂ ratio measured in any respiratory state; Section 1.6; Eq. 3.4 | coupling control state || [[Coupling control state]] || CCS || - || Section 2.4.1