Difference between revisions of "SUIT-022"

Revision as of 11:09, 9 April 2019

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SUIT-022

Description

Reference: A: Determination of the respiration due to the alternative oxidase pathway »Versions

O2k-Application: O2

SUIT-category: ce

SUIT protocol pattern: linear ce1;ce2KCN;ce3SHAM

The SUIT-022 is designed to provide an estimation of the mitochondrial respiration in cells due to the alternative oxidase pathway, which is cyanide-and antimycin A-resistant. This protocol must be complemented with the study in parallel of the mitochondrial respiration due to the cytochrome c pathway (see SUIT-023.

Communicated by Huete-Ortega M and Gnaiger E (last update 2019-04-09)

Specific SUIT protocols

SUIT-022 O2 ce D051

SUIT-022 O2 ce D051 for intact microalgal cells (ce)

Strengths and limitations

SUIT-022 provides an estimation of the cyanide-and antimycin A-resistant mitochondrial respiration due to the activity of the alternative oxidase on cells on ROUTINE. To complement the study with the estimation of the contribution of the cytochrome c pathway to respiration, it must be carried out in parallel to SUIT-023.

+ This protocol can be used with other type of cells, although optimal concentrations of inhibitors have to be optimised.

+ Short duration of experiment

- Estimation of the linear coupling control is not done

- The use of cyanide requires a careful and in deep cleaning of the chambers after use and careful handling of the chemical to guarantee health safety.

Compare SUIT protocols

References

MitoPedia concepts: MiP concept, SUIT protocol, Recommended

MitoPedia methods: Respirometry

@@ Line 3: / Line 3: @@
 |application=O2
 }}
-::: '''[[Categories of SUIT protocols |SUIT-category]]:'''
+::: '''[[Categories of SUIT protocols |SUIT-category]]:''' ce
 ::: '''[[SUIT protocol pattern]]:''' linear ce1;ce2KCN;ce3SHAM
-The SUIT-022 is designed to provide an estimation of the [[cyanide| cyanide]]-and [[Antimycin_A| antimycin A]]-resistant mitochondrial respiration due to the activity of the [[alternative oxidase]] on cells on [[ROUTINE]]. To obtain a complete estimation of the contribution of both the alternative oxidase and the cytochrome c pathways, both [[SUIT-022 O2 ce D051]] and [[SUIT-023 O2 ce D053]] must be carried out in parallel.
+The SUIT-022 is designed to provide an estimation of the mitochondrial respiration in cells due to the [[alternative oxidase]] pathway, which is [[cyanide| cyanide]]-and [[Antimycin_A| antimycin A]]-resistant. This protocol must be complemented with the study in parallel of the mitochondrial respiration due to the cytochrome c pathway (see [[SUIT-023]].
 __TOC__
-  Communicated by [[Iglesias-Gonzalez J]], [[Huete-Ortega M]] and [[Gnaiger E]] (last update 2019-04-04)
+  Communicated by [[Huete-Ortega M]] and [[Gnaiger E]] (last update 2019-04-09)
 == Specific SUIT protocols ==
@@ Line 17: / Line 18: @@
 [[File:D051_O2_traces.png|400px]]
 *  [[SUIT-022 O2 ce D051]] for intact microalgal cells (ce)
-=== SUIT-022 O2 ce D00SHAM ===
-[[File:ce1;ce2SHAM;ce3KCN.png|400px]]
-[[File:D053_O2_traces.png|400px]]
-*  [[SUIT-023 O2 ce D053]] for for intact microalgal cells (ce)
 {{Template:SUIT-022}}