O2k-Virtual Support self-study material: Difference between revisions

Latest revision as of 12:50, 7 November 2023

O2k-Virtual Support self-study material

MitoPedia O2k and high-resolution respirometry: O2k-Open Support

General information

To benefit optimally from the tutored training sessions of the O2k-Virtual Support, please make yourself familiar with Materials for self-study in advance.

O2k-Basic

Part 1.1: OroboPOS service and O2k instrumental

O2k-Manual: O2k-FluoRespirometer
O2k-Manual: POS Service
Step 1: O2k-Videosupport: OroboPOS from Perspex housing
Step 2: O2k-Videosupport: Cathode cleaning
Step 3: O2k-Videosupport: Anode cleaning
Step 4: O2k-Videosupport: Membrane mounting
Step 5: O2k-Videosupport: DL7 Start DatLab7
Step 6: O2k-Videosupport: DL7 Insert OroboPOS
Step 7: O2k-Videosupport: Insert O2k Chamber
Step 8: O2k-Videosupport: O2k Chamber volume calibration

Part 1.2: DatLab and O₂ calibration

Part 1.3: O₂ instrumental background

SOP: O₂ background

Part 2.1: Biological samples and experimental design

Pathway control and coupling control of mitochondrial respiration:

Sample types,mitochondrial preparations and sample addition:

SUIT protocols, SUITbrowser, DL-Protocols:

Part 2.2: Biological experiment and data analysis

O2k-Advanced

O2k-Applications: Simultaneous determination of O₂ and H₂O₂ fluxes

@@ Line 4: / Line 4: @@
 == General information ==
-:::: To benefit optimally from the tutored training sessions of the Virtual O2k-Workshop, please make yourself familiar with '''Materials for self-study''' in advance.
+:::: To benefit optimally from the tutored training sessions of the O2k-Virtual Support, please make yourself familiar with '''Materials for self-study''' in advance.
 == O2k-Basic ==
@@ Line 19: / Line 19: @@
 :::* Step 8: [[Image:YouTube Play Button.svg .png|20px|link=https://youtu.be/zZI7O5zAruw]] [https://youtu.be/zZI7O5zAruw O2k-Videosupport: O2k Chamber volume calibration]
-=== Part 1.2: DatLab ===
+=== Part 1.2: DatLab and  O<sub>2</sub> calibration ===
 :::* [[MitoPedia: DatLab]]
 :::* [[MiPNet26.06 DatLab 7: Guide|O2k-Manual: DatLab 7 Guide]]
 :::* [[Run DL-Protocol/Set O2 limit|DL-Protocols]]
-=== Part 1.3: O<sub>2</sub> calibration and instrumental background  ===
 :::* [[Gnaiger 2008 POS]]
 :::* [[MiPNet19.03 O2k-cleaning and ISS|SOP: O2k-cleaning and ISS]]
 :::* [[MiPNet06.03 POS-calibration-SOP|SOP: O<sub>2</sub>-calibration]]
 :::* [[Image:YouTube Play Button.svg .png|20px|link=https://youtu.be/onOIVpmOEoE]] [https://youtu.be/onOIVpmOEoE O2k-Videosupport: QC1: POS calibration]
+=== Part 1.3: O<sub>2</sub> instrumental background  ===
 :::* [[MiPNet14.06 Instrumental O2 background|SOP: O<sub>2</sub> background]]
@@ Line 67: / Line 68: @@
 :::* [[MiPNet24.10 H2O2 flux analysis|H<sub>2</sub>O<sub>2</sub> flux analysis]]
 :::* [[Image:YouTube Play Button.svg .png|20px|link=https://youtu.be/_Fv0VSA_c14]]  [https://youtu.be/_Fv0VSA_c14 O2k-Videosupport: H<sub>2</sub>O<sub>2</sub> flux analysis with DatLab 7.4]
-== NextGen-O2k KOL Training ==
-(R) These materials will be sent upon registration
-:::* '''PB-Module Manual''' (R)
-::::* O2k-Videosupport: SUIT-030_PB_ce_D069 demo experiment (R)
-::::* DL-Protocol: SUIT-030_PB_ce_D069 (R)
-::::* O2k-Videosupport: SUIT-030_PB_ce_D070 demo experiment (R)
-::::* DL-Protocol: SUIT-030_PB_ce_D070 (R)
-:::* '''Q-Module'''
-::::* Q-Module manual: [[MiPNet24.12 NextGen-O2k: Q-Module]]
-::::* CV software manual: [[MiPNet24.16 DatLab8.0: CV-Module]]
-::::*Videos: [https://wiki.oroboros.at/index.php/O2k-Videosupport#Q-Module_.28NextGen-O2k_-_DatLab_7.29 Q-Module]